Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,852,854 G→T 100% P801P (CCG→CCT)  mutS → methyl‑directed mismatch repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,852,8540GT99.5% 722.7 / ‑4.4 218P801P (CCG→CCT) mutSmethyl‑directed mismatch repair protein
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (107/110);  total (107/111)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 218 total aligned reads displayed.

CGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTT  >  CP009273/2852710‑2852997
                                                                                                                                                |                                                                                                                                               
  acacCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgc                                                                                                                                            <  2:3030204/148‑1 (MQ=255)
  acacCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgc                                                                                                                                            <  1:1418563/148‑1 (MQ=255)
  acacCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgc                                                                                                                                            >  2:2417912/1‑148 (MQ=255)
    acCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccg                                                                                                                                          >  2:1910280/1‑148 (MQ=255)
     ccATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccgc                                                                                                                                         >  2:2580875/1‑148 (MQ=255)
      cATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccgcc                                                                                                                                        >  2:2934593/1‑148 (MQ=255)
        ttGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAAcgccgccgc                                                                                                                                      >  2:2656087/1‑148 (MQ=255)
         tGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCt                                                                                                                                     <  2:2057012/148‑1 (MQ=255)
            cTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAGGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACg                                                                                                                                  >  2:1632410/1‑148 (MQ=255)
             tttATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGc                                                                                                                                 >  2:1675211/1‑148 (MQ=255)
                   cacaGCGTGCAGGATGGGGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTgg                                                                                                                           >  2:1118930/1‑148 (MQ=255)
                   cacaGCGTGCAGGATGGGGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTgg                                                                                                                           >  2:3129519/1‑148 (MQ=255)
                     caGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGAt                                                                                                                         >  1:208316/1‑148 (MQ=255)
                       gCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATgg                                                                                                                       <  1:836489/148‑1 (MQ=255)
                       gCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATgg                                                                                                                       <  2:1288413/148‑1 (MQ=255)
                        cGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGt                                                                                                                      >  1:3311886/1‑148 (MQ=255)
                          tGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTGATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCACGTGGATGGTAc                                                                                                                    >  2:2376550/1‑148 (MQ=255)
                              ggATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCaa                                                                                                                <  1:120362/148‑1 (MQ=255)
                              ggATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCaa                                                                                                                <  1:120023/148‑1 (MQ=255)
                                 tGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATg                                                                                                             <  2:3373657/148‑1 (MQ=255)
                                   gcgcGGCGAGCAACAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTc                                                                                                           <  2:1311606/148‑1 (MQ=255)
                                   gcgcGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTc                                                                                                           <  2:2673943/148‑1 (MQ=255)
                                   gcgcGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTc                                                                                                           <  2:2674606/148‑1 (MQ=255)
                                    cgcgGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCt                                                                                                          <  1:1583701/148‑1 (MQ=255)
                                       ggcgAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTg                                                                                                       <  2:1612623/148‑1 (MQ=255)
                                           aGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGt                                                                                                   >  1:3093390/1‑148 (MQ=255)
                                            gCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTc                                                                                                  <  1:2535671/148‑1 (MQ=255)
                                             cAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCa                                                                                                 <  1:2417912/148‑1 (MQ=255)
                                             cAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCa                                                                                                 >  2:3440002/1‑148 (MQ=255)
                                               aaaGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGt                                                                                               <  1:1376663/148‑1 (MQ=255)
                                                aaGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTa                                                                                              >  1:1550684/1‑148 (MQ=255)
                                                aaGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTa                                                                                              >  2:9554/1‑148 (MQ=255)
                                                aaGCTACGGCCTGGCGGCTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTa                                                                                              >  2:625578/1‑148 (MQ=255)
                                                 aGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTAc                                                                                             <  1:2914583/148‑1 (MQ=255)
                                                  gCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTAcc                                                                                            >  2:1900104/1‑148 (MQ=255)
                                                  gCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTAcc                                                                                            >  1:1024961/1‑148 (MQ=255)
                                                   cTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCa                                                                                           >  2:1104374/1‑148 (MQ=255)
                                                            ggCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACt                                                                                  <  1:728078/148‑1 (MQ=255)
                                                               ggTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCg                                                                               <  1:2036028/148‑1 (MQ=255)
                                                                gTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGc                                                                              <  2:28838/148‑1 (MQ=255)
                                                                 ttGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGcc                                                                             <  1:2622134/148‑1 (MQ=255)
                                                                    cAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGc                                                                          >  2:1532418/1‑148 (MQ=255)
                                                                     aGCTCTGGCAGGCGTTCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCg                                                                         <  1:2680373/148‑1 (MQ=255)
                                                                     aGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCg                                                                         >  2:2782209/1‑148 (MQ=255)
                                                                     aGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCg                                                                         <  1:926476/148‑1 (MQ=255)
                                                                       ctctGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGt                                                                       <  1:2263771/148‑1 (MQ=255)
                                                                       ctctGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGt                                                                       >  1:2765911/1‑148 (MQ=255)
                                                                         ctGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCg                                                                     >  2:2648582/1‑148 (MQ=255)
                                                                         ctGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCg                                                                     >  1:113878/1‑148 (MQ=255)
                                                                           ggCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGaa                                                                   <  1:1898922/148‑1 (MQ=255)
                                                                           ggCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGaa                                                                   >  2:1064382/1‑148 (MQ=255)
                                                                            gCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAg                                                                  <  2:1344677/148‑1 (MQ=255)
                                                                                gCGTGCCAAAAGAGGTTATTCAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGctct                                                              <  1:1311413/148‑1 (MQ=255)
                                                                                gCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGctct                                                              <  1:3060160/148‑1 (MQ=255)
                                                                                   tGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGa                                                           <  2:1844156/148‑1 (MQ=255)
                                                                                     ccAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGaaa                                                         >  2:1198840/1‑148 (MQ=255)
                                                                                     ccAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGaaa                                                         >  2:2933490/1‑148 (MQ=255)
                                                                                     ccAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGaaa                                                         <  1:255318/148‑1 (MQ=255)
                                                                                       aaaaGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAAt                                                       >  1:1480620/1‑148 (MQ=255)
                                                                                        aaaGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATc                                                      >  1:1058435/1‑148 (MQ=255)
                                                                                        aaaGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATc                                                      <  1:2665632/148‑1 (MQ=255)
                                                                                          agagGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCtt                                                    >  2:3185516/1‑148 (MQ=255)
                                                                                           gagGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTg                                                   >  1:285615/1‑148 (MQ=255)
                                                                                             ggTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGAt                                                 >  2:2772081/1‑148 (MQ=255)
                                                                                                  ttaAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGa                                            <  1:2934595/148‑1 (MQ=255)
                                                                                                  ttaAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGa                                            <  1:1675073/148‑1 (MQ=255)
                                                                                                  ttaAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCATGCGGTCGAAGCTCTGGAAAATCTTGATCCGGa                                            >  2:1584351/1‑148 (MQ=255)
                                                                                                   taAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGAt                                           >  1:41338/1‑148 (MQ=255)
                                                                                                     aGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtc                                         >  2:3217310/1‑148 (MQ=255)
                                                                                                      gcgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAATTGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCAGGATtca                                        <  1:3129843/148‑1 (MQ=255)
                                                                                                      gcgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtca                                        <  1:507256/148‑1 (MQ=255)
                                                                                                      gcgcgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCAGGATtca                                        <  1:1118930/148‑1 (MQ=255)
                                                                                                         cgcACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATtcactc                                     >  2:42153/1‑148 (MQ=255)
                                                                                                            aCGTCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGATACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCAcc                                  <  1:3401405/148‑1 (MQ=255)
                                                                                                            aCGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCAcc                                  <  1:2510235/148‑1 (MQ=255)
                                                                                                               gCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCcg                               <  1:257552/148‑1 (MQ=255)
                                                                                                                  aaaGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGt                            <  1:3065860/148‑1 (MQ=255)
                                                                                                                  aaaGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGt                            >  2:3247905/1‑148 (MQ=255)
                                                                                                                   aaGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTc                           >  2:1384749/1‑148 (MQ=255)
                                                                                                                   aaGCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTc                           >  1:1791204/1‑148 (MQ=255)
                                                                                                                     gCTGCGTGAGCTTGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAg                         >  1:805138/1‑148 (MQ=255)
                                                                                                                     gCTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAg                         <  2:1723426/148‑1 (MQ=255)
                                                                                                                      cTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAgg                        >  1:2372664/1‑148 (MQ=255)
                                                                                                                      cTGCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAgg                        >  2:3017415/1‑148 (MQ=255)
                                                                                                                        gCGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGgcg                      <  1:3192975/148‑1 (MQ=255)
                                                                                                                         cGTGAGCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGgcgc                     <  2:2791680/148‑1 (MQ=255)
                                                                                                                              gCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAg                <  2:2947394/148‑1 (MQ=255)
                                                                                                                              gCTGGAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAg                <  1:94914/148‑1 (MQ=255)
                                                                                                                                 ggAAAGCATTTCGCCTAACGCCGCAGCTACGCAAGTGTATGTTACTCTAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCTGGCGCTGGAGTgg             <  2:1405170/148‑1 (MQ=255)
                                                                                                                                  gAAAGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGa            >  2:2176366/1‑148 (MQ=255)
                                                                                                                                   aaaGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAt           >  1:1011830/1‑148 (MQ=255)
                                                                                                                                   aaaGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAt           >  2:2489466/1‑148 (MQ=255)
                                                                                                                                    aaGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAtt          >  1:78167/1‑148 (MQ=255)
                                                                                                                                     aGCATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGAttt         >  1:2295100/1‑148 (MQ=255)
                                                                                                                                       cATTTCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTAt       >  1:3373284/1‑148 (MQ=255)
                                                                                                                                         tttCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCg     >  1:3502121/1‑148 (MQ=255)
                                                                                                                                          ttCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGc    <  2:1540638/148‑1 (MQ=255)
                                                                                                                                           tCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGATTCTTCGCCTGCGGTCGAAGCTCTGGATAATCTTGATCCGGTTTCACTCAACCCGCGTCTTGCGCTGGAGTGGATTTATCGCt   <  2:1470610/148‑1 (MQ=255)
                                                                                                                                           tCGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCt   <  1:786481/148‑1 (MQ=255)
                                                                                                                                            cGCCTAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCtt  <  1:1068373/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                               
CGACACCATTGCCTTTATGCACAGCGTGCAGGATGGCGCGGCGAGCAAAAGCTACGGCCTGGCGGTTGCAGCTCTGGCAGGCGTGCCAAAAGAGGTTATTAAGCGCGCACGGCAAAAGCTGCGTGAGCTGGAAAGCATTTCGCCGAACGCCGCCGCTACGCAAGTGGATGGTACGCAAATGTCTTTGCTGTCAGTACCAGAAGAAACTTCGCCTGCGGTCGAAGCTCTGGAAAATCTTGATCCGGATTCACTCACCCCGCGTCAGGCGCTGGAGTGGATTTATCGCTT  >  CP009273/2852710‑2852997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑