Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,878,098 T→A 100% A827A (GCA→GCT)  ygcB ← R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,878,0980TA96.0% 625.3 / 12.2 201A827A (GCA→GCT) ygcBR‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity
Reads supporting (aligned to +/- strand):  ref base T (4/4);  new base A (108/84);  total (112/89)
Fisher's exact test for biased strand distribution p-value = 7.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.11e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 201 total aligned reads displayed.

CCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGTGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCGCAAGAATGGTTTCATC  >  CP009273/2877953‑2878241
                                                                                                                                                 |                                                                                                                                               
ccccTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGc                                                                                                                                               >  2:1912200/1‑148 (MQ=255)
ccccTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGc                                                                                                                                               >  1:2396256/1‑148 (MQ=255)
 cccTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCa                                                                                                                                              <  2:2816727/148‑1 (MQ=255)
  ccTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCaa                                                                                                                                             >  2:1663725/1‑148 (MQ=255)
    tGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAgc                                                                                                                                           >  2:790170/1‑148 (MQ=255)
        taGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCt                                                                                                                                       <  1:1113504/148‑1 (MQ=255)
        taGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCt                                                                                                                                       >  1:739597/1‑148 (MQ=255)
          ggTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCa                                                                                                                                     >  1:3418514/1‑148 (MQ=255)
                   aaTACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGTGCAAGCGCCTCATACTGCTGt                                                                                                                            <  2:1695599/148‑1 (MQ=255)
                      aCTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCa                                                                                                                         >  1:833852/1‑148 (MQ=255)
                       cTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCAt                                                                                                                        <  1:1776528/148‑1 (MQ=255)
                       cTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCAt                                                                                                                        >  1:2934024/1‑148 (MQ=255)
                         gTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGa                                                                                                                      <  1:2775896/148‑1 (MQ=255)
                         gTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGa                                                                                                                      >  2:2652279/1‑148 (MQ=255)
                          ttACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACAGTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGAc                                                                                                                     >  1:313907/1‑148 (MQ=255)
                            aCCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACtt                                                                                                                   <  2:820649/148‑1 (MQ=255)
                                tGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGt                                                                                                               >  2:2366798/1‑148 (MQ=255)
                                tGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGt                                                                                                               <  2:1329444/148‑1 (MQ=255)
                                 gCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGTGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTc                                                                                                              <  2:1591122/148‑1 (MQ=255)
                                  ccAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTcc                                                                                                             <  2:52620/148‑1 (MQ=255)
                                     gACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCACAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCg                                                                                                          <  1:323344/148‑1 (MQ=255)
                                      aCCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGt                                                                                                         <  2:2929873/148‑1 (MQ=255)
                                      aCCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGt                                                                                                         <  1:2026249/148‑1 (MQ=255)
                                      aCCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGt                                                                                                         <  2:1604159/148‑1 (MQ=255)
                                         catccatcCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGa                                                                                                      >  2:2022371/1‑148 (MQ=255)
                                          atccatccAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGAc                                                                                                     >  2:1797228/1‑148 (MQ=255)
                                               tccaGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGc                                                                                                >  2:2935297/1‑148 (MQ=255)
                                               tccaGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGc                                                                                                >  1:1551867/1‑148 (MQ=255)
                                                   gATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATc                                                                                            >  2:3174451/1‑148 (MQ=255)
                                                    aTTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCg                                                                                           <  1:218485/148‑1 (MQ=255)
                                                    aTTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCg                                                                                           <  2:1387514/148‑1 (MQ=255)
                                                        tGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCa                                                                                       >  2:2801714/1‑148 (MQ=255)
                                                          tttCCCTTCCAGCCCAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAgt                                                                                     <  1:1038140/148‑1 (MQ=255)
                                                          tttCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAgt                                                                                     >  2:1533153/1‑148 (MQ=255)
                                                          tttCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAgt                                                                                     >  1:2234204/1‑148 (MQ=255)
                                                           ttCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAgtt                                                                                    <  2:2184870/148‑1 (MQ=255)
                                                             cccTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAgttgt                                                                                  <  1:2366798/148‑1 (MQ=255)
                                                              ccTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAgttgtt                                                                                 <  1:2218913/148‑1 (MQ=255)
                                                              ccTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAgttgtt                                                                                 <  1:909166/148‑1 (MQ=255)
                                                               cTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGttt                                                                                <  1:2283624/148‑1 (MQ=255)
                                                                ttCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTa                                                                               <  2:1212749/148‑1 (MQ=255)
                                                                  cccgCCAAAGTAACCCATCTTCATCTACTACTACAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTAcc                                                                             <  1:2707018/145‑1 (MQ=255)
                                                                      ccAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGaa                                                                         >  2:1299175/1‑148 (MQ=255)
                                                                      ccAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGaa                                                                         >  1:675143/1‑148 (MQ=255)
                                                                      ccAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGaa                                                                         >  2:3013674/1‑148 (MQ=255)
                                                                       cAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAg                                                                        <  1:1899072/148‑1 (MQ=255)
                                                                       cAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTGTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAg                                                                        <  2:2085815/148‑1 (MQ=255)
                                                                        aaaGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGa                                                                       <  2:402197/148‑1 (MQ=255)
                                                                        aaaGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGa                                                                       >  2:2696032/1‑148 (MQ=255)
                                                                          aGTAACCCATCTTCATCTACTACTTCAGAAGAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACg                                                                     >  1:2406388/1‑148 (MQ=255)
                                                                           gTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGt                                                                    <  2:2763157/148‑1 (MQ=255)
                                                                           gTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGt                                                                    <  2:2439568/148‑1 (MQ=255)
                                                                             accccAGCTTCATATCCTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGCGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGttt                                                                  <  1:1304861/146‑1 (MQ=255)
                                                                             aaCCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGttt                                                                  >  1:1008261/1‑148 (MQ=255)
                                                                              aCCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTg                                                                 <  2:1671403/148‑1 (MQ=255)
                                                                               cccATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGt                                                                >  2:2505365/1‑148 (MQ=255)
                                                                               cccATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGt                                                                >  2:881316/1‑148 (MQ=255)
                                                                                 cATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTAc                                                              <  1:1797223/148‑1 (MQ=255)
                                                                                 cATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTAc                                                              <  1:1525961/148‑1 (MQ=255)
                                                                                 cATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTAc                                                              <  2:3418514/148‑1 (MQ=255)
                                                                                  aTCTTCATCTACTACTTCCGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTACGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACa                                                             <  1:114722/148‑1 (MQ=255)
                                                                                  aTCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACa                                                             >  1:3039735/1‑148 (MQ=255)
                                                                                   tCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACAt                                                            >  1:801197/1‑148 (MQ=255)
                                                                                   tCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACAt                                                            >  2:2393171/1‑148 (MQ=255)
                                                                                   tCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACAt                                                            >  2:2048674/1‑148 (MQ=255)
                                                                                   tCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACAt                                                            <  2:2313713/148‑1 (MQ=255)
                                                                                    cTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATa                                                           <  1:561868/148‑1 (MQ=255)
                                                                                      tCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAg                                                         <  2:2396256/148‑1 (MQ=255)
                                                                                         actaccaCTCCAGAAAAACGACGTTTCCAGCGGCAGGGTACATTGACGCGATTAAGAGCACGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCa                                                      <  2:3205448/147‑1 (MQ=255)
                                                                                          ctactaCTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCaa                                                     >  2:329244/1‑148 (MQ=255)
                                                                                           tactacTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCaat                                                    <  2:430556/148‑1 (MQ=255)
                                                                                             ctactTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCaataa                                                  >  1:1187468/1‑148 (MQ=255)
                                                                                             ctactTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCaataa                                                  >  1:771274/1‑148 (MQ=255)
                                                                                              tactTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGc                                                                                        <  2:3214242/109‑1 (MQ=255)
                                                                                               actTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATg                                                >  2:783944/1‑148 (MQ=255)
                                                                                               actTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATg                                                >  1:1676129/1‑148 (MQ=255)
                                                                                                ctTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATgg                                               >  1:429938/1‑148 (MQ=255)
                                                                                                  tCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCa                                             >  1:2470360/1‑148 (MQ=255)
                                                                                                    aGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAgg                                           <  2:833852/148‑1 (MQ=255)
                                                                                                     gAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGc                                          >  2:1035669/1‑148 (MQ=255)
                                                                                                      aaaaaCTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCt                                         >  2:1233452/1‑148 (MQ=255)
                                                                                                                   ccAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATcc                            <  1:2047125/148‑1 (MQ=255)
                                                                                                                    cAGGTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATccc                           <  1:329244/148‑1 (MQ=255)
                                                                                                                      ggTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTc                         >  2:1141866/1‑148 (MQ=255)
                                                                                                                       gTGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGTCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCg                        <  1:2098382/148‑1 (MQ=255)
                                                                                                                        tGAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGt                       <  2:1566387/148‑1 (MQ=255)
                                                                                                                         gAAGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGtt                      >  1:3444072/1‑148 (MQ=255)
                                                                                                                          aaGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTa                     >  1:1594285/1‑148 (MQ=255)
                                                                                                                           aGGGTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTAc                    >  2:633212/1‑148 (MQ=255)
                                                                                                                            gggTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTAcc                   >  2:2138322/1‑148 (MQ=255)
                                                                                                                            gggTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTAcc                   >  2:2060397/1‑148 (MQ=255)
                                                                                                                             ggTACATTGACGCGATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCg                  >  1:3320863/1‑148 (MQ=255)
                                                                                                                                       cgcgATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCGCAAGAATGGt        >  1:2657913/1‑148 (MQ=255)
                                                                                                                                       cgcgATTAAGAGCAAGCGCCTCAGACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCGCAAGAATGGt        <  1:3082597/148‑1 (MQ=255)
                                                                                                                                         cgATTAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCGCAAGAATGGttt      <  1:2455513/148‑1 (MQ=255)
                                                                                                                                            ttAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCGCAAGAATGGTTTCAt   <  1:3186645/148‑1 (MQ=255)
                                                                                                                                            ttAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCGCAAGAATGGTTTCAt   >  2:3400173/1‑148 (MQ=255)
                                                                                                                                            ttAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCGCAAGAATGGTTTCAt   >  1:1317345/1‑148 (MQ=255)
                                                                                                                                             tAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCGCAAGAATGGTTTCATc  >  1:2860325/1‑148 (MQ=255)
                                                                                                                                             tAAGAGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCGCAAGAATGGTTTCATc  >  1:3166517/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCCCTGTATAGGTAATAACAATACTGTTACCCTGCCAGACCCATCCATCCAGATTCTGTTTCCCTTCCAGCCAAAGTAACCCATCTTCATCTACTACTTCAGAAAAACTACGTTTCCAGGTGAAGGGTACATTGACGCGATTAAGTGCAAGCGCCTCATACTGCTGTTCATGACTTAGGTCCTCGTAGACCTGGCCATCGAGCAGTTGTTTACCTGAAGACGTTTGTACATAAGGCAATAATGGCAGGCTCATTTCCCCATCCCTCGTTACCGCAAGAATGGTTTCATC  >  CP009273/2877953‑2878241

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑