Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,878,668 G→T 100% V637V (GTC→GTA)  ygcB ← R‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,878,6680GT98.8% 782.2 / 0.3 245V637V (GTC→GTA) ygcBR‑loop helicase‑annealase Cas3 needed for Cascade anti‑viral activity
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base T (112/130);  total (115/130)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

AAATGGTGGCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTAG  >  CP009273/2878523‑2878811
                                                                                                                                                 |                                                                                                                                               
aaaTGGTGGCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTAc                                                                                                                                               >  2:1587275/1‑148 (MQ=255)
aaaTGGTGGCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTAc                                                                                                                                               >  1:931472/1‑148 (MQ=255)
  aTGGTGGCAACAGGAATCTAAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCt                                                                                                                                             <  2:2834826/148‑1 (MQ=255)
   tggtggCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTg                                                                                                                                            <  2:1050934/148‑1 (MQ=255)
   tggtggCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTg                                                                                                                                            >  2:2779776/1‑148 (MQ=255)
     gtggCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTggg                                                                                                                                          <  1:377060/148‑1 (MQ=255)
     gtggCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTggg                                                                                                                                          <  1:381753/148‑1 (MQ=255)
        gCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTg                                                                                                                                       >  1:1494040/1‑148 (MQ=255)
        gCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTg                                                                                                                                       >  1:1494777/1‑148 (MQ=255)
        gCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTg                                                                                                                                       <  1:1689329/148‑1 (MQ=255)
            cAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGAc                                                                                                                                   >  2:635173/1‑148 (MQ=255)
              ggAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACaa                                                                                                                                 >  2:613946/1‑148 (MQ=255)
               gAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAg                                                                                                                                <  2:3434233/148‑1 (MQ=255)
               gAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAg                                                                                                                                >  1:2418817/1‑148 (MQ=255)
                aaTCTCAAAACCAGCGGGACGATATTTGCGATGATGGCTATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGt                                                                                                                               <  2:376254/148‑1 (MQ=255)
                  tctcAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTAt                                                                                                                             <  2:2283014/148‑1 (MQ=255)
                   ctcAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATc                                                                                                                            <  2:528343/148‑1 (MQ=255)
                   ctcAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATc                                                                                                                            >  1:119026/1‑148 (MQ=255)
                    tcAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATcc                                                                                                                           >  1:636381/1‑148 (MQ=255)
                      aaaaCCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGt                                                                                                                         >  1:2583443/1‑148 (MQ=255)
                        aaCCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTcc                                                                                                                       >  2:2967898/1‑148 (MQ=255)
                            aGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACa                                                                                                                   <  2:829782/148‑1 (MQ=255)
                             gCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACAt                                                                                                                  >  2:745354/1‑148 (MQ=255)
                                  aCGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGc                                                                                                             >  1:684518/1‑148 (MQ=255)
                                  aCGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGc                                                                                                             <  2:680494/148‑1 (MQ=255)
                                   cGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCt                                                                                                            >  2:3070392/1‑148 (MQ=255)
                                    gATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCtt                                                                                                           <  2:3325247/148‑1 (MQ=255)
                                    gATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCtt                                                                                                           <  2:2075391/148‑1 (MQ=255)
                                     atatTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTc                                                                                                          <  1:1452803/148‑1 (MQ=255)
                                      tatTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTcc                                                                                                         >  1:2085409/1‑148 (MQ=255)
                                        tttGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAATATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCTTCCAACATTTAGCTTCCCa                                                                                                       >  2:1430909/1‑148 (MQ=255)
                                        tttGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCa                                                                                                       <  1:219693/148‑1 (MQ=255)
                                         ttGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCAt                                                                                                      <  1:2517932/148‑1 (MQ=255)
                                         ttGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCAt                                                                                                      <  1:2517889/148‑1 (MQ=255)
                                            cGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCAtttt                                                                                                   <  1:1110563/148‑1 (MQ=255)
                                              atgatgGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTg                                                                                                 <  1:1763970/148‑1 (MQ=255)
                                                 atgGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCg                                                                                              >  1:898504/1‑148 (MQ=255)
                                                   ggCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGaa                                                                                            <  1:1476548/148‑1 (MQ=255)
                                                   ggCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGaa                                                                                            <  1:3219993/148‑1 (MQ=255)
                                                    gCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGaaa                                                                                           <  1:726838/148‑1 (MQ=255)
                                                         gTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCt                                                                                      <  1:3506543/148‑1 (MQ=255)
                                                         gTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCt                                                                                      <  1:3440433/148‑1 (MQ=255)
                                                          tAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCta                                                                                     <  1:132656/148‑1 (MQ=255)
                                                          tAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCta                                                                                     <  1:3339423/148‑1 (MQ=255)
                                                           aaaCGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCtaa                                                                                    <  1:1406270/148‑1 (MQ=255)
                                                           aaaCGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCtaa                                                                                    <  2:3025925/148‑1 (MQ=255)
                                                            aaCGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCtaat                                                                                   >  2:2718115/1‑148 (MQ=255)
                                                             aCGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCtaata                                                                                  >  2:1795543/1‑148 (MQ=255)
                                                             aCGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAAAATTTCGCTTCCCATTTTTGCCGAAATTGCtaata                                                                                  <  2:2591196/148‑1 (MQ=255)
                                                              cGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCtaataa                                                                                 <  2:896082/148‑1 (MQ=255)
                                                                    aaTCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGAt                                                                           >  1:88004/1‑148 (MQ=255)
                                                                     aTCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGAtt                                                                          <  2:3160140/148‑1 (MQ=255)
                                                                     aTCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGAtt                                                                          <  2:2523687/148‑1 (MQ=255)
                                                                      tCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATtc                                                                         >  1:1245234/1‑148 (MQ=255)
                                                                       cGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATtct                                                                        <  1:2376489/148‑1 (MQ=255)
                                                                        gTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATtctc                                                                       <  2:2418817/148‑1 (MQ=255)
                                                                          tGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCtt                                                                     <  1:520059/148‑1 (MQ=255)
                                                                            gAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAACTTGCTAATAACTCGATTCTCtttt                                                                   >  2:2386282/1‑148 (MQ=255)
                                                                            gAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCtttt                                                                   >  2:1630606/1‑148 (MQ=255)
                                                                             aaaaGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCttttt                                                                  <  1:1251152/148‑1 (MQ=255)
                                                                              aaaGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTc                                                                 <  2:2640614/148‑1 (MQ=255)
                                                                                  cAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCacga                                                             >  2:1603379/1‑148 (MQ=255)
                                                                                     aTCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCAcgacga                                                          <  2:768404/148‑1 (MQ=255)
                                                                                      tCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGAt                                                         >  1:2704412/1‑148 (MQ=255)
                                                                                            ggACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCa                                                   >  1:2220035/1‑148 (MQ=255)
                                                                                               cAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCg                                                >  2:1247018/1‑148 (MQ=255)
                                                                                                  tGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTaa                                             <  1:2317641/148‑1 (MQ=255)
                                                                                                    cTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGATATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAg                                           <  2:1068682/148‑1 (MQ=255)
                                                                                                    cTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAg                                           <  1:1009173/148‑1 (MQ=255)
                                                                                                     tGATTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAgc                                          <  1:339870/148‑1 (MQ=255)
                                                                                                         tAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAgcgcgc                                      >  1:2737922/1‑148 (MQ=255)
                                                                                                          aaTTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCa                                     <  2:442042/148‑1 (MQ=255)
                                                                                                           aTTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCAt                                    >  2:3504974/1‑148 (MQ=255)
                                                                                                            ttAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATg                                   <  1:1577471/148‑1 (MQ=255)
                                                                                                             tAACCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGa                                  >  2:2511230/1‑148 (MQ=255)
                                                                                                               aCCAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGaaa                                >  1:3180921/1‑148 (MQ=255)
                                                                                                                 caaacaaaATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTACCCATTATTGCCGAAATTGCTACTAACTCGATTCTCTATTACACGACGATCGTACAGCGTACAGCGCGCAGCAAACa                              >  2:3480922/1‑148 (MQ=255)
                                                                                                                 cAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACa                              >  2:1325741/1‑148 (MQ=255)
                                                                                                                 cAATCAAAATCAACGTCGAGTGATTGTTCCACTACCTGGGTAGCGACAAGTATCCGACCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAATCGCGCATGAAACa                              >  2:2045277/1‑148 (MQ=255)
                                                                                                                    tcaaaatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAAt                           <  1:591354/148‑1 (MQ=255)
                                                                                                                    tcaaaatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAAt                           <  1:2670178/148‑1 (MQ=255)
                                                                                                                    tcaaaatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAAt                           >  1:2917037/1‑148 (MQ=255)
                                                                                                                       aaatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCta                        <  2:2119413/148‑1 (MQ=255)
                                                                                                                        aatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCtat                       >  2:924275/1‑148 (MQ=255)
                                                                                                                        aatcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCtat                       <  2:232013/148‑1 (MQ=255)
                                                                                                                         atcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTACAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCtata                      >  1:211837/1‑148 (MQ=255)
                                                                                                                          tcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTatat                     <  2:2583443/148‑1 (MQ=255)
                                                                                                                          tcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTatat                     <  1:1158893/148‑1 (MQ=255)
                                                                                                                          tcaaCGTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTatat                     <  1:1627335/148‑1 (MQ=255)
                                                                                                                               gTCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACt                <  1:1830188/148‑1 (MQ=255)
                                                                                                                                tCGAGTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACtt               >  1:857751/1‑148 (MQ=255)
                                                                                                                                    gTGATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCgt           <  2:3387529/148‑1 (MQ=255)
                                                                                                                                      gATTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCgtgt         <  1:1533682/148‑1 (MQ=255)
                                                                                                                                       aTTGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGtt        >  1:423918/1‑148 (MQ=255)
                                                                                                                                         tGTTCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTAt      >  1:3000554/1‑148 (MQ=255)
                                                                                                                                            tCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTa   >  1:805285/1‑148 (MQ=255)
                                                                                                                                            tCCACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTACAGCGTAAAGCGCACATGAAACAAATCTATATCTACTTGCGTGTTATTTa   >  2:1728018/1‑148 (MQ=255)
                                                                                                                                            tCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTa   >  2:2211597/1‑148 (MQ=255)
                                                                                                                                            tCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTa   >  2:1233607/1‑148 (MQ=255)
                                                                                                                                             ccACTACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTAg  <  2:1494774/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AAATGGTGGCAACAGGAATCTCAAAACCAGCGGGACGATATTTGCGATGATGGCGATGTAAACGGCCCAATCGTTGGAAAAGCAAATCTGCAGGACAATGCTGAGTAATTAACCAATCAAAATCAACGTCGAGTGATTGTTCCACGACCTGGGTTGCGACAAGTATCCGTCCAACATTTCGCTTCCCATTTTTGCCGAAATTGCTAATAACTCGATTCTCTTTTTCACGACGATCGTTCAGCGTAAAGCGCGCATGAAACAAATCTATATCTACTTGCGTGTTATTTAG  >  CP009273/2878523‑2878811

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑