Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,945,306 C→G 100% G172A (GGC→GCC)  recD ← exonuclease V (RecBCD complex), alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,945,3060CG99.4% 729.3 / ‑3.8 178G172A (GGC→GCC) recDexonuclease V (RecBCD complex), alpha chain
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (86/91);  total (86/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

AAAGCCTTGCCGAGAGATTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGCCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTCACTTCGT  >  CP009273/2945162‑2945450
                                                                                                                                                |                                                                                                                                                
aaaGCCTTGCCGAGAGATTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGggcgc                                                                                                                                               <  1:1879223/148‑1 (MQ=255)
    ccTTGCCGAGAGATTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGa                                                                                                                                           >  2:2394055/1‑148 (MQ=255)
     cTTGCCGAGAGATTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGaa                                                                                                                                          >  1:1306660/1‑148 (MQ=255)
       tGCCGAGAGATTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAAt                                                                                                                                        <  1:240676/148‑1 (MQ=255)
       tGCCGAGAGATTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAAt                                                                                                                                        <  2:798744/148‑1 (MQ=255)
              agaTTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCgag                                                                                                                                 <  1:505738/148‑1 (MQ=255)
                aTTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGAt                                                                                                                               <  1:3270452/148‑1 (MQ=255)
                aTTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGAt                                                                                                                               <  1:2938141/148‑1 (MQ=255)
                 ttCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATc                                                                                                                              >  1:1221256/1‑148 (MQ=255)
                  tCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGACATCACCGAGATcc                                                                                                                             >  2:1148578/1‑148 (MQ=255)
                     gTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATccgcc                                                                                                                          >  1:1596270/1‑148 (MQ=255)
                      ttAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATccgccg                                                                                                                         >  2:2795844/1‑148 (MQ=255)
                         aGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGt                                                                                                                      <  2:1778384/148‑1 (MQ=255)
                         aGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGt                                                                                                                      <  2:1193337/148‑1 (MQ=255)
                            gcgcgGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAg                                                                                                                   <  1:954721/148‑1 (MQ=255)
                             cgcgGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAgc                                                                                                                  >  1:2743296/1‑148 (MQ=255)
                                 gCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGCCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCa                                                                                                              <  1:650655/148‑1 (MQ=255)
                                    gCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTg                                                                                                           >  2:3051853/1‑148 (MQ=255)
                                    gCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTg                                                                                                           >  2:1944574/1‑148 (MQ=255)
                                     cTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTgc                                                                                                          <  2:2810044/148‑1 (MQ=255)
                                          cccGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGccgccg                                                                                                     >  1:2155224/1‑148 (MQ=255)
                                            cGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCa                                                                                                   <  2:558685/148‑1 (MQ=255)
                                                 gTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACttt                                                                                              <  2:1445496/148‑1 (MQ=255)
                                                  tGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACtttt                                                                                             <  1:2221629/148‑1 (MQ=255)
                                                   gCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTGCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACttttt                                                                                            <  2:531288/148‑1 (MQ=255)
                                                      gCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGcc                                                                                         <  2:1927964/148‑1 (MQ=255)
                                                       ccAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGGCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGccc                                                                                        >  1:45673/1‑147 (MQ=255)
                                                                tACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTc                                                                               <  2:2150584/148‑1 (MQ=255)
                                                                    gCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCg                                                                           <  1:436272/148‑1 (MQ=255)
                                                                     cAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGc                                                                          >  2:3434310/1‑148 (MQ=255)
                                                                      aGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCt                                                                         >  1:3504190/1‑148 (MQ=255)
                                                                       gCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCtt                                                                        <  2:1221256/148‑1 (MQ=255)
                                                                         gTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTAc                                                                      <  1:2788950/148‑1 (MQ=255)
                                                                           tCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTg                                                                    >  2:1349891/1‑148 (MQ=255)
                                                                           tCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTg                                                                    >  1:2445360/1‑148 (MQ=255)
                                                                           tCGCCGTCGGCCAGTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTg                                                                    >  1:1948538/1‑148 (MQ=255)
                                                                            cGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTgg                                                                   >  2:1253210/1‑148 (MQ=255)
                                                                              ccGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGaa                                                                 >  1:3376878/1‑148 (MQ=255)
                                                                              ccGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGaa                                                                 <  1:2204835/148‑1 (MQ=255)
                                                                                gTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGaaaa                                                               <  2:910940/148‑1 (MQ=255)
                                                                                 tCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTTCCAGTTAATTTCATCGCTTACTGGaaaaa                                                              >  1:656929/1‑148 (MQ=255)
                                                                                   ggCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGt                                                            <  2:2254525/148‑1 (MQ=255)
                                                                                    gCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGtt                                                           >  1:1549696/1‑148 (MQ=255)
                                                                                     ccATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGttt                                                          <  1:1139366/148‑1 (MQ=255)
                                                                                     ccATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGttt                                                          >  2:2843567/1‑148 (MQ=255)
                                                                                     ccATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGttt                                                          >  1:1744478/1‑148 (MQ=255)
                                                                                     ccATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGttt                                                          <  1:1835763/148‑1 (MQ=255)
                                                                                      cATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTg                                                         <  1:3288104/148‑1 (MQ=255)
                                                                                      cATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTg                                                         >  1:3025788/1‑148 (MQ=255)
                                                                                       aTTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGt                                                        >  1:1504096/1‑148 (MQ=255)
                                                                                       aTTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGt                                                        >  1:1504193/1‑148 (MQ=255)
                                                                                       aTTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTGACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGt                                                        <  2:2041401/148‑1 (MQ=255)
                                                                                        tttGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTc                                                       >  1:72036/1‑148 (MQ=255)
                                                                                        tttGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTc                                                       >  1:502580/1‑148 (MQ=255)
                                                                                        tttGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTc                                                       >  2:2728296/1‑148 (MQ=255)
                                                                                        tttGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTc                                                       >  2:536913/1‑148 (MQ=255)
                                                                                        tttGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCAAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTAATCGCTTACTGGAAAAAGTTTGTc                                                       >  1:1382458/1‑148 (MQ=255)
                                                                                         ttGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTcc                                                      <  2:1298237/148‑1 (MQ=255)
                                                                                          tGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCa                                                     <  1:3366334/148‑1 (MQ=255)
                                                                                            aaTTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAgg                                                   <  2:2147346/148‑1 (MQ=255)
                                                                                             aTTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAggg                                                  <  2:2964422/148‑1 (MQ=255)
                                                                                             aTTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAggg                                                  <  2:1625624/148‑1 (MQ=255)
                                                                                             aTTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAggg                                                  <  1:801305/148‑1 (MQ=255)
                                                                                              ttAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGt                                                 <  2:2583349/148‑1 (MQ=255)
                                                                                              ttAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGt                                                 >  2:679596/1‑148 (MQ=255)
                                                                                              ttAACGCTGCCAGCAACGTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGt                                                 <  2:3068502/148‑1 (MQ=255)
                                                                                               tAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGtt                                                >  2:74035/1‑148 (MQ=255)
                                                                                               tAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGtt                                                >  2:1801319/1‑148 (MQ=255)
                                                                                                   gCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTgcg                                            >  1:2243887/1‑148 (MQ=255)
                                                                                                          gCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTag                                     <  2:1761007/148‑1 (MQ=255)
                                                                                                          gCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTag                                     <  2:3376878/148‑1 (MQ=255)
                                                                                                          gCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTag                                     <  2:1454854/148‑1 (MQ=255)
                                                                                                          gCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTag                                     >  1:1762954/1‑148 (MQ=255)
                                                                                                          gCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTag                                     >  1:2127491/1‑148 (MQ=255)
                                                                                                             aCTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGc                                  <  2:871157/148‑1 (MQ=255)
                                                                                                             aCTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGc                                  <  1:202791/148‑1 (MQ=255)
                                                                                                             aCTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAAGTTCATGGCTTACTGGAAAAAGTTTGTCCTGGGTTTGCGCCAGTAGAGc                                  >  1:2622205/1‑148 (MQ=255)
                                                                                                                tCGCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTtc                               <  2:3188629/148‑1 (MQ=255)
                                                                                                                  gCTACGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTtcat                             <  2:2009028/148‑1 (MQ=255)
                                                                                                                     aCGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCaa                          >  2:1141215/1‑148 (MQ=255)
                                                                                                                      cGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAAc                         >  1:1116429/1‑148 (MQ=255)
                                                                                                                      cGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAAc                         >  2:3237698/1‑148 (MQ=255)
                                                                                                                      cGGTGGTCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAAc                         >  2:1004065/1‑148 (MQ=255)
                                                                                                                       ggtggtCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAAcc                        <  1:171605/148‑1 (MQ=255)
                                                                                                                          ggtCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCa                     <  2:278492/148‑1 (MQ=255)
                                                                                                                           gtCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCCGGGTTTGCGCCAGTAGAGCTTCATCAACCTCaa                    >  1:1800956/1‑148 (MQ=255)
                                                                                                                            tCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAAt                   <  2:1638773/148‑1 (MQ=255)
                                                                                                                            tCGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAAt                   >  2:1460759/1‑148 (MQ=255)
                                                                                                                             cGTTTTACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATg                  <  1:3099286/148‑1 (MQ=255)
                                                                                                                               ttttACCGGTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGc                <  1:1801325/148‑1 (MQ=255)
                                                                                                                                      ggTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTc         <  2:2341006/148‑1 (MQ=255)
                                                                                                                                      ggTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTc         <  1:2394055/148‑1 (MQ=255)
                                                                                                                                      ggTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTc         <  1:2392350/148‑1 (MQ=255)
                                                                                                                                      ggTGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTc         <  2:814051/148‑1 (MQ=255)
                                                                                                                                        tGCCAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTCAc       >  1:1888379/1‑148 (MQ=255)
                                                                                                                                          ccAGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTCACtt     >  1:1456440/1‑148 (MQ=255)
                                                                                                                                            aGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTCACTTCg   >  2:923540/1‑148 (MQ=255)
                                                                                                                                            aGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTCACTTCg   <  1:1025848/148‑1 (MQ=255)
                                                                                                                                            aGGGGCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTCACTTCg   >  1:2315755/1‑148 (MQ=255)
                                                                                                                                             ggggCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTCACTTCGt  >  1:2786793/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AAAGCCTTGCCGAGAGATTCGGTTAAGCGCGCGGCAGCTTTACCCGTTGGTGCAGCCAGACGGATACGGCAGCGTTCGCCGTCGGCCATTTGAATTAACGCTGCCAGCAACTTCGCTACGGTGGTCGTTTTACCGGTGCCAGGGCCGCCGGAAATCACCGAGATCCGCCGCGTCAGCGCCACTGCCGCCGCAACTTTTTGCCAGTTAATTTCATCGCTTACTGGAAAAAGTTTGTCCAGGGTTTGCGCCAGTAGAGCTTCATCAACCTCAATGGCATGATTCACTTCGT  >  CP009273/2945162‑2945450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑