Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,999,827 C→A 100% D69E (GAC→GAA)  ygeW → putative carbamoyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,999,8270CA99.2% 777.9 / ‑2.1 237D69E (GAC→GAA) ygeWputative carbamoyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/2);  new base A (123/112);  total (123/114)
Fisher's exact test for biased strand distribution p-value = 2.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 237 total aligned reads displayed.

AAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGAC  >  CP009273/2999684‑2999971
                                                                                                                                               |                                                                                                                                                
  aGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTcc                                                                                                                                            <  2:880394/148‑1 (MQ=255)
   gATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCa                                                                                                                                           <  2:451587/148‑1 (MQ=255)
   gATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCa                                                                                                                                           <  2:1469185/148‑1 (MQ=255)
         ttGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTa                                                                                                                                     >  2:1026249/1‑148 (MQ=255)
            ttAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTAccc                                                                                                                                  >  2:3230428/1‑148 (MQ=255)
              aaCGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGc                                                                                                                                <  1:1310196/148‑1 (MQ=255)
               aCGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCt                                                                                                                               <  2:671541/148‑1 (MQ=255)
                   gggAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTtctc                                                                                                                           <  1:2478072/148‑1 (MQ=255)
                   gggAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTtctc                                                                                                                           >  2:731907/1‑148 (MQ=255)
                     gAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCtt                                                                                                                         <  1:1599696/148‑1 (MQ=255)
                        cAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCCCCCGTACCCGCTTCTCTTATg                                                                                                                      <  1:1664541/148‑1 (MQ=255)
                           aCGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCtt                                                                                                                   <  1:1666195/148‑1 (MQ=255)
                                aGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCcgcg                                                                                                              >  1:298027/1‑148 (MQ=255)
                                 gATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCgcgc                                                                                                             <  2:1415163/148‑1 (MQ=255)
                                  aTGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCt                                                                                                            >  1:2690267/1‑148 (MQ=255)
                                      aCTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAAc                                                                                                        >  2:2563019/1‑148 (MQ=255)
                                      aCTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAAc                                                                                                        >  1:3407375/1‑148 (MQ=255)
                                        tGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCt                                                                                                      >  2:40482/1‑148 (MQ=255)
                                        tGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCt                                                                                                      >  2:40650/1‑148 (MQ=255)
                                            aCAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTc                                                                                                  >  1:676294/1‑148 (MQ=255)
                                            aCAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTc                                                                                                  >  1:1116417/1‑148 (MQ=255)
                                              aaGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCgg                                                                                                >  2:2442539/1‑148 (MQ=255)
                                               aGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGc                                                                                               <  1:2730545/148‑1 (MQ=255)
                                                gTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGcc                                                                                              >  1:1936807/1‑148 (MQ=255)
                                                gTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGcc                                                                                              <  1:588133/148‑1 (MQ=255)
                                                 tACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCt                                                                                             >  2:3422417/1‑148 (MQ=255)
                                                       aCGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGcaca                                                                                       >  2:2731260/1‑148 (MQ=255)
                                                        cGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCacaa                                                                                      <  1:227457/148‑1 (MQ=255)
                                                        cGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCacaa                                                                                      <  1:2432767/148‑1 (MQ=255)
                                                            gCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCATATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAg                                                                                  <  1:1734576/148‑1 (MQ=255)
                                                                cagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtct                                                                              <  2:2189922/148‑1 (MQ=255)
                                                                cagcaTTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtct                                                                              <  1:427513/148‑1 (MQ=255)
                                                                cagcaTTAAAAGAACTGCGTGCTGAAAACATCTCAACCAAAGTCTATAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGAACCCGCATCTCTAATGCAACCGCGCTTAACCTGCTCGGCCTTGCACAACAAGAtct                                                                              >  2:322180/1‑148 (MQ=255)
                                                                     ttAAAAGCACTTCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcgacg                                                                         <  2:1272889/148‑1 (MQ=255)
                                                                     ttAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTcgacg                                                                         >  2:2817792/1‑148 (MQ=255)
                                                                        aaaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAg                                                                      >  1:2394928/1‑148 (MQ=255)
                                                                        aaaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAg                                                                      <  1:2563019/148‑1 (MQ=255)
                                                                         aaGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAgg                                                                     >  1:1619300/1‑148 (MQ=255)
                                                                          aGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGc                                                                    >  2:2744946/1‑148 (MQ=255)
                                                                            cACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCaa                                                                  >  2:2444929/1‑148 (MQ=255)
                                                                            cACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCaa                                                                  <  1:2058740/148‑1 (MQ=255)
                                                                              cTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCaa                                                                  >  1:1400739/1‑146 (MQ=255)
                                                                                gCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATca                                                              >  2:967852/1‑148 (MQ=255)
                                                                                gCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATca                                                              <  2:2937380/148‑1 (MQ=255)
                                                                                 cGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATcac                                                             >  1:978694/1‑148 (MQ=255)
                                                                                   tGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACaa                                                           <  2:1728592/148‑1 (MQ=255)
                                                                                    gCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACaaa                                                          >  1:2617756/1‑148 (MQ=255)
                                                                                       gAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCg                                                       <  1:3106959/148‑1 (MQ=255)
                                                                                       gAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCg                                                       <  2:1053950/148‑1 (MQ=255)
                                                                                       gAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCg                                                       <  1:2598152/148‑1 (MQ=255)
                                                                                       gAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCg                                                       <  2:438654/148‑1 (MQ=255)
                                                                                        aaaaCATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGc                                                      <  1:2446369/148‑1 (MQ=255)
                                                                                         aaaCATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCt                                                     >  2:1629510/1‑148 (MQ=255)
                                                                                          aaCATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTc                                                    <  1:2516585/148‑1 (MQ=255)
                                                                                           aCATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCa                                                   <  1:527161/148‑1 (MQ=255)
                                                                                            cATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCAc                                                  >  2:1626367/1‑148 (MQ=255)
                                                                                             aTCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACg                                                 <  2:976783/148‑1 (MQ=255)
                                                                                              tctcAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACgg                                                >  1:2140330/1‑148 (MQ=255)
                                                                                               ctcAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGc                                               <  2:3415566/148‑1 (MQ=255)
                                                                                                  aaCCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGaa                                            <  1:2276726/148‑1 (MQ=255)
                                                                                                  aaCCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGaa                                            >  2:387714/1‑148 (MQ=255)
                                                                                                  aaCCAAAGTCTTTAATAGTGGATTAGGTATATCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTAATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATAGCTCACGGAGaa                                            >  2:387743/1‑148 (MQ=255)
                                                                                                   aCCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGaaa                                           <  1:1092460/148‑1 (MQ=255)
                                                                                                   aCCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGaaa                                           <  1:2844907/148‑1 (MQ=255)
                                                                                                    ccAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAAc                                          >  2:1763966/1‑148 (MQ=255)
                                                                                                    ccAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAAc                                          >  1:1157185/1‑148 (MQ=255)
                                                                                                    ccAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAAc                                          >  2:369086/1‑148 (MQ=255)
                                                                                                     cAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAAcc                                         >  2:1435685/1‑148 (MQ=255)
                                                                                                     cAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAAcc                                         <  2:2653012/148‑1 (MQ=255)
                                                                                                      aaaGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcg                                        >  1:685122/1‑148 (MQ=255)
                                                                                                        aGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtg                                      >  2:2882515/1‑148 (MQ=255)
                                                                                                            tttAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACcgtgcgtg                                  >  2:9216/1‑148 (MQ=255)
                                                                                                             ttAATAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGa                                 >  1:3070721/1‑148 (MQ=255)
                                                                                                                 tAGTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAAcc                             >  2:567553/1‑148 (MQ=255)
                                                                                                                   gTGGATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATGGGTGTCGGCGAAACCGTGCGTGAAACCGc                           <  2:875737/148‑1 (MQ=255)
                                                                                                                     ggATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCa                         >  1:1172190/1‑148 (MQ=255)
                                                                                                                     ggATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCa                         <  1:3252419/148‑1 (MQ=255)
                                                                                                                      gATTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCac                        >  1:1439737/1‑147 (MQ=255)
                                                                                                                       aTTAGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAat                       <  1:1026249/148‑1 (MQ=255)
                                                                                                                          aGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATg                    <  2:2268345/148‑1 (MQ=255)
                                                                                                                          aGGTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATg                    <  2:3070721/148‑1 (MQ=255)
                                                                                                                            gTATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAt                  >  1:12594/1‑148 (MQ=255)
                                                                                                                             tATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtc                 >  2:1569213/1‑148 (MQ=255)
                                                                                                                             tATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtc                 >  2:2770388/1‑148 (MQ=255)
                                                                                                                             tATTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtc                 >  1:1953665/1‑148 (MQ=255)
                                                                                                                              aTTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtct                <  1:1409466/148‑1 (MQ=255)
                                                                                                                              aTTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtct                <  2:1936807/148‑1 (MQ=255)
                                                                                                                              aTTTCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGAtct                <  1:1279575/148‑1 (MQ=255)
                                                                                                                                ttCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTcc              <  1:40482/148‑1 (MQ=255)
                                                                                                                                ttCCGTATTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTcc              >  1:2235187/1‑148 (MQ=255)
                                                                                                                                      aTTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgc        <  1:771450/148‑1 (MQ=255)
                                                                                                                                      aTTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgc        >  2:635164/1‑148 (MQ=255)
                                                                                                                                      aTTCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgc        >  2:1306450/1‑148 (MQ=255)
                                                                                                                                       ttCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgcg       >  1:3254238/1‑148 (MQ=255)
                                                                                                                                       ttCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgcg       >  1:3254037/1‑148 (MQ=255)
                                                                                                                                        tCCGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTgcgc      >  1:1245028/1‑148 (MQ=255)
                                                                                                                                         ccGCGAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGcc     <  1:2934755/148‑1 (MQ=255)
                                                                                                                                          cgcgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCg    <  2:2067229/148‑1 (MQ=255)
                                                                                                                                            cgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGAc  >  1:2665555/1‑148 (MQ=255)
                                                                                                                                            cgAAAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGAc  >  1:2775752/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
AAAGATTTTTTGTTAACGTGGGAACAGACGCCAGATGAACTGAAACAAGTACTGGACGTTGCCGCAGCATTAAAAGCACTGCGTGCTGAAAACATCTCAACCAAAGTCTTTAATAGTGGATTAGGTATTTCCGTATTCCGCGACAACTCCACCCGTACCCGCTTCTCTTATGCTTCCGCGCTTAACCTGCTCGGCCTTGCACAACAAGATCTCGACGAAGGCAAATCACAAATCGCTCACGGCGAAACCGTGCGTGAAACCGCCAATATGATCTCCTTCTGCGCCGAC  >  CP009273/2999684‑2999971

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑