Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,030,181 G→T 100% H429N (CAT→AAT)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,030,1810GT99.5% 654.8 / ‑4.3 204H429N (CAT→AAT) recJssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (127/74);  total (128/76)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 204 total aligned reads displayed.

TTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAA  >  CP009273/3030037‑3030325
                                                                                                                                                |                                                                                                                                                
     gCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCaa                                                                                                                                          >  1:1507597/1‑148 (MQ=255)
       cGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAAc                                                                                                                                        <  1:1777396/148‑1 (MQ=255)
         ggCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACtt                                                                                                                                      <  1:3401376/148‑1 (MQ=255)
          gCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTc                                                                                                                                     >  1:287936/1‑148 (MQ=255)
           cTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCa                                                                                                                                    >  1:392221/1‑148 (MQ=255)
            ttAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAg                                                                                                                                   <  2:1356056/148‑1 (MQ=255)
                cGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGcatc                                                                                                                               >  1:2625559/1‑148 (MQ=255)
                 ggACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGcatca                                                                                                                              <  1:1037428/148‑1 (MQ=255)
                   aCCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATg                                                                                                                            >  2:591073/1‑148 (MQ=255)
                   aCCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATg                                                                                                                            >  2:942249/1‑148 (MQ=255)
                    ccGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGc                                                                                                                           <  1:2662324/148‑1 (MQ=255)
                     cGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGcc                                                                                                                          >  1:74593/1‑148 (MQ=255)
                         tGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAggg                                                                                                                      <  1:3333465/148‑1 (MQ=255)
                          gATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGt                                                                                                                     >  2:500664/1‑148 (MQ=255)
                            tACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCTCCAAACTTCAGCATCATGCCAGGGTag                                                                                                                   >  1:432762/1‑148 (MQ=255)
                               cACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgt                                                                                                                >  1:2414011/1‑148 (MQ=255)
                                 cTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgt                                                                                                              <  2:2859949/148‑1 (MQ=255)
                                 cTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgt                                                                                                              >  1:3224703/1‑148 (MQ=255)
                                      ccTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTa                                                                                                         >  2:3273052/1‑148 (MQ=255)
                                       cTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTaa                                                                                                        >  1:2307739/1‑148 (MQ=255)
                                       cTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTaa                                                                                                        >  2:1615488/1‑148 (MQ=255)
                                        ttGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTTAATTTATCCTCTTCCAGAGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAAt                                                                                                       <  2:829128/148‑1 (MQ=255)
                                           cAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGc                                                                                                    >  2:2117941/1‑148 (MQ=255)
                                            aaTAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCt                                                                                                   <  2:175286/148‑1 (MQ=255)
                                             aTAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTc                                                                                                  >  1:702790/1‑148 (MQ=255)
                                                gCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAg                                                                                               >  2:1709407/1‑148 (MQ=255)
                                                    aGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCa                                                                                           <  2:1507597/148‑1 (MQ=255)
                                                    aGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCa                                                                                           >  1:503831/1‑148 (MQ=255)
                                                     gggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCAt                                                                                          >  1:3260191/1‑148 (MQ=255)
                                                      ggTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATc                                                                                         >  2:689171/1‑148 (MQ=255)
                                                       gTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCa                                                                                        <  1:334383/148‑1 (MQ=255)
                                                          cAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGc                                                                                     >  1:1852774/1‑148 (MQ=255)
                                                            gCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCat                                                                                   >  1:2529617/1‑148 (MQ=255)
                                                            gCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCat                                                                                   >  2:2604458/1‑148 (MQ=255)
                                                            gCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCat                                                                                   >  2:10055/1‑148 (MQ=255)
                                                               aCTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATg                                                                                <  2:2140039/148‑1 (MQ=255)
                                                                cTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGc                                                                               >  1:1306093/1‑148 (MQ=255)
                                                                cTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGc                                                                               >  2:1051529/1‑148 (MQ=255)
                                                                cTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCAAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGAATATGc                                                                               >  2:1613458/1‑148 (MQ=255)
                                                                    gTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGcc                                                                           <  2:1129315/148‑1 (MQ=255)
                                                                    gTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGcc                                                                           >  1:1984180/1‑148 (MQ=255)
                                                                     tAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGccc                                                                          <  2:1791538/148‑1 (MQ=255)
                                                                     tAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGccc                                                                          <  1:1886085/148‑1 (MQ=255)
                                                                     tAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGccc                                                                          <  2:2224541/148‑1 (MQ=255)
                                                                      aaCCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGcccc                                                                         >  1:3114577/1‑148 (MQ=255)
                                                                       aCCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCt                                                                        >  2:1183817/1‑148 (MQ=255)
                                                                         cAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGa                                                                      <  1:1378939/148‑1 (MQ=255)
                                                                          agctCGCCAAACCGTTGTTGAAAGAGTTCGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGTAGCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGTGCATCACGCATATGCAGCCCCTGaa                                                                     >  2:2064904/4‑148 (MQ=255)
                                                                          aGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGaa                                                                     >  1:1972419/1‑148 (MQ=255)
                                                                          aGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGaa                                                                     <  2:1577185/148‑1 (MQ=255)
                                                                            ttCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATg                                                                   <  1:828099/148‑1 (MQ=255)
                                                                             tCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATgg                                                                  >  1:2115012/1‑148 (MQ=255)
                                                                                ccAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGc                                                               >  1:2097493/1‑148 (MQ=255)
                                                                                ccAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGc                                                               >  2:716659/1‑148 (MQ=255)
                                                                                   aaCCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGAc                                                            >  1:1965365/1‑148 (MQ=255)
                                                                                   aaCCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGAc                                                            >  1:189703/1‑148 (MQ=255)
                                                                                   aaCCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGAc                                                            >  2:469127/1‑148 (MQ=255)
                                                                                    aCCGTGGTTGAAAGAGTTTGACTTTATCCTCGACCAGCGACACACCCGCCGCCCTCGCACGACCGCCAAAGGTCAGCATCAGGCCAGGGTAGCGTGTGTCAAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGAcc                                                           <  2:2408181/148‑1 (MQ=255)
                                                                                        ttgttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGaa                                                       >  1:151688/1‑148 (MQ=255)
                                                                                        ttgttgAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGaa                                                       >  2:12447/1‑148 (MQ=255)
                                                                                         tgttgAAAGTTTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGAATCATGCCAGGGTAGAGTGTGTATAATCGCTCCAGCGCATCACGAATATGCAGCCCCTGAATGGAGCGACCGGAAc                                                      <  1:1236090/148‑1 (MQ=255)
                                                                                              aaaGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTc                                                 >  1:1937551/1‑148 (MQ=255)
                                                                                              aaaGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTc                                                 >  1:560251/1‑148 (MQ=255)
                                                                                                agagTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAg                                               >  1:2779007/1‑148 (MQ=255)
                                                                                                agagTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCAGGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAg                                               <  1:2060765/148‑1 (MQ=255)
                                                                                                 gagTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGc                                              >  2:1750727/1‑148 (MQ=255)
                                                                                                 gagTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGc                                              >  1:2067493/1‑148 (MQ=255)
                                                                                                 gagTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGc                                              >  2:2848630/1‑148 (MQ=255)
                                                                                                  agTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCg                                             >  2:2814503/1‑148 (MQ=255)
                                                                                                    tttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTa                                           >  2:2654265/1‑148 (MQ=255)
                                                                                                    tttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTa                                           <  2:701184/148‑1 (MQ=255)
                                                                                                    tttGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTa                                           <  2:53091/148‑1 (MQ=255)
                                                                                                        aaTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGt                                       >  1:474626/1‑148 (MQ=255)
                                                                                                         aTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTc                                      >  2:2463138/1‑148 (MQ=255)
                                                                                                          tttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCa                                     >  2:1540451/1‑148 (MQ=255)
                                                                                                          tttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCa                                     >  2:3122985/1‑148 (MQ=255)
                                                                                                          tttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCa                                     <  1:2218498/148‑1 (MQ=255)
                                                                                                          tttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCa                                     >  1:247443/1‑148 (MQ=255)
                                                                                                           ttATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCAc                                    >  2:3422689/1‑148 (MQ=255)
                                                                                                             aTCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACct                                  >  1:1047815/1‑148 (MQ=255)
                                                                                                              tCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctg                                 <  2:2307739/148‑1 (MQ=255)
                                                                                                              tCCTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctg                                 >  1:2085646/1‑148 (MQ=255)
                                                                                                               ccTCTTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACctgc                                >  1:925856/1‑148 (MQ=255)
                                                                                                                  ctTCCAGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTgg                             >  2:3481288/1‑148 (MQ=255)
                                                                                                                       aGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaa                        <  1:499309/148‑1 (MQ=255)
                                                                                                                       aGCGACAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCaa                        >  1:1503275/1‑148 (MQ=255)
                                                                                                                            cAAACCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTACTCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAAcgcg                   <  2:139716/148‑1 (MQ=255)
                                                                                                                              aaCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGAt                 <  1:2816402/148‑1 (MQ=255)
                                                                                                                               aCCCGCCGCCATCGCATTACCTCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATa                <  2:432762/148‑1 (MQ=255)
                                                                                                                               aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATa                >  1:958469/1‑148 (MQ=255)
                                                                                                                               aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATa                >  1:2179092/1‑148 (MQ=255)
                                                                                                                               aCCCGCCGCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATa                >  1:1397833/1‑148 (MQ=255)
                                                                                                                                cccGCCGCCATCGAATTACCGCCAAATTTCAGCTTGATGCCTGGTTAGTGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTTAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGAGCAAACGCGATaa               <  1:163700/148‑1 (MQ=255)
                                                                                                                                 ccgccgCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAAc              <  2:1968482/148‑1 (MQ=255)
                                                                                                                                 ccgccgCCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAAc              >  2:967008/1‑148 (MQ=255)
                                                                                                                                  cgccgcCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAAcc             >  1:846176/1‑148 (MQ=255)
                                                                                                                                  cgccgcCATCGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAAcc             >  1:2346165/1‑148 (MQ=255)
                                                                                                                                           cGCATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGa    >  1:2703241/1‑148 (MQ=255)
                                                                                                                                             cATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaa  <  2:3226646/148‑1 (MQ=255)
                                                                                                                                             cATTACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGaaa  <  2:2629308/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAATCGCTCCAGCGCATCACGCATATGCAGCCCCTGAATGGAGCGACCGGAACCTTTCAGCGTACCGTCACCTGCTGGCGCAAACGCGATAACCGGACGGTGAAA  >  CP009273/3030037‑3030325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑