Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,030,592 T→A 100% N292Y (AAT→TAT)  recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,030,5920TA99.1% 687.0 / ‑2.6 210N292Y (AAT→TAT) recJssDNA exonuclease, 5' ‑‑> 3'‑specific
Reads supporting (aligned to +/- strand):  ref base T (0/2);  new base A (102/106);  total (102/108)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 212 total aligned reads displayed.

GCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATT  >  CP009273/3030447‑3030736
                                                                                                                                                 |                                                                                                                                                
gCTTCACTTGGCACTCCTCGTTCGATCTCTTAGCGCGACGCGCTTAGCGCATCGAGTTCATGTGCCAGCACGCGCGCTACGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCACTCGGCCGGCAGCATAga                                                                                                                                                <  2:677292/148‑1 (MQ=255)
gCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAga                                                                                                                                                >  1:1284559/1‑148 (MQ=255)
 cTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATagag                                                                                                                                               <  2:3175365/148‑1 (MQ=255)
 cTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATagag                                                                                                                                               <  2:2200948/148‑1 (MQ=255)
  ttCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAgaga                                                                                                                                              <  1:70234/148‑1 (MQ=255)
  ttCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAgaga                                                                                                                                              <  1:2549371/148‑1 (MQ=255)
  ttCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAgaga                                                                                                                                              <  1:3130424/148‑1 (MQ=255)
   tCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGAc                                                                                                                                             >  1:2512464/1‑148 (MQ=255)
   tCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGAc                                                                                                                                             >  2:3319035/1‑148 (MQ=255)
   tCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGAc                                                                                                                                             >  1:751814/1‑148 (MQ=255)
    cAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACg                                                                                                                                            <  1:1579266/148‑1 (MQ=255)
    cAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACg                                                                                                                                            <  2:657610/148‑1 (MQ=255)
     aaTTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGt                                                                                                                                           >  1:1382693/1‑148 (MQ=255)
        ttGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGc                                                                                                                                        >  2:308780/1‑148 (MQ=255)
         tGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGcc                                                                                                                                       <  1:543093/148‑1 (MQ=255)
           cATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGcccc                                                                                                                                     <  2:1852774/148‑1 (MQ=255)
           cATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGcccc                                                                                                                                     >  1:390548/1‑148 (MQ=255)
                    ttCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCaaaa                                                                                                                            >  1:1431625/1‑148 (MQ=255)
                        aTCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTa                                                                                                                        >  1:3273401/1‑148 (MQ=255)
                           tctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCTGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAAt                                                                                                                     >  2:2790789/1‑148 (MQ=255)
                           tctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAAt                                                                                                                     >  1:2653330/1‑148 (MQ=255)
                            ctTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATc                                                                                                                    >  2:1614509/1‑148 (MQ=255)
                             ttttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCg                                                                                                                   >  2:1111784/1‑148 (MQ=255)
                              tttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCGGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAACTCGc                                                                                                                  <  1:649334/148‑1 (MQ=255)
                              tttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGc                                                                                                                  >  1:1098056/1‑148 (MQ=255)
                               ttCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCt                                                                                                                 <  2:1036908/148‑1 (MQ=255)
                                   cgTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTggcg                                                                                                             >  1:124417/1‑148 (MQ=255)
                                    gTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTggcgg                                                                                                            <  1:2240965/148‑1 (MQ=255)
                                    gTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTggcgg                                                                                                            <  1:2240496/148‑1 (MQ=255)
                                      cTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGgcggcg                                                                                                          <  1:2022238/148‑1 (MQ=255)
                                      cTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGgcggcg                                                                                                          >  2:3235602/1‑148 (MQ=255)
                                        ggTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAg                                                                                                        <  1:1881416/148‑1 (MQ=255)
                                         gTTTAGCGCATCGATTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATATAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGt                                                                                                       >  2:1399987/1‑148 (MQ=255)
                                         gTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGt                                                                                                       <  2:745239/148‑1 (MQ=255)
                                         gTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGt                                                                                                       >  2:111903/1‑148 (MQ=255)
                                         gTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGAGAGt                                                                                                       >  2:1632560/1‑148 (MQ=255)
                                           ttAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGttt                                                                                                     >  1:624965/1‑148 (MQ=255)
                                           ttAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGttt                                                                                                     <  1:150493/148‑1 (MQ=255)
                                            tAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGtttt                                                                                                    >  2:2494920/1‑148 (MQ=255)
                                                   tCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCAt                                                                                             >  2:1139323/1‑148 (MQ=255)
                                                    cGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATc                                                                                            <  1:1261873/148‑1 (MQ=255)
                                                    cGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATc                                                                                            <  1:1667637/148‑1 (MQ=255)
                                                         tCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGt                                                                                       >  2:3457906/1‑148 (MQ=255)
                                                          cATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGtt                                                                                      <  2:1073375/148‑1 (MQ=255)
                                                            tttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTg                                                                                    <  1:893944/148‑1 (MQ=255)
                                                            tttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTg                                                                                    >  2:2898180/1‑148 (MQ=255)
                                                            tttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTg                                                                                    <  1:2896032/148‑1 (MQ=255)
                                                             ttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGc                                                                                   <  2:843048/148‑1 (MQ=255)
                                                             ttGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGc                                                                                   <  1:3183920/148‑1 (MQ=255)
                                                               gCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCa                                                                                 <  1:170196/148‑1 (MQ=255)
                                                                ccAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCAc                                                                                <  1:3509078/148‑1 (MQ=255)
                                                                ccAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCAc                                                                                <  1:1773705/148‑1 (MQ=255)
                                                                  aGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACtt                                                                              <  2:2632710/148‑1 (MQ=255)
                                                                  aGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACtt                                                                              <  2:779607/148‑1 (MQ=255)
                                                                  aGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACtt                                                                              <  1:776021/148‑1 (MQ=255)
                                                                      cgcgcgCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAag                                                                          <  2:1287121/148‑1 (MQ=255)
                                                                         gcgcTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAagcag                                                                       <  2:3510926/148‑1 (MQ=255)
                                                                             ttCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCt                                                                   <  1:2962477/148‑1 (MQ=255)
                                                                             ttCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCt                                                                   >  2:2379161/1‑148 (MQ=255)
                                                                              tCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCtt                                                                  <  1:227617/148‑1 (MQ=255)
                                                                              tCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCtt                                                                  <  1:1730137/148‑1 (MQ=255)
                                                                                gCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTACATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTa                                                                <  2:3116180/148‑1 (MQ=255)
                                                                                     tgttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATccc                                                           >  1:1233016/1‑148 (MQ=255)
                                                                                     tgttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATccc                                                           >  1:26874/1‑148 (MQ=255)
                                                                                      gttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATcccc                                                          >  1:3455362/1‑148 (MQ=255)
                                                                                       ttgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCg                                                         >  2:1257859/1‑148 (MQ=255)
                                                                                        tgtCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCgg                                                        >  1:1021677/1‑148 (MQ=255)
                                                                                          tCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGAc                                                      >  1:2559125/1‑148 (MQ=255)
                                                                                            gCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACgg                                                    <  2:2764796/148‑1 (MQ=255)
                                                                                               caacaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCAc                                                 >  1:708462/1‑148 (MQ=255)
                                                                                               caacaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCAc                                                 >  2:1552225/1‑148 (MQ=255)
                                                                                                aacaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACt                                                <  2:707912/148‑1 (MQ=255)
                                                                                                 acaacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACtt                                               <  1:3319035/148‑1 (MQ=255)
                                                                                                  caacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGACACttt                                              <  2:800895/148‑1 (MQ=255)
                                                                                                   aacaGCGCCACACCTACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAACTCGCTGGCGGCGAGATTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTc                                             <  2:2369434/148‑1 (MQ=255)
                                                                                                   aacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTc                                             >  2:62136/1‑148 (MQ=255)
                                                                                                   aacaGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTc                                             <  2:2533872/148‑1 (MQ=255)
                                                                                                     caGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCg                                           <  1:2284305/148‑1 (MQ=255)
                                                                                                     caGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCg                                           >  2:2806037/1‑148 (MQ=255)
                                                                                                     caGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCg                                           >  2:2971633/1‑148 (MQ=255)
                                                                                                           cacaCCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCgg                                     <  2:1982043/148‑1 (MQ=255)
                                                                                                            acacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTTCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGa                                    >  1:3036547/1‑148 (MQ=255)
                                                                                                             cacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCGGGGGGGGGGGGTCCGGCTCGGAt                                   >  2:1606955/1‑148 (MQ=255)
                                                                                                             cacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGAt                                   >  1:2325436/1‑148 (MQ=255)
                                                                                                             cacCGACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCGGGGGGGGGGGGTCGGCACTTTCCGGCTCGGAt                                   >  2:3347249/1‑148 (MQ=255)
                                                                                                                 gACGGACATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGa                               <  1:2390534/148‑1 (MQ=255)
                                                                                                                      aCATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCAt                          <  2:1507305/148‑1 (MQ=255)
                                                                                                                       cATATCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATc                         <  1:3378730/148‑1 (MQ=255)
                                                                                                                        atatCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcc                        >  2:2226241/1‑148 (MQ=255)
                                                                                                                        atatCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcc                        >  2:517590/1‑148 (MQ=255)
                                                                                                                        atatCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcc                        >  2:518069/1‑148 (MQ=255)
                                                                                                                          atCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcccc                      <  2:405763/148‑1 (MQ=255)
                                                                                                                          atCGTCCAGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATcccc                      >  1:2136836/1‑148 (MQ=255)
                                                                                                                                 aGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAgg               >  2:3379226/1‑148 (MQ=255)
                                                                                                                                 aGTCGTCCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAgg               >  2:3262759/1‑148 (MQ=255)
                                                                                                                                   tcgtcCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTc             <  1:3352885/148‑1 (MQ=255)
                                                                                                                                     gtcCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAg           <  2:1990672/148‑1 (MQ=255)
                                                                                                                                      tcCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGa          <  1:1614509/148‑1 (MQ=255)
                                                                                                                                      tcCGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGa          <  1:1111784/148‑1 (MQ=255)
                                                                                                                                        cGGCAGCATAGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAAt        <  1:539879/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCTTCAATTTGCATTCCTTGTTCGATCTCTTTTCGCGTCTGGTTTAGCGCATCGAGTTCATTTGCCAGCACGCGCGCTTCGCCGATGTTGTCGCACAACAACAGCGCCACACCGACGGACATATCGTCCAGTCGTCCGGCAGCATTGAGACGTGGCCCCAGCGCAAAACCTAAATCGCTGGCGGCGAGTTTTTGTGCATCACGGTTTGCCACTTCAAGCAGCGCTTTAATCCCCGGACGGCACTTTCCGGCTCGGATGCGACTCATCCCCTGCCAGGTCAGAATGCGATT  >  CP009273/3030447‑3030736

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑