Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,064,914 G→T 100% I356I (ATC→ATA)  pgk ← phosphoglycerate kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,064,9140GT100.0% 761.7 / NA 243I356I (ATC→ATA) pgkphosphoglycerate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (111/132);  total (111/132)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 245 total aligned reads displayed.

CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGA  >  CP009273/3064769‑3065058
                                                                                                                                                 |                                                                                                                                                
ctctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGatat                                                                                                                                                <  1:2058872/148‑1 (MQ=255)
  ctAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATAttt                                                                                                                                              >  1:3322666/1‑148 (MQ=255)
   tAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATAtttt                                                                                                                                             <  1:3118858/148‑1 (MQ=255)
     aaaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGt                                                                                                                                           <  1:509279/148‑1 (MQ=255)
     aaaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGt                                                                                                                                           <  1:2506480/148‑1 (MQ=255)
      aaGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTc                                                                                                                                          >  1:1883298/1‑148 (MQ=255)
          gcgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCa                                                                                                                                      <  2:2327453/148‑1 (MQ=255)
           cgcTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCGGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCaa                                                                                                                                     <  1:859774/148‑1 (MQ=255)
            gcTGAAACAAGGGCAGGTGTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAAt                                                                                                                                    <  1:2960973/148‑1 (MQ=255)
             cTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATg                                                                                                                                   >  2:2616046/1‑148 (MQ=255)
             cTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATg                                                                                                                                   <  1:1374383/148‑1 (MQ=255)
              tGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGc                                                                                                                                  <  1:168816/148‑1 (MQ=255)
              tGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGc                                                                                                                                  >  2:809627/1‑148 (MQ=255)
              tGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGc                                                                                                                                  >  1:600584/1‑148 (MQ=255)
                aaaCAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCg                                                                                                                                >  2:571255/1‑148 (MQ=255)
                aaaCAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCg                                                                                                                                >  1:536520/1‑148 (MQ=255)
                     aGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAg                                                                                                                           <  2:111508/148‑1 (MQ=255)
                      gggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAgg                                                                                                                          >  1:481365/1‑148 (MQ=255)
                      gggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAgg                                                                                                                          >  1:2245994/1‑148 (MQ=255)
                      gggCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAgg                                                                                                                          <  2:572617/148‑1 (MQ=255)
                           ggTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGAt                                                                                                                     <  2:1540828/148‑1 (MQ=255)
                             tttCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtg                                                                                                                   <  2:2127399/148‑1 (MQ=255)
                             tttCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtg                                                                                                                   >  2:3245440/1‑148 (MQ=255)
                             tttCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtg                                                                                                                   <  1:175017/148‑1 (MQ=255)
                             tttACCTGCCCTGTGATTTTTTACTTCTTATCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtg                                                                                                                   >  2:3244648/1‑148 (MQ=255)
                                ccctgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgctg                                                                                                                >  2:104521/1‑148 (MQ=255)
                                ccctgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgctg                                                                                                                >  2:106569/1‑148 (MQ=255)
                                ccctgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgctg                                                                                                                >  2:1829173/1‑148 (MQ=255)
                                ccctgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgctg                                                                                                                >  2:2417083/1‑148 (MQ=255)
                                ccctgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCAGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATtgctg                                                                                                                >  1:1041244/1‑148 (MQ=255)
                                   tgccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCa                                                                                                             >  2:397401/1‑148 (MQ=255)
                                     ccctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCaga                                                                                                           >  2:482168/1‑148 (MQ=255)
                                      cctgTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCagag                                                                                                          <  1:1243487/148‑1 (MQ=255)
                                        tgtgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgtg                                                                                                        <  2:3481619/148‑1 (MQ=255)
                                         gtgATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAgtgt                                                                                                       >  2:2524997/1‑148 (MQ=255)
                                                tttACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTcgccgcc                                                                                                >  1:1100413/1‑148 (MQ=255)
                                                      tcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGc                                                                                          >  2:2110773/1‑148 (MQ=255)
                                                      tcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGc                                                                                          >  2:1028123/1‑148 (MQ=255)
                                                      tcttAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGc                                                                                          >  1:2585048/1‑148 (MQ=255)
                                                         tAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGAt                                                                                       >  2:158733/1‑148 (MQ=255)
                                                         tAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGAt                                                                                       >  2:1866943/1‑148 (MQ=255)
                                                         tAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGAt                                                                                       <  1:3364849/148‑1 (MQ=255)
                                                         tAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGAt                                                                                       <  1:1624958/148‑1 (MQ=255)
                                                          aGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATg                                                                                      >  1:2400071/1‑148 (MQ=255)
                                                             gcgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACTAATTCGAGGAATGCGCCTACGCCAGTGTAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTTCTGCCAGATTGTCTCAGCCGCCAGCGATGgag                                                                                   <  2:285330/148‑1 (MQ=255)
                                                              cgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGgaga                                                                                  <  1:1167097/148‑1 (MQ=255)
                                                              cgcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGgaga                                                                                  <  1:2895434/148‑1 (MQ=255)
                                                               gcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGaa                                                                                 <  1:474116/148‑1 (MQ=255)
                                                               gcTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGaa                                                                                 <  1:3244880/148‑1 (MQ=255)
                                                                 tctTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACg                                                                               >  2:1512817/1‑148 (MQ=255)
                                                                  ctTCGAGCATCGCTACTGCAGGCAGTACCTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGc                                                                              <  1:1948039/148‑1 (MQ=255)
                                                                   ttCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCt                                                                             <  2:3001885/148‑1 (MQ=255)
                                                                    tCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAAAGTGTCGCCGCCGCCAGCGATGGAGAACGCtt                                                                            >  2:1676669/1‑148 (MQ=255)
                                                                       aGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGc                                                                         >  2:736421/1‑148 (MQ=255)
                                                                         cATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGAAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTg                                                                       >  2:2205599/1‑148 (MQ=255)
                                                                          aTCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGt                                                                      <  1:3063780/148‑1 (MQ=255)
                                                                          aTCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGt                                                                      <  2:356639/148‑1 (MQ=255)
                                                                                 cTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAt                                                               <  1:421839/148‑1 (MQ=255)
                                                                                 cTGCAGGCAGTACTTTACCGTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGAt                                                               <  2:1530524/148‑1 (MQ=255)
                                                                                    caggcagTACTCTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGc                                                            >  1:1731307/1‑148 (MQ=255)
                                                                                       gcagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGtt                                                         >  2:2472302/1‑148 (MQ=255)
                                                                                        cagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTa                                                        <  2:710583/148‑1 (MQ=255)
                                                                                        cagTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTa                                                        <  2:2275652/148‑1 (MQ=255)
                                                                                         agTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAg                                                       <  1:995169/148‑1 (MQ=255)
                                                                                         agTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAg                                                       <  1:1440411/148‑1 (MQ=255)
                                                                                          gTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGc                                                      <  1:809627/148‑1 (MQ=255)
                                                                                           tACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGcc                                                     >  2:221060/1‑148 (MQ=255)
                                                                                            aCTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCa                                                    <  1:66097/148‑1 (MQ=255)
                                                                                            aCTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCa                                                    <  1:3026493/148‑1 (MQ=255)
                                                                                              tttACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACg                                                  >  1:2424539/1‑148 (MQ=255)
                                                                                                tACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGAt                                                >  1:3399840/1‑148 (MQ=255)
                                                                                                   cTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTc                                             >  2:419477/1‑148 (MQ=255)
                                                                                                    ttCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGAGATAGCGTTAGCCACGATTTCa                                            >  1:1639812/1‑148 (MQ=255)
                                                                                                       cACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTa                                         >  2:2300160/1‑148 (MQ=255)
                                                                                                           aaTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCtt                                     <  2:2266721/148‑1 (MQ=255)
                                                                                                           aaTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCtt                                     <  1:119942/148‑1 (MQ=255)
                                                                                                            aTTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCttt                                    >  2:3393469/1‑148 (MQ=255)
                                                                                                            aTTCGAGGAATGCGCCGCCGCCAGGGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCttt                                    >  2:1655162/1‑148 (MQ=255)
                                                                                                             ttCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTg                                   >  1:96181/1‑148 (MQ=255)
                                                                                                                gAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCgg                                <  1:235905/148‑1 (MQ=255)
                                                                                                                gAGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCgg                                <  1:1830537/148‑1 (MQ=255)
                                                                                                                 aGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGa                               <  2:1100413/148‑1 (MQ=255)
                                                                                                                 aGGAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTAAGTACCTTTGCGGa                               <  1:571255/148‑1 (MQ=255)
                                                                                                                  ggAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGaa                              <  2:1210819/148‑1 (MQ=255)
                                                                                                                  ggAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGaa                              <  2:495856/148‑1 (MQ=255)
                                                                                                                   gAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAg                             <  2:205292/148‑1 (MQ=255)
                                                                                                                   gAATGCGCCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAg                             >  1:2673550/1‑148 (MQ=255)
                                                                                                                       gcgcCGCCGCCAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCg                         >  2:1365963/1‑148 (MQ=255)
                                                                                                                          ccgccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGa                      <  2:1942351/148‑1 (MQ=255)
                                                                                                                           cgccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGaa                     <  1:361571/148‑1 (MQ=255)
                                                                                                                           cgccgccAGTGGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGaa                     <  2:2634214/148‑1 (MQ=255)
                                                                                                                                 cAGTCGAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCAGACAGCGTGTAGCCTCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACCCTTTCATTCCCATTGCGGAAGTGCGGGAATTAGaa               >  2:1993795/1‑148 (MQ=255)
                                                                                                                                     ggAGATGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACg           <  1:1842665/148‑1 (MQ=255)
                                                                                                                                       agaTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGcc         <  1:203524/148‑1 (MQ=255)
                                                                                                                                         aTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCaa       >  2:2785445/1‑148 (MQ=255)
                                                                                                                                         aTGTAGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCaa       >  2:789106/1‑148 (MQ=255)
                                                                                                                                             aGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCgg   >  2:3407423/1‑148 (MQ=255)
                                                                                                                                             aGGATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCgg   >  2:3406211/1‑148 (MQ=255)
                                                                                                                                              ggATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGa  >  1:2062271/1‑148 (MQ=255)
                                                                                                                                              ggATATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGa  >  1:2861944/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTCTAAAAGCGCGCTGAAACAAGGGCAGGTTTCCCTGCCCTGTGATTTTTTACTTCTTAGCGCGCTCTTCGAGCATCGCTACTGCAGGCAGTACTTTACCTTCCACGAATTCGAGGAATGCGCCGCCGCCAGTGGAGATGTAGGAGATTTTGTCAGCAATGCCGAACAGGTCGATTGCTGCCAGAGTGTCGCCGCCGCCAGCGATGGAGAACGCTTCGCTGTCTGCGATAGCGTTAGCCACGATTTCAGTACCTTTGCGGAAGTTCGGGAATTCGAACACGCCAACCGGA  >  CP009273/3064769‑3065058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑