Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,618 T→A 100% V185E (GTG→GAG)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,6180TA99.6% 731.3 / ‑4.6 221V185E (GTG→GAG) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (92/128);  total (93/128)
Fisher's exact test for biased strand distribution p-value = 4.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 221 total aligned reads displayed.

GCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT  >  CP009273/3080473‑3080762
                                                                                                                                                 |                                                                                                                                                
gCACATATCACCTTTGCACACCTTCTTGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACCTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGc                                                                                                                                                <  1:398307/148‑1 (MQ=255)
   cctatcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCttt                                                                                                                                             >  1:2817160/1‑148 (MQ=255)
    ctatcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTc                                                                                                                                            <  1:2029968/148‑1 (MQ=255)
     tatcacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTcc                                                                                                                                           >  1:1550199/1‑148 (MQ=255)
        cacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACt                                                                                                                                        >  1:2547305/1‑148 (MQ=255)
        cacctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACt                                                                                                                                        >  1:1830874/1‑148 (MQ=255)
        cacctagGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACt                                                                                                                                        <  2:2797481/148‑1 (MQ=255)
         acctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTc                                                                                                                                       <  2:1369235/148‑1 (MQ=255)
           ctatGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAg                                                                                                                                     >  2:2015082/1‑148 (MQ=255)
               gCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCAct                                                                                                                                 >  2:3427997/1‑148 (MQ=255)
                cacaCCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCCGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCActc                                                                                                                                >  2:3470417/1‑148 (MQ=255)
                cacaCCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCActc                                                                                                                                >  2:2309470/1‑148 (MQ=255)
                  cacCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTg                                                                                                                              >  2:871165/1‑148 (MQ=255)
                  cacCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTg                                                                                                                              >  2:3064536/1‑148 (MQ=255)
                     cGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAag                                                                                                                           <  1:662443/148‑1 (MQ=255)
                       tCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAagag                                                                                                                         <  1:2267477/148‑1 (MQ=255)
                        cTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAgaga                                                                                                                        <  1:402863/148‑1 (MQ=255)
                        cTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAgaga                                                                                                                        <  1:415550/148‑1 (MQ=255)
                         tGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGAt                                                                                                                       <  2:566909/148‑1 (MQ=255)
                         tGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGAt                                                                                                                       <  2:3024082/148‑1 (MQ=255)
                            tACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGa                                                                                                                    >  2:1576182/1‑148 (MQ=255)
                             aCAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGAc                                                                                                                   <  1:338486/148‑1 (MQ=255)
                              cAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGAcc                                                                                                                  >  1:3201230/1‑148 (MQ=255)
                               aGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGACAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCa                                                                                                                 <  1:717202/148‑1 (MQ=255)
                                    cAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAAt                                                                                                            <  2:1494227/148‑1 (MQ=255)
                                    cAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAAt                                                                                                            <  2:54613/148‑1 (MQ=255)
                                    cAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAAt                                                                                                            <  2:129055/148‑1 (MQ=255)
                                      ggCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCg                                                                                                          <  2:2603970/148‑1 (MQ=255)
                                       gCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGc                                                                                                         <  1:2786373/148‑1 (MQ=255)
                                       gCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGc                                                                                                         <  2:2266154/148‑1 (MQ=255)
                                         tGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTg                                                                                                       <  1:3016344/148‑1 (MQ=255)
                                           aaGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCa                                                                                                     <  2:2173466/148‑1 (MQ=255)
                                           aaGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCa                                                                                                     <  1:1068760/148‑1 (MQ=255)
                                              tGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCaaga                                                                                                  >  1:1087564/1‑148 (MQ=255)
                                               gCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCaagaa                                                                                                 <  1:2065048/148‑1 (MQ=255)
                                                 gTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGc                                                                                               <  1:2940270/148‑1 (MQ=255)
                                                   aaaaaCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCgg                                                                                             >  1:1235698/1‑148 (MQ=255)
                                                    aaaaCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGt                                                                                            <  2:2745484/148‑1 (MQ=255)
                                                    aaaaCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGt                                                                                            <  2:330750/148‑1 (MQ=255)
                                                     aaaCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTa                                                                                           <  1:1155271/148‑1 (MQ=255)
                                                      aaCGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTaa                                                                                          >  2:83057/1‑148 (MQ=255)
                                                          gCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGa                                                                                      >  2:1219809/1‑148 (MQ=255)
                                                          gCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGa                                                                                      <  1:842677/148‑1 (MQ=255)
                                                          gCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGa                                                                                      <  1:2664150/148‑1 (MQ=255)
                                                          gCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGa                                                                                      <  1:3299747/148‑1 (MQ=255)
                                                           cACTCTACCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGaa                                                                                     >  2:2568057/1‑148 (MQ=255)
                                                             ctctGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATTCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAaga                                                                                   <  2:2596887/148‑1 (MQ=255)
                                                              tctGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAagag                                                                                  <  2:1101323/148‑1 (MQ=255)
                                                              tctGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAagag                                                                                  <  2:1101404/148‑1 (MQ=255)
                                                               ctGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAgaga                                                                                 <  1:2579622/148‑1 (MQ=255)
                                                               ctGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAgaga                                                                                 <  1:1584758/148‑1 (MQ=255)
                                                                 gCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGatc                                                                               <  1:1031904/148‑1 (MQ=255)
                                                                     tGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGAtcatca                                                                           <  1:1455754/148‑1 (MQ=255)
                                                                        cTGCGCCCGTACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGc                                                                        >  1:80850/1‑148 (MQ=255)
                                                                        cTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGc                                                                        <  2:164749/148‑1 (MQ=255)
                                                                         tGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGcc                                                                       >  2:354299/1‑148 (MQ=255)
                                                                         tGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGcc                                                                       <  2:1830874/148‑1 (MQ=255)
                                                                           cgcCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCaa                                                                     >  2:2527504/1‑148 (MQ=255)
                                                                             cccGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAAtt                                                                   <  2:518318/148‑1 (MQ=255)
                                                                                  aCGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGcc                                                              <  2:2510514/148‑1 (MQ=255)
                                                                                  aCGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGcc                                                              <  2:2510466/148‑1 (MQ=255)
                                                                                     cgAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGc                                                           <  1:1893516/148‑1 (MQ=255)
                                                                                        aaaGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGa                                                        <  2:2445454/148‑1 (MQ=255)
                                                                                        aaaGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGa                                                        >  2:691733/1‑148 (MQ=255)
                                                                                         aaGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGaa                                                       >  1:997781/1‑148 (MQ=255)
                                                                                           gCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATg                                                     >  1:3178194/1‑148 (MQ=255)
                                                                                           gCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATg                                                     >  1:3093661/1‑148 (MQ=255)
                                                                                            ccAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATgg                                                    <  1:646893/148‑1 (MQ=255)
                                                                                            ccAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATgg                                                    <  2:1940211/148‑1 (MQ=255)
                                                                                              aGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCt                                                  <  2:1372421/148‑1 (MQ=255)
                                                                                               ggTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTg                                                 <  2:3395047/148‑1 (MQ=255)
                                                                                                 tGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGAc                                               >  2:1470720/1‑148 (MQ=255)
                                                                                                  gACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACt                                              >  1:1354843/1‑148 (MQ=255)
                                                                                                  gACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACt                                              >  1:515042/1‑148 (MQ=255)
                                                                                                   aCTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACtt                                             <  1:33267/148‑1 (MQ=255)
                                                                                                   aCTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACtt                                             <  1:3470436/148‑1 (MQ=255)
                                                                                                   aCTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACtt                                             <  2:2787693/148‑1 (MQ=255)
                                                                                                    cTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTc                                            >  2:956723/1‑148 (MQ=255)
                                                                                                     ttttCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCt                                           >  2:3135205/1‑148 (MQ=255)
                                                                                                            tATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGccaccc                                    >  1:2168350/1‑147 (MQ=255)
                                                                                                            tATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGccacca                                    <  2:476100/148‑1 (MQ=255)
                                                                                                                acgacgGCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAAtt                                >  1:2882519/1‑148 (MQ=255)
                                                                                                                      gCAAAATCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTtcat                          <  2:276100/148‑1 (MQ=255)
                                                                                                                        aaaaTCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTtcatca                        <  2:1538119/148‑1 (MQ=255)
                                                                                                                           aTCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAAcc                     >  1:2356856/1‑148 (MQ=255)
                                                                                                                           aTCGTTGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAAcc                     >  1:1640821/1‑148 (MQ=255)
                                                                                                                               ttGGTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGAc                 >  2:195997/1‑148 (MQ=255)
                                                                                                                                  gTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCgg              >  1:2017685/1‑148 (MQ=255)
                                                                                                                                  gTATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCgg              <  1:1500322/148‑1 (MQ=255)
                                                                                                                                   tATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGt             <  2:1550241/148‑1 (MQ=255)
                                                                                                                                   tATCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGt             >  1:3085387/1‑148 (MQ=255)
                                                                                                                                     tCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcg           >  1:2581914/1‑148 (MQ=255)
                                                                                                                                     tCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcg           >  1:2581994/1‑148 (MQ=255)
                                                                                                                                     tCGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcg           <  2:3201185/148‑1 (MQ=255)
                                                                                                                                      cGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgt          <  2:829907/148‑1 (MQ=255)
                                                                                                                                      cGATGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgt          <  1:2286193/148‑1 (MQ=255)
                                                                                                                                        aTGCTGTCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGtcgttt        <  1:3064536/148‑1 (MQ=255)
                                                                                                                                              tCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACTGGTCGTTTCGTTAt  <  2:1064668/148‑1 (MQ=255)
                                                                                                                                              tCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAt  <  2:2062719/148‑1 (MQ=255)
                                                                                                                                              tCGAGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAt  <  2:3115880/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCACCTATCACCTATGCACACCGTCTGGTACAGCGTCAGGCTGAAGTGCGTAAAAACGGCACTCTGCCGTGGCTGCGCCCGGACGCGAAAAGCCAGGTGACTTTTCAGTATGACGACGGCAAAATCGTTGGTATCGATGCTGTCGTGCTTTCCACTCAGCACTCTGAAGAGATCGACCAGAAATCGCTGCAAGAAGCGGTAATGGAAGAGATCATCAAGCCAATTCTGCCCGCTGAATGGCTGACTTCTGCCACCAAATTCTTCATCAACCCGACCGGTCGTTTCGTTAT  >  CP009273/3080473‑3080762

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑