Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 236,234 A→T 100% V148E (GTG→GAG)  yafV ← putative NAD(P)‑binding C‑N hydrolase family amidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273236,2340AT98.8% 543.5 / ‑2.3 167V148E (GTG→GAG) yafVputative NAD(P)‑binding C‑N hydrolase family amidase
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base T (76/89);  total (76/91)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 167 total aligned reads displayed.

GCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTG  >  CP009273/236092‑236377
                                                                                                                                              |                                                                                                                                               
gCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGa                                                                                                                                            >  2:2659924/1‑148 (MQ=255)
gCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGa                                                                                                                                            <  1:1022310/148‑1 (MQ=255)
  cGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGaaa                                                                                                                                          <  1:1188525/148‑1 (MQ=255)
    aCGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAAc                                                                                                                                        >  1:513590/1‑148 (MQ=255)
      gcgATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGt                                                                                                                                      <  1:2162260/148‑1 (MQ=255)
       cgATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTa                                                                                                                                     >  1:2866569/1‑148 (MQ=255)
       cgATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTa                                                                                                                                     <  1:1285150/148‑1 (MQ=255)
        gATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTaa                                                                                                                                    <  2:2107684/148‑1 (MQ=255)
         aTTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAg                                                                                                                                   >  2:1377479/1‑148 (MQ=255)
             cATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGt                                                                                                                               >  2:1958942/1‑148 (MQ=255)
              aTCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTa                                                                                                                              >  2:1400267/1‑148 (MQ=255)
                cccGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGc                                                                                                                            >  1:6720/1‑148 (MQ=255)
                 ccGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGca                                                                                                                           <  2:2433290/148‑1 (MQ=255)
                 ccGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGca                                                                                                                           <  2:1551912/148‑1 (MQ=255)
                      acaTACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCa                                                                                                                      >  1:1655173/1‑148 (MQ=255)
                          aCGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCgg                                                                                                                  >  1:3512188/1‑148 (MQ=255)
                           cGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGc                                                                                                                 >  1:2160325/1‑148 (MQ=255)
                            gCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCa                                                                                                                >  1:2025682/1‑148 (MQ=255)
                              cTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCaaa                                                                                                              <  1:837253/148‑1 (MQ=255)
                               tGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCaaaa                                                                                                             <  2:2652646/148‑1 (MQ=255)
                               tGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCaaaa                                                                                                             >  2:321593/1‑148 (MQ=255)
                               tGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCaaaa                                                                                                             <  1:3263660/148‑1 (MQ=255)
                                gATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAAt                                                                                                            <  1:1990136/148‑1 (MQ=255)
                                   tctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACg                                                                                                         >  1:1683309/1‑148 (MQ=255)
                                    ctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGc                                                                                                        <  2:1736306/148‑1 (MQ=255)
                                    ctcAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGc                                                                                                        <  1:2516539/148‑1 (MQ=255)
                                         tCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGcc                                                                                                   >  2:1026064/1‑148 (MQ=255)
                                         tCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGcc                                                                                                   >  2:1027069/1‑148 (MQ=255)
                                         tCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGcc                                                                                                   >  1:1402599/1‑148 (MQ=255)
                                              cgGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGcc                                                                                              >  1:1061948/1‑148 (MQ=255)
                                               gggCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCa                                                                                             <  2:361490/148‑1 (MQ=255)
                                               gggCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCa                                                                                             <  1:1513881/148‑1 (MQ=255)
                                               gggCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCa                                                                                             <  1:1513423/148‑1 (MQ=255)
                                                ggCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCAt                                                                                            >  1:1765797/1‑148 (MQ=255)
                                                   cGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTcc                                                                                         <  1:2774850/148‑1 (MQ=255)
                                                        gCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAAt                                                                                    >  2:1181160/1‑148 (MQ=255)
                                                        gCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAAt                                                                                    >  2:1372963/1‑148 (MQ=255)
                                                        gCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAAt                                                                                    <  2:3078290/148‑1 (MQ=255)
                                                        gCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAAt                                                                                    <  2:3491626/148‑1 (MQ=255)
                                                        gCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAAt                                                                                    >  1:514545/1‑148 (MQ=255)
                                                           aTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCAc                                                                                 <  1:2325521/148‑1 (MQ=255)
                                                           aTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCAc                                                                                 >  2:528241/1‑148 (MQ=255)
                                                           aTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCAc                                                                                 >  2:656979/1‑148 (MQ=255)
                                                           aTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCAc                                                                                 <  2:2430716/148‑1 (MQ=255)
                                                           aTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCAc                                                                                 <  2:2431026/148‑1 (MQ=255)
                                                           aTGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCAc                                                                                 >  1:1298464/1‑148 (MQ=255)
                                                            tgcctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACt                                                                                >  2:2400802/1‑148 (MQ=255)
                                                            tgcctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACt                                                                                >  1:58985/1‑148 (MQ=255)
                                                            tgcctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACt                                                                                <  2:2616624/148‑1 (MQ=255)
                                                              cctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTcg                                                                              <  1:2333420/148‑1 (MQ=255)
                                                              cctgccAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTcg                                                                              >  2:1685343/1‑148 (MQ=255)
                                                                     gTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTg                                                                       <  1:2703988/148‑1 (MQ=255)
                                                                           agagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCt                                                                 <  1:3068456/148‑1 (MQ=255)
                                                                           agagCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCt                                                                 >  2:593825/1‑148 (MQ=255)
                                                                             agCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCttt                                                               >  1:1281775/1‑148 (MQ=255)
                                                                             agCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCttt                                                               <  2:820917/148‑1 (MQ=255)
                                                                              gcgcGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTta                                                              <  2:2927274/148‑1 (MQ=255)
                                                                                  ggAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAAt                                                          <  2:3042468/148‑1 (MQ=255)
                                                                                   gAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATg                                                         <  1:1685928/148‑1 (MQ=255)
                                                                                      cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAg                                                      >  2:1469422/1‑148 (MQ=255)
                                                                                      cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAg                                                      >  2:1469668/1‑148 (MQ=255)
                                                                                      cAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAg                                                      <  2:2866569/148‑1 (MQ=255)
                                                                                        ggCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGAt                                                    <  2:488949/148‑1 (MQ=255)
                                                                                         gCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATg                                                   >  1:505095/1‑148 (MQ=255)
                                                                                         gCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATg                                                   >  2:2082267/1‑148 (MQ=255)
                                                                                         gCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATg                                                   >  2:1906346/1‑148 (MQ=255)
                                                                                         gCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCAACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATg                                                   >  2:1271734/1‑148 (MQ=255)
                                                                                           cAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCt                                                 <  1:2958376/148‑1 (MQ=255)
                                                                                            aGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTc                                                <  2:1655173/148‑1 (MQ=255)
                                                                                              ttGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCAt                                              <  2:2334112/148‑1 (MQ=255)
                                                                                              ttGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCAt                                              <  2:513639/148‑1 (MQ=255)
                                                                                              ttGCCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCAt                                              >  1:1332432/1‑148 (MQ=255)
                                                                                                      gTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATg                                      <  1:1400267/148‑1 (MQ=255)
                                                                                                      gTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATg                                      <  1:629456/148‑1 (MQ=255)
                                                                                                         cAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCgg                                   <  2:1626932/148‑1 (MQ=255)
                                                                                                          aGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGa                                  >  1:2730858/1‑148 (MQ=255)
                                                                                                            ggCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAAc                                >  2:2307290/1‑148 (MQ=255)
                                                                                                            ggCGAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAAc                                >  1:2266288/1‑148 (MQ=255)
                                                                                                               gAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGa                             <  1:2457371/148‑1 (MQ=255)
                                                                                                               gAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGa                             <  2:3512188/148‑1 (MQ=255)
                                                                                                               gAGGTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGa                             <  1:3462290/148‑1 (MQ=255)
                                                                                                                aGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGAt                            <  2:804971/148‑1 (MQ=255)
                                                                                                                 ggTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATg                           <  1:2410587/148‑1 (MQ=255)
                                                                                                                 ggTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATg                           >  1:714187/1‑148 (MQ=255)
                                                                                                                  gTCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGa                          <  2:899285/148‑1 (MQ=255)
                                                                                                                   tCATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGAc                         >  2:252780/1‑148 (MQ=255)
                                                                                                                    cATAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACg                        <  2:1602595/148‑1 (MQ=255)
                                                                                                                     aTAATCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGc                       <  2:560167/148‑1 (MQ=255)
                                                                                                                       aaTCGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCtt                     <  1:3136404/148‑1 (MQ=255)
                                                                                                                          cGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAACTACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATc                  <  2:624110/148‑1 (MQ=255)
                                                                                                                          cGTTGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATc                  >  2:2712038/1‑148 (MQ=255)
                                                                                                                             tGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATa               <  2:1402599/148‑1 (MQ=255)
                                                                                                                             tGAGATTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATa               >  2:1158060/1‑148 (MQ=255)
                                                                                                                              gagaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaa              <  1:2583437/148‑1 (MQ=255)
                                                                                                                               agaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaa             >  2:582457/1‑148 (MQ=255)
                                                                                                                                gaTTGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATaaaa            >  1:2871223/1‑148 (MQ=255)
                                                                                                                                  ttGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAAt          >  1:734241/1‑148 (MQ=255)
                                                                                                                                  ttGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAAt          >  1:505915/1‑148 (MQ=255)
                                                                                                                                   tGCGCGACCACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATg         >  2:2547652/1‑148 (MQ=255)
                                                                                                                                          ccACTCAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTg  <  1:2152939/148‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                               
GCCGACGCGATTGCATCCCGCCACATACGCCTGATTCTCAATCGCGCGGGCCGTCAGCAATGCCTGCCAGTGCAGAGAGCGCGGAGCAGGCCAGTTGGCGACGTACAGGGCGAGGTCATAATCGTTGAGATTGCGCGACCACACAGGAAAACGTAAGTCGTAGCACACCAGCGGCAAAATACGCCAGCCGCGCCATTCCACAATCACTCGCGCATTGCCCGCTTTATAATGTAGATGCTCATCTGCCATGCGGAACAGATGACGCTTATCATAAAAATGTACCGTG  >  CP009273/236092‑236377

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑