Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,122,356 G→T 100% L242F (TTG→TTT)  glcC → glycolate‑inducible glc operon transcriptional repressor; autorepressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,122,3560GT99.2% 814.4 / ‑2.1 249L242F (TTG→TTT) glcCglycolate‑inducible glc operon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (98/149);  total (100/149)
Fisher's exact test for biased strand distribution p-value = 1.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 250 total aligned reads displayed.

AAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGA  >  CP009273/3122212‑3122500
                                                                                                                                                |                                                                                                                                                
aaaaaaGCAGATCGATCGCCAGGATGCGCGGTTCTACATCGCGGTGTTGCAGCGGATGCCGCACGTCGCCCAGCGCGGAGCTCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGTAGTCCACCATTTTAtt                                                                                                                                               <  2:2046464/148‑1 (MQ=255)
 aaaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTc                                                                                                                                              >  2:570981/1‑148 (MQ=255)
  aaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCg                                                                                                                                             <  1:404480/148‑1 (MQ=255)
  aaaaGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCg                                                                                                                                             <  1:596905/148‑1 (MQ=255)
     aGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTc                                                                                                                                          >  1:3216430/1‑148 (MQ=255)
      gCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcg                                                                                                                                         >  1:568192/1‑148 (MQ=255)
         gatcgatcGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcggcg                                                                                                                                      >  2:2858655/1‑148 (MQ=255)
         gatcgatcGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcggcg                                                                                                                                      <  2:2686927/148‑1 (MQ=255)
          atcgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTcggcgg                                                                                                                                     >  1:3261660/1‑148 (MQ=255)
           tcgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGt                                                                                                                                    >  1:2102342/1‑148 (MQ=255)
            cgatcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTg                                                                                                                                   <  2:460414/148‑1 (MQ=255)
               tcgCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCg                                                                                                                                >  1:1161218/1‑148 (MQ=255)
                   cAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTgg                                                                                                                            >  1:283558/1‑148 (MQ=255)
                     gCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGgag                                                                                                                          <  2:2073582/148‑1 (MQ=255)
                     gCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGgag                                                                                                                          <  1:1947377/148‑1 (MQ=255)
                     gCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGgag                                                                                                                          <  2:3199234/148‑1 (MQ=255)
                       aTGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGAt                                                                                                                        <  1:1774859/148‑1 (MQ=255)
                         gcgcGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGa                                                                                                                      >  2:2667528/1‑148 (MQ=255)
                           gcgGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAAc                                                                                                                    <  1:955245/148‑1 (MQ=255)
                            cgGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAAcc                                                                                                                   >  2:520756/1‑148 (MQ=255)
                             ggATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCt                                                                                                                  >  1:3336560/1‑148 (MQ=255)
                                tCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAg                                                                                                               <  2:2915498/148‑1 (MQ=255)
                                     aaCGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGc                                                                                                          <  1:1133367/148‑1 (MQ=255)
                                     aaCGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGc                                                                                                          <  1:2884659/148‑1 (MQ=255)
                                         cgGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGt                                                                                                      <  1:3499012/148‑1 (MQ=255)
                                          ggTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTa                                                                                                     <  1:991509/148‑1 (MQ=255)
                                          ggTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTa                                                                                                     <  1:2745566/148‑1 (MQ=255)
                                           gtgtTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTAt                                                                                                    <  1:1860718/148‑1 (MQ=255)
                                           gtgtTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTAt                                                                                                    <  1:1633066/148‑1 (MQ=255)
                                             gtTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTa                                                                                                  >  2:264750/1‑148 (MQ=255)
                                                gCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAATGAATCTCCTCGATATCGAGCTGGAAGGCCAGAATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAAt                                                                                               <  1:1554658/148‑1 (MQ=255)
                                                gCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAAt                                                                                               <  1:2905742/148‑1 (MQ=255)
                                                gCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAAt                                                                                               <  2:2890260/148‑1 (MQ=255)
                                                   gCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTg                                                                                            >  1:1458094/1‑148 (MQ=255)
                                                    cGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGc                                                                                           <  1:832555/148‑1 (MQ=255)
                                                      gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGGCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCAACATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCtt                                                                                         >  2:1286866/1‑148 (MQ=255)
                                                      gCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCAACATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCtt                                                                                         >  2:2875543/1‑148 (MQ=255)
                                                           cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCAt                                                                                    <  1:3089497/148‑1 (MQ=255)
                                                           cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCAt                                                                                    <  1:447577/148‑1 (MQ=255)
                                                           cGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCAt                                                                                    <  2:1833031/148‑1 (MQ=255)
                                                              aCGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACa                                                                                 <  2:1692891/148‑1 (MQ=255)
                                                                gTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAAt                                                                               <  1:442607/148‑1 (MQ=255)
                                                                gTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAAt                                                                               <  1:1502028/148‑1 (MQ=255)
                                                                gTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAAt                                                                               >  1:205522/1‑148 (MQ=255)
                                                                gTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAAt                                                                               <  1:2404802/148‑1 (MQ=255)
                                                                 tCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATc                                                                              >  1:587601/1‑148 (MQ=255)
                                                                 tCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATc                                                                              >  2:3263579/1‑148 (MQ=255)
                                                                   gCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCgg                                                                            <  1:2161066/148‑1 (MQ=255)
                                                                    cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                           >  2:2397596/1‑148 (MQ=255)
                                                                    cccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGt                                                                           >  2:161818/1‑148 (MQ=255)
                                                                     ccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTa                                                                          <  2:1589917/148‑1 (MQ=255)
                                                                     ccAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTa                                                                          <  2:1147579/148‑1 (MQ=255)
                                                                        gcgcgcAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACg                                                                       >  1:1676966/1‑148 (MQ=255)
                                                                         cgcgcAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGc                                                                      <  2:394611/148‑1 (MQ=255)
                                                                           cgcAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCtt                                                                    <  2:1430644/148‑1 (MQ=255)
                                                                            gcagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTg                                                                   <  2:2649030/148‑1 (MQ=255)
                                                                            gcagcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTg                                                                   <  2:730778/148‑1 (MQ=255)
                                                                              agcaCGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTa                                                                 <  2:2050502/148‑1 (MQ=255)
                                                                                  cgcgATCATGTGCGGACCGTGAAAAAGAATCTCCACGCTATCGAGCTGGAAGGCCAACATTTTATTCGCTCGGCGGTGCCGCTGGAGATGACCCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGc                                                             <  2:1342176/148‑1 (MQ=255)
                                                                                     gATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTcc                                                          >  2:2805769/1‑148 (MQ=255)
                                                                                       tCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGc                                                        <  2:2640394/148‑1 (MQ=255)
                                                                                        cATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                       >  2:2432968/1‑148 (MQ=255)
                                                                                        cATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGcc                                                       <  1:2538091/148‑1 (MQ=255)
                                                                                           gtgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCa                                                    <  1:530215/148‑1 (MQ=255)
                                                                                           gtgCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCa                                                    <  2:3396302/148‑1 (MQ=255)
                                                                                             gCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGAAACGCTTGTACGGCTCCGCCCCAAt                                                  >  2:3242292/1‑148 (MQ=255)
                                                                                                gACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCg                                               >  2:1555494/1‑148 (MQ=255)
                                                                                                 aCCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGt                                              <  2:2695427/148‑1 (MQ=255)
                                                                                                   cGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTc                                            <  2:853629/148‑1 (MQ=255)
                                                                                                   cGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTc                                            <  1:2270034/148‑1 (MQ=255)
                                                                                                    gTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTcc                                           >  1:519648/1‑148 (MQ=255)
                                                                                                      gAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAg                                         >  1:663247/1‑148 (MQ=255)
                                                                                                        aaaaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCa                                       <  1:1737267/148‑1 (MQ=255)
                                                                                                         aaaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCAt                                      >  1:1069813/1‑148 (MQ=255)
                                                                                                          aaGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATg                                     >  2:1492110/1‑148 (MQ=255)
                                                                                                           aGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGt                                    >  2:1455591/1‑148 (MQ=255)
                                                                                                            gAATCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGtt                                   >  2:2379674/1‑148 (MQ=255)
                                                                                                             aaTCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTa                                  >  2:2552275/1‑148 (MQ=255)
                                                                                                              aTCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTAt                                 <  2:1892031/148‑1 (MQ=255)
                                                                                                              aTCTCCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCAGGTTAt                                 >  2:3024731/1‑148 (MQ=255)
                                                                                                                ctcCACTATATCGAGCTGGAAGGCCACCCTTTTATTCGCTCGGCGGTGCCGGTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTc                               <  2:893075/148‑1 (MQ=255)
                                                                                                                ctcCACGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTc                               <  1:1560541/148‑1 (MQ=255)
                                                                                                                     cGATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGcc                          <  1:265787/148‑1 (MQ=255)
                                                                                                                      gATATCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCt                         <  1:2907414/148‑1 (MQ=255)
                                                                                                                        tatCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCGGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGt                       >  2:2438571/1‑148 (MQ=255)
                                                                                                                         atCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTa                      <  2:1587820/148‑1 (MQ=255)
                                                                                                                          tCGAGCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTAt                     <  1:882572/148‑1 (MQ=255)
                                                                                                                              gCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCCTACAATCGGTAACGCTTGTACGGCTACGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGt                 <  1:1889878/148‑1 (MQ=255)
                                                                                                                              gCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGt                 <  1:3381957/148‑1 (MQ=255)
                                                                                                                              gCTGGAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGt                 <  1:251953/148‑1 (MQ=255)
                                                                                                                                 ggAAGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTc              >  2:3133013/1‑148 (MQ=255)
                                                                                                                                   aaGGCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGa            <  1:2436920/148‑1 (MQ=255)
                                                                                                                                     ggCCACCATTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAgg          <  2:2207642/148‑1 (MQ=255)
                                                                                                                                         accaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATcc      <  1:1912873/148‑1 (MQ=255)
                                                                                                                                         accaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATcc      <  1:225921/148‑1 (MQ=255)
                                                                                                                                         accaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATcc      <  2:1161218/148‑1 (MQ=255)
                                                                                                                                          ccaTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCa     >  2:2719964/1‑148 (MQ=255)
                                                                                                                                            aTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATg   <  1:3023885/148‑1 (MQ=255)
                                                                                                                                            aTTTTATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATg   >  1:2073002/1‑148 (MQ=255)
                                                                                                                                             ttttATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGa  <  1:1361803/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AAAAAAGCAGATCGATCGCCAGCATGCGCGGATCTACAACGCGGTGTTGCAGCGGCTGCCGCACGTCGCCCAGCGCGCAGCACGCGATCATGTGCGGACCGTGAAAAAGAATCTCCACGATATCGAGCTGGAAGGCCACCATTTGATTCGCTCGGCGGTGCCGCTGGAGATGAACCTGAGTTAGCTGGTATTAAATCTGCTTTTCATACAATCGGTAACGCTTGTACGGCTCCGCCCCAATGCGTTCCAGCATGTTATTCATGCCTGTATTGGTTTCGAGGATCCATGA  >  CP009273/3122212‑3122500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑