Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,134,528 C→A 100% D40Y (GAT→TAT)  hybD ← maturation protease for hydrogenase 2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,134,5280CA97.9% 723.0 / 6.3 236D40Y (GAT→TAT) hybDmaturation protease for hydrogenase 2
Reads supporting (aligned to +/- strand):  ref base C (5/0);  new base A (131/100);  total (136/100)
Fisher's exact test for biased strand distribution p-value = 7.44e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 238 total aligned reads displayed.

TGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCG  >  CP009273/3134384‑3134672
                                                                                                                                                |                                                                                                                                                
tgGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATagag                                                                                                                                               >  1:2952041/1‑148 (MQ=255)
    aaaCAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtc                                                                                                                                           <  2:1545263/148‑1 (MQ=255)
     aacaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtct                                                                                                                                          >  2:159275/1‑148 (MQ=255)
     aacaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtct                                                                                                                                          <  2:1649175/148‑1 (MQ=255)
     aacaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtct                                                                                                                                          >  2:1931142/1‑148 (MQ=255)
      acaacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGAtctc                                                                                                                                         >  2:317152/1‑148 (MQ=255)
       caacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCa                                                                                                                                        <  2:2520362/148‑1 (MQ=255)
       caacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCAGTCCCGCCGTGCCGCCATAGAGGATCTCa                                                                                                                                        <  2:2574510/148‑1 (MQ=255)
        aacGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCaa                                                                                                                                       <  2:3421060/148‑1 (MQ=255)
          cGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACa                                                                                                                                     <  2:312491/148‑1 (MQ=255)
          cGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACa                                                                                                                                     >  2:1583576/1‑148 (MQ=255)
          cGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACa                                                                                                                                     >  1:3207239/1‑148 (MQ=255)
            ccGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACACTGGCATCCGCAATAATCCAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGCTCTCAACATa                                                                                                                                   >  2:3241176/1‑148 (MQ=255)
            ccGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATa                                                                                                                                   <  2:22566/148‑1 (MQ=255)
            ccGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATa                                                                                                                                   <  2:1801304/148‑1 (MQ=255)
             cGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATaa                                                                                                                                  <  2:3306026/148‑1 (MQ=255)
                aaCTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATcc                                                                                                                               >  1:1092011/1‑148 (MQ=255)
                   ttcttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGc                                                                                                                            <  1:2503633/148‑1 (MQ=255)
                     cttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAg                                                                                                                          >  2:1266023/1‑148 (MQ=255)
                     cttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAg                                                                                                                          >  2:1266360/1‑148 (MQ=255)
                     cttcATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAAGCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCAGTCCCGCCCTGCCGCCATAGAGGATCTCAACATAATCCGGCAg                                                                                                                          >  1:3360331/1‑148 (MQ=255)
                        cATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAAt                                                                                                                       >  1:2845131/1‑148 (MQ=255)
                         aTCCCGCAGGATCATCATCGTTCCCGGCGNGTTCTTTTTCGAGACAATGGCATCCGCAATNATCAAATGATCGCGATTTNCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATg                                                                                                                      >  2:2519174/1‑148 (MQ=255)
                         aTCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATg                                                                                                                      >  2:1363667/1‑148 (MQ=255)
                           cccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTa                                                                                                                    <  1:2868301/148‑1 (MQ=255)
                            ccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTAt                                                                                                                   >  1:678486/1‑148 (MQ=255)
                            ccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTAt                                                                                                                   >  2:2791322/1‑148 (MQ=255)
                            ccGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTAt                                                                                                                   <  1:3046484/148‑1 (MQ=255)
                             cGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATc                                                                                                                  >  1:169185/1‑148 (MQ=255)
                             cGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATc                                                                                                                  >  1:168747/1‑148 (MQ=255)
                                aGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGtt                                                                                                               >  2:216369/1‑148 (MQ=255)
                                 ggATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTg                                                                                                              >  1:1341350/1‑148 (MQ=255)
                                 ggATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTg                                                                                                              <  1:1546618/148‑1 (MQ=255)
                                 ggATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTg                                                                                                              >  2:2807473/1‑148 (MQ=255)
                                  gATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGc                                                                                                             >  1:1940616/1‑148 (MQ=255)
                                     catcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGctct                                                                                                          >  2:2694889/1‑148 (MQ=255)
                                     catcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGGTGctct                                                                                                          >  2:2941561/1‑148 (MQ=255)
                                       tcatcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTaa                                                                                                        <  2:2084414/148‑1 (MQ=255)
                                       tcatcGTTCCCGGCGCGTTATTTTTAGAGACAAAGGCATCCGCAATAATAAAATGATCCCGATTTGCCAAGTCGCCAAGCAGCTCCAATCCCGCCGTGCCGCCCTAGAGGATCTCAACATAATACGGCAGAATGTATCCTTGCGCTaa                                                                                                        >  1:1904989/1‑148 (MQ=255)
                                         atcGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACg                                                                                                      <  2:568987/148‑1 (MQ=255)
                                           cGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCt                                                                                                    >  2:2962568/1‑148 (MQ=255)
                                           cGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCt                                                                                                    >  2:1401586/1‑148 (MQ=255)
                                             ttCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTc                                                                                                  >  1:1132243/1‑148 (MQ=255)
                                               cccGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTGGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGa                                                                                                <  2:1904989/148‑1 (MQ=255)
                                                    cgcgTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATc                                                                                           <  2:2579933/148‑1 (MQ=255)
                                                        ttCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGca                                                                                       >  2:2280001/1‑148 (MQ=255)
                                                         tCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGGTGCTCTAACGCTTCGACAATCCGcac                                                                                      >  2:1763998/1‑148 (MQ=255)
                                                              ttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATATAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGa                                                                                 <  2:3086743/148‑1 (MQ=255)
                                                              ttCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGa                                                                                 >  1:3205375/1‑148 (MQ=255)
                                                                 gagaCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATgg                                                                              <  2:519872/148‑1 (MQ=255)
                                                                  agaCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGc                                                                             >  2:884132/1‑148 (MQ=255)
                                                                  agaCAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGc                                                                             >  1:1021405/1‑148 (MQ=255)
                                                                     cAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTc                                                                          >  1:83247/1‑148 (MQ=255)
                                                                      aaTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCa                                                                         <  1:2397347/148‑1 (MQ=255)
                                                                      aaTGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTGCGACAATCCGCACACCGATGGCTTCa                                                                         >  2:1227265/1‑148 (MQ=255)
                                                                         ggCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCg                                                                      >  2:921479/1‑148 (MQ=255)
                                                                           cATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGt                                                                    <  1:2621353/148‑1 (MQ=255)
                                                                             tCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCa                                                                  >  2:586671/1‑148 (MQ=255)
                                                                              ccGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAg                                                                 >  1:2408461/1‑148 (MQ=255)
                                                                               cGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGc                                                                >  2:959039/1‑148 (MQ=255)
                                                                                  aataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaaa                                                             <  1:1724407/148‑1 (MQ=255)
                                                                                   ataatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCaaaa                                                            >  1:3499082/1‑148 (MQ=255)
                                                                                     aatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGTCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAata                                                          >  1:2864438/1‑148 (MQ=255)
                                                                                     aatCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAata                                                          <  2:2629990/148‑1 (MQ=255)
                                                                                        cAACTGAACGCGATTTGCCATGTCGCCAAGCACCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAAGCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                       <  2:2052075/148‑1 (MQ=255)
                                                                                        cAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTg                                                       <  1:509222/148‑1 (MQ=255)
                                                                                            tGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGa                                                   >  2:1460406/1‑148 (MQ=255)
                                                                                               tCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAccc                                                <  1:2280001/148‑1 (MQ=255)
                                                                                               tCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAccc                                                <  1:514943/148‑1 (MQ=255)
                                                                                               tCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAccc                                                <  2:3038017/148‑1 (MQ=255)
                                                                                               tCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAccc                                                <  2:2168909/148‑1 (MQ=255)
                                                                                                cgcgATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGAcccc                                               >  2:2542910/1‑148 (MQ=255)
                                                                                                     tttGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGa                                          <  1:1388684/148‑1 (MQ=255)
                                                                                                     tttGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTAATCGGTCAGCAAAATATTGCCGACCCCTAAGa                                          >  2:1323701/1‑148 (MQ=255)
                                                                                                        gCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTa                                       >  2:3211090/1‑148 (MQ=255)
                                                                                                         ccATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTaa                                      <  1:2729299/148‑1 (MQ=255)
                                                                                                         ccATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTaa                                      >  2:1484436/1‑148 (MQ=255)
                                                                                                          cATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTaaa                                     <  1:2164930/148‑1 (MQ=255)
                                                                                                          cATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTaaa                                     >  2:1724803/1‑148 (MQ=255)
                                                                                                             gTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATa                                  >  2:1957504/1‑148 (MQ=255)
                                                                                                             gTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATa                                  <  1:646737/148‑1 (MQ=255)
                                                                                                                   aaGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAtt                            <  1:219792/148‑1 (MQ=255)
                                                                                                                   aaGCAGCTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCAtt                            <  1:219638/148‑1 (MQ=255)
                                                                                                                    agcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa                           <  1:1401586/148‑1 (MQ=255)
                                                                                                                    agcagcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTa                           <  1:2962568/148‑1 (MQ=255)
                                                                                                                        gcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGa                       <  2:168747/148‑1 (MQ=255)
                                                                                                                        gcTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGa                       <  2:169185/148‑1 (MQ=255)
                                                                                                                         cTCCATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGaa                      >  2:2871816/1‑148 (MQ=255)
                                                                                                                           ccATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAAcc                    <  2:1035832/148‑1 (MQ=255)
                                                                                                                            cATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGACCCt                   >  2:3404494/1‑148 (MQ=255)
                                                                                                                            cATTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCt                   <  2:441170/148‑1 (MQ=255)
                                                                                                                             aTTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt                  >  1:1936710/1‑148 (MQ=255)
                                                                                                                             aTTCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCtt                  <  1:1583576/148‑1 (MQ=255)
                                                                                                                               tCCCGCCGTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCa                <  1:1857083/148‑1 (MQ=255)
                                                                                                                                  cgccgTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTg             <  1:68244/148‑1 (MQ=255)
                                                                                                                                      gTGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAAc         >  1:3476093/1‑148 (MQ=255)
                                                                                                                                       tGCCGCCATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAAcc        >  2:525557/1‑148 (MQ=255)
                                                                                                                                          cgccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACt     <  2:3130969/148‑1 (MQ=255)
                                                                                                                                            ccATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTc   >  2:2760920/1‑148 (MQ=255)
                                                                                                                                             cATAGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCg  >  2:2361528/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TGGTAAACAACGCCGGAACTTCTTCATCCCGCAGGATCATCATCGTTCCCGGCGCGTTCTTTTTCGAGACAATGGCATCCGCAATAATCAAATGATCGCGATTTGCCATGTCGCCAAGCAGCTCCATTCCCGCCGTGCCGCCATCGAGGATCTCAACATAATCCGGCAGAATGTATCGTTGCTCTAACGCTTCGACAATCCGCACACCGATGGCTTCATCGGTCAGCAAAATATTGCCGACCCCTAAGACTAAAATACGCATTACAGAACCTTCACTGAAACCACTTCG  >  CP009273/3134384‑3134672

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑