Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 237,417 A→C 100% F792V (TTT→GTT)  fadE ← acyl coenzyme A dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273237,4170AC99.6% 854.0 / ‑5.4 210F792V (TTT→GTT) fadEacyl coenzyme A dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (92/117);  total (93/117)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 210 total aligned reads displayed.

AAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCTTT  >  CP009273/237272‑237561
                                                                                                                                                 |                                                                                                                                                
aaaaCCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCGTCAACTTTCCGCACTTTCTCCGGCAACTGTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGt                                                                                                                                                >  1:326921/1‑148 (MQ=255)
     cATCCGGATGGCTTTAATTTTAAATAATTAGCGGATACAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGtcatca                                                                                                                                           <  2:2535059/148‑1 (MQ=255)
      aTCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCaa                                                                                                                                          <  1:243466/148‑1 (MQ=255)
      aTCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCaa                                                                                                                                          <  1:320557/148‑1 (MQ=255)
       tCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAAc                                                                                                                                         >  2:895867/1‑148 (MQ=255)
        ccGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACg                                                                                                                                        >  2:2834541/1‑148 (MQ=255)
        ccGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACg                                                                                                                                        >  1:2655467/1‑148 (MQ=255)
         cGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGt                                                                                                                                       <  2:3367631/148‑1 (MQ=255)
          ggATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGtt                                                                                                                                      >  2:1642153/1‑148 (MQ=255)
            aTGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGGGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTaa                                                                                                                                    <  1:3329633/148‑1 (MQ=255)
            aTGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCTCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTaa                                                                                                                                    <  2:3431595/148‑1 (MQ=255)
             tGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAAt                                                                                                                                   >  1:323830/1‑148 (MQ=255)
              gtcTTTAATTTTAAATACTTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATa                                                                                                                                  <  2:2207619/146‑1 (MQ=255)
              ggCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATa                                                                                                                                  <  1:2077610/148‑1 (MQ=255)
               gCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATAc                                                                                                                                 >  1:1855442/1‑148 (MQ=255)
                  ttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTgc                                                                                                                              >  2:2048415/1‑148 (MQ=255)
                  ttAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTgc                                                                                                                              >  2:629125/1‑148 (MQ=255)
                   tAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTgcg                                                                                                                             >  2:3294738/1‑148 (MQ=255)
                      ttttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAg                                                                                                                          <  1:255049/148‑1 (MQ=255)
                      ttttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAg                                                                                                                          <  1:2477480/148‑1 (MQ=255)
                       tttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGa                                                                                                                         >  1:60827/1‑148 (MQ=255)
                       tttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGa                                                                                                                         >  2:3412391/1‑148 (MQ=255)
                        ttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGAc                                                                                                                        <  2:1633067/148‑1 (MQ=255)
                        ttAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGAc                                                                                                                        >  1:283150/1‑148 (MQ=255)
                           aataatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGc                                                                                                                     >  2:2507950/1‑148 (MQ=255)
                           aataatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGc                                                                                                                     >  2:1795/1‑148 (MQ=255)
                            ataatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGTATCAACGTCATCAACGTTAATACTGCGCAGTCGGcg                                                                                                                    >  1:1579994/1‑147 (MQ=255)
                              aatTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCttt                                                                                                                  <  1:2504617/148‑1 (MQ=255)
                               atTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTttc                                                                                                                 >  2:776273/1‑148 (MQ=255)
                               atTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTttc                                                                                                                 >  2:775614/1‑148 (MQ=255)
                                 tAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTttctt                                                                                                               <  1:3439944/148‑1 (MQ=255)
                                 tAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTttctt                                                                                                               >  2:2263959/1‑148 (MQ=255)
                                    cGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAg                                                                                                            <  1:928120/148‑1 (MQ=255)
                                      gATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCt                                                                                                          <  1:613664/148‑1 (MQ=255)
                                       aTAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCtt                                                                                                         <  2:2448127/148‑1 (MQ=255)
                                        tAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                                                                                                        >  2:3350515/1‑148 (MQ=255)
                                        tAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                                                                                                        >  1:3510605/1‑148 (MQ=255)
                                        tAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                                                                                                        >  2:1463247/1‑148 (MQ=255)
                                        tAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCttt                                                                                                        >  2:1380006/1‑148 (MQ=255)
                                         aaaGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTc                                                                                                       <  1:1144270/148‑1 (MQ=255)
                                          aaGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCa                                                                                                      <  2:2328601/148‑1 (MQ=255)
                                           aGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCAc                                                                                                     <  2:3234476/148‑1 (MQ=255)
                                               aCGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGa                                                                                                 <  1:2022412/148‑1 (MQ=255)
                                                 ggAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAAt                                                                                               <  1:682426/148‑1 (MQ=255)
                                                  gAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATa                                                                                              <  1:1260419/148‑1 (MQ=255)
                                                   aGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAg                                                                                             <  2:639356/148‑1 (MQ=255)
                                                   aGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAg                                                                                             >  1:3512286/1‑148 (MQ=255)
                                                    gCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGc                                                                                            >  2:2969244/1‑148 (MQ=255)
                                                     ccTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGACTAGCg                                                                                           >  1:2798630/1‑148 (MQ=255)
                                                      cTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCgg                                                                                          >  1:2859400/1‑148 (MQ=255)
                                                       tttCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGc                                                                                         <  2:60827/148‑1 (MQ=255)
                                                        gtCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCt                                                                                        <  2:1981471/147‑1 (MQ=255)
                                                          cGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTc                                                                                      <  1:948708/148‑1 (MQ=255)
                                                             cTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATc                                                                                   >  2:1081573/1‑148 (MQ=255)
                                                             cTCCGTTATTCATTTACGCGGCTTCAACGTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATc                                                                                   <  2:2461470/148‑1 (MQ=255)
                                                              tCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCt                                                                                  >  2:1025687/1‑148 (MQ=255)
                                                               ccGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCtt                                                                                 >  1:1797764/1‑148 (MQ=255)
                                                               ccGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCtt                                                                                 >  1:2743033/1‑148 (MQ=255)
                                                               ccGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCtt                                                                                 <  1:3375275/148‑1 (MQ=255)
                                                                cGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCttt                                                                                <  2:42349/148‑1 (MQ=255)
                                                                cGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCttt                                                                                <  2:577738/148‑1 (MQ=255)
                                                                cGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCttt                                                                                >  1:573611/1‑148 (MQ=255)
                                                                  ttATTCCTTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTat                                                                              <  2:2632985/148‑1 (MQ=255)
                                                                      tCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTatcaat                                                                          <  1:2226193/148‑1 (MQ=255)
                                                                        aTTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTatcaatca                                                                        >  1:2221135/1‑148 (MQ=255)
                                                                             cgcgGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGcccc                                                                   <  2:1817116/148‑1 (MQ=255)
                                                                              gcgGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCt                                                                  <  1:1408250/148‑1 (MQ=255)
                                                                               cgGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCtt                                                                 <  1:1307731/148‑1 (MQ=255)
                                                                                 gCTTCCACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGATAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCa                                                               <  2:3233427/148‑1 (MQ=255)
                                                                                        cTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAgcgc                                                        >  2:2589151/1‑148 (MQ=255)
                                                                                        cTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAgcgc                                                        >  1:1928413/1‑148 (MQ=255)
                                                                                         tttCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGcgcg                                                       <  2:2306048/148‑1 (MQ=255)
                                                                                              gCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTtgtg                                                  >  2:1919919/1‑148 (MQ=255)
                                                                                              gCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTtgtg                                                  <  1:628953/148‑1 (MQ=255)
                                                                                              gCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTtgtg                                                  <  1:1642153/148‑1 (MQ=255)
                                                                                               cACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTgc                                                 <  1:635426/148‑1 (MQ=255)
                                                                                                aCTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTgcg                                                <  1:1865379/148‑1 (MQ=255)
                                                                                                  tttCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGcc                                              >  1:78286/1‑148 (MQ=255)
                                                                                                    tctcCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAg                                            <  2:399237/148‑1 (MQ=255)
                                                                                                       ccGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTc                                         <  1:2938216/148‑1 (MQ=255)
                                                                                                          gCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATc                                      <  1:218444/148‑1 (MQ=255)
                                                                                                           cAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATcc                                     >  2:1314345/1‑148 (MQ=255)
                                                                                                             aCTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAg                                   <  1:2397761/148‑1 (MQ=255)
                                                                                                             aCTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAg                                   <  1:1325757/148‑1 (MQ=255)
                                                                                                                 tACCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACgg                               <  1:2371207/148‑1 (MQ=255)
                                                                                                                  aCCGGCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACggg                              >  1:830256/1‑148 (MQ=255)
                                                                                                                     ggCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTaa                           >  2:3005175/1‑148 (MQ=255)
                                                                                                                      gCTTCGTCGCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTaaa                          <  1:473462/148‑1 (MQ=255)
                                                                                                                          cgtcgCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGc                      <  1:3350515/148‑1 (MQ=255)
                                                                                                                          cgtcgCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGc                      >  2:1579316/1‑148 (MQ=255)
                                                                                                                           gtcgCCAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCa                     <  1:746565/148‑1 (MQ=255)
                                                                                                                               ccAGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCCGCTTTCACCAGAATCGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGtt                 <  1:2003348/148‑1 (MQ=255)
                                                                                                                                 aGCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCCCCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGtttt               <  1:970111/148‑1 (MQ=255)
                                                                                                                                  gCTCTTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGttttt              >  1:17396/1‑148 (MQ=255)
                                                                                                                                   ctctTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCCGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTa             <  1:324392/148‑1 (MQ=255)
                                                                                                                                     ctTCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTAcc           <  2:3510605/148‑1 (MQ=255)
                                                                                                                                      ttCCGGATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTAccc          >  1:2598658/1‑148 (MQ=255)
                                                                                                                                          ggATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGct      <  2:1554708/148‑1 (MQ=255)
                                                                                                                                           gATCAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTNTCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGctc     >  1:1783079/1‑148 (MQ=255)
                                                                                                                                              cAACGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCttt  <  1:156717/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AAAACCATCCGGATGGCTTTAATTTTAAATAATTAGCGGATAAAGAAACGGAGCCTTTCGGCTCCGTTATTCATTTACGCGGCTTCAACTTTCCGCACTTTCTCCGGCAACTTTACCGGCTTCGTCGCCAGCTCTTCCGGATCAAAGTCATCAACGTTAATACTGCGCAGACGGCTTTCTTCAGCTTTCACCAGAATAGCGGCTTCATCTTTATCAATCAGCCCCTTCACCAGCGCGTTGTGCGCCAGTTCATCCAGACGGGTAAACGGCAGGTTTTTACCCAGCTCTTT  >  CP009273/237272‑237561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑