Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,186,181 G→C 94.2% W205C (TGG→TGC)  yqiJ → DUF1449 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,186,1810GC94.2% 850.3 / 31.9 221W205C (TGG→TGC) yqiJDUF1449 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (6/7);  new base C (107/101);  total (113/108)
Fisher's exact test for biased strand distribution p-value = 7.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 222 total aligned reads displayed.

GCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTC  >  CP009273/3186036‑3186325
                                                                                                                                                 |                                                                                                                                                
   aaGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCa                                                                                                                                             >  2:932084/1‑148 (MQ=255)
    aGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCaa                                                                                                                                            <  1:2123306/148‑1 (MQ=255)
       aaaaCTCACCGATCTATTTTTCCAAATACATTATTTTCTACTGGAGCATGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCGAAata                                                                                                                                         <  1:2085153/148‑1 (MQ=255)
       aaaaCTCACCGATCAATTTGTCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAata                                                                                                                                         <  2:2022420/148‑1 (MQ=255)
       aaaaCTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAata                                                                                                                                         <  1:3090644/148‑1 (MQ=255)
       aaaaCTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAata                                                                                                                                         >  2:2751340/1‑148 (MQ=255)
          aCTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTg                                                                                                                                      >  2:1483103/1‑148 (MQ=255)
          aCTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTg                                                                                                                                      >  2:2163136/1‑148 (MQ=255)
            tCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTa                                                                                                                                    <  1:2112214/148‑1 (MQ=255)
             cACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTaa                                                                                                                                   <  2:2794516/148‑1 (MQ=255)
                 gATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGTAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATtta                                                                                                                               <  2:1170084/148‑1 (MQ=255)
                 gATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATtta                                                                                                                               <  2:1936819/148‑1 (MQ=255)
                  aTCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATttat                                                                                                                              >  2:955573/1‑148 (MQ=255)
                   tCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATttatt                                                                                                                             >  1:2740187/1‑148 (MQ=255)
                       tttGGCAAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATc                                                                                                                         >  2:449046/1‑148 (MQ=255)
                        ttGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCa                                                                                                                        >  2:334119/1‑148 (MQ=255)
                         tGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCaa                                                                                                                       <  1:154744/148‑1 (MQ=255)
                         tGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCaa                                                                                                                       <  1:1928520/148‑1 (MQ=255)
                          ggCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCaaa                                                                                                                      <  2:2319891/148‑1 (MQ=255)
                             cAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAgg                                                                                                                   <  2:1737324/148‑1 (MQ=255)
                               aaTACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGaa                                                                                                                 <  2:3128416/148‑1 (MQ=255)
                                 tACATTATTGACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATAATTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAAt                                                                                                               <  1:1630612/148‑1 (MQ=255)
                                  aCATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAAtt                                                                                                              >  1:1463417/1‑148 (MQ=255)
                                   cATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAAttt                                                                                                             <  1:3327333/148‑1 (MQ=255)
                                     ttattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaa                                                                                                           <  1:2794162/148‑1 (MQ=255)
                                      tattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaat                                                                                                          <  2:745959/148‑1 (MQ=255)
                                         ttACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTaataat                                                                                                       >  2:3069540/1‑148 (MQ=255)
                                          tactacTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATg                                                                                                      <  2:562292/148‑1 (MQ=255)
                                           actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATgg                                                                                                     <  1:539992/148‑1 (MQ=255)
                                           actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATgg                                                                                                     <  1:3494727/148‑1 (MQ=255)
                                            ctactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGga                                                                                                    <  1:1750270/148‑1 (MQ=255)
                                            ctactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGga                                                                                                    <  1:1400994/148‑1 (MQ=255)
                                              actGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatg                                                                                                  >  2:2932932/1‑148 (MQ=255)
                                               ctGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatga                                                                                                 >  1:2427297/1‑148 (MQ=255)
                                                tGGAGCCTGAAGAAGGAAAAATTTTCACTACAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatgat                                                                                                <  2:2474804/148‑1 (MQ=255)
                                                 ggAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGata                                                                                               <  1:1694096/148‑1 (MQ=255)
                                                  gAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGatat                                                                                              <  2:2386216/148‑1 (MQ=255)
                                                   aGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATAtt                                                                                             >  2:2510770/1‑148 (MQ=255)
                                                   aGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATAtt                                                                                             <  2:2808335/148‑1 (MQ=255)
                                                   aGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATAtt                                                                                             >  2:3298101/1‑148 (MQ=255)
                                                    gCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTg                                                                                            >  2:3490586/1‑148 (MQ=255)
                                                     ccTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGt                                                                                           <  2:227529/148‑1 (MQ=255)
                                                     ccTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGt                                                                                           <  2:1223950/148‑1 (MQ=255)
                                                       tGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGGGTTAATTATTTGCCGACTTTCTGCAACGCGATATATGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATATATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTa                                                                                         >  1:832738/1‑148 (MQ=255)
                                                        gaagaaGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTaa                                                                                        <  2:939119/148‑1 (MQ=255)
                                                        gaagaaGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTaa                                                                                        >  1:2157609/1‑148 (MQ=255)
                                                         aagaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAAt                                                                                       <  1:2903363/148‑1 (MQ=255)
                                                         aagaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAAt                                                                                       <  2:2171507/148‑1 (MQ=255)
                                                          agaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAAtt                                                                                      >  1:985002/1‑148 (MQ=255)
                                                          agaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAAtt                                                                                      >  1:2854803/1‑148 (MQ=255)
                                                           gaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTc                                                                                     >  1:3186351/1‑148 (MQ=255)
                                                           gaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTc                                                                                     >  1:939736/1‑148 (MQ=255)
                                                           gaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTc                                                                                     >  1:269293/1‑148 (MQ=255)
                                                           gaagGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTc                                                                                     <  1:385596/148‑1 (MQ=255)
                                                              ggAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTACTTCtgt                                                                                  >  1:2008253/1‑148 (MQ=255)
                                                                 aaaaTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGcc                                                                               <  1:1234403/148‑1 (MQ=255)
                                                                    aTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctc                                                                            >  2:1455637/1‑148 (MQ=255)
                                                                    aTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctc                                                                            >  2:1456298/1‑148 (MQ=255)
                                                                            tAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGt                                                                    >  2:805347/1‑148 (MQ=255)
                                                                             aaaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGtt                                                                   <  2:1912183/148‑1 (MQ=255)
                                                                              aaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGttt                                                                  <  1:1998283/148‑1 (MQ=255)
                                                                                ggAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTAc                                                                <  1:1108332/148‑1 (MQ=255)
                                                                                ggAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTAc                                                                >  2:2696095/1‑148 (MQ=255)
                                                                                ggAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTAc                                                                <  1:1308395/148‑1 (MQ=255)
                                                                                 gagaCAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTAcc                                                               >  2:296984/1‑148 (MQ=255)
                                                                                    aCAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCa                                                            >  1:2273518/1‑148 (MQ=255)
                                                                                    aCAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCa                                                            >  1:507951/1‑148 (MQ=255)
                                                                                           gtTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTg                                                     >  1:1998934/1‑148 (MQ=255)
                                                                                            ttAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGc                                                    <  2:2424772/148‑1 (MQ=255)
                                                                                               attatgTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGt                                                 <  2:1022032/148‑1 (MQ=255)
                                                                                                 tattTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTAt                                               >  2:1807503/1‑148 (MQ=255)
                                                                                                    ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCa                                            >  1:3275373/1‑148 (MQ=255)
                                                                                                    ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCa                                            <  2:298586/148‑1 (MQ=255)
                                                                                                       ccGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTc                                         <  2:3340891/148‑1 (MQ=255)
                                                                                                         gACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTg                                       <  2:1434120/148‑1 (MQ=255)
                                                                                                           cTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGtt                                     >  1:1027498/1‑148 (MQ=255)
                                                                                                           cTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGtt                                     >  1:3172004/1‑148 (MQ=255)
                                                                                                              tCTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttat                                  <  1:19567/148‑1 (MQ=255)
                                                                                                               cTGCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttatt                                 <  2:3511224/148‑1 (MQ=255)
                                                                                                                 gCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTtattat                               <  1:1762926/148‑1 (MQ=255)
                                                                                                                 gCAACGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGGTtattat                               >  1:2768433/1‑148 (MQ=255)
                                                                                                                    aCGCGATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTgg                            >  2:628422/1‑148 (MQ=255)
                                                                                                                     cgcgATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGa                           >  2:762965/1‑148 (MQ=255)
                                                                                                                       cgATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAat                         <  1:239119/148‑1 (MQ=255)
                                                                                                                        gATATTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAatt                        <  2:517808/148‑1 (MQ=255)
                                                                                                                         atatTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAatta                       >  1:357327/1‑148 (MQ=255)
                                                                                                                           atTTGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAattatt                     <  1:2496545/148‑1 (MQ=255)
                                                                                                                            tttGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTAttt                    <  1:147032/148‑1 (MQ=255)
                                                                                                                             ttGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTAtttt                   >  1:2219672/1‑148 (MQ=255)
                                                                                                                             ttGGCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGACGTATGCATTCTGTTTATTATAGGAATTAtttt                   >  1:2936253/1‑148 (MQ=255)
                                                                                                                               ggCGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCg                 >  2:3031670/1‑148 (MQ=255)
                                                                                                                                 cGGAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGcc               <  1:1132192/148‑1 (MQ=255)
                                                                                                                                  ggAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCTCCa              >  2:3263677/1‑148 (MQ=255)
                                                                                                                                   gAAAATAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAg             >  1:655966/1‑148 (MQ=255)
                                                                                                                                    aaaaTAATCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAgg            >  1:49351/1‑148 (MQ=255)
                                                                                                                                       ataatCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGctc         <  2:2854803/148‑1 (MQ=255)
                                                                                                                                       ataatCCCTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGctc         <  1:805347/148‑1 (MQ=255)
                                                                                                                                            cccTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTAtcg    >  1:1825823/1‑148 (MQ=255)
                                                                                                                                            cccTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTAtcg    >  1:2787841/1‑148 (MQ=255)
                                                                                                                                            cccTGCCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTAtcg    >  2:696547/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GCGAAGGAAAACTCACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTATTGGAATTATTTTCGCCAGGCTCTATCGTC  >  CP009273/3186036‑3186325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑