Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,189,569 C→A 100% D182Y (GAT→TAT)  hldE ← fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,189,5690CA96.2% 531.1 / 9.7 184D182Y (GAT→TAT) hldEfused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
Reads supporting (aligned to +/- strand):  ref base C (5/3);  new base A (100/76);  total (105/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 184 total aligned reads displayed.

CACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTG  >  CP009273/3189423‑3189713
                                                                                                                                                  |                                                                                                                                                
   tAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTa                                                                                                                                              <  1:389748/148‑1 (MQ=255)
    aaCAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTAc                                                                                                                                             <  1:920382/148‑1 (MQ=255)
     aCAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTAcc                                                                                                                                            <  1:2054933/148‑1 (MQ=255)
     aCAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTAcc                                                                                                                                            <  1:1816633/148‑1 (MQ=255)
     aCAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTAcc                                                                                                                                            <  1:1702349/148‑1 (MQ=255)
      cAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCt                                                                                                                                           <  1:1410682/148‑1 (MQ=255)
            cGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGa                                                                                                                                     >  1:1805418/1‑148 (MQ=255)
             gagaGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGat                                                                                                                                    <  2:839982/148‑1 (MQ=255)
               gagTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatca                                                                                                                                  >  1:3302650/1‑148 (MQ=255)
               gagTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatca                                                                                                                                  >  2:1075501/1‑148 (MQ=255)
                 tttCGTAATCGGCAATCATTTTCATGCCGCGCTCAACATTCTCTTATTCGGTCTTACATTTACAGACATCAGCTTCAAATTCCGAGAGATTCTTCGTTAACAGCGTAGCGCCGCGGTTGCTCTCTAAATAGGTACCTTTTGGatcaat                                                                                                                                <  2:2833527/147‑1 (MQ=255)
                 gTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaat                                                                                                                                <  2:2139674/148‑1 (MQ=255)
                 gTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaat                                                                                                                                <  1:1386754/148‑1 (MQ=255)
                  ttCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaatc                                                                                                                               >  1:2627376/1‑148 (MQ=255)
                   tCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGatcaatca                                                                                                                              >  1:1928644/1‑148 (MQ=255)
                   tCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaatca                                                                                                                              >  1:2117352/1‑148 (MQ=255)
                   tCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaatca                                                                                                                              >  1:2117475/1‑148 (MQ=255)
                   tCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGatcaatca                                                                                                                              >  1:536875/1‑148 (MQ=255)
                             cAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAac                                                                                                                    <  1:1622964/148‑1 (MQ=255)
                             cAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAac                                                                                                                    >  2:744634/1‑148 (MQ=255)
                              aaTCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAaca                                                                                                                   <  2:1572995/148‑1 (MQ=255)
                                tCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACAcc                                                                                                                 >  2:555775/1‑148 (MQ=255)
                                    tttCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCt                                                                                                             >  2:508374/1‑148 (MQ=255)
                                    tttCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCt                                                                                                             <  2:1129521/148‑1 (MQ=255)
                                    tttCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTGACCGACAACAGCTTCAAATGCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCt                                                                                                             <  2:2303010/148‑1 (MQ=255)
                                    tttAATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGAGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAAACAGCACAGGAACACCCGCt                                                                                                             >  2:3412702/1‑148 (MQ=255)
                                      tCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCttt                                                                                                           <  1:2465344/148‑1 (MQ=255)
                                       cATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTa                                                                                                          <  1:1064440/148‑1 (MQ=255)
                                       cATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTa                                                                                                          <  1:1064438/148‑1 (MQ=255)
                                         tGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCACAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTAcg                                                                                                        >  1:3001741/1‑148 (MQ=255)
                                           ccGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTAcgcg                                                                                                      <  2:960315/148‑1 (MQ=255)
                                            cgcgCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACgcgc                                                                                                     <  1:3403791/148‑1 (MQ=255)
                                               gcTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAg                                                                                                  <  2:1183382/148‑1 (MQ=255)
                                               gcTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTGTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAg                                                                                                  <  2:3351899/148‑1 (MQ=255)
                                                  caacaaTCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTg                                                                                               >  1:862753/1‑148 (MQ=255)
                                                   aacaaTCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTgg                                                                                              >  2:1316614/1‑148 (MQ=255)
                                                    acaaTCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGa                                                                                             <  1:3141701/148‑1 (MQ=255)
                                                     caaTCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGat                                                                                            >  1:1184120/1‑148 (MQ=255)
                                                     caaTCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGat                                                                                            <  2:1958262/148‑1 (MQ=255)
                                                     caaTCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGat                                                                                            >  1:3477270/1‑148 (MQ=255)
                                                     caaTCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGat                                                                                            >  2:2476823/1‑148 (MQ=255)
                                                      aaTCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTGTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatc                                                                                           <  2:2595420/148‑1 (MQ=255)
                                                        tctcTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcat                                                                                         >  1:2854731/1‑148 (MQ=255)
                                                        tctcGTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcat                                                                                         <  2:1911083/148‑1 (MQ=255)
                                                         ctctTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCTTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcatc                                                                                        >  1:2245977/1‑148 (MQ=255)
                                                         ctctTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTCGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGagcatc                                                                                        >  2:1724101/1‑148 (MQ=255)
                                                         ctctTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcatc                                                                                        >  1:479730/1‑148 (MQ=255)
                                                         ctctTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGatcatc                                                                                        >  1:3188465/1‑148 (MQ=255)
                                                           cttcttCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATctg                                                                                      >  2:2228875/1‑148 (MQ=255)
                                                           cttcttCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATctg                                                                                      >  1:187473/1‑148 (MQ=255)
                                                              cttcGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATctgctg                                                                                   <  2:2605538/148‑1 (MQ=255)
                                                              cttcGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATctgctg                                                                                   <  2:2605441/148‑1 (MQ=255)
                                                                tcGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTa                                                                                 <  1:791790/148‑1 (MQ=255)
                                                                   gTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGc                                                                              <  1:1446550/148‑1 (MQ=255)
                                                                      ttACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTg                                                                           >  2:533929/1‑148 (MQ=255)
                                                                      ttACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGAGTTAACAGCGTAGAGCCGCTGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGGTGGATCATCTGCTGTACGCTTg                                                                           >  2:533995/1‑148 (MQ=255)
                                                                               ccGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCAc                                                                  >  2:2786006/1‑148 (MQ=255)
                                                                               ccGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCAc                                                                  >  2:2017918/1‑148 (MQ=255)
                                                                                cGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCAcc                                                                 >  1:128495/1‑148 (MQ=255)
                                                                                    aacaGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTg                                                             <  1:3197936/148‑1 (MQ=255)
                                                                                    aacaGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTg                                                             >  2:343077/1‑148 (MQ=255)
                                                                                    aacaGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTg                                                             >  2:1586280/1‑148 (MQ=255)
                                                                                    aacaGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTg                                                             <  2:2456292/148‑1 (MQ=255)
                                                                                          ttCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAg                                                       <  2:2408441/148‑1 (MQ=255)
                                                                                           tCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGt                                                      >  2:234172/1‑148 (MQ=255)
                                                                                            cAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGGCAGTTGGATCATCTGCTGTACGCTTGCCCGCGCCCCGTTGGCggcgtg                                                     >  1:1812025/1‑143 (MQ=255)
                                                                                             aaaTTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCa                                                    >  1:1548307/1‑148 (MQ=255)
                                                                                               aTTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGa                                                  >  2:2960444/1‑148 (MQ=255)
                                                                                               aTTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGa                                                  <  2:2669140/148‑1 (MQ=255)
                                                                                                ttCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGaa                                                 <  1:2328666/148‑1 (MQ=255)
                                                                                                 tCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGaaa                                                >  1:2148716/1‑148 (MQ=255)
                                                                                                 tCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGaaa                                                >  1:3389218/1‑148 (MQ=255)
                                                                                                  ccGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAg                                               >  1:667948/1‑148 (MQ=255)
                                                                                                  ccGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAg                                               >  1:2430724/1‑148 (MQ=255)
                                                                                                    gagagaTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCa                                             <  2:2368152/148‑1 (MQ=255)
                                                                                                       agaTTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCa                                          >  2:3486644/1‑148 (MQ=255)
                                                                                                          ttCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAgcg                                       <  2:1928644/148‑1 (MQ=255)
                                                                                                             ggCTTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTGTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAgcgcgc                                    <  2:3468111/148‑1 (MQ=255)
                                                                                                              gCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGcc                                   >  2:1805441/1‑148 (MQ=255)
                                                                                                              gCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGcc                                   >  2:507762/1‑148 (MQ=255)
                                                                                                               cGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCa                                  >  2:1461525/1‑148 (MQ=255)
                                                                                                               cGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCa                                  <  1:1426104/148‑1 (MQ=255)
                                                                                                               cGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCa                                  >  2:42834/1‑148 (MQ=255)
                                                                                                                  tAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGTGTAGTCAGAAAGCACCAGCGCGCCACTc                               >  2:1855491/1‑148 (MQ=255)
                                                                                                                  tAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATc                               >  1:2235504/1‑148 (MQ=255)
                                                                                                                  tAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATc                               >  2:406509/1‑148 (MQ=255)
                                                                                                                  tAACAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATc                               >  2:1912180/1‑148 (MQ=255)
                                                                                                                    aCAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGa                             >  1:971565/1‑148 (MQ=255)
                                                                                                                     cAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGaa                            <  1:2173160/148‑1 (MQ=255)
                                                                                                                     cAGCGTAGCGCCGCGGTAGCGCTCAAAATAGGTACCTGTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGaa                            <  1:2454571/148‑1 (MQ=255)
                                                                                                                                     tAGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTaa            <  1:2880063/148‑1 (MQ=255)
                                                                                                                                     tAGAGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTaa            <  1:2814208/148‑1 (MQ=255)
                                                                                                                                      aGCGCTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAAt           <  1:1425736/148‑1 (MQ=255)
                                                                                                                                          cTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGc       >  2:2897609/1‑148 (MQ=255)
                                                                                                                                          cTCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGc       >  2:2897557/1‑148 (MQ=255)
                                                                                                                                           tCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCt      >  2:727008/1‑148 (MQ=255)
                                                                                                                                           tCAAAATAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCt      >  2:2487945/1‑148 (MQ=255)
                                                                                                                                              aaaTAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGt   <  1:555775/148‑1 (MQ=255)
                                                                                                                                              aaaTAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGt   >  2:1615273/1‑148 (MQ=255)
                                                                                                                                               aaTAGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTg  <  2:2851072/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CACTAACAGAGCCGAGAGTTCGTAATCGGCAATCAGTTTCATGCCGCGCTCAACAATCTCTTCTTCGGTCTTACATTTACCGACAACAGCTTCAAATTCCGAGAGATTCGGCGTTAACAGCGTAGCGCCGCGGTAGCGCTCAAAATCGGTACCTTTTGGATCAATCAGCACCGGAACACCCGCTTTACGCGCCAGTTGGATCATCTGCTGTACGCTTGCCAGCGCACCTTTGGCGTAGTCAGAAAGCACCAGCGCGCCAATCGAACTCAGCGCCTGATTAATCCGCTCGTG  >  CP009273/3189423‑3189713

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑