Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,212,264 G→T 70.4% H58N (CAC→AAC)  aer ← fused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,212,2640GT70.4% 309.0 / 268.8 277H58N (CAC→AAC) aerfused signal transducer for aerotaxis sensory component/methyl accepting chemotaxis component
Reads supporting (aligned to +/- strand):  ref base G (33/49);  new base T (102/93);  total (135/142)
Fisher's exact test for biased strand distribution p-value = 8.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 277 total aligned reads displayed.

CCTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGC  >  CP009273/3212120‑3212409
                                                                                                                                                |                                                                                                                                                 
  tCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCa                                                                                                                                              >  2:448697/1‑148 (MQ=255)
     cgcACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCa                                                                                                                                           <  1:1740173/148‑1 (MQ=255)
       cACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCAtg                                                                                                                                         <  2:2536142/148‑1 (MQ=255)
        aCCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCAtgt                                                                                                                                        >  2:2918060/1‑148 (MQ=255)
        aCCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCAtgt                                                                                                                                        >  2:954463/1‑148 (MQ=255)
          cATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCAtgttg                                                                                                                                      >  2:1185201/1‑148 (MQ=255)
             cGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGc                                                                                                                                   >  1:463124/1‑148 (MQ=255)
             cGGTACCGCATGGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGc                                                                                                                                   <  2:603748/148‑1 (MQ=255)
               gTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCgg                                                                                                                                 <  2:346238/148‑1 (MQ=255)
               gTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCgg                                                                                                                                 <  2:1359831/148‑1 (MQ=255)
               gTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCgg                                                                                                                                 <  2:342248/148‑1 (MQ=255)
                tACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGc                                                                                                                                <  1:633122/148‑1 (MQ=255)
                 aCCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCt                                                                                                                               >  1:703934/1‑148 (MQ=255)
                 aCCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCt                                                                                                                               <  1:1954088/148‑1 (MQ=255)
                     cATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGccc                                                                                                                           >  2:2745335/1‑148 (MQ=255)
                     cATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGccc                                                                                                                           <  1:2255800/148‑1 (MQ=255)
                      aTTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCCGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCt                                                                                                                          <  1:2441339/148‑1 (MQ=255)
                          gCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTa                                                                                                                      <  2:229025/148‑1 (MQ=255)
                          gCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTa                                                                                                                      >  1:490928/1‑148 (MQ=255)
                           cccGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTaa                                                                                                                     <  1:1732129/148‑1 (MQ=255)
                             cGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAAct                                                                                                                   <  1:118288/148‑1 (MQ=255)
                             cGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAAct                                                                                                                   >  2:2689123/1‑148 (MQ=255)
                                     ataatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAg                                                                                                           >  2:1939530/1‑148 (MQ=255)
                                     ataatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAg                                                                                                           <  2:3353746/148‑1 (MQ=255)
                                      taatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAgg                                                                                                          >  2:8309/1‑148 (MQ=255)
                                       aatGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGt                                                                                                         >  2:3135105/1‑148 (MQ=255)
                                        atGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGta                                                                                                        <  1:2137257/148‑1 (MQ=255)
                                        atGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGta                                                                                                        <  1:1699538/148‑1 (MQ=255)
                                        atGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGta                                                                                                        <  1:371453/148‑1 (MQ=255)
                                         tGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGtat                                                                                                       >  1:2524634/1‑148 (MQ=255)
                                          ggTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGtata                                                                                                      >  2:1397188/1‑148 (MQ=255)
                                           gTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACAAATCCGCAAACGCCGCTTTTGGCATATCAGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGAAAGGTATAg                                                                                                     >  1:2884831/1‑148 (MQ=255)
                                             cACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGcc                                                                                                   >  1:1017926/1‑148 (MQ=255)
                                              aCCATTTTTGCGGCTATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCg                                                                                                  <  2:2121815/148‑1 (MQ=255)
                                                   ttttGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCAc                                                                                             >  2:743574/1‑148 (MQ=255)
                                                    tttGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCAcc                                                                                            <  2:31983/148‑1 (MQ=255)
                                                          gcgATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGGTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTCTAGCCGCTCACCTGcaca                                                                                      >  2:637560/1‑148 (MQ=255)
                                                            gATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACaaa                                                                                    <  1:1243488/148‑1 (MQ=255)
                                                               ttttCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAgt                                                                                 >  2:2237923/1‑148 (MQ=255)
                                                               ttttCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAgt                                                                                 <  1:2646780/148‑1 (MQ=255)
                                                                tttCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAgtg                                                                                <  1:1848373/148‑1 (MQ=255)
                                                                   cACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCa                                                                             <  1:762828/148‑1 (MQ=255)
                                                                    aCGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCAt                                                                            >  2:1120756/1‑148 (MQ=255)
                                                                    aCGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCAt                                                                            <  1:1278193/148‑1 (MQ=255)
                                                                    aCGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCAt                                                                            <  1:2326108/148‑1 (MQ=255)
                                                                     cGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCAtt                                                                           >  1:1564993/1‑148 (MQ=255)
                                                                      gATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCACACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTa                                                                          <  1:1982794/148‑1 (MQ=255)
                                                                       aTGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAg                                                                         <  1:1188772/148‑1 (MQ=255)
                                                                        tGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGc                                                                        >  2:581527/1‑148 (MQ=255)
                                                                        tGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGc                                                                        >  2:739863/1‑148 (MQ=255)
                                                                         gCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCa                                                                       <  1:2135399/148‑1 (MQ=255)
                                                                         gCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCa                                                                       <  2:1437927/148‑1 (MQ=255)
                                                                          ccGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCAt                                                                      >  1:1830338/1‑148 (MQ=255)
                                                                           cGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATg                                                                     >  2:822264/1‑148 (MQ=255)
                                                                           cGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATACGTGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATg                                                                     <  1:1008160/148‑1 (MQ=255)
                                                                            gCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGa                                                                    <  1:3135124/148‑1 (MQ=255)
                                                                             cTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAg                                                                   <  2:1880814/148‑1 (MQ=255)
                                                                              tCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGt                                                                  <  1:3408274/148‑1 (MQ=255)
                                                                               ccAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGTCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTg                                                                 >  1:2782691/1‑148 (MQ=255)
                                                                                 aGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGat                                                               >  1:84165/1‑148 (MQ=255)
                                                                                 aGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGat                                                               <  2:1985336/148‑1 (MQ=255)
                                                                                  gggCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGata                                                              >  2:234320/1‑148 (MQ=255)
                                                                                  gggCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGata                                                              <  2:3329022/148‑1 (MQ=255)
                                                                                   ggCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCAACAGTGagat                                                             >  2:2058514/1‑148 (MQ=255)
                                                                                    gCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGAtata                                                            <  1:3103623/148‑1 (MQ=255)
                                                                                    gCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAAAGCCGCTTATGGCATATACGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGGAAGGTATAGCCGCTCTCCAGCACAATAGTGTCATTTGCCTGAGTGAgata                                                            >  2:2548943/1‑148 (MQ=255)
                                                                                     cTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAg                                                           <  1:3293738/148‑1 (MQ=255)
                                                                                       ccccTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCt                                                         >  1:2919811/1‑148 (MQ=255)
                                                                                       ccccTTTTTTCAGGGTGAACCACAGAGCCGCACACGCCGCTTTTGGCATATCCGGGTGACGCACCAGGTTGTGCGGCTGCCCTTGTACCTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCt                                                         <  1:2813241/148‑1 (MQ=255)
                                                                                        cccTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCtt                                                        <  2:2658611/148‑1 (MQ=255)
                                                                                        cccTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCtt                                                        <  2:2824288/148‑1 (MQ=255)
                                                                                           ttttttCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGc                                                     >  1:879451/1‑148 (MQ=255)
                                                                                             ttttCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAg                                                   >  2:1472853/1‑148 (MQ=255)
                                                                                                 cAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCg                                               <  1:297597/148‑1 (MQ=255)
                                                                                                   gggTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGt                                             >  1:2859547/1‑148 (MQ=255)
                                                                                                   gggTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGt                                             >  1:1109215/1‑148 (MQ=255)
                                                                                                    ggTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTa                                            >  1:1714314/1‑148 (MQ=255)
                                                                                                         aCCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGa                                       <  1:2920989/148‑1 (MQ=255)
                                                                                                          ccACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGAc                                      >  2:64160/1‑148 (MQ=255)
                                                                                                          ccACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGAc                                      >  1:2775124/1‑148 (MQ=255)
                                                                                                           cacaTATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACa                                     <  2:1710025/148‑1 (MQ=255)
                                                                                                             caTATCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATc                                   >  1:1553255/1‑148 (MQ=255)
                                                                                                               tatCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCag                                 >  1:732028/1‑148 (MQ=255)
                                                                                                                atCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCaga                                <  1:1987593/148‑1 (MQ=255)
                                                                                                                atCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCaga                                >  1:2207341/1‑148 (MQ=255)
                                                                                                                atCCGCAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCaga                                >  2:2403860/1‑148 (MQ=255)
                                                                                                                   cGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTg                             <  2:484734/148‑1 (MQ=255)
                                                                                                                    gCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTgg                            <  2:28410/148‑1 (MQ=255)
                                                                                                                     cAAACGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGt                           >  1:1456429/1‑148 (MQ=255)
                                                                                                                       aaCGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTAt                         <  1:954463/148‑1 (MQ=255)
                                                                                                                        aCGCCGCTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATc                        <  2:1017926/148‑1 (MQ=255)
                                                                                                                              cTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGc                  >  2:2801201/1‑148 (MQ=255)
                                                                                                                              cTTTTGGCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGc                  <  1:1361944/148‑1 (MQ=255)
                                                                                                                                   ggCATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCg             <  2:1571603/148‑1 (MQ=255)
                                                                                                                                    gCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCgg            >  1:531019/1‑148 (MQ=255)
                                                                                                                                     cATATCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCggg           >  2:1782105/1‑148 (MQ=255)
                                                                                                                                         tCCGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATAGTCCGCCAGCGGGGTAt       <  1:2689142/148‑1 (MQ=255)
                                                                                                                                          ccGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAtt      <  1:2613775/148‑1 (MQ=255)
                                                                                                                                          ccGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAtt      >  2:685890/1‑148 (MQ=255)
                                                                                                                                           cGGGTTACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTAttt     <  2:1523100/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
CCTCGCGCACCATCGGTACCGCATTGGCCCGCACCCAATAATGGTCACCATTTTTGCGGCGATTTTTCACGATGCCGCTCCAGGGCTCCCCTTTTTTCAGGGTGAACCACATATCCGCAAACGCCGCTTTTGGCATATCCGGGTGACGCACCATGTTGTGCGGCTGCCCTTGTAACTCTTGCAAGGTATAGCCGCTCACCTGCACAAAAGTGTCATTAGCATGAGTGATATAGCTTTGCAGATCGGTAGTGGACATCAGAGTGGTATCGTCCGCCAGCGGGGTATTTTGC  >  CP009273/3212120‑3212409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑