Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,261,001 G→A 100% intergenic (+44/‑212) tdcR → / → yhaB L‑threonine dehydratase operon activator protein/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,261,0010GA99.6% 828.3 / ‑4.5 248intergenic (+44/‑212)tdcR/yhaBL‑threonine dehydratase operon activator protein/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (113/134);  total (114/134)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 248 total aligned reads displayed.

AAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATA  >  CP009273/3260856‑3261144
                                                                                                                                                 |                                                                                                                                               
  aaTTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATca                                                                                                                                             >  1:3315662/1‑148 (MQ=255)
    tttCGAATCCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATcaca                                                                                                                                           <  2:1904685/148‑1 (MQ=255)
     ttCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACaa                                                                                                                                          <  2:2259774/148‑1 (MQ=255)
     ttCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACaa                                                                                                                                          >  2:1266878/1‑148 (MQ=255)
       cGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACaaaa                                                                                                                                        <  1:2175486/148‑1 (MQ=255)
           tACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaa                                                                                                                                    <  1:2467236/148‑1 (MQ=255)
           tACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaa                                                                                                                                    <  1:704175/148‑1 (MQ=255)
            aCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaat                                                                                                                                   <  1:132741/148‑1 (MQ=255)
            aCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaat                                                                                                                                   >  2:2343101/1‑148 (MQ=255)
            aCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaat                                                                                                                                   <  1:2233322/148‑1 (MQ=255)
             cATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaata                                                                                                                                  >  1:1416982/1‑148 (MQ=255)
              aTGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaataa                                                                                                                                 >  2:2131647/1‑148 (MQ=255)
              aTGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaataa                                                                                                                                 >  2:560231/1‑148 (MQ=255)
              aTGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaataa                                                                                                                                 <  2:395066/148‑1 (MQ=255)
                gTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAAtt                                                                                                                               <  1:2016470/148‑1 (MQ=255)
                  aTTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGc                                                                                                                             >  1:760404/1‑148 (MQ=255)
                  aTTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGc                                                                                                                             >  2:2567942/1‑148 (MQ=255)
                    tGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTg                                                                                                                           <  1:1518326/148‑1 (MQ=255)
                     gATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGa                                                                                                                          <  1:1607016/148‑1 (MQ=255)
                      atcatcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGAt                                                                                                                         <  2:1465072/148‑1 (MQ=255)
                      atcatcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGAt                                                                                                                         <  2:1642304/148‑1 (MQ=255)
                                aCAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATa                                                                                                               <  2:2528435/148‑1 (MQ=255)
                                aCAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATa                                                                                                               <  2:1043903/148‑1 (MQ=255)
                                 cAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATaa                                                                                                              <  2:204620/148‑1 (MQ=255)
                                  aaTTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAg                                                                                                             >  2:34718/1‑148 (MQ=255)
                                   aTTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAgg                                                                                                            >  2:2759346/1‑148 (MQ=255)
                                     tGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATCATTGCTGATCGCCGCGATAAGGTc                                                                                                          >  2:3043366/1‑148 (MQ=255)
                                     tGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTc                                                                                                          <  2:3187379/148‑1 (MQ=255)
                                     tGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTc                                                                                                          >  2:2392297/1‑148 (MQ=255)
                                       aTTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCag                                                                                                        >  1:667102/1‑148 (MQ=255)
                                       aTTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCag                                                                                                        <  1:2770182/148‑1 (MQ=255)
                                       aTTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCag                                                                                                        <  1:642054/148‑1 (MQ=255)
                                           aCGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaa                                                                                                    >  2:641849/1‑148 (MQ=255)
                                            cGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaa                                                                                                   >  2:2602722/1‑148 (MQ=255)
                                            cGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaa                                                                                                   >  1:497253/1‑148 (MQ=255)
                                              tCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaaga                                                                                                 >  1:3109879/1‑148 (MQ=255)
                                                aaCTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaagaca                                                                                               <  2:3375470/148‑1 (MQ=255)
                                                  cTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGacaac                                                                                             >  1:2613468/1‑148 (MQ=255)
                                                   tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGacaaca                                                                                            <  2:298888/148‑1 (MQ=255)
                                                   tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGacaaca                                                                                            <  2:3280506/148‑1 (MQ=255)
                                                    ttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGAcaacaa                                                                                           <  2:3004222/148‑1 (MQ=255)
                                                     tttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAg                                                                                          <  1:862211/148‑1 (MQ=255)
                                                        ctctTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGa                                                                                       >  1:2041491/1‑148 (MQ=255)
                                                         tcttctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGaa                                                                                      <  1:1686834/148‑1 (MQ=255)
                                                          cttctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGaaa                                                                                     <  1:1982358/148‑1 (MQ=255)
                                                                 cAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTcac                                                                              >  2:1406793/1‑148 (MQ=255)
                                                                 cAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTcac                                                                              >  2:26718/1‑148 (MQ=255)
                                                                 cAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTcac                                                                              <  2:2736391/148‑1 (MQ=255)
                                                                    gACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACaga                                                                           >  1:312324/1‑148 (MQ=255)
                                                                    gACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCAAAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACaga                                                                           >  1:312595/1‑148 (MQ=255)
                                                                     aCGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACagag                                                                          <  1:1889012/148‑1 (MQ=255)
                                                                     aCGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACagag                                                                          >  2:1275033/1‑148 (MQ=255)
                                                                        tCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCtt                                                                       <  2:1000067/148‑1 (MQ=255)
                                                                        tCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCtt                                                                       <  2:1593710/148‑1 (MQ=255)
                                                                         cATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCttt                                                                      <  1:1009976/148‑1 (MQ=255)
                                                                            tttGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGa                                                                   >  1:2687480/1‑148 (MQ=255)
                                                                              tgtgAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATc                                                                 <  1:1887955/148‑1 (MQ=255)
                                                                                 gAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGc                                                              >  1:1741961/1‑148 (MQ=255)
                                                                                  aaTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCg                                                             <  1:2126473/148‑1 (MQ=255)
                                                                                   aTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGt                                                            <  2:961483/148‑1 (MQ=255)
                                                                                   aTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGt                                                            <  2:3423673/148‑1 (MQ=255)
                                                                                   aTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGt                                                            <  1:117411/148‑1 (MQ=255)
                                                                                   aTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGt                                                            <  1:1425144/148‑1 (MQ=255)
                                                                                             tttCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGa                                                  <  2:667102/148‑1 (MQ=255)
                                                                                                   tAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTg                                            <  1:2384985/148‑1 (MQ=255)
                                                                                                    aaaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGc                                           <  2:1218905/148‑1 (MQ=255)
                                                                                                    aaaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGc                                           <  1:1800683/148‑1 (MQ=255)
                                                                                                     aaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCt                                          <  1:1064409/148‑1 (MQ=255)
                                                                                                     aaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCt                                          >  1:2163011/1‑148 (MQ=255)
                                                                                                     aaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCt                                          <  1:2343101/148‑1 (MQ=255)
                                                                                                     aaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCt                                          >  1:3055215/1‑148 (MQ=255)
                                                                                                      aTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCtt                                         <  1:344756/148‑1 (MQ=255)
                                                                                                        tcttctCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCg                                       <  2:407442/148‑1 (MQ=255)
                                                                                                             tcCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTAc                                  <  2:1033763/148‑1 (MQ=255)
                                                                                                              ccccTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATATTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACt                                 >  1:1212389/1‑148 (MQ=255)
                                                                                                               cccTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTg                                <  2:2451015/148‑1 (MQ=255)
                                                                                                               cccTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTg                                >  1:193689/1‑148 (MQ=255)
                                                                                                                ccTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGt                               <  1:2325260/148‑1 (MQ=255)
                                                                                                                 cTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTa                              >  2:2853198/1‑148 (MQ=255)
                                                                                                                   cATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAAc                            >  1:37592/1‑148 (MQ=255)
                                                                                                                   cATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAAc                            >  2:2800444/1‑148 (MQ=255)
                                                                                                                    aTAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACg                           <  2:1822945/148‑1 (MQ=255)
                                                                                                                       ggCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCg                        <  1:1267136/148‑1 (MQ=255)
                                                                                                                        gCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCgg                       >  2:480749/1‑148 (MQ=255)
                                                                                                                         cgacgaATAGCTTTTTGTTTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCggg                      <  1:1816916/148‑1 (MQ=255)
                                                                                                                         cgacgaATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCggg                      <  1:1915896/148‑1 (MQ=255)
                                                                                                                          gacgaATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGt                     >  1:572059/1‑148 (MQ=255)
                                                                                                                            cgaATAGCATTTTGTGTTGAGAATCACAAAACTAATAATTGCTTATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGATTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTaa                   <  1:956223/148‑1 (MQ=255)
                                                                                                                            cgaATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTaa                   <  2:1729220/148‑1 (MQ=255)
                                                                                                                            cgaATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTaa                   <  1:3248430/148‑1 (MQ=255)
                                                                                                                               aTAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATg                >  2:236048/1‑148 (MQ=255)
                                                                                                                                tAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGa               <  1:2574440/148‑1 (MQ=255)
                                                                                                                                tAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGa               >  2:19005/1‑148 (MQ=255)
                                                                                                                                 aGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGGAAATGAg              >  2:2556856/1‑148 (MQ=255)
                                                                                                                                  gCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGc             >  2:812375/1‑148 (MQ=255)
                                                                                                                                   cATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCg            <  1:1260393/148‑1 (MQ=255)
                                                                                                                                    aTTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGt           >  1:304687/1‑148 (MQ=255)
                                                                                                                                    aTTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACGAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGt           <  2:2031009/148‑1 (MQ=255)
                                                                                                                                    aTTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGt           <  2:115840/148‑1 (MQ=255)
                                                                                                                                             tGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATa  >  1:3190722/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
AAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATA  >  CP009273/3260856‑3261144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑