Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,290,970 A→T 100% R176R (CGT→CGA)  yraQ ← putative inner membrane permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,290,9700AT99.4% 496.2 / ‑4.4 152R176R (CGT→CGA) yraQputative inner membrane permease
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (87/64);  total (87/65)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 152 total aligned reads displayed.

ATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGC  >  CP009273/3290825‑3291113
                                                                                                                                                 |                                                                                                                                               
aTAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCg                                                                                                                                               >  2:1892945/1‑148 (MQ=255)
  aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGaa                                                                                                                                             >  2:2386296/1‑148 (MQ=255)
  aGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGaa                                                                                                                                             <  1:3395760/148‑1 (MQ=255)
       ccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATcgcc                                                                                                                                        >  1:1393377/1‑148 (MQ=255)
       ccTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATcgcc                                                                                                                                        >  1:2186974/1‑148 (MQ=255)
              cAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAc                                                                                                                                 >  2:3363385/1‑148 (MQ=255)
              cAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAc                                                                                                                                 >  1:3324703/1‑148 (MQ=255)
              cAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAc                                                                                                                                 >  1:1886273/1‑148 (MQ=255)
              cAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAc                                                                                                                                 >  1:1886201/1‑148 (MQ=255)
              cAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAAc                                                                                                                                 >  1:1585586/1‑148 (MQ=255)
                      aaaaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCg                                                                                                                         >  2:2168005/1‑148 (MQ=255)
                        aaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAg                                                                                                                       >  1:3227796/1‑148 (MQ=255)
                        aaaaCCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAg                                                                                                                       >  1:3227881/1‑148 (MQ=255)
                             ccGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACaa                                                                                                                  <  1:1418866/148‑1 (MQ=255)
                              cGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACaaa                                                                                                                 >  2:2963397/1‑148 (MQ=255)
                               gCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAg                                                                                                                <  2:803654/148‑1 (MQ=255)
                               gCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAg                                                                                                                >  1:2447894/1‑148 (MQ=255)
                                     tgCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAgg                                                                                                                      >  1:2146468/1‑136 (MQ=255)
                                      gCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATaa                                                                                                         <  2:421549/148‑1 (MQ=255)
                                        ttCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAac                                                                                                       <  1:1300150/148‑1 (MQ=255)
                                            ggTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCa                                                                                                   >  1:642381/1‑148 (MQ=255)
                                             gTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAg                                                                                                  <  1:1004/148‑1 (MQ=255)
                                              tATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGc                                                                                                 >  1:1518899/1‑148 (MQ=255)
                                               aTGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCg                                                                                                <  2:1425281/148‑1 (MQ=255)
                                               aTGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCg                                                                                                >  1:1084148/1‑148 (MQ=255)
                                                tGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGt                                                                                               <  1:117294/148‑1 (MQ=255)
                                                tGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGt                                                                                               >  2:2066195/1‑148 (MQ=255)
                                                 gTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTc                                                                                              >  2:124128/1‑148 (MQ=255)
                                                   cAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGGGTCGc                                                                                            <  2:1414557/148‑1 (MQ=255)
                                                   cAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGc                                                                                            <  2:3160398/148‑1 (MQ=255)
                                                   cAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGc                                                                                            >  2:404967/1‑148 (MQ=255)
                                                     aTTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCg                                                                                          <  2:2621968/148‑1 (MQ=255)
                                                     aTTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCg                                                                                          <  1:382033/148‑1 (MQ=255)
                                                     aTTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCg                                                                                          >  2:46259/1‑148 (MQ=255)
                                                     aTTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCg                                                                                          >  1:1951197/1‑148 (MQ=255)
                                                      tttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCCTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCgg                                                                                         >  1:775195/1‑148 (MQ=255)
                                                       ttCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCggg                                                                                        >  1:63693/1‑148 (MQ=255)
                                                           aCCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTa                                                                                    <  2:816147/148‑1 (MQ=255)
                                                            ccGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCCGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACACAGCCCATAAACACCAGCGTCGCCGGGTGTaa                                                                                   >  2:3340602/1‑148 (MQ=255)
                                                            ccGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTaa                                                                                   >  2:2435459/1‑148 (MQ=255)
                                                             cGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAac                                                                                  <  2:676601/148‑1 (MQ=255)
                                                             cGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAac                                                                                  <  2:2437844/148‑1 (MQ=255)
                                                              ggTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAaca                                                                                 <  1:1799301/148‑1 (MQ=255)
                                                                   cTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGa                                                                            <  1:1385372/148‑1 (MQ=255)
                                                                   cTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGa                                                                            >  1:2811552/1‑148 (MQ=255)
                                                                   cTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGa                                                                            <  2:1627890/148‑1 (MQ=255)
                                                                   cTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGa                                                                            <  1:692678/148‑1 (MQ=255)
                                                                    tGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGAt                                                                           >  1:1750914/1‑148 (MQ=255)
                                                                         ttGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGccc                                                                      >  2:2653868/1‑148 (MQ=255)
                                                                          tGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCa                                                                     <  2:225488/148‑1 (MQ=255)
                                                                              gtgtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATcct                                                                 >  2:3465587/1‑147 (MQ=255)
                                                                               tgtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAg                                                                >  1:3159194/1‑148 (MQ=255)
                                                                                gtTTCACGCGCCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGa                                                               >  2:2091074/1‑148 (MQ=255)
                                                                                gtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGa                                                               <  2:2792277/148‑1 (MQ=255)
                                                                                gtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGa                                                               <  1:1462239/148‑1 (MQ=255)
                                                                                gtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGa                                                               <  2:1039936/148‑1 (MQ=255)
                                                                                gtTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGa                                                               >  2:1929411/1‑148 (MQ=255)
                                                                                gtTTCACGCACCCAGTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGa                                                               <  2:2782050/148‑1 (MQ=255)
                                                                                 tttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                              >  2:534872/1‑148 (MQ=255)
                                                                                 tttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                              >  2:798821/1‑148 (MQ=255)
                                                                                 tttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                              <  1:244408/148‑1 (MQ=255)
                                                                                 tttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                              <  1:244409/148‑1 (MQ=255)
                                                                                 tttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                              <  2:867958/148‑1 (MQ=255)
                                                                                 tttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                              >  1:338444/1‑148 (MQ=255)
                                                                                 tttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGaa                                                              >  1:338413/1‑148 (MQ=255)
                                                                                  ttCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAAt                                                             >  1:2454014/1‑148 (MQ=255)
                                                                                    cACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGc                                                           <  1:1042085/148‑1 (MQ=255)
                                                                                    cACGCACCCATTTGTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGc                                                           <  2:670942/148‑1 (MQ=255)
                                                                                      cGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCa                                                         <  2:1309168/148‑1 (MQ=255)
                                                                                         aCCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAgcg                                                      >  2:1605605/1‑148 (MQ=255)
                                                                                             aTTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCAcc                                                  >  2:13611/1‑148 (MQ=255)
                                                                                               ttttGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGc                                                >  1:2366320/1‑148 (MQ=255)
                                                                                                 ttGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGccc                                              >  2:1585560/1‑148 (MQ=255)
                                                                                                  tGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCa                                             <  2:184727/148‑1 (MQ=255)
                                                                                                   gCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCAt                                            <  2:2845709/148‑1 (MQ=255)
                                                                                                    cACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATc                                           >  2:79946/1‑148 (MQ=255)
                                                                                                    cACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATc                                           >  1:3122959/1‑148 (MQ=255)
                                                                                                        aGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGaca                                       >  1:3253283/1‑148 (MQ=255)
                                                                                                        aGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGaca                                       <  2:400581/148‑1 (MQ=255)
                                                                                                          cGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACAcc                                     >  2:1222819/1‑148 (MQ=255)
                                                                                                            tCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTg                                   <  2:392826/148‑1 (MQ=255)
                                                                                                             cGCAATCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGt                                  <  1:2193213/148‑1 (MQ=255)
                                                                                                                 aTCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGc                              >  1:625685/1‑148 (MQ=255)
                                                                                                                  tCAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCg                             >  1:1214485/1‑148 (MQ=255)
                                                                                                                   cAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGa                            <  2:3324703/148‑1 (MQ=255)
                                                                                                                   cAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGa                            <  2:2641557/148‑1 (MQ=255)
                                                                                                                   cAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGa                            <  2:1585586/148‑1 (MQ=255)
                                                                                                                   cAGCAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGa                            <  2:1886201/148‑1 (MQ=255)
                                                                                                                      cAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGc                         >  2:2585890/1‑148 (MQ=255)
                                                                                                                      cAACACCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGc                         >  2:2585878/1‑148 (MQ=255)
                                                                                                                         cacCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCAtt                      >  1:630913/1‑148 (MQ=255)
                                                                                                                         cacCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCAtt                      >  1:2390645/1‑148 (MQ=255)
                                                                                                                         cacCATCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCAtt                      >  1:1491597/1‑148 (MQ=255)
                                                                                                                             aTCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCccg                  >  1:2306445/1‑148 (MQ=255)
                                                                                                                              tCACCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCccgc                 >  1:2921914/1‑148 (MQ=255)
                                                                                                                                aCCAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCccgccg               >  2:2981485/1‑148 (MQ=255)
                                                                                                                                 ccAGCCCGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCcgccgc              >  1:1491288/1‑148 (MQ=255)
                                                                                                                                       cGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCgg        <  1:948062/148‑1 (MQ=255)
                                                                                                                                       cGGCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCgg        <  1:948085/148‑1 (MQ=255)
                                                                                                                                         gCCACCAGTCGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAg      <  2:2186974/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
ATAGCGCCCTGCCCCAGCGGCTAAAAAACCCGCCCTGTGCTTCCGGTATGTCAATTTCGACCGGTGCCTGCGTTTGCGGTGTTTCACGCACCCATTTTTGCACCAGCGTCGCAATCAGCAACACCATCACCAGCCCGGCCACCAGACGAATCGCCGCAAAACCCCAGCCGAGGACAAAGCCCATAAACACCAGCGTCGCCGGGTTTAACACCGGATTGCCCATCCAGAATGCCAGCGCACCGCCCATCGACACCTGTTGGCGACGCATTCCCGCCGCGACCGGAGCCGC  >  CP009273/3290825‑3291113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑