Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,302,027 G→T 100% S86* (TCG→TAG)  nlpI ← lipoprotein involved in osmotic sensitivity and filamentation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,302,0270GT100.0% 763.5 / NA 230S86* (TCG→TAG) nlpIlipoprotein involved in osmotic sensitivity and filamentation
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (124/106);  total (124/106)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 230 total aligned reads displayed.

AAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTA  >  CP009273/3301883‑3302171
                                                                                                                                                |                                                                                                                                                
aaGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaa                                                                                                                                               <  1:716713/148‑1 (MQ=255)
 aGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaaa                                                                                                                                              >  2:798665/1‑148 (MQ=255)
 aGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTaaaa                                                                                                                                              >  1:888179/1‑148 (MQ=255)
   tGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAAt                                                                                                                                            >  2:1038313/1‑148 (MQ=255)
   tGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAAt                                                                                                                                            >  1:3277918/1‑148 (MQ=255)
     cgcgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCg                                                                                                                                          >  2:1779681/1‑148 (MQ=255)
       cgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGtt                                                                                                                                        >  2:2594718/1‑148 (MQ=255)
       cgTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGtt                                                                                                                                        <  2:2882502/148‑1 (MQ=255)
        gTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTa                                                                                                                                       <  2:1731866/148‑1 (MQ=255)
         tAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAc                                                                                                                                      >  1:3309917/1‑148 (MQ=255)
          aGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTAcg                                                                                                                                     <  1:1732792/148‑1 (MQ=255)
                  gTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATg                                                                                                                             >  1:153670/1‑148 (MQ=255)
                   ttGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGc                                                                                                                            <  2:3177798/148‑1 (MQ=255)
                     ggATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATAATTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGccc                                                                                                                          <  1:1021449/148‑1 (MQ=255)
                      gATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCt                                                                                                                         <  1:1286000/148‑1 (MQ=255)
                       aTCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTc                                                                                                                        <  2:1163752/148‑1 (MQ=255)
                        tCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCa                                                                                                                       >  1:1676499/1‑148 (MQ=255)
                         cAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCGTGCTAAAAATCGTTACGCGCTCATGCCCTCAg                                                                                                                      >  1:2111992/1‑148 (MQ=255)
                          aaGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGa                                                                                                                     <  1:57262/148‑1 (MQ=255)
                             cTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCg                                                                                                                  <  1:543227/148‑1 (MQ=255)
                             cTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCg                                                                                                                  >  1:1438352/1‑148 (MQ=255)
                                  gTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACt                                                                                                             >  1:2319765/1‑148 (MQ=255)
                                  gTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACt                                                                                                             <  2:1643168/148‑1 (MQ=255)
                                    aCAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTAt                                                                                                           <  2:1293544/148‑1 (MQ=255)
                                      aGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCa                                                                                                         >  2:3083197/1‑148 (MQ=255)
                                       gAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCAt                                                                                                        <  1:2924367/148‑1 (MQ=255)
                                        aaTCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATa                                                                                                       <  2:1092455/148‑1 (MQ=255)
                                         aTCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATac                                                                                                      >  2:2106543/1‑148 (MQ=255)
                                            aaaCGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATacaac                                                                                                   <  1:1863333/148‑1 (MQ=255)
                                               cGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACt                                                                                                >  2:1975202/1‑148 (MQ=255)
                                               cGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACt                                                                                                >  2:2831021/1‑148 (MQ=255)
                                                gCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTc                                                                                               <  2:153670/148‑1 (MQ=255)
                                                  ttCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCcg                                                                                             >  2:2834766/1‑148 (MQ=255)
                                                  ttCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCcg                                                                                             >  2:490135/1‑148 (MQ=255)
                                                   tCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCcgc                                                                                            <  2:1969143/148‑1 (MQ=255)
                                                   tCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCcgc                                                                                            >  1:1480328/1‑148 (MQ=255)
                                                      tAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCt                                                                                         <  1:2517599/148‑1 (MQ=255)
                                                      tAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCt                                                                                         <  1:3214828/148‑1 (MQ=255)
                                                       aGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTc                                                                                        <  1:2448620/148‑1 (MQ=255)
                                                        ggCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCa                                                                                       <  1:2944046/148‑1 (MQ=255)
                                                        ggCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCa                                                                                       >  1:1510810/1‑148 (MQ=255)
                                                         gcagcaTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCat                                                                                      >  1:3420788/1‑148 (MQ=255)
                                                           agcaTCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCatat                                                                                    <  1:1043279/148‑1 (MQ=255)
                                                               tCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATaaaa                                                                                >  1:142159/1‑148 (MQ=255)
                                                               tCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATaaaa                                                                                >  2:3323464/1‑148 (MQ=255)
                                                                cAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAg                                                                               >  1:70197/1‑148 (MQ=255)
                                                                cAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAg                                                                               >  1:2693235/1‑148 (MQ=255)
                                                                  aaaTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCt                                                                             <  1:3140458/148‑1 (MQ=255)
                                                                  aaaTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCt                                                                             >  2:2660497/1‑148 (MQ=255)
                                                                   aaTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCtg                                                                            <  2:450362/148‑1 (MQ=255)
                                                                   aaTTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCtg                                                                            >  1:170305/1‑148 (MQ=255)
                                                                          tgcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGt                                                                     <  1:1273317/148‑1 (MQ=255)
                                                                          tgcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGt                                                                     <  1:3021347/148‑1 (MQ=255)
                                                                          tgcctgcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGt                                                                     <  1:1914897/148‑1 (MQ=255)
                                                                               gcGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTc                                                                >  2:3297946/1‑148 (MQ=255)
                                                                                  ttAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATc                                                             >  1:1524952/1‑148 (MQ=255)
                                                                                   tAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCg                                                            >  1:3474109/1‑148 (MQ=255)
                                                                                     aaTATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGt                                                          >  1:2038516/1‑148 (MQ=255)
                                                                                      atatatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGtt                                                         >  1:1370/1‑148 (MQ=255)
                                                                                      atatatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGtt                                                         >  1:2145392/1‑148 (MQ=255)
                                                                                        atatGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTaa                                                       >  2:957600/1‑148 (MQ=255)
                                                                                          atGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAg                                                     <  2:2191012/148‑1 (MQ=255)
                                                                                          atGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAg                                                     <  2:2547870/148‑1 (MQ=255)
                                                                                             ccTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGccc                                                  >  1:1794582/1‑148 (MQ=255)
                                                                                               tAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGa                                                >  2:2648724/1‑148 (MQ=255)
                                                                                               tAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGa                                                >  2:2424350/1‑148 (MQ=255)
                                                                                                aaGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATAAAACACTCCGCGCTCATATAATAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGAc                                               >  2:17582/1‑148 (MQ=255)
                                                                                                     aTTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCa                                          <  2:2253343/148‑1 (MQ=255)
                                                                                                      ttGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCaa                                         >  2:1933462/1‑148 (MQ=255)
                                                                                                       tGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAg                                        >  1:2587938/1‑148 (MQ=255)
                                                                                                         aaTACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGa                                      <  1:1240910/148‑1 (MQ=255)
                                                                                                         aaTACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGa                                      >  2:2173028/1‑148 (MQ=255)
                                                                                                          aTACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGAt                                     <  2:2781057/148‑1 (MQ=255)
                                                                                                           tACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATaaaa                                                                                >  2:552475/1‑104 (MQ=255)
                                                                                                           tACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATaaaa                                                                                <  1:838507/104‑1 (MQ=255)
                                                                                                           tACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGAtt                                    <  1:2564735/148‑1 (MQ=255)
                                                                                                            aCTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGAttt                                   >  1:1267414/1‑148 (MQ=255)
                                                                                                             cTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTg                                  >  2:2389349/1‑148 (MQ=255)
                                                                                                              ttCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGt                                 >  2:1207839/1‑148 (MQ=255)
                                                                                                              ttCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGAAAGGATTTGt                                 <  1:669620/148‑1 (MQ=255)
                                                                                                              ttCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCAGACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGt                                 <  2:1880186/148‑1 (MQ=255)
                                                                                                               tCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGtt                                <  1:383408/148‑1 (MQ=255)
                                                                                                               tCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGtt                                <  1:1048762/148‑1 (MQ=255)
                                                                                                               tCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGtt                                >  1:276548/1‑148 (MQ=255)
                                                                                                               tCAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGtt                                <  1:2650755/148‑1 (MQ=255)
                                                                                                                cAGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTc                               <  1:2547549/148‑1 (MQ=255)
                                                                                                                 aGGCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTcc                              <  2:227917/148‑1 (MQ=255)
                                                                                                                  ggCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCa                             <  2:893326/148‑1 (MQ=255)
                                                                                                                  ggCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCa                             >  2:2250014/1‑148 (MQ=255)
                                                                                                                  ggCATATCCGGTCGGAGTGCCAGCGCTTGCTAAAAATCGGTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCa                             <  2:1038779/148‑1 (MQ=255)
                                                                                                                   gCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCAt                            >  1:610229/1‑148 (MQ=255)
                                                                                                                   gCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCAt                            <  1:1978602/148‑1 (MQ=255)
                                                                                                                   gCATATCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTGCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCAt                            >  2:2932268/1‑148 (MQ=255)
                                                                                                                     atatCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATAc                          <  2:327150/148‑1 (MQ=255)
                                                                                                                      tatCCGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACg                         >  1:2828020/1‑148 (MQ=255)
                                                                                                                         ccGGTCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGc                      >  1:3382006/1‑148 (MQ=255)
                                                                                                                             tCGGATTGCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGa                  >  2:3069301/1‑148 (MQ=255)
                                                                                                                                    gCCAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACtt           >  2:271988/1‑148 (MQ=255)
                                                                                                                                     ccAGCGCTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTc          <  2:1511261/148‑1 (MQ=255)
                                                                                                                                        gcgcTTGCTAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCctg       >  1:2387749/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AAGTGCGCGTAGTTGTAAGTTGGATCAAGCTCAAGTACAGAATCAAACGCTTCATAGGCAGCATCAAAATTGCCTGCCTGCGTTAAATATATGCCTAAGTAATTGAATACTTCAGGCATATCCGGTCGGATTGCCAGCGCTTGCGAAAAATCGTTACGCGCTAATGCCCTCAGACCGAGACTATCATACAACACTCCGCGCTCATATAAAAGCTGTGCGCGTTCGTCATCGGTTAAAGCCCGACTGGCAAGGATTTGTTCCATACGTGCCAGAATCACTTCCTGCTGTA  >  CP009273/3301883‑3302171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑