Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,306,165 C→A 100% V125F (GTT→TTT)  rbfA ← 30s ribosome binding factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,306,1650CA99.5% 734.0 / ‑4.3 228V125F (GTT→TTT) rbfA30s ribosome binding factor
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (132/94);  total (133/95)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 232 total aligned reads displayed.

GTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAGA  >  CP009273/3306020‑3306312
                                                                                                                                                 |                                                                                                                                                   
gTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAaaac                                                                                                                                                   <  2:3099128/148‑1 (MQ=255)
  tGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacga                                                                                                                                                 <  2:3489140/148‑1 (MQ=255)
  tGGCGGTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAacga                                                                                                                                                 >  1:3095083/1‑148 (MQ=255)
       ttATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGttc                                                                                                                                            >  1:1594138/1‑148 (MQ=255)
       ttATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGttc                                                                                                                                            >  1:1595375/1‑148 (MQ=255)
          tatataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGttcttc                                                                                                                                         >  1:2744931/1‑148 (MQ=255)
          tatataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGttcttc                                                                                                                                         >  1:136372/1‑148 (MQ=255)
           atataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCg                                                                                                                                        >  2:2573590/1‑148 (MQ=255)
             ataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGTTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTc                                                                                                                                      <  1:173818/148‑1 (MQ=255)
             ataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTc                                                                                                                                      <  2:1410134/148‑1 (MQ=255)
             ataCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTc                                                                                                                                      >  1:1828468/1‑148 (MQ=255)
               aCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCAt                                                                                                                                    >  2:1058417/1‑148 (MQ=255)
               aCGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCAt                                                                                                                                    >  1:887403/1‑148 (MQ=255)
                  tttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGtt                                                                                                                                 >  1:976621/1‑148 (MQ=255)
                  tttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGtt                                                                                                                                 >  2:1476070/1‑148 (MQ=255)
                   ttCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGttt                                                                                                                                >  1:3505629/1‑148 (MQ=255)
                     cACTTTTTGCAGCGCATGGTTGCTGGACATACCGTGAGGTTTATCCAGCAAAAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGa                                                                                                                              <  1:3321734/148‑1 (MQ=255)
                        tttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCa                                                                                                                           >  2:728924/1‑148 (MQ=255)
                         ttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCAc                                                                                                                          >  1:918368/1‑148 (MQ=255)
                         ttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCAc                                                                                                                          >  1:2673392/1‑148 (MQ=255)
                         ttttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCAc                                                                                                                          >  1:329848/1‑148 (MQ=255)
                          tttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACg                                                                                                                         <  2:2297745/148‑1 (MQ=255)
                          tttGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACg                                                                                                                         <  2:3207962/148‑1 (MQ=255)
                               aGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGt                                                                                                                    <  2:2891080/148‑1 (MQ=255)
                                   cATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAcc                                                                                                                >  2:1689080/1‑148 (MQ=255)
                                   cATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAcc                                                                                                                >  1:2482997/1‑148 (MQ=255)
                                   cATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAcc                                                                                                                <  2:609085/148‑1 (MQ=255)
                                   cATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCAcc                                                                                                                >  1:1150600/1‑148 (MQ=255)
                                     tCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAg                                                                                                              >  1:3101838/1‑148 (MQ=255)
                                         tgctgcACATACCCTGAGTTTAATCCACAAAAAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGttt                                                                                                          <  1:2940396/148‑1 (MQ=255)
                                           cTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGa                                                                                                        >  2:1765710/1‑148 (MQ=255)
                                            tGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGAc                                                                                                       >  2:1050082/1‑148 (MQ=255)
                                             ggACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACa                                                                                                      >  2:3316540/1‑148 (MQ=255)
                                              gACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACAt                                                                                                     >  2:786134/1‑148 (MQ=255)
                                               aCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATg                                                                                                    >  1:2373242/1‑148 (MQ=255)
                                               aCATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATg                                                                                                    >  2:1638489/1‑148 (MQ=255)
                                                       tGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATccc                                                                                            >  1:1527991/1‑148 (MQ=255)
                                                        gAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCt                                                                                           >  2:2669925/1‑148 (MQ=255)
                                                         aGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCtt                                                                                          >  1:3243080/1‑148 (MQ=255)
                                                          ggTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTc                                                                                         >  1:1896869/1‑148 (MQ=255)
                                                          ggTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTGGACATGCGCATCGCTTc                                                                                         >  2:1865594/1‑148 (MQ=255)
                                                            tttATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCaa                                                                                       >  1:2960871/1‑148 (MQ=255)
                                                                tCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCag                                                                                   >  1:372476/1‑148 (MQ=255)
                                                                tCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCag                                                                                   <  1:210537/148‑1 (MQ=255)
                                                                 ccAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCaga                                                                                  >  1:2887628/1‑148 (MQ=255)
                                                                  catcaacaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagag                                                                                 <  2:2945958/145‑1 (MQ=255)
                                                                   aGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagaga                                                                                <  2:3065007/148‑1 (MQ=255)
                                                                   aGCAAAAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagaga                                                                                <  1:795586/148‑1 (MQ=255)
                                                                    gCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCagagag                                                                               <  1:2626083/148‑1 (MQ=255)
                                                                     caacaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGt                                                                              <  1:546964/148‑1 (MQ=255)
                                                                       acaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTg                                                                            <  2:2804103/148‑1 (MQ=255)
                                                                       acaaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTg                                                                            >  1:1123022/1‑148 (MQ=255)
                                                                        caaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGt                                                                           >  2:481252/1‑148 (MQ=255)
                                                                        caaAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGt                                                                           <  1:945913/148‑1 (MQ=255)
                                                                         aaaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTc                                                                          <  1:1484751/148‑1 (MQ=255)
                                                                         aaaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTc                                                                          >  1:1024886/1‑148 (MQ=255)
                                                                          aaaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCg                                                                         >  1:1671550/1‑148 (MQ=255)
                                                                           aaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGt                                                                        >  1:320546/1‑148 (MQ=255)
                                                                           aaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGt                                                                        >  2:474952/1‑148 (MQ=255)
                                                                           aaCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGt                                                                        >  2:821442/1‑148 (MQ=255)
                                                                            aCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTa                                                                       >  2:2682040/1‑148 (MQ=255)
                                                                            aCGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTa                                                                       >  2:846018/1‑148 (MQ=255)
                                                                              gCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTaga                                                                     <  1:1539911/148‑1 (MQ=255)
                                                                               ccGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTagaa                                                                    >  1:3185042/1‑148 (MQ=255)
                                                                                cGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTagaag                                                                   >  1:1345981/1‑148 (MQ=255)
                                                                                cGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTagaag                                                                   <  2:2254635/148‑1 (MQ=255)
                                                                                 gTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTagaaga                                                                  <  1:1301995/148‑1 (MQ=255)
                                                                                     aTGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGt                                                              <  1:608709/148‑1 (MQ=255)
                                                                                        tCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAg                                                           >  1:1982651/1‑148 (MQ=255)
                                                                                          gcgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGtt                                                         <  1:2272694/148‑1 (MQ=255)
                                                                                          gcgaccgcgacGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGtt                                                         <  1:1638489/148‑1 (MQ=255)
                                                                                                   acgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCATGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGa                                                <  2:1758029/148‑1 (MQ=255)
                                                                                                   acgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGa                                                <  2:1911948/148‑1 (MQ=255)
                                                                                                    cgacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGAt                                               <  2:1166234/148‑1 (MQ=255)
                                                                                                     gacgaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTGACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATa                                              <  2:2956802/148‑1 (MQ=255)
                                                                                                        gaGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGc                                           <  2:705362/148‑1 (MQ=255)
                                                                                                         aGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCa                                          <  1:1765710/148‑1 (MQ=255)
                                                                                                           gacgacTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAgg                                        <  2:2588662/148‑1 (MQ=255)
                                                                                                            acgacTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGgc                                       >  1:3463039/1‑148 (MQ=255)
                                                                                                                    ttAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCt                               >  2:2534343/1‑148 (MQ=255)
                                                                                                                    ttAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCt                               >  2:3048124/1‑148 (MQ=255)
                                                                                                                    ttAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCt                               <  1:3059335/148‑1 (MQ=255)
                                                                                                                    ttAGTCCTCCTTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCACTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTACGGCACGATACGCCGGCGCATCGCt                               >  2:3148583/1‑148 (MQ=255)
                                                                                                                        tcctccTTGCTGTCGTCCGGGTTAAAACGTAGTTCTTGGTCATGTTTGACCACGGTTGTCACCAGGTTTGAAATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTcc                           <  2:2175421/148‑1 (MQ=255)
                                                                                                                         cctcctTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTccc                          >  1:1236137/1‑148 (MQ=255)
                                                                                                                          ctcctTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTcccc                         <  2:1129561/148‑1 (MQ=255)
                                                                                                                           tcctTGCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCca                        >  2:2750447/1‑148 (MQ=255)
                                                                                                                                gCTGTCGTCCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAgg                   <  1:2424644/148‑1 (MQ=255)
                                                                                                                                    tcgtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGc               >  2:857062/1‑148 (MQ=255)
                                                                                                                                      gtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCgg             <  1:970832/148‑1 (MQ=255)
                                                                                                                                      gtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCgg             <  2:509130/148‑1 (MQ=255)
                                                                                                                                      gtcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCgg             <  2:1595124/148‑1 (MQ=255)
                                                                                                                                       tcCGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGa            >  2:269508/1‑148 (MQ=255)
                                                                                                                                        ccGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGAt           >  2:633918/1‑148 (MQ=255)
                                                                                                                                         cGGGTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATg          <  1:728924/148‑1 (MQ=255)
                                                                                                                                           ggTTAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGaa        <  2:2031413/148‑1 (MQ=255)
                                                                                                                                             ttAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAAc      >  2:2615355/1‑148 (MQ=255)
                                                                                                                                              tAAAACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAAcc     <  1:2290880/148‑1 (MQ=255)
                                                                                                                                                 cacGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAGa  >  2:3344426/1‑148 (MQ=255)
                                                                                                                                                 cacGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAGa  >  2:176963/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
GTTGGCGTTATATATACGTTTCACTTTTTGCAGCGCATCGTTGCTGGACATACCCTGAGGTTTATCCAGCAACAAAACGCCGTTAATGTCGCGACCGCGACGACGAGGACGACTCATTAGTCCTCCTTGCTGTCGTCCGGGTTAACACGACGTTCTTCGTCATGTTTGACCACGCTGGTCACCAGGTTTGACATGCGCATCCCTTCAACCAGAGAGTTGTCGTAGAAGAAGGTCAGTTCCGGCACGATACGCAGGCGCATCGCTTTCCCCAGCAGGCTGCGGATGAAACCAGA  >  CP009273/3306020‑3306312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑