Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,334,687 G→T 100% A21A (GCG→GCT)  kdsD → D‑arabinose 5‑phosphate isomerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,334,6870GT99.0% 674.7 / ‑1.8 205A21A (GCG→GCT) kdsDD‑arabinose 5‑phosphate isomerase
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (116/87);  total (117/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

ATTATTAACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCA  >  CP009273/3334542‑3334831
                                                                                                                                                 |                                                                                                                                                
    ttaACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGaa                                                                                                                                            >  1:562485/1‑148 (MQ=255)
    ttaACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGaa                                                                                                                                            >  1:561922/1‑148 (MQ=255)
       aCTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAGTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGt                                                                                                                                         <  1:2933117/148‑1 (MQ=255)
       aCTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGt                                                                                                                                         <  1:1655557/148‑1 (MQ=255)
         tGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGa                                                                                                                                       >  1:940741/1‑148 (MQ=255)
          ggCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGaa                                                                                                                                      <  1:2704296/148‑1 (MQ=255)
          ggCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGaa                                                                                                                                      >  1:3415682/1‑148 (MQ=255)
          ggCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGaa                                                                                                                                      <  1:155488/148‑1 (MQ=255)
            cGGATTTATCGTATGGCTGGCGATGTTGTACTGGTGATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATg                                                                                                                                    <  2:2146601/148‑1 (MQ=255)
                 ttATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTgg                                                                                                                               <  2:1587884/148‑1 (MQ=255)
                 ttATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTgg                                                                                                                               <  1:1707650/148‑1 (MQ=255)
                     cGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGa                                                                                                                           >  2:1325815/1‑148 (MQ=255)
                     cGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGa                                                                                                                           >  2:480049/1‑148 (MQ=255)
                       tATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTCGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGc                                                                                                                         >  1:2033722/1‑148 (MQ=255)
                         tggctggcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCtt                                                                                                                       >  1:2860260/1‑148 (MQ=255)
                           gctggcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGa                                                                                                                     <  2:319527/148‑1 (MQ=255)
                             tggcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATc                                                                                                                   >  2:1735425/1‑148 (MQ=255)
                               gcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCaa                                                                                                                 >  1:1905919/1‑148 (MQ=255)
                               gcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCaa                                                                                                                 >  1:129935/1‑148 (MQ=255)
                               gcGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCaa                                                                                                                 >  1:129850/1‑148 (MQ=255)
                                cGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAAt                                                                                                                <  2:774599/148‑1 (MQ=255)
                                cGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAAt                                                                                                                <  1:3076929/148‑1 (MQ=255)
                                   tgttgtACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACa                                                                                                             >  2:2638969/1‑148 (MQ=255)
                                    gttgtACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACat                                                                                                            <  1:1724977/148‑1 (MQ=255)
                                    gttgtACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACat                                                                                                            >  2:3316082/1‑148 (MQ=255)
                                        tACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACatcaat                                                                                                        <  2:3237469/148‑1 (MQ=255)
                                         aCTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACatcaatc                                                                                                       <  2:2351517/148‑1 (MQ=255)
                                           tGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAg                                                                                                     >  1:1099334/1‑148 (MQ=255)
                                            ggTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGATTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGa                                                                                                    <  2:1189907/148‑1 (MQ=255)
                                            ggTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGa                                                                                                    >  1:160859/1‑148 (MQ=255)
                                              ttATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAAt                                                                                                  <  1:2907659/148‑1 (MQ=255)
                                                     cAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGc                                                                                           >  1:853366/1‑148 (MQ=255)
                                                      aaTACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCt                                                                                          <  1:235461/148‑1 (MQ=255)
                                                        tACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTg                                                                                        >  2:262053/1‑148 (MQ=255)
                                                         aCTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGc                                                                                       >  2:1048890/1‑148 (MQ=255)
                                                         aCTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGc                                                                                       >  2:2485563/1‑148 (MQ=255)
                                                         aCTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGc                                                                                       >  1:2583775/1‑148 (MQ=255)
                                                           tCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCt                                                                                     >  1:1364260/1‑148 (MQ=255)
                                                            cGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCtg                                                                                    >  1:670227/1‑148 (MQ=255)
                                                               tGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGa                                                                                 <  2:2886181/148‑1 (MQ=255)
                                                                gAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaa                                                                                >  1:839213/1‑148 (MQ=255)
                                                                 aaTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaaa                                                                               >  2:1028406/1‑148 (MQ=255)
                                                                 aaTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaaa                                                                               >  2:366251/1‑148 (MQ=255)
                                                                  aTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaaaa                                                                              >  1:1534414/1‑148 (MQ=255)
                                                                  aTAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGaaaa                                                                              >  2:1683322/1‑148 (MQ=255)
                                                                      cTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATg                                                                          <  1:2638969/148‑1 (MQ=255)
                                                                      cTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATg                                                                          >  1:1748787/1‑148 (MQ=255)
                                                                      cTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATg                                                                          >  2:1405415/1‑148 (MQ=255)
                                                                      cTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATg                                                                          >  1:3307740/1‑148 (MQ=255)
                                                                      cTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATg                                                                          <  1:2968257/148‑1 (MQ=255)
                                                                         gAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTc                                                                       >  2:458677/1‑148 (MQ=255)
                                                                         gAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTc                                                                       >  2:2617941/1‑148 (MQ=255)
                                                                          aaaCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCt                                                                      >  1:304451/1‑148 (MQ=255)
                                                                            aCGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTgg                                                                    >  2:2067492/1‑148 (MQ=255)
                                                                              gCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGgtg                                                                  <  1:476235/148‑1 (MQ=255)
                                                                              gCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGgtg                                                                  >  2:2619646/1‑148 (MQ=255)
                                                                                aTTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTTAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTa                                                                >  1:871331/1‑148 (MQ=255)
                                                                                aTTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTa                                                                >  1:1513986/1‑148 (MQ=255)
                                                                                  tATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTaaa                                                              <  2:980242/148‑1 (MQ=255)
                                                                                  tATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTaaa                                                              <  1:915816/148‑1 (MQ=255)
                                                                                  tATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTaaa                                                              <  2:3297448/148‑1 (MQ=255)
                                                                                  tATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTaaa                                                              <  2:1230746/148‑1 (MQ=255)
                                                                                     gTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAggg                                                           >  1:278320/1‑148 (MQ=255)
                                                                                      tCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGa                                                          >  1:1356117/1‑148 (MQ=255)
                                                                                       cGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGaa                                                         <  1:1044973/148‑1 (MQ=255)
                                                                                         cACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGACAAGAGGTTCTGGTGTAAAGGGACAg                                                       >  2:3084569/1‑148 (MQ=255)
                                                                                          aCGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGt                                                      <  1:260397/148‑1 (MQ=255)
                                                                                           cGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGtt                                                     >  1:700782/1‑148 (MQ=255)
                                                                                            gTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTg                                                    <  1:486266/148‑1 (MQ=255)
                                                                                            gTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTg                                                    <  1:486196/148‑1 (MQ=255)
                                                                                             tAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTgt                                                   >  1:132489/1‑148 (MQ=255)
                                                                                                      aaCCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATggg                                          >  2:1721338/1‑148 (MQ=255)
                                                                                                          gggTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATg                                      >  1:3152437/1‑148 (MQ=255)
                                                                                                          gggTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATg                                      >  1:3313412/1‑148 (MQ=255)
                                                                                                           ggTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATgg                                     <  2:1675308/148‑1 (MQ=255)
                                                                                                           ggTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATgg                                     <  2:33965/148‑1 (MQ=255)
                                                                                                           ggTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATgg                                     >  2:2181446/1‑148 (MQ=255)
                                                                                                            gTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATggg                                    >  1:3456107/1‑148 (MQ=255)
                                                                                                              tttGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGaa                                  >  2:1619925/1‑148 (MQ=255)
                                                                                                              tttGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGaa                                  >  2:1619745/1‑148 (MQ=255)
                                                                                                                 gACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAAt                               <  2:260501/148‑1 (MQ=255)
                                                                                                                     ttCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCggg                           <  2:561922/148‑1 (MQ=255)
                                                                                                                     ttCAGCAAGCAGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCggg                           <  2:562485/148‑1 (MQ=255)
                                                                                                                        agcaagcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCa                        <  1:1412598/148‑1 (MQ=255)
                                                                                                                         gcaagcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCat                       <  2:853366/148‑1 (MQ=255)
                                                                                                                         gcaagcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCat                       >  1:21104/1‑148 (MQ=255)
                                                                                                                          caagcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCata                      <  2:682538/148‑1 (MQ=255)
                                                                                                                          caagcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCGTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGGTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCata                      <  2:10766/148‑1 (MQ=255)
                                                                                                                            agcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATAtt                    >  2:2942659/1‑148 (MQ=255)
                                                                                                                            agcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATAtt                    >  1:3156382/1‑148 (MQ=255)
                                                                                                                             gcaTGTAAAGAAGTACTGGCTAGTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACTCTTGCCTGAGACAAGATGTTCTTGTGTAAAGGGAAATTTGTCGTCATGGTGATGGGAAAATCGGTGCATATTg                   >  2:2311924/1‑148 (MQ=255)
                                                                                                                             gcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTg                   <  2:634115/148‑1 (MQ=255)
                                                                                                                             gcaGGTAAAGAAGTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTg                   <  2:1656616/148‑1 (MQ=255)
                                                                                                                                   aaaGAAGTCCTGGCTCTGGAACGTGAATGCCTGGCGGAGCTTGATCCATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGTTGGTCGGGTGGCACGGGAAGGTTGTCGTCAGGGGGAGGGGCATTGCGGGGCATCGTGGGCGaa             >  2:608704/1‑148 (MQ=255)
                                                                                                                                     agaagTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGaaaa           >  1:2637828/1‑148 (MQ=255)
                                                                                                                                        agTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATg        <  2:2561509/148‑1 (MQ=255)
                                                                                                                                        agTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATg        >  1:735869/1‑148 (MQ=255)
                                                                                                                                         gTCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATgg       >  1:2292264/1‑148 (MQ=255)
                                                                                                                                          tCCTGGCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATggc      >  2:3225182/1‑148 (MQ=255)
                                                                                                                                              ggCTATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCa  <  1:2493462/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
ATTATTAACTGGCGGATTTATCGTATGGCTGGCGATGTTGTACTGGTTATCGCCAATACTCGTTGAATAACTGGAAACGCATTATGTCGCACGTAGAGTTACAACCGGGTTTTGACTTTCAGCAAGCAGGTAAAGAAGTCCTGGCGATTGAACGTGAATGCCTGGCGGAGCTTGATCAATACATCAATCAGAATTTCACGCTTGCCTGTGAAAAGATGTTCTGGTGTAAAGGGAAAGTTGTCGTCATGGGGATGGGAAAATCGGGGCATATTGGGCGAAAAATGGCGGCA  >  CP009273/3334542‑3334831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑