Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,351,650 G→T 100% E1189D (GAG→GAT)  gltB → glutamate synthase, large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,351,6500GT99.4% 567.4 / ‑4.2 176E1189D (GAG→GAT) gltBglutamate synthase, large subunit
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (92/82);  total (94/82)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 176 total aligned reads displayed.

TTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATAT  >  CP009273/3351507‑3351793
                                                                                                                                               |                                                                                                                                               
     cGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGaa                                                                                                                                        >  1:1348839/1‑148 (MQ=255)
     cGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGaa                                                                                                                                        <  2:2493283/148‑1 (MQ=255)
     cGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGaa                                                                                                                                        >  1:3184714/1‑148 (MQ=255)
        ccGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCg                                                                                                                                     <  1:2277684/148‑1 (MQ=255)
         cGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGc                                                                                                                                    >  2:2163804/1‑148 (MQ=255)
          gTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCa                                                                                                                                   >  1:2162039/1‑148 (MQ=255)
            gAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATc                                                                                                                                 <  2:1652972/148‑1 (MQ=255)
            gAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATc                                                                                                                                 >  1:992789/1‑148 (MQ=255)
            gAAACCCGCGAGCGGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTTGCGCTGTCGAAGCTGCTGGATACTGCAGAACCGCATc                                                                                                                                 >  1:3101609/1‑148 (MQ=255)
             aaaCCCGCGAGCGGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATcc                                                                                                                                <  2:3479419/148‑1 (MQ=255)
              aaCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCa                                                                                                                               <  2:889380/148‑1 (MQ=255)
              aaCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCa                                                                                                                               <  2:1015197/148‑1 (MQ=255)
               aCCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGTTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAg                                                                                                                              <  2:1660948/148‑1 (MQ=255)
                cccGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAgg                                                                                                                             <  1:3101258/148‑1 (MQ=255)
                cccGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAgg                                                                                                                             >  2:2023923/1‑148 (MQ=255)
                cccGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAgg                                                                                                                             >  2:2928262/1‑148 (MQ=255)
                  cgcgAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTa                                                                                                                           <  2:519639/148‑1 (MQ=255)
                      aGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGc                                                                                                                       >  1:706048/1‑148 (MQ=255)
                       gCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCa                                                                                                                      <  2:837001/148‑1 (MQ=255)
                         tGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCAct                                                                                                                    <  1:3087112/148‑1 (MQ=255)
                         tGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCAct                                                                                                                    >  2:2695447/1‑148 (MQ=255)
                           aTGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCActct                                                                                                                  >  1:1332353/1‑148 (MQ=255)
                           aTGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCActct                                                                                                                  >  2:439550/1‑148 (MQ=255)
                                acaGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGc                                                                                                             <  1:2006365/148‑1 (MQ=255)
                                   gCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCAcc                                                                                                          >  2:1218708/1‑148 (MQ=255)
                                   gCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCAcc                                                                                                          >  2:1068149/1‑148 (MQ=255)
                                    cTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCg                                                                                                         >  1:761842/1‑148 (MQ=255)
                                       ggCGTAACACGTCTGGTGGATCTGATTGGTCGCACCTACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaa                                                                                                      <  2:3113238/148‑1 (MQ=255)
                                        gCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCATCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGaaaa                                                                                                     <  1:2321569/148‑1 (MQ=255)
                                          gTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAaaca                                                                                                   <  2:1152448/148‑1 (MQ=255)
                                              cacGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAAccc                                                                                               >  2:3411333/1‑148 (MQ=255)
                                                  tCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCCACTGCACCGAAAACAACccgccg                                                                                           >  2:761747/1‑148 (MQ=255)
                                                     ggtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGttt                                                                                        <  2:454562/148‑1 (MQ=255)
                                                      gtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTg                                                                                       >  2:3012194/1‑148 (MQ=255)
                                                      gtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTg                                                                                       >  2:1799931/1‑148 (MQ=255)
                                                      gtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTg                                                                                       >  2:3211792/1‑148 (MQ=255)
                                                      gtggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTg                                                                                       >  2:1599412/1‑148 (MQ=255)
                                                       tggATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGCAAACAACCCGCCGTTTGa                                                                                      >  2:3046369/1‑148 (MQ=255)
                                                        ggATCTGATTGGTCGCACCGACCTGATGAAAGAGCTGGACGGTATCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGTTACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGACAACAACCCGCCGGTTGAt                                                                                     >  1:1547050/1‑148 (MQ=255)
                                                         gATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATa                                                                                    <  1:791114/148‑1 (MQ=255)
                                                           tCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAAc                                                                                  <  2:585527/148‑1 (MQ=255)
                                                           tCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAAc                                                                                  >  1:2634385/1‑148 (MQ=255)
                                                           tCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAAc                                                                                  >  1:2634369/1‑148 (MQ=255)
                                                           tCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAAc                                                                                  <  2:1104590/148‑1 (MQ=255)
                                                           tCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAAc                                                                                  >  2:3260770/1‑148 (MQ=255)
                                                              gATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGc                                                                               <  2:2585491/148‑1 (MQ=255)
                                                               aTTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                              >  1:1061287/1‑148 (MQ=255)
                                                               aTTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                              <  2:866986/148‑1 (MQ=255)
                                                               aTTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                              <  2:1017888/148‑1 (MQ=255)
                                                               aTTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGcc                                                                              >  1:1061146/1‑148 (MQ=255)
                                                                ttGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCct                                                                             >  1:3387979/1‑148 (MQ=255)
                                                                 tGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCctg                                                                            <  2:2285580/148‑1 (MQ=255)
                                                                        aCCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcg                                                                     >  1:2550462/1‑148 (MQ=255)
                                                                          cGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgcg                                                                   >  1:828853/1‑148 (MQ=255)
                                                                          cGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAAcgcg                                                                   >  1:828852/1‑148 (MQ=255)
                                                                            aCCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCa                                                                 >  2:2824841/1‑148 (MQ=255)
                                                                               tgctgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGtt                                                              >  1:1957421/1‑148 (MQ=255)
                                                                                  tgAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgct                                                           <  2:1992157/148‑1 (MQ=255)
                                                                                   gAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgctg                                                          >  2:1921961/1‑148 (MQ=255)
                                                                                   gAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTtgctg                                                          >  2:1921992/1‑148 (MQ=255)
                                                                                     aaGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCa                                                        >  1:251041/1‑148 (MQ=255)
                                                                                      agagCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCaa                                                       >  2:2196834/1‑148 (MQ=255)
                                                                                        agCTGGACGGTTTCACCGCCACACAGCAGAAGCTGGCGCTGTCGAATCTGCTGGTTACTGACGAACCGCAGCGAGGTAAGGCACTCTTATGCACCGATAACAACACGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACa                                                     <  2:2584758/148‑1 (MQ=255)
                                                                                         gCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAg                                                    >  2:2421684/1‑148 (MQ=255)
                                                                                         gCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAg                                                    >  2:2421732/1‑148 (MQ=255)
                                                                                          cTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAgg                                                   <  2:1118672/148‑1 (MQ=255)
                                                                                             gACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGa                                                >  1:502320/1‑148 (MQ=255)
                                                                                             gACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGa                                                >  2:3352477/1‑148 (MQ=255)
                                                                                             gACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGa                                                >  2:549683/1‑148 (MQ=255)
                                                                                              aCGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGAACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGaa                                               >  2:2696075/1‑148 (MQ=255)
                                                                                                ggTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAAc                                             <  1:2419838/148‑1 (MQ=255)
                                                                                                  tttCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGTATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACATGCGAAACCg                                           <  2:93724/148‑1 (MQ=255)
                                                                                                       ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGt                                      >  1:1656063/1‑148 (MQ=255)
                                                                                                       ccGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGt                                      >  1:1656118/1‑148 (MQ=255)
                                                                                                        cGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTc                                     >  1:516370/1‑148 (MQ=255)
                                                                                                           cAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGAt                                  <  1:3432327/148‑1 (MQ=255)
                                                                                                           cAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGAt                                  <  1:3432411/148‑1 (MQ=255)
                                                                                                            aaaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATg                                 <  2:2145588/148‑1 (MQ=255)
                                                                                                            aaaCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATg                                 <  2:2745082/148‑1 (MQ=255)
                                                                                                              aCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAg                               >  2:370175/1‑148 (MQ=255)
                                                                                                              aCAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAg                               >  1:3025912/1‑148 (MQ=255)
                                                                                                                 gcagAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAgcgc                            <  1:1770300/148‑1 (MQ=255)
                                                                                                                 gcagAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAgcgc                            <  2:778509/148‑1 (MQ=255)
                                                                                                                    gAAGCTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCag                         >  1:2071448/1‑148 (MQ=255)
                                                                                                                        cTGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCa                     <  2:3101582/148‑1 (MQ=255)
                                                                                                                         tGGCGCTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCaa                    <  1:439550/148‑1 (MQ=255)
                                                                                                                            cgcTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAAc                 <  2:2634385/148‑1 (MQ=255)
                                                                                                                             gcTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAAcc                <  1:3278899/148‑1 (MQ=255)
                                                                                                                              cTGTCGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCt               <  2:697676/148‑1 (MQ=255)
                                                                                                                                  cGAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTg           >  2:1092647/1‑148 (MQ=255)
                                                                                                                                   gAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTgg          <  1:1340415/148‑1 (MQ=255)
                                                                                                                                   gAAGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTgg          <  1:1340504/148‑1 (MQ=255)
                                                                                                                                    aaGCTTCTGGATACTGCCGAAGCTGATCCAGGTAATGGACTCTACTGCACCGAAAACATCGCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTTCTGCAACAGTCGTAAGCGTTTGTAGATGAGCGCCATAGCTAAACCTTCTGGt         <  2:3044836/148‑1 (MQ=255)
                                                                                                                                     aGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGtt        >  2:2616735/1‑148 (MQ=255)
                                                                                                                                     aGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGtt        >  2:721138/1‑148 (MQ=255)
                                                                                                                                     aGCTGCTGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGtt        >  2:3117933/1‑148 (MQ=255)
                                                                                                                                      gctgctGGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTc       >  2:1851191/1‑148 (MQ=255)
                                                                                                                                       ctgctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCg      <  1:124886/148‑1 (MQ=255)
                                                                                                                                       ctgctgGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCg      >  1:878838/1‑148 (MQ=255)
                                                                                                                                          ctgGATACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGata   >  2:344037/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                               
TTTATCGCCCGTGAAACCCGCGAGCTGATGGCACAGCTTGGCGTAACACGTCTGGTGGATCTGATTGGTCGCACCGACCTGCTGAAAGAGCTGGACGGTTTCACCGCCAAACAGCAGAAGCTGGCGCTGTCGAAGCTGCTGGAGACTGCCGAACCGCATCCAGGTAAGGCACTCTACTGCACCGAAAACAACCCGCCGTTTGATAACGGCCTGCTGAACGCGCAGTTGCTGCAACAGGCGAAACCGTTTGTCGATGAGCGCCAGAGCAAAACCTTCTGGTTCGATAT  >  CP009273/3351507‑3351793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑