Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,407,969 C→A 100% N249K (AAC→AAA)  acrE → cytoplasmic membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,407,9690CA96.0% 657.0 / 17.8 223N249K (AAC→AAA) acrEcytoplasmic membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (5/4);  new base A (117/96);  total (122/101)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.45e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 225 total aligned reads displayed.

AACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGAT  >  CP009273/3407824‑3408113
                                                                                                                                                 |                                                                                                                                                
aaCTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAgg                                                                                                                                                <  1:841385/148‑1 (MQ=255)
    ggCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCACTCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCaa                                                                                                                                            <  2:1779144/148‑1 (MQ=255)
    ggCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTCACCCAATCCCGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCaa                                                                                                                                            <  1:750697/148‑1 (MQ=255)
     gCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCaaa                                                                                                                                           >  1:3481481/1‑148 (MQ=255)
     gCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCaaa                                                                                                                                           >  2:1761284/1‑148 (MQ=255)
     gCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCaaa                                                                                                                                           <  2:141613/148‑1 (MQ=255)
        aCTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCt                                                                                                                                        <  2:2267254/148‑1 (MQ=255)
         cTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTa                                                                                                                                       >  1:2750810/1‑148 (MQ=255)
           gTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAAGCCGTAGAGCAAGGAAATGTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATc                                                                                                                                     <  2:1873026/148‑1 (MQ=255)
            tCCAGCAGCTCGATCCTAACTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCTTAGAGCAAGGAAATTTGCATAAGCAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATcc                                                                                                                                    <  1:811887/148‑1 (MQ=255)
             ccAGCAGCTCTATCATATCTACGTTTATGTGACCCAAACCAGAAACTATTTTATGAGGCTTAACCAATCCGTAGAGCAAGTAAATTTGCATAAGCAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATccc                                                                                                                                   <  1:2675175/148‑1 (MQ=255)
             ccAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATccc                                                                                                                                   >  2:973878/1‑148 (MQ=255)
              cagcagCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATcccc                                                                                                                                  >  2:930418/1‑148 (MQ=255)
               agcggcTCGATCCTATCTACTTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCACGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCt                                                                                                                                 <  1:60850/148‑1 (MQ=255)
                gcagcTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTg                                                                                                                                >  2:3231710/1‑148 (MQ=255)
                   gcTCCATCCTATCTACTTTTATGTGACCCAATCCAGAAACGTTTTTATGAGGCTGAAGAAATCCTTAGAGCAAGGAAATTTGAATAAGCAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGaaa                                                                                                                             <  1:2668052/148‑1 (MQ=255)
                    cTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAg                                                                                                                            >  2:1474480/1‑148 (MQ=255)
                      cGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGt                                                                                                                          >  2:1093200/1‑148 (MQ=255)
                         tCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACg                                                                                                                       <  1:1077313/148‑1 (MQ=255)
                          ccTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGc                                                                                                                      <  1:179044/148‑1 (MQ=255)
                          ccTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGc                                                                                                                      >  1:577290/1‑148 (MQ=255)
                            tATCTACTTGGATGTCACCCAATCCCCCAACGATTTTATGAGCCGCAACCAATCCCTACAGCAAGGAAATTTGCATAAGGAACACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTg                                                                                                                    <  1:755661/148‑1 (MQ=255)
                            tATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTg                                                                                                                    <  2:1458811/148‑1 (MQ=255)
                            tATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTg                                                                                                                    >  2:3432087/1‑148 (MQ=255)
                             aTCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGc                                                                                                                   >  2:3207541/1‑148 (MQ=255)
                              tCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCa                                                                                                                  >  1:191366/1‑148 (MQ=255)
                              tCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCa                                                                                                                  >  2:3314586/1‑148 (MQ=255)
                               cTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCaa                                                                                                                 >  1:2238421/1‑148 (MQ=255)
                               cTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCaa                                                                                                                 >  2:2845741/1‑148 (MQ=255)
                                   gTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATtct                                                                                                             >  1:3162633/1‑148 (MQ=255)
                                             cccAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGGTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGAc                                                                                                   >  2:1979067/1‑148 (MQ=255)
                                                aaTCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGt                                                                                                >  1:2993483/1‑148 (MQ=255)
                                                aaTCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGt                                                                                                <  2:1758745/148‑1 (MQ=255)
                                                  tCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTATAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTtg                                                                                              <  1:339254/148‑1 (MQ=255)
                                                       cAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGTAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGaa                                                                                         <  1:990343/148‑1 (MQ=255)
                                                       cAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGaa                                                                                         >  1:3334215/1‑148 (MQ=255)
                                                         aCGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAg                                                                                       <  2:1846125/148‑1 (MQ=255)
                                                            aTTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAc                                                                                    >  1:2192037/1‑148 (MQ=255)
                                                             ttttATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAcc                                                                                   >  1:3233088/1‑148 (MQ=255)
                                                             ttttATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAcc                                                                                   <  2:1355848/148‑1 (MQ=255)
                                                             ttttATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAcc                                                                                   <  2:836297/148‑1 (MQ=255)
                                                             ttttATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCAcc                                                                                   >  1:766291/1‑148 (MQ=255)
                                                              tttATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCg                                                                                  >  2:2797022/1‑148 (MQ=255)
                                                                  tGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTc                                                                              >  2:631067/1‑148 (MQ=255)
                                                                      gCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATa                                                                          >  1:2073056/1‑148 (MQ=255)
                                                                          aaGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAAccc                                                                      >  2:3367542/1‑148 (MQ=255)
                                                                          aaGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAAccc                                                                      <  2:3490046/148‑1 (MQ=255)
                                                                          aaGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAAccc                                                                      >  2:536531/1‑148 (MQ=255)
                                                                           aGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCt                                                                     >  1:684310/1‑148 (MQ=255)
                                                                            gCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTa                                                                    >  2:2907188/1‑148 (MQ=255)
                                                                             cAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTAc                                                                   >  1:2183880/1‑148 (MQ=255)
                                                                             cAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTAc                                                                   >  1:319550/1‑148 (MQ=255)
                                                                              aaTCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACg                                                                  <  2:2053508/148‑1 (MQ=255)
                                                                               aTCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGt                                                                 >  1:2032226/1‑148 (MQ=255)
                                                                                   gTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTg                                                             >  2:515883/1‑148 (MQ=255)
                                                                                    tAGAGCAAGTAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGt                                                            <  1:2803129/148‑1 (MQ=255)
                                                                                    tAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGt                                                            <  1:2571334/148‑1 (MQ=255)
                                                                                      gagCACGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCt                                                          >  2:1226526/1‑148 (MQ=255)
                                                                                      gagCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCt                                                          >  1:3335051/1‑148 (MQ=255)
                                                                                       agCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCtt                                                         >  1:2004411/1‑148 (MQ=255)
                                                                                       agCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCtt                                                         <  2:358042/148‑1 (MQ=255)
                                                                                          aaGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTccc                                                      <  2:3392093/148‑1 (MQ=255)
                                                                                           aGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCt                                                     >  1:902172/1‑148 (MQ=255)
                                                                                           aGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCt                                                     >  2:2771501/1‑148 (MQ=255)
                                                                                           aGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCt                                                     >  1:3292433/1‑148 (MQ=255)
                                                                                            ggAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTa                                                    >  1:1859980/1‑148 (MQ=255)
                                                                                             gAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTaa                                                   >  2:2025780/1‑148 (MQ=255)
                                                                                                aTTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAAccc                                                >  2:3090689/1‑148 (MQ=255)
                                                                                                  ttGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGc                                              >  2:314405/1‑148 (MQ=255)
                                                                                                     cATAAGGAAGACGCCAGGAGCATCGTAGAGTTGGTCTTGTAAAATGGAAATTACTATCCCCTGAAAGGTAAGCTGCAATTCTCCGATGTGAGCTTTGTTTAAAGCAACGGCTCCTTATCCCTACGTGCTGTCTTCCCTAACCAGCAAc                                           <  2:2549815/148‑1 (MQ=255)
                                                                                                       tAATGAAAACGCCACCAGCAACGTAGAGTTGGTCATTGAAATAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACAt                                         <  2:487046/148‑1 (MQ=255)
                                                                                                       tAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACAt                                         <  2:2750810/148‑1 (MQ=255)
                                                                                                        aaGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATa                                        <  1:1447942/148‑1 (MQ=255)
                                                                                                           gAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGc                                     >  1:2216674/1‑148 (MQ=255)
                                                                                                           gAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGc                                     <  2:191468/148‑1 (MQ=255)
                                                                                                              aaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCttt                                  <  1:1345257/148‑1 (MQ=255)
                                                                                                              aaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCttt                                  <  2:1258485/148‑1 (MQ=255)
                                                                                                              aaCGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACATATCACCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCttt                                  >  1:3134686/1‑148 (MQ=255)
                                                                                                                cGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTg                                <  1:2542082/148‑1 (MQ=255)
                                                                                                                cGCCACCAGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTg                                >  2:116426/1‑148 (MQ=255)
                                                                                                                    accaGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCgg                            >  1:698315/1‑148 (MQ=255)
                                                                                                                      caGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGt                          >  1:1160848/1‑148 (MQ=255)
                                                                                                                       aGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTa                         <  1:137513/148‑1 (MQ=255)
                                                                                                                       aGCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTa                         >  1:1953614/1‑148 (MQ=255)
                                                                                                                        gCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTAt                        >  1:1031067/1‑148 (MQ=255)
                                                                                                                        gCAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTAt                        <  2:2041556/148‑1 (MQ=255)
                                                                                                                         cAACGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATg                       >  2:3231936/1‑148 (MQ=255)
                                                                                                                           aCGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGtt                     >  2:2275422/1‑148 (MQ=255)
                                                                                                                            cGTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGttt                    <  2:2183880/148‑1 (MQ=255)
                                                                                                                             gTAGAGTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTtg                   <  2:3162633/148‑1 (MQ=255)
                                                                                                                                gagTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgc                <  1:1093200/148‑1 (MQ=255)
                                                                                                                                 agTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgcg               >  1:3438476/1‑148 (MQ=255)
                                                                                                                                 agTTGGTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTgtgcg               >  2:1652248/1‑148 (MQ=255)
                                                                                                                                     ggTCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCa           <  1:1650569/148‑1 (MQ=255)
                                                                                                                                       tCATGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACg         >  2:2041796/1‑148 (MQ=255)
                                                                                                                                         aTGGAAAAAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCg                             <  1:2270463/126‑1 (MQ=255)
                                                                                                                                             aaaaAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATtga   >  1:155473/1‑148 (MQ=255)
                                                                                                                                             aaaaAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATtga   <  1:3231710/148‑1 (MQ=255)
                                                                                                                                             aaaaAGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATtga   >  1:2334146/1‑148 (MQ=255)
                                                                                                                                              aaaaGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATtgat  >  1:2433499/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGTTGGTCATGGAAAACGGTCAAACCTATCCCCTGAAAGGTACGCTGCAATTCTCCGATGTGACCGTTGATGAAAGCACCGGCTCCATAACCCTACGTGCTGTCTTCCCTAACCCGCAACATACGCTTTTGCCGGGTATGTTTGTGCGTGCACGGATTGAT  >  CP009273/3407824‑3408113

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑