Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,439,443 G→T 8.2% I19I (ATC→ATA)  rplF ← 50S ribosomal subunit protein L6

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,439,4430GT 8.2% 540.5 / 2.8 210I19I (ATC→ATA) rplF50S ribosomal subunit protein L6
Reads supporting (aligned to +/- strand):  ref base G (83/99);  new base T (8/20);  total (91/119)
Fisher's exact test for biased strand distribution p-value = 1.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 212 total aligned reads displayed.

CGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAGGT  >  CP009273/3439296‑3439589
                                                                                                                                                   |                                                                                                                                                  
cgcgGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTg                                                                                                                                                    <  1:2924624/148‑1 (MQ=255)
cgcgGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTg                                                                                                                                                    <  2:2862700/148‑1 (MQ=255)
  cgGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGAt                                                                                                                                                  <  2:1587124/148‑1 (MQ=255)
   ggTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGAtt                                                                                                                                                 <  2:2071883/148‑1 (MQ=255)
          gCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTGTTTTCCTTTGATCGTAATAAACAGACCGTTGAttttttagt                                                                                                                                          >  1:2110256/1‑144 (MQ=255)
            cTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCa                                                                                                                                        <  1:1160933/148‑1 (MQ=255)
             tgtgCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCaa                                                                                                                                       <  1:2534094/148‑1 (MQ=255)
               tgCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACg                                                                                                                                     <  1:1096297/148‑1 (MQ=255)
                gCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGc                                                                                                                                    <  2:2573263/148‑1 (MQ=255)
                    aaCCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGc                                                                                                                                <  1:2970414/148‑1 (MQ=255)
                         tCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaa                                                                                                                           <  1:3083390/148‑1 (MQ=255)
                         tCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaa                                                                                                                           >  2:3397117/1‑148 (MQ=255)
                           tGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaaca                                                                                                                         <  1:3266628/148‑1 (MQ=255)
                            gCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaacaa                                                                                                                        <  1:1891863/148‑1 (MQ=255)
                            gCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaacaa                                                                                                                        <  1:2031792/148‑1 (MQ=255)
                            gCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaacaa                                                                                                                        <  1:446793/148‑1 (MQ=255)
                             cGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGaacaac                                                                                                                       <  1:1104527/148‑1 (MQ=255)
                               tAACCATCACGCGGACCGAAGGTCTGGTTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGa                                                                                                                     <  2:2957881/148‑1 (MQ=255)
                                aaCCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGAc                                                                                                                    >  2:192285/1‑148 (MQ=255)
                                aaCCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGAc                                                                                                                    >  2:388013/1‑148 (MQ=255)
                                 aCCATCACGCGGACCGAAGGTCTGGTTTTTATCTGCTTTTTTAACTTCAACAGCTTCTTTGATTTTACGAGTCAGCTCGCCGTTTTTACCTTTTATCGTAATAACCTTACGTTTTATTTTTACGTAAACGCCGGCAGGAACAACGAcc                                                                                                                   <  2:1339385/148‑1 (MQ=255)
                                  ccATCACGCGGACCGAAGGTCTGGGTATTATCGGCATGTTTAACTTCAACAGCATCTTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCACCGCCGGCAGGAACAACGACCg                                                                                                                  <  2:2638182/148‑1 (MQ=255)
                                     tCACGCGGACCGATGGTCAGGTTATTATCTGCATGTTTAACTTCAACAGCATCTTTGAGAGTACTATTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTAGGTCAAGGCCGGCAGGAACAACGACCGGTg                                                                                                               <  2:3433733/148‑1 (MQ=255)
                                          cgGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTa                                                                                                          >  2:1149289/1‑148 (MQ=255)
                                             aCCGACGGTCAGGGTATTATCTGCATGTTTAACTTCAACACCATCGTTGAGAGTACGAGTCAGCTCGCCTTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCa                                                                                                       <  2:156785/148‑1 (MQ=255)
                                             aCCGAAGGTCTGGGTTTTATCTGCATTTTTAACTTCAACCGCATCGTTGAGAGTACGAGTCTGCTCGCCGTTTTTACCTTTTATCGTAATAACCTGACCGTTGATTTTTACGACAACGCCGGCAGGAACAACGACCGGTGCTTTAGCa                                                                                                       <  1:2033988/148‑1 (MQ=255)
                                               cGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTAAGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAac                                                                                                     >  2:2472180/1‑148 (MQ=255)
                                                 aaGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCCACGCCGGCAGGAACAACGACCGGTGCTTTAGCAacac                                                                                                   >  2:1297741/1‑148 (MQ=255)
                                                   ggTCAGGGTCTTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACCACCACCGGTGCTTTAGCAACACga                                                                                                 >  2:2911486/1‑148 (MQ=255)
                                                   ggTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCGTTGATCGTAATAACCTGACCGTTGATTTTTAAGTCAACGCCGGCAGGACCCACGACCGGTGCTTTAGCAACACga                                                                                                 >  2:1658728/1‑148 (MQ=255)
                                                    gTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCGTTGATCGTAATAAACGGACCGTTGATTTTTAAGTCAACGCCGGCAGGCACCACGACCGGTGCGTTTGGAACACcag                                                                                                >  1:1610957/1‑148 (MQ=255)
                                                         ggTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACAtt                                                                                           <  2:3218005/148‑1 (MQ=255)
                                                         ggTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTGTTTAGCTTTGTTGGTAATAAACAGACCGTTGTTTTTTTAGTCAAACCCGGCCGGGAAAACGCCCGGGGCGGTAGGAACACCAGACaat                                                                                           >  1:913846/1‑146 (MQ=255)
                                                             ttatCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTGTTTACAATTTATAGTAATAAACTGAACCTTTATTTTTACGTCAACCCCCGCCCGCACAACGACCGCTGCGTTAGCAACAACAGACAattttt                                                                                       >  1:789242/1‑148 (MQ=255)
                                                              tatCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTGTTTTACTTTTTTCGTAATAAACGTACCGTTTATTTTTTAGTCAAAGCCCGCCGGAACAAAGACCCGTGCGTTTGCCACACCAGACAttttttt                                                                                      >  1:2672044/1‑147 (MQ=255)
                                                                tCTGCTTTTTTAACTACACCAGCTTGGTTGATAGTACGAGTCTGCTCGCCGTTTTTACATTTGATCTTAATACCCTGCCGTTTGATTTTACCGTCAACGCCGGCCGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTtcct                                                                                    <  1:1361616/148‑1 (MQ=255)
                                                                  tGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACCACGACCGGTGCTTTAGCAACACGAGACATTTTTtcctcc                                                                                  >  2:3110793/1‑148 (MQ=255)
                                                                      tGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTTATTTTTAAGTCAACGCCGGCAGGAAAAAAGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGAtt                                                                              >  2:1093787/1‑148 (MQ=255)
                                                                       ttttAACTACAACAGATTCTTTGATAGTACGAGTCAGCTCGCGGTTTTTACATTTTATCTTAATAACCTGACGGTTTATTTTTACGTCACCGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTa                                                                             <  1:1864605/147‑1 (MQ=255)
                                                                       gTTTAACTTCAACCGCATGGTTGATATTACGAGACAGCTCGCGGTTTTTAACTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACCACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTa                                                                             <  2:137598/148‑1 (MQ=255)
                                                                         ttAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTCCGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAgg                                                                           <  2:2937339/148‑1 (MQ=255)
                                                                            aCTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTa                                                                        <  2:3227669/148‑1 (MQ=255)
                                                                             cTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTAc                                                                       >  2:890702/1‑148 (MQ=255)
                                                                             cTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAAACAGACCGTTGTTTTTTAAGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGTTTAGGCTAc                                                                       >  1:1744865/1‑148 (MQ=255)
                                                                                 aaCAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAg                                                                   <  2:1766715/148‑1 (MQ=255)
                                                                                   cAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCCGTGCTTTAGCAACACGAGACAATTTTTACTCCGATTAGGCTACGTAGCa                                                                 >  2:2681208/1‑148 (MQ=255)
                                                                                   cAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTGTTTAACTTTTATCGTAATAACCAGACCGTTTATTTTTAAGTCAACGCCCGCAGGAACAACGACCGGTGCTTTATCAACACGAGACATTTTTTCCTCCGATTAGGCGACGTAGCa                                                                 >  1:825087/1‑148 (MQ=255)
                                                                                    aGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAg                                                                <  2:1821012/148‑1 (MQ=255)
                                                                                     gCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAAAAACCAGACCGTTGATTTTTACGTCAACCCCCGCAGGAACAACGACCCGTGCTTTAGCAACACGAGACATTTTTTCCTCCGCTTATGCTAAGTAGCAGa                                                               >  1:242259/1‑148 (MQ=255)
                                                                                        tCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATaa                                                            >  2:2146209/1‑148 (MQ=255)
                                                                                         cGTTGAGTGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAAt                                                           <  2:794870/148‑1 (MQ=255)
                                                                                         cGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACCCCCGCAGGAACAACCACCGGTGCTTTAGCAAAACGAGACATTTTTTACTCCGAATAGGCTACGTAGCAGATAAt                                                           >  2:335610/1‑148 (MQ=255)
                                                                                          gTTGAGAGTACGCTTCAGCTCGCGTTTTTTACCTTTGATCGTAATAACCTGACCTTTGATTTTTCCGACACCGCCGGCCGCAACCACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAAtt                                                          <  1:1623795/148‑1 (MQ=255)
                                                                                          gTTGAGAGTACGAGACAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGACCAACGACCGGTGCTTTAGCAACACGAGACATTTTGTCCTCCGATTAGGCTACGTAGCAGATAAtt                                                          <  2:1990539/148‑1 (MQ=255)
                                                                                           ttGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCGTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCGACGTAGCAGATAAttt                                                         >  2:155048/1‑148 (MQ=255)
                                                                                            tGAGAGTACGAGTCAGCTCGCCGTTTTTACCATTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCCGCAGGAACAACGACCGGTGCTTTAGCACCACGACACAATTTTTCCACCGATTAGGCTACGTAGCAGATAATTTc                                                        >  2:653582/1‑148 (MQ=255)
                                                                                              agagTACGAGTCAGCTGGCGTTTTTTACTTTTTTTCGTAATAACCTGACGTTTGTTTTTTACGTCAACGCCGGCAGTAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGc                                                      <  1:2206975/148‑1 (MQ=255)
                                                                                              agagTACGAGTCAGCACGGCGTTTTTACCTTGGATCTTAATAACCTGACCGTTGATTTTCACGGCACCGCCGGCCGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGc                                                      <  2:3271117/148‑1 (MQ=255)
                                                                                                agTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGGAACGTAGCAGCTAATTTCGcca                                                    >  2:1842544/1‑148 (MQ=255)
                                                                                                     gAGTCAGCTCGCCGTTTTTACCTTTTATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAg                                               <  2:1219666/148‑1 (MQ=255)
                                                                                                      aGTCAGCTCGCCGTTTTTACATTTTATCGTAATAACCTGACCTTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGa                                              <  1:2501757/148‑1 (MQ=255)
                                                                                                          aGCTCGCTGTTTTTACATTTTATCTTAAAACCCTGCCGTTTTTTTTTTCCGACACCGCCGGCCGCAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAg                                          <  1:2944558/148‑1 (MQ=255)
                                                                                                           gCTCGCCTTTTTTACCTTTGATCGTAATAACCTGACCTTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGc                                         <  2:789242/148‑1 (MQ=255)
                                                                                                           gCTCGCCTTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTCCGTCAACGCCGGCCGTAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGc                                         <  1:2170309/148‑1 (MQ=255)
                                                                                                            cTCGCCGTTTTTACCTTTGATCGTAATACCCTTACCGTTGATTTTTCCGACAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTGTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGcc                                        <  2:2358676/148‑1 (MQ=255)
                                                                                                               gCCGTTTTTACCTTTGATCGTAATAACCTGACAGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTgg                                     <  2:1954083/148‑1 (MQ=255)
                                                                                                                   tttttACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATCATTTCGCCACCAAGACCAGCCTGgcgcg                                 >  2:1041737/1‑148 (MQ=255)
                                                                                                                   tttttACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGgcgcg                                 <  1:2298885/148‑1 (MQ=255)
                                                                                                                     tttACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCt                               >  1:2987339/1‑148 (MQ=255)
                                                                                                                     tttACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCt                               >  2:2233732/1‑148 (MQ=255)
                                                                                                                        aCCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGACCAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCa                            <  2:3340179/148‑1 (MQ=255)
                                                                                                                         ccTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTCCGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCAc                           >  2:1242208/1‑148 (MQ=255)
                                                                                                                         ccTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCAc                           >  1:34945/1‑148 (MQ=255)
                                                                                                                          cTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACg                          >  2:2550798/1‑148 (MQ=255)
                                                                                                                          cTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACg                          >  2:1336289/1‑148 (MQ=255)
                                                                                                                           tttGATCGTAATAACCTGACCGTTGATTTGAACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGa                         <  2:3222164/148‑1 (MQ=255)
                                                                                                                            ttGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGAt                        <  2:1923086/148‑1 (MQ=255)
                                                                                                                              gATCGTAATAACCTGACCGTTTATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCa                      <  2:2672044/148‑1 (MQ=255)
                                                                                                                              gATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCa                      <  2:922913/148‑1 (MQ=255)
                                                                                                                              gATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCa                      <  2:2295439/148‑1 (MQ=255)
                                                                                                                              gATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCa                      <  1:3397117/148‑1 (MQ=255)
                                                                                                                              gATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCa                      <  1:3502624/148‑1 (MQ=255)
                                                                                                                              gATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCa                      >  1:1961994/1‑148 (MQ=255)
                                                                                                                              gATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCa                      <  1:1766433/148‑1 (MQ=255)
                                                                                                                                 cGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTc                   >  1:3190194/1‑148 (MQ=255)
                                                                                                                                 cGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTc                   <  2:1434192/148‑1 (MQ=255)
                                                                                                                                 cGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTc                   <  1:811425/148‑1 (MQ=255)
                                                                                                                                 cGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTc                   >  2:2773731/1‑148 (MQ=255)
                                                                                                                                     ataaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATaa               >  1:1451162/1‑148 (MQ=255)
                                                                                                                                      taaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAac              >  2:2378291/1‑148 (MQ=255)
                                                                                                                                      taaCCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAac              >  1:584907/1‑148 (MQ=255)
                                                                                                                                         ccTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACAcc           >  1:3124858/1‑148 (MQ=255)
                                                                                                                                          cTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCt          >  1:926985/1‑148 (MQ=255)
                                                                                                                                            gACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCttt        >  2:411117/1‑148 (MQ=255)
                                                                                                                                             aCCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTa       >  1:252049/1‑148 (MQ=255)
                                                                                                                                               cGTTTATTTAGACTTCAACGCCGGCAGTAACCACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTaga     <  1:2367432/148‑1 (MQ=255)
                                                                                                                                               cGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTaga     <  2:825087/148‑1 (MQ=255)
                                                                                                                                                 ttGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAgg   >  1:2286004/1‑148 (MQ=255)
                                                                                                                                                 ttGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAgg   >  2:1249155/1‑148 (MQ=255)
                                                                                                                                                  tGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAGGt  >  1:2752468/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGCGGTACCAGCCTGTGCCCAACCGTCTGCGTAACCATCACGCGGACCGAAGGTCAGGGTATTATCTGCATGTTTAACTTCAACAGCATCGTTGAGAGTACGAGTCAGCTCGCCGTTTTTACCTTTGATCGTAATAACCTGACCGTTGATTTTTACGTCAACGCCGGCAGGAACAACGACCGGTGCTTTAGCAACACGAGACATTTTTTCCTCCGATTAGGCTACGTAGCAGATAATTTCGCCACCAAGACCAGCCTGGCGCGCTGCACGATCAGTCATAACACCTTTAGAGGT  >  CP009273/3439296‑3439589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑