Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,440,832 T→C 100% N99D (AAC→GAC)  rplX ← 50S ribosomal subunit protein L24

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,440,8320TC100.0% 1031.8 / NA 249N99D (AAC→GAC) rplX50S ribosomal subunit protein L24
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (142/107);  total (142/107)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

GTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGG  >  CP009273/3440687‑3440975
                                                                                                                                                 |                                                                                                                                               
gTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAg                                                                                                                                               <  1:1241489/148‑1 (MQ=255)
      ggTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTga                                                                                                                                         >  2:1875972/1‑148 (MQ=255)
       gTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTgaa                                                                                                                                        >  1:3313819/1‑148 (MQ=255)
          aTCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTgaagaa                                                                                                                                     <  2:1370007/148‑1 (MQ=255)
          aTCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTgaagaa                                                                                                                                     >  1:261995/1‑148 (MQ=255)
          aTCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTgaagaa                                                                                                                                     <  1:2068134/148‑1 (MQ=255)
            cttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAAc                                                                                                                                   >  2:3352427/1‑148 (MQ=255)
             ttctCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACg                                                                                                                                  <  2:2992682/148‑1 (MQ=255)
               ctcGACCCGAGGGACGTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGa                                                                                                                                <  1:2122254/148‑1 (MQ=255)
                  gACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtt                                                                                                                             <  1:853118/148‑1 (MQ=255)
                    cccGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttt                                                                                                                           <  1:2260564/148‑1 (MQ=255)
                    cccGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttt                                                                                                                           <  2:311389/148‑1 (MQ=255)
                      cGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACtttttt                                                                                                                         <  2:880012/148‑1 (MQ=255)
                       gAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTa                                                                                                                        <  1:1664016/148‑1 (MQ=255)
                         gggACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTCTTTTAcc                                                                                                                      >  2:1278893/1‑148 (MQ=255)
                           gACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGt                                                                                                                    <  2:632574/148‑1 (MQ=255)
                           gACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGt                                                                                                                    <  2:2781443/148‑1 (MQ=255)
                             cTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCt                                                                                                                  <  1:77495/148‑1 (MQ=255)
                             cTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCt                                                                                                                  >  2:2790332/1‑148 (MQ=255)
                              ttGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCtt                                                                                                                 <  1:1269246/148‑1 (MQ=255)
                               tGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTACTCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGTAGAAACGGACTTTTTTACCGTCTTc                                                                                                                >  1:1212804/1‑148 (MQ=255)
                               tGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTc                                                                                                                >  2:1604314/1‑148 (MQ=255)
                               tGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTc                                                                                                                <  1:1231198/148‑1 (MQ=255)
                                 cATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGa                                                                                                              <  2:916603/148‑1 (MQ=255)
                                 cATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGa                                                                                                              <  2:1807878/148‑1 (MQ=255)
                                 cATGACAGAATTGTAGTTAAACTCACTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGa                                                                                                              <  2:2669071/148‑1 (MQ=255)
                                  aTGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGaa                                                                                                             <  1:422958/148‑1 (MQ=255)
                                   tGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAAt                                                                                                            <  2:347209/148‑1 (MQ=255)
                                    gACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATc                                                                                                           >  1:3435545/1‑148 (MQ=255)
                                    gACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATc                                                                                                           >  1:2973547/1‑148 (MQ=255)
                                        gAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTaaa                                                                                                       >  1:3945/1‑148 (MQ=255)
                                         caTTGTAGTTAAACTCAGTCATGAGCTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAg                                                                                                      <  2:108586/147‑1 (MQ=255)
                                           ttGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGcc                                                                                                    >  1:2451054/1‑148 (MQ=255)
                                           ttGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGcc                                                                                                    >  1:212492/1‑148 (MQ=255)
                                           ttGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGcc                                                                                                    <  1:877531/148‑1 (MQ=255)
                                           ttGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGcc                                                                                                    >  1:1986904/1‑148 (MQ=255)
                                              tAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTac                                                                                                 >  1:2801057/1‑148 (MQ=255)
                                                 ttAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTa                                                                                                                        <  1:2690569/122‑1 (MQ=255)
                                                   aaaCTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGt                                                                                            >  1:1464124/1‑148 (MQ=255)
                                                      cTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAg                                                                                         >  2:552260/1‑148 (MQ=255)
                                                      cTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAg                                                                                         >  2:2155506/1‑148 (MQ=255)
                                                       tCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGc                                                                                        <  1:1724805/148‑1 (MQ=255)
                                                       tCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGc                                                                                        <  1:1096847/148‑1 (MQ=255)
                                                       tCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGc                                                                                        <  1:1367156/148‑1 (MQ=255)
                                                         aGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCt                                                                                      >  1:2400046/1‑148 (MQ=255)
                                                           tCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                    >  2:2255846/1‑148 (MQ=255)
                                                           tCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                    >  1:967594/1‑148 (MQ=255)
                                                           tCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                    >  1:1811266/1‑148 (MQ=255)
                                                           tCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                    >  1:2821523/1‑148 (MQ=255)
                                                           tCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTg                                                                                    >  2:2842373/1‑148 (MQ=255)
                                                            cATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGc                                                                                   >  1:1317601/1‑148 (MQ=255)
                                                            cATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGc                                                                                   <  2:322396/148‑1 (MQ=255)
                                                              tGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCg                                                                                 >  2:808117/1‑148 (MQ=255)
                                                              tGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCg                                                                                 >  2:1200201/1‑148 (MQ=255)
                                                                aGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGt                                                                               <  2:151788/148‑1 (MQ=255)
                                                                aGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGt                                                                               <  1:1091853/148‑1 (MQ=255)
                                                                 gTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGtt                                                                              >  2:657656/1‑148 (MQ=255)
                                                                     tttAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCg                                                                          >  2:1661838/1‑148 (MQ=255)
                                                                         actactTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCAtt                                                                      >  2:2986010/1‑148 (MQ=255)
                                                                            actTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGaa                                                                   <  2:932389/148‑1 (MQ=255)
                                                                            actTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGaa                                                                   <  2:261995/148‑1 (MQ=255)
                                                                               tCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAt                                                                <  1:45475/148‑1 (MQ=255)
                                                                                cGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGAtt                                                               >  2:3190726/1‑148 (MQ=255)
                                                                                  tCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGc                                                             <  1:1467180/148‑1 (MQ=255)
                                                                                   cTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCt                                                            <  1:105701/148‑1 (MQ=255)
                                                                                    tttGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTa                                                           >  2:2739534/1‑148 (MQ=255)
                                                                                      tGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACg                                                         <  1:2083625/148‑1 (MQ=255)
                                                                                      tGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACg                                                         <  2:3372036/148‑1 (MQ=255)
                                                                                         agtaATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTg                                                      >  1:1005970/1‑148 (MQ=255)
                                                                                           taATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGa                                                    >  2:213187/1‑148 (MQ=255)
                                                                                             aTCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGaaa                                                  <  1:645352/148‑1 (MQ=255)
                                                                                              tCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAAc                                                 <  2:1983186/148‑1 (MQ=255)
                                                                                              tCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAAc                                                 >  2:2103118/1‑148 (MQ=255)
                                                                                              tCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAAc                                                 <  1:519766/148‑1 (MQ=255)
                                                                                              tCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAAc                                                 <  1:164088/148‑1 (MQ=255)
                                                                                                 tGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTg                                              >  1:985991/1‑148 (MQ=255)
                                                                                                  gCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGa                                             >  2:314384/1‑148 (MQ=255)
                                                                                                   cAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGaa                                            <  2:1462872/148‑1 (MQ=255)
                                                                                                    aGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAAt                                           >  2:134488/1‑148 (MQ=255)
                                                                                                     gTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATa                                          <  2:2693564/148‑1 (MQ=255)
                                                                                                           ccATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGct                                    >  2:1422843/1‑148 (MQ=255)
                                                                                                           ccATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGct                                    >  2:1033939/1‑148 (MQ=255)
                                                                                                             aTCGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGcttc                                  <  2:170066/148‑1 (MQ=255)
                                                                                                               cGTACTACTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGcttctt                                >  1:2892003/1‑148 (MQ=255)
                                                                                                                 tactacTCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCtttt                              >  2:883345/1‑148 (MQ=255)
                                                                                                                     actCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCaa                          <  1:1941756/148‑1 (MQ=255)
                                                                                                                     actCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCaa                          <  2:2056691/148‑1 (MQ=255)
                                                                                                                     actCCAAATTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCaa                          >  1:1025777/1‑148 (MQ=255)
                                                                                                                            aTTACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGcc                   >  1:2245482/1‑148 (MQ=255)
                                                                                                                              tACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCAc                 >  1:3121942/1‑148 (MQ=255)
                                                                                                                              tACTTGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCAc                 >  1:3121997/1‑148 (MQ=255)
                                                                                                                                 ttGATAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCg              >  2:1904744/1‑148 (MQ=255)
                                                                                                                                    aTAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggtt           >  2:2417718/1‑148 (MQ=255)
                                                                                                                                    aTAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggtt           >  2:2417638/1‑148 (MQ=255)
                                                                                                                                     tAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttg          >  1:3427815/1‑148 (MQ=255)
                                                                                                                                     tAGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttg          >  2:3445747/1‑148 (MQ=255)
                                                                                                                                      aGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCggttgg         >  1:349698/1‑148 (MQ=255)
                                                                                                                                      aGTTTCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTAAACGATGCCACCCggttgg         >  1:349650/1‑148 (MQ=255)
                                                                                                                                         ttCGCTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTc      >  1:3205941/1‑148 (MQ=255)
                                                                                                                                             cTGTCAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAggg  <  2:2937790/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
GTTCAGGGTGATCTTCTCGACCCGAGGGACTTGCATGACAGAATTGTAGTTAAACTCAGTCATGAGTTTTTTAACTACTTCGTCTTTGTAGTAATCATGCAGTTTCGCCATCGTACTACTCCAAATTACTTGATAGTTTCGCTGTTAGACTTGAAGAAACGGACTTTTTTACCGTCTTCGAATCTAAAGCCTACACGGTCAGCCTTGCCGGTTGCCGCATTGAAGATTGCTACGTTGGAAACCTGAATAGCGGCTTCTTTTTCAACGATGCCACCCGGTTGGTTCAGGG  >  CP009273/3440687‑3440975

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑