Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,509,102 G→T 100% S57R (AGC→AGA)  dam ← DNA adenine methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,509,1020GT98.4% 601.6 / ‑0.6 190S57R (AGC→AGA) damDNA adenine methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (2/1);  new base T (98/89);  total (100/90)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 193 total aligned reads displayed.

CGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAC  >  CP009273/3508956‑3509246
                                                                                                                                                  |                                                                                                                                                
 ggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTctct                                                                                                                                                <  1:2199497/148‑1 (MQ=255)
 ggaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTctct                                                                                                                                                <  1:3224658/148‑1 (MQ=255)
  gaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTa                                                                                                                                               <  1:2910308/148‑1 (MQ=255)
  gaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTa                                                                                                                                               >  1:3111290/1‑148 (MQ=255)
  gaacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTa                                                                                                                                               >  2:1599122/1‑148 (MQ=255)
   aacggaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTAt                                                                                                                                              <  2:3348753/148‑1 (MQ=255)
       gaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGat                                                                                                                                          <  1:1969295/148‑1 (MQ=255)
       gaTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGat                                                                                                                                          >  2:1986936/1‑148 (MQ=255)
        aTCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGata                                                                                                                                         <  2:3022370/148‑1 (MQ=255)
         tCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGatat                                                                                                                                        <  1:1596882/148‑1 (MQ=255)
            tGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCgg                                                                                                                                     <  2:3469397/148‑1 (MQ=255)
              gCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCa                                                                                                                                   >  1:3141718/1‑148 (MQ=255)
               cTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCaa                                                                                                                                  <  1:266462/148‑1 (MQ=255)
                ttttGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAg                                                                                                                                 >  2:1507210/1‑148 (MQ=255)
                ttttGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAg                                                                                                                                 >  2:1329892/1‑148 (MQ=255)
                 tttGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAgg                                                                                                                                <  2:2694811/148‑1 (MQ=255)
                 tttGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAgg                                                                                                                                <  1:1225384/148‑1 (MQ=255)
                  ttgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGa                                                                                                                               <  1:580315/148‑1 (MQ=255)
                   tgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGat                                                                                                                              <  1:1401813/148‑1 (MQ=255)
                   tgttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGat                                                                                                                              <  1:2240234/148‑1 (MQ=255)
                    gttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGata                                                                                                                             >  2:1831331/1‑148 (MQ=255)
                    gttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGata                                                                                                                             <  2:2095977/148‑1 (MQ=255)
                    gttgAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTGTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGata                                                                                                                             <  2:3081750/148‑1 (MQ=255)
                         aCTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACg                                                                                                                        <  2:1127029/148‑1 (MQ=255)
                          ctctTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACga                                                                                                                       >  1:107252/1‑148 (MQ=255)
                           tctTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACgag                                                                                                                      >  2:582461/1‑148 (MQ=255)
                              tCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGaaa                                                                                                                   >  1:1915668/1‑148 (MQ=255)
                                 ctgAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGAGATAACGAGAAAAGt                                                                                                                >  2:1789250/3‑148 (MQ=255)
                                   gAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCg                                                                                                              <  2:1232745/148‑1 (MQ=255)
                                    aaCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATCACGAGAAAAGTCgg                                                                                                             >  2:909458/1‑148 (MQ=255)
                                    aaCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCgg                                                                                                             >  2:861901/1‑148 (MQ=255)
                                     aCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgt                                                                                                            <  1:2243449/148‑1 (MQ=255)
                                     aCTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgt                                                                                                            <  1:475261/148‑1 (MQ=255)
                                      cTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtg                                                                                                           >  1:2240555/1‑148 (MQ=255)
                                       tGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtgt                                                                                                          >  1:2032971/1‑148 (MQ=255)
                                       tGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGgtgt                                                                                                          <  1:3286148/148‑1 (MQ=255)
                                         aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTg                                                                                                        >  1:2425290/1‑148 (MQ=255)
                                         aTAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTg                                                                                                        <  2:1583515/148‑1 (MQ=255)
                                            gTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTgaga                                                                                                     <  1:139086/148‑1 (MQ=255)
                                             tAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaa                                                                                                    <  1:2683779/148‑1 (MQ=255)
                                             tAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaa                                                                                                    <  1:2683831/148‑1 (MQ=255)
                                             tAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaa                                                                                                    >  1:2467022/1‑148 (MQ=255)
                                             tAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaa                                                                                                    >  1:432898/1‑148 (MQ=255)
                                              aaaCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGaaa                                                                                                   >  2:1723090/1‑148 (MQ=255)
                                               aaCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAac                                                                                                  >  1:2701921/1‑148 (MQ=255)
                                                aCCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAaca                                                                                                 >  2:2023148/1‑148 (MQ=255)
                                                     ggCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAc                                                                                            <  2:698347/148‑1 (MQ=255)
                                                       cgcAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGAccc                                                                                          >  1:798894/1‑148 (MQ=255)
                                                         cAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCgg                                                                                        >  1:192107/1‑148 (MQ=255)
                                                         cAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCgg                                                                                        >  2:1620915/1‑148 (MQ=255)
                                                           aTTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGGTCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCa                                                                                      >  1:1125036/1‑148 (MQ=255)
                                                           aTTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCa                                                                                      >  1:393867/1‑148 (MQ=255)
                                                            tttGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAc                                                                                     >  2:493532/1‑148 (MQ=255)
                                                            tttGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCAc                                                                                     >  2:350252/1‑148 (MQ=255)
                                                                 ttCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACa                                                                                >  2:2262058/1‑148 (MQ=255)
                                                                      taacaaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAAGTTTATAGAGACTGATCAGGGCTCTATTGATATCGGCAAGGATATACCGAGAAAAGTCGGTGTTGAGAAACCCCGACCCGGCACCTACAAAAgg                                                                           <  2:813730/147‑1 (MQ=255)
                                                                      gAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAgg                                                                           >  2:1283985/1‑148 (MQ=255)
                                                                      gAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAgg                                                                           <  2:280349/148‑1 (MQ=255)
                                                                       aacaaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGc                                                                          <  2:2580473/148‑1 (MQ=255)
                                                                        acaaaccGCGGGGGTGGGGCGTGTCCGTACTCATCAGTCCGCATCTCCAAAATGTTATTGAGACTGACCTGGTATCTTTTGATATCGCAACGTATATAACGATAAAAGTGGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCt                                                                         <  2:1489087/148‑1 (MQ=255)
                                                                          aaacaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGATAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCa                                                                       >  1:1395623/1‑148 (MQ=255)
                                                                            acaGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAAc                                                                     <  2:3193573/148‑1 (MQ=255)
                                                                               gCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAg                                                                  >  2:2400547/1‑148 (MQ=255)
                                                                                cTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGa                                                                 >  1:3342444/1‑148 (MQ=255)
                                                                                cTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGa                                                                 >  1:1491493/1‑148 (MQ=255)
                                                                                  ggcgTGGGGCTTGTACTTACTCATCAGTACGATTTTTCACATTTTTTTATAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGATAAAAGTCGGTGTTGATAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACa                                                               <  2:3165055/147‑1 (MQ=255)
                                                                                       gCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGGGGTGAGAAACACCGACCCGGCACCTACCAATGGCTCAACCCGACAGTCGc                                                          >  2:2206255/1‑148 (MQ=255)
                                                                                        cGGCCTGTACGTACTAATCCGTACGCATCTTCACACTGTTATAGAGACGGATCAGGTCTCTTTTGATATCGGCAAGTATATAACTCGAAAAGGCGGGGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGcc                                                         <  2:2366283/148‑1 (MQ=255)
                                                                                             tGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTgg                                                    >  1:1214678/1‑148 (MQ=255)
                                                                                              gtacgtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTggg                                                   >  2:2311254/1‑148 (MQ=255)
                                                                                                 cgtacTCATCTGTACGCATATTCAAATTTTTATAGAGACTGATCTGGTATATTTTGATATCGGCAAGTATATAACTAGAAATGTGGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaa                                                <  1:1458142/148‑1 (MQ=255)
                                                                                                  gtacTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCaaa                                               <  2:1424275/148‑1 (MQ=255)
                                                                                                    acTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg                                             <  2:1915668/148‑1 (MQ=255)
                                                                                                    acTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATg                                             <  2:2874807/148‑1 (MQ=255)
                                                                                                    acTCATCAGTACGCATCTTCACAATGTTATAGAGACTGAACAGGTCTCTATTGATATCGGCAATGATATAACGAGAAAACTCGGTGATGAGAAACACCGAACCGCCACCTACAAAAGGCTCAACCAGACATTAGCCCTAGGTCAAATg                                             >  1:319722/1‑148 (MQ=255)
                                                                                                       catcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCg                                          <  2:3218412/148‑1 (MQ=255)
                                                                                                         tcaGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGtt                                        >  1:3051866/1‑148 (MQ=255)
                                                                                                          caGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGttt                                       >  2:3074606/1‑148 (MQ=255)
                                                                                                          caGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGttt                                       >  1:1833874/1‑148 (MQ=255)
                                                                                                             aaCGCATCTCCACATTGTTATCGACAGTGATCTGGTATTTTTTTATATCGGCAAGTATATACCGACAAATGGGGGTGTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAat                                    <  1:1299343/147‑1 (MQ=255)
                                                                                                              aCGCATCTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAata                                   >  2:1013979/1‑148 (MQ=255)
                                                                                                                    cTTCACAATGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCTCCCTTGGGCAAATGCCGTTTAAAAtcatca                             >  1:1683785/1‑148 (MQ=255)
                                                                                                                          actGTTATAGAGACTGATCAGGTCTATTTTTAAAGCGGCAAGGTTATAACGACAAAAGTCGGTTTTGACAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgg                       <  1:2246375/146‑1 (MQ=255)
                                                                                                                          aaTGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgg                       >  2:1920505/1‑148 (MQ=255)
                                                                                                                          aaTGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAgg                       >  2:454922/1‑148 (MQ=255)
                                                                                                                           ttGTTATAGTCACTGATCAGGTCTATATTGATATCGCCACGTATATACCGATAAACGTGGTTGTTTACAAACCCCCACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                      <  1:1020874/147‑1 (MQ=255)
                                                                                                                           aTGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAggg                      >  2:3049100/1‑148 (MQ=255)
                                                                                                                           aTGTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCATTGGGCAAATGCCGTTTAATATCATCAAGCAggg                      >  1:407502/1‑148 (MQ=255)
                                                                                                                             gTTATAGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGa                    >  1:3083474/1‑148 (MQ=255)
                                                                                                                                 taGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACt                >  2:419279/1‑148 (MQ=255)
                                                                                                                                 taGAGACTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACt                >  1:1896840/1‑148 (MQ=255)
                                                                                                                                   gagaCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTg              >  2:2105552/1‑148 (MQ=255)
                                                                                                                                    agaCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGc             >  2:717386/1‑148 (MQ=255)
                                                                                                                                    agaCTGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGc             >  2:2804896/1‑148 (MQ=255)
                                                                                                                                       cttaTCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGcccc          <  2:1538055/145‑1 (MQ=255)
                                                                                                                                        tGATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTAGCCCATGGGCAAATGCCGTTTAATATCATCAAGACGGGGATACTTcccccc         >  2:1091582/1‑148 (MQ=255)
                                                                                                                                         gATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCt        >  1:88069/1‑148 (MQ=255)
                                                                                                                                         gATCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCt        >  2:2624969/1‑148 (MQ=255)
                                                                                                                                          aTCAGGTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTg       >  1:348248/1‑148 (MQ=255)
                                                                                                                                               gTCTCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAc  >  1:2343875/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CGGAACGGATCCTGGCTTTTGTTGAACTCTTCGCGGAACTGATAGTAAACCTCGGCGCAATTTGTTTCGGGAACAAACAGCTCGCGTGCGGCCTGTACGTACTCATCAGTACGCATCTTCACAATGTTATAGAGACTGATCAGGTCGCTATTGATATCGGCAAGGATATAACGAGAAAAGTCGGTGTTGAGAAACACCGACCCGGCACCTACAAAAGGCTCAACCAGACATTCGCCCTTGGGCAAATGCCGTTTAATATCATCAAGCAGGGGATACTTGCCCCCTGCCCAC  >  CP009273/3508956‑3509246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑