Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,535,370 C→A 100% S535R (AGC→AGA)  feoB → fused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,535,3700CA97.5% 508.1 / ‑4.7 181S535R (AGC→AGA) feoBfused ferrous iron transporter, protein B: GTP‑binding protein/membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/2);  major base A (107/61);  minor base T (0/11);  total (107/74)
Fisher's exact test for biased strand distribution p-value = 2.73e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 182 total aligned reads displayed.

TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTA  >  CP009273/3535225‑3535517
                                                                                                                                                 |                                                                                                                                                   
tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAgg                                                                                                                                                   <  1:2743084/148‑1 (MQ=255)
tGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAgg                                                                                                                                                   <  1:2326235/148‑1 (MQ=255)
 ggAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAggg                                                                                                                                                  >  2:943729/1‑148 (MQ=255)
 ggAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAggg                                                                                                                                                  >  1:383481/1‑148 (MQ=255)
 ggAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAggg                                                                                                                                                  <  1:1632495/148‑1 (MQ=255)
  gAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGa                                                                                                                                                 >  1:1023803/1‑148 (MQ=255)
  gAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGa                                                                                                                                                 >  1:3077934/1‑148 (MQ=255)
  gAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGa                                                                                                                                                 <  1:2727411/148‑1 (MQ=255)
  gAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGa                                                                                                                                                 >  1:1023742/1‑148 (MQ=255)
    gTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaa                                                                                                                                               <  1:1594077/148‑1 (MQ=255)
    gTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGaaa                                                                                                                                               <  1:2186475/148‑1 (MQ=255)
       gCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtc                                                                                                                                            <  2:1660827/148‑1 (MQ=255)
        ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcg                                                                                                                                           <  1:2792810/148‑1 (MQ=255)
        ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcg                                                                                                                                           <  2:1498310/148‑1 (MQ=255)
        ccGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAAtcg                                                                                                                                           <  2:1498392/148‑1 (MQ=255)
            tCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGa                                                                                                                                       <  1:3360644/148‑1 (MQ=255)
              tATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATa                                                                                                                                     >  2:348742/1‑148 (MQ=255)
              tATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATa                                                                                                                                     <  2:2088070/148‑1 (MQ=255)
                 cATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACa                                                                                                                                  >  2:2971997/1‑148 (MQ=255)
                   tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATc                                                                                                                                >  1:99589/1‑148 (MQ=255)
                   tGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATc                                                                                                                                >  2:1306353/1‑148 (MQ=255)
                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                               >  1:1272440/1‑148 (MQ=255)
                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                               >  1:1477236/1‑148 (MQ=255)
                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                               >  1:1477237/1‑148 (MQ=255)
                    gTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCa                                                                                                                               <  1:2643452/148‑1 (MQ=255)
                        cacaCGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGa                                                                                                                           <  2:801981/148‑1 (MQ=255)
                         acacGTTAAATGCCTTATTATCCAGACGTGACAGCGTCTTAATGGCTTCTTTCTGGGTGGTGTTAATGTGATAATAATGGTCAGATTTTTCGTGAGCGCTTTAAACTGCTTCTCGCTGTGAGGTAAAATCGTCGATAACATCAACGAc                                                                                                                          <  2:1628002/148‑1 (MQ=255)
                          cacGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACt                                                                                                                         >  2:1681766/1‑148 (MQ=255)
                           acGTTAAAGGCCTTATTATCCAGACCGGGCTGCGTCTGAAAGGATTCGTTCTGCGTGCTGGTAATGTGATATTCATCGTCAGATTTTTCCTGCGCGCTTTAAAATGATTATCGCTGTGTGGGAAAATCGTCTTTAACATCAACGACTc                                                                                                                        <  2:275801/148‑1 (MQ=255)
                               tAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGgcg                                                                                                                    >  2:2124302/1‑148 (MQ=255)
                                aaaaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGgcgc                                                                                                                   >  1:256827/1‑148 (MQ=255)
                                  aaGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTg                                                                                                                 >  1:3444736/1‑148 (MQ=255)
                                     ccTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcg                                                                                                              >  2:2614054/1‑148 (MQ=255)
                                        gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtcc                                                                                                           >  1:1940311/1‑148 (MQ=255)
                                        gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtcc                                                                                                           >  1:1939180/1‑148 (MQ=255)
                                        gATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTATCCTGAGCGCTTTCAACAGCTTCTCGATGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtcc                                                                                                           >  1:379130/1‑148 (MQ=255)
                                          ttATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGcgtccgt                                                                                                         >  2:2503321/1‑148 (MQ=255)
                                             tCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAg                                                                                                      >  2:3363416/1‑148 (MQ=255)
                                             tCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGAGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAg                                                                                                      >  2:2783088/1‑148 (MQ=255)
                                                 gACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCgg                                                                                                  >  1:162604/1‑148 (MQ=255)
                                                  aCCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCggg                                                                                                 >  1:2211160/1‑148 (MQ=255)
                                                   ccTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTAAACCGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGt                                                                                                <  2:266933/148‑1 (MQ=255)
                                                        cAGGGTCTTAATGGCTTGGTTCTGCGTGGTGTTAATGTGATCATCATCGCCCGCTTTTTCCTGAGCGCTTTCAACCGCTTCTCGCTGAGAGGTAAATTCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCa                                                                                           <  1:2807207/148‑1 (MQ=255)
                                                          gCGTCTGAACGGTTTGGTTCTGCGTGCTGGTAAAGTGATCATCCTCGTCAGAATTTTCCGGCGCGCTTTAAACAGCTTCTCGCTGTGAGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAcc                                                                                         <  1:2044124/148‑1 (MQ=255)
                                                           cGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCAccc                                                                                        >  1:1213987/1‑148 (MQ=255)
                                                              cTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCgg                                                                                     >  1:173088/1‑148 (MQ=255)
                                                                 aaaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCt                                                                                  <  2:2012346/148‑1 (MQ=255)
                                                                 aaaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCt                                                                                  >  2:3255747/1‑148 (MQ=255)
                                                                 aaaGGCTTCGTTCTCCGTGCTGGTAAAGTGATCATCAGCGTCTGCATTTTCCGGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCt                                                                                  <  2:339598/148‑1 (MQ=255)
                                                                  aaGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCtt                                                                                 >  1:654937/1‑148 (MQ=255)
                                                                    ggCTGCGGTCGGCGTGCTGGTAACGTGATCATCCTCGGCAGCATTTTCCTGCGCGCTTTCAACCGCTTCTCGCTGAGTGGTAAAATCGTCGATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCa                                                                               <  2:2374650/148‑1 (MQ=255)
                                                                      tttgtttCTGGGTGGTTTTAATGTTATAATCATCGTCATCTTTTTCCTGCGCGATTACAACAGCTTCTCGCTGAGTGGTAAAATCGTCTATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAg                                                                             <  1:3411669/143‑1 (MQ=255)
                                                                      cTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAg                                                                             >  1:2842899/1‑148 (MQ=255)
                                                                        tCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGcc                                                                           <  2:1343086/148‑1 (MQ=255)
                                                                        tCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCATCAAGcc                                                                           >  2:428806/1‑148 (MQ=255)
                                                                        tCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGATTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGcc                                                                           <  2:3322066/148‑1 (MQ=255)
                                                                         ctttCTGCGTGCTGTTAATGTTATCATCATGGTCAGATTTTTCCTGAGCGCTTACACCAGCTTCTCGCGGAGTGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCa                                                                          <  1:858358/146‑1 (MQ=255)
                                                                           ttCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAt                                                                        >  1:2503530/1‑148 (MQ=255)
                                                                            tCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAAtt                                                                       >  2:64197/1‑148 (MQ=255)
                                                                                   gCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                                >  2:1710966/1‑148 (MQ=255)
                                                                                   gCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                                >  1:1684829/1‑148 (MQ=255)
                                                                                   gCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGc                                                                >  1:1685146/1‑148 (MQ=255)
                                                                                     tGGTATAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTCCAACAGGTTCTCGCTTAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAt                                                              <  2:231172/148‑1 (MQ=255)
                                                                                      ggTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATg                                                             >  1:1868759/1‑148 (MQ=255)
                                                                                        tAAAGTGATCATCATCGTCAGAATTTTCCGGAGCGCTTTCAACAGCTTCTCGCTGCGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGaa                                                           <  2:1473781/148‑1 (MQ=255)
                                                                                         accttGATCATCATCGTAAGCATTTTCCTGAGCGATTGAACAAGCTTCTCGCTGAGAGGTAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAg                                                          <  2:2853232/144‑1 (MQ=255)
                                                                                          aaGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCAGGAAGa                                                         >  1:437782/1‑148 (MQ=255)
                                                                                           aGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGAt                                                        >  1:2480261/1‑148 (MQ=255)
                                                                                              gATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAAc                                                     >  2:2591704/1‑148 (MQ=255)
                                                                                                tcatcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTg                                                   >  1:2614482/1‑148 (MQ=255)
                                                                                                  atcatgTTCAGATTTTTCCTGCGGGTTTTCAACTGGTTCTCGCTTTGTGGTAAAATCGTCTATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                 <  1:2432447/148‑1 (MQ=255)
                                                                                                  atcatcGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                 <  2:3021569/148‑1 (MQ=255)
                                                                                                  atcagcGTCAGATTTTTCCTGAGCGCTTTCAACTGCTTCTCGCTGAGTGTAAAAATCGTCGAAAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggc                                                 <  1:2436996/148‑1 (MQ=255)
                                                                                                     atcGTCCGCATTTTCGGGTGCGATTCCAACCGCTTCTGGGTTAGTGGTAAAAGCGTCTTAAACACCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTggcagg                                              <  1:2989449/148‑1 (MQ=255)
                                                                                                         tCAGCATTTTCCTGCGCGCTTTCAACAGCTTCTCGCTGAGAGGCAAAATCGTCGATAACAACAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                          <  2:1560899/148‑1 (MQ=255)
                                                                                                         tCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCCATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                          >  2:3357249/1‑148 (MQ=255)
                                                                                                         tCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                          <  2:890278/148‑1 (MQ=255)
                                                                                                         tCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAAc                                          >  2:2370295/1‑148 (MQ=255)
                                                                                                          cAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACg                                         >  2:186078/1‑148 (MQ=255)
                                                                                                          cAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACg                                         >  2:3404416/1‑148 (MQ=255)
                                                                                                            gctttttCCTGAGCGCTTTCAACAGCTTCTCGCTGAGTGGGAAAATCGTCTATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGt                                       <  1:1379610/145‑1 (MQ=255)
                                                                                                            gctttgtCCTGTGCGCTTTCAACAGCTTCTCGCTGTGAGGGAAAATGGTCTATAACACCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGt                                       <  1:3124169/142‑1 (MQ=255)
                                                                                                                tttCCTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGc                                   >  2:793877/1‑148 (MQ=255)
                                                                                                                   ccTGAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCCACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTg                                <  2:383481/148‑1 (MQ=255)
                                                                                                                      gAGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGttt                             >  2:2722923/1‑148 (MQ=255)
                                                                                                                       aGCGCTTTCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTa                            >  2:2745021/1‑148 (MQ=255)
                                                                                                                              tccaCAGCTTCTCGCGGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc                     <  2:116059/145‑1 (MQ=255)
                                                                                                                              tCAACAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGc                     >  2:2905509/1‑148 (MQ=255)
                                                                                                                                aaCAGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCa                   <  1:2614054/148‑1 (MQ=255)
                                                                                                                                   aGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATgg                >  2:1140538/1‑148 (MQ=255)
                                                                                                                                   aGCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATgg                >  2:1140552/1‑148 (MQ=255)
                                                                                                                                    gCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc               >  1:2627593/1‑148 (MQ=255)
                                                                                                                                    gCTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGc               >  1:3294663/1‑148 (MQ=255)
                                                                                                                                     cTTCTCGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCg              >  1:1828175/1‑148 (MQ=255)
                                                                                                                                       tctcGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaa            >  1:922627/1‑148 (MQ=255)
                                                                                                                                       gcgcGCTGAGCGGGAAAATCGTCGATAACCTCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCACCGGTTGGCCTGTTTACAGGTGCCATGGCGaa            <  2:3019866/145‑1 (MQ=255)
                                                                                                                                        ctcGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGaaa           >  2:1910029/1‑148 (MQ=255)
                                                                                                                                          cGCTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaaga         <  2:161977/148‑1 (MQ=255)
                                                                                                                                            cTGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAaagaag       >  1:161079/1‑148 (MQ=255)
                                                                                                                                             tGAGAGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGt      <  2:3247216/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGCAGCGTCTGAAAGGCTTCGTTCTGCGTGCTGGTAAAGTGATCATCATCGTCAGCATTTTCCTGAGCGCTTTCAACAGCTTCTCGCTGAGCGGGAAAATCGTCGATAACATCAACGACTCGGCGCTGGCGTCCGTCAGCCGGGTGATCACCCCGGTCTTCAAGCCAATTGGCGTGCATGAAGATAACTGGCAGGCAACGGTTGGCCTGTTTACAGGTGCCATGGCGAAAGAAGTGGTA  >  CP009273/3535225‑3535517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑