Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,549,090 C→A 100% R883S (CGC→AGC)  malT → mal regulon transcriptional activator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,549,0900CA96.8% 664.6 / 11.7 220R883S (CGC→AGC) malTmal regulon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (3/4);  new base A (101/112);  total (104/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.54e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 220 total aligned reads displayed.

CCCGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAA  >  CP009273/3548945‑3549234
                                                                                                                                                 |                                                                                                                                                
cccGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGc                                                                                                                                                >  2:2539619/1‑148 (MQ=255)
    cTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAgg                                                                                                                                            <  1:3143025/148‑1 (MQ=255)
     tGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGa                                                                                                                                           <  2:1035001/148‑1 (MQ=255)
     tGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGa                                                                                                                                           >  1:2197634/1‑148 (MQ=255)
          cAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCgg                                                                                                                                      >  2:2009444/1‑148 (MQ=255)
          cAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCgg                                                                                                                                      <  2:2202239/148‑1 (MQ=255)
          cAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCgg                                                                                                                                      >  1:1736345/1‑148 (MQ=255)
           aaCGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGt                                                                                                                                     >  1:3293045/1‑148 (MQ=255)
           aaCGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGt                                                                                                                                     >  1:3276029/1‑148 (MQ=255)
            aCGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTa                                                                                                                                    >  1:2539928/1‑148 (MQ=255)
                 aaTGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGACACTCGGCGTGGCCCATAGCCAGGATGCGGTacaacc                                                                                                                               >  2:682626/1‑147 (MQ=255)
                  aTGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAacac                                                                                                                              >  2:1465945/1‑148 (MQ=255)
                   tGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAACCGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACg                                                                                                                             <  2:2102708/148‑1 (MQ=255)
                    ggCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGc                                                                                                                            >  1:3114608/1‑148 (MQ=255)
                    ggCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGc                                                                                                                            >  1:2403290/1‑148 (MQ=255)
                     gCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGcc                                                                                                                           <  1:1469952/148‑1 (MQ=255)
                      cAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGccc                                                                                                                          >  1:672740/1‑148 (MQ=255)
                           aCTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCaa                                                                                                                     >  2:3010868/1‑148 (MQ=255)
                           aCTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCaa                                                                                                                     <  2:583301/148‑1 (MQ=255)
                           aCTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCaa                                                                                                                     >  2:3011341/1‑148 (MQ=255)
                              ggggCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTg                                                                                                                  <  2:507245/148‑1 (MQ=255)
                                  cTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGa                                                                                                              <  1:1662017/148‑1 (MQ=255)
                                  cTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGa                                                                                                              >  2:2852067/1‑148 (MQ=255)
                                    gATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAg                                                                                                            <  2:2772897/148‑1 (MQ=255)
                                    gATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAg                                                                                                            <  2:2352136/148‑1 (MQ=255)
                                    gATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAg                                                                                                            <  1:1120028/148‑1 (MQ=255)
                                          ctctGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGatgatg                                                                                                      >  1:769013/1‑148 (MQ=255)
                                           tctGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAANCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATgg                                                                                                     <  1:1783653/148‑1 (MQ=255)
                                                ttACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTAc                                                                                                >  1:3475759/1‑148 (MQ=255)
                                                   cAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGc                                                                                             >  1:2131442/1‑148 (MQ=255)
                                                    aGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCg                                                                                            <  1:426637/148‑1 (MQ=255)
                                                     gCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCgt                                                                                           <  2:2455346/148‑1 (MQ=255)
                                                     gCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCgt                                                                                           >  1:1095164/1‑148 (MQ=255)
                                                      cAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAAGACGCCCAGCAATTGCTGAAGATGATGGGGt                                                                                                  <  1:2792166/140‑1 (MQ=255)
                                                      cAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCgtg                                                                                          >  2:260837/1‑148 (MQ=255)
                                                        aTGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAANCTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTa                                                                                        >  1:1785796/1‑148 (MQ=255)
                                                          gAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAg                                                                                      >  1:2433322/1‑148 (MQ=255)
                                                          gAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAg                                                                                      >  1:633034/1‑148 (MQ=255)
                                                           aGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGt                                                                                     >  2:1162736/1‑148 (MQ=255)
                                                           aGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGt                                                                                     <  1:885184/148‑1 (MQ=255)
                                                           aGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGt                                                                                     <  1:884814/148‑1 (MQ=255)
                                                            gCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGtt                                                                                    <  1:2441334/148‑1 (MQ=255)
                                                            gCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGtt                                                                                    <  1:2532724/148‑1 (MQ=255)
                                                            gCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGtt                                                                                    <  1:1763491/148‑1 (MQ=255)
                                                             gaaaTTGCCGGAGATCTGGAAGTCGCGGCAACCTCCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGttt                                                                                   <  1:511639/147‑1 (MQ=255)
                                                             cAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGttt                                                                                   <  2:30918/148‑1 (MQ=255)
                                                                aTTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAACCGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGc                                                                                <  1:3231199/148‑1 (MQ=255)
                                                                aTTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGc                                                                                >  2:3397538/1‑148 (MQ=255)
                                                                    ccGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTATCCGGa                                                                            >  2:3514973/1‑148 (MQ=255)
                                                                         gaACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAAcg                                                                       <  2:158872/148‑1 (MQ=255)
                                                                         gaACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAAcg                                                                       <  1:44778/148‑1 (MQ=255)
                                                                         gaACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAAcg                                                                       <  2:3060564/148‑1 (MQ=255)
                                                                          aaCTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAAcgc                                                                      >  2:2725176/1‑148 (MQ=255)
                                                                            cTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACgcgc                                                                    <  1:2333660/148‑1 (MQ=255)
                                                                            cTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACgcgc                                                                    <  1:2334050/148‑1 (MQ=255)
                                                                            cTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACgcgc                                                                    >  2:2173721/1‑148 (MQ=255)
                                                                               gAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAg                                                                 <  1:656497/148‑1 (MQ=255)
                                                                                 aGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGAt                                                               >  2:514356/1‑148 (MQ=255)
                                                                                  gTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATc                                                              >  2:139051/1‑148 (MQ=255)
                                                                                   tCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATcc                                                             >  1:3126002/1‑148 (MQ=255)
                                                                                      cgGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGc                                                          >  2:177854/1‑148 (MQ=255)
                                                                                      cgGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGc                                                          <  1:1357677/148‑1 (MQ=255)
                                                                                      cgGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGc                                                          <  2:3489619/148‑1 (MQ=255)
                                                                                       ggCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCt                                                         <  1:3137721/148‑1 (MQ=255)
                                                                                          aaCCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTAc                                                      <  1:2830805/148‑1 (MQ=255)
                                                                                           accaccATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACa                                                     >  2:1745684/1‑148 (MQ=255)
                                                                                             caccaTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACAtc                                                   >  2:1657613/1‑148 (MQ=255)
                                                                                              accaTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACAtct                                                  <  1:3234103/148‑1 (MQ=255)
                                                                                              accaTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACAtct                                                  <  2:3380018/148‑1 (MQ=255)
                                                                                               ccaTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACAtctc                                                 <  2:624153/148‑1 (MQ=255)
                                                                                                 aTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCCGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTg                                               >  2:669222/1‑148 (MQ=255)
                                                                                                 aTCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTg                                               >  2:669568/1‑148 (MQ=255)
                                                                                                  tCAACACGCATATCCGCAATCTGTATCATAAAATCGGCGTGGCCAATAGCAAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGTGGTTCTGAGTGTAATTTTATCGGGATAAAGCGCCAGATCCGGCTTACTTCTCTGc                                              <  2:3159976/148‑1 (MQ=255)
                                                                                                  tCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGc                                              <  1:1820871/148‑1 (MQ=255)
                                                                                                       aCGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGcatcat                                         <  2:2633019/148‑1 (MQ=255)
                                                                                                        cGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCAtt                                        <  2:1897041/148‑1 (MQ=255)
                                                                                                        cGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCAtt                                        <  1:68506/148‑1 (MQ=255)
                                                                                                          cATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCa                                      <  2:3293045/148‑1 (MQ=255)
                                                                                                          cATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCa                                      <  2:3276029/148‑1 (MQ=255)
                                                                                                          cATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCa                                      <  2:3209330/148‑1 (MQ=255)
                                                                                                           atatCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCaa                                     <  2:510833/148‑1 (MQ=255)
                                                                                                           atatCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCaa                                     >  1:3328793/1‑148 (MQ=255)
                                                                                                            tatCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTATGCATCATTCAAt                                    >  2:2082416/1‑148 (MQ=255)
                                                                                                             atCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATg                                   >  1:2474060/1‑148 (MQ=255)
                                                                                                                 gCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCa                               >  1:1044400/1‑148 (MQ=255)
                                                                                                                 gCAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCa                               >  2:2260180/1‑148 (MQ=255)
                                                                                                                  cAATCTGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCAc                              >  1:264815/1‑148 (MQ=255)
                                                                                                                       tGTATCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCcgcg                         <  1:260837/148‑1 (MQ=255)
                                                                                                                          aTCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTa                      >  2:793100/1‑148 (MQ=255)
                                                                                                                          aTCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTa                      <  1:1240195/148‑1 (MQ=255)
                                                                                                                          aTCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTa                      >  1:3485584/1‑148 (MQ=255)
                                                                                                                           tCAGAAACTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTAc                     >  1:30433/1‑148 (MQ=255)
                                                                                                                               aaaCTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCa                 <  1:2155275/148‑1 (MQ=255)
                                                                                                                                aaCTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCAt                <  2:452579/148‑1 (MQ=255)
                                                                                                                                aaCTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCAt                <  2:769013/148‑1 (MQ=255)
                                                                                                                                 aCTCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATc               >  1:1939065/1‑148 (MQ=255)
                                                                                                                                   tCGGCGTGGCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTg             <  2:3126002/148‑1 (MQ=255)
                                                                                                                                          ggCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTAt      >  1:1524505/1‑148 (MQ=255)
                                                                                                                                          ggCCCATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTAt      <  2:2433322/148‑1 (MQ=255)
                                                                                                                                              cATAGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCaaa  <  1:1626457/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCCGCTGACGCAACGTGAATGGCAGGTACTGGGGCTGATCTACTCTGGTTACAGCAATGAGCAAATTGCCGGAGAACTGGAAGTCGCGGCAACCACCATCAAAACGCATATCCGCAATCTGTATCAGAAACTCGGCGTGGCCCATCGCCAGGATGCGGTACAACACGCCCAGCAATTGCTGAAGATGATGGGGTACGGCGTGTAAGTTTAGCCGGATAACGCGCCAGATCCGGCTTACATCTCTGCATCATTCAATGCTCACCCGCGTTACGCCATCTGTTTCTATCAAA  >  CP009273/3548945‑3549234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑