Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,561,574 C→A 100% W372L (TGG→TTG)  glgC ← glucose‑1‑phosphate adenylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,561,5740CA98.8% 593.2 / ‑1.8 186W372L (TGG→TTG) glgCglucose‑1‑phosphate adenylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/3);  new base A (97/86);  total (97/89)
Fisher's exact test for biased strand distribution p-value = 1.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 187 total aligned reads displayed.

TTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGG  >  CP009273/3561429‑3561721
                                                                                                                                                 |                                                                                                                                                   
ttCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAAt                                                                                                                                                   <  1:498969/148‑1 (MQ=255)
 tCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAAGAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATa                                                                                                                                                  <  2:2637048/148‑1 (MQ=255)
    cgcttaaCCAGCACGTTGCATTCTTCTTAACTATACAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACtt                                                                                                                                               <  1:1197794/142‑1 (MQ=255)
           ccAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTaa                                                                                                                                        >  1:2556093/1‑148 (MQ=255)
             aGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACa                                                                                                                                      <  2:1179499/148‑1 (MQ=255)
              gcagtttGCCTTCTTCTGAACGTTAGAAACGACGTGCATCTTCCTCTTCGTTTTCACCAATCACGATGACTTCCGGAAGAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACTAGCGACCTACCAATACTTCCGGTAACaa                                                                                                                                     <  2:1028650/142‑1 (MQ=255)
              gCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTACCaa                                                                                                                                     >  2:115754/1‑148 (MQ=255)
               cACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAAt                                                                                                                                    >  2:3436050/1‑148 (MQ=255)
                 cGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAc                                                                                                                                  >  2:1983699/1‑148 (MQ=255)
                   aTGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcgg                                                                                                                                >  1:1035101/1‑148 (MQ=255)
                   aTGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcgg                                                                                                                                >  2:3172279/1‑148 (MQ=255)
                   aTGCCTTCTTCTAAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcgg                                                                                                                                >  2:3321544/1‑148 (MQ=255)
                      ccTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATAcggcgg                                                                                                                             >  1:862065/1‑148 (MQ=255)
                            tctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCaa                                                                                                                       >  1:3073820/1‑148 (MQ=255)
                             ctGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAAt                                                                                                                      >  1:264097/1‑148 (MQ=255)
                              tGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATg                                                                                                                     <  2:897888/148‑1 (MQ=255)
                                  cGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGc                                                                                                                 >  1:1504869/1‑148 (MQ=255)
                                  cGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGc                                                                                                                 <  1:2056386/148‑1 (MQ=255)
                                      aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaa                                                                                                             >  2:2974090/1‑148 (MQ=255)
                                      aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaa                                                                                                             <  1:3367183/148‑1 (MQ=255)
                                      aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaa                                                                                                             >  1:3011684/1‑148 (MQ=255)
                                      aGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaa                                                                                                             >  1:2360698/1‑148 (MQ=255)
                                        aaaCGACGTGCATCTTCCTCTGCTTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatg                                                                                                           <  1:740647/148‑1 (MQ=255)
                                         aaCGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatga                                                                                                          <  1:1701487/148‑1 (MQ=255)
                                           cgacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAgaatgaat                                                                                                        >  1:3109769/1‑148 (MQ=255)
                                           cgacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGCTAACAATACGGCGGAATCAATGTTGCAgaatgaat                                                                                                        >  1:402922/1‑148 (MQ=255)
                                            gacgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAAtt                                                                                                       <  2:875009/148‑1 (MQ=255)
                                             acgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGGATGAATTc                                                                                                      >  1:789928/1‑148 (MQ=255)
                                              cgTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCa                                                                                                     <  1:2714664/148‑1 (MQ=255)
                                                tGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcg                                                                                                   >  1:3104748/1‑148 (MQ=255)
                                                 gCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcgc                                                                                                  >  1:1567105/1‑148 (MQ=255)
                                                 gCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcgc                                                                                                  >  1:1473793/1‑148 (MQ=255)
                                                 gCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcgc                                                                                                  >  1:3306881/1‑148 (MQ=255)
                                                  cATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCAcgcg                                                                                                 >  2:1655516/1‑148 (MQ=255)
                                                   aTCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGa                                                                                                <  2:862065/148‑1 (MQ=255)
                                                   aTCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGa                                                                                                <  1:1924658/148‑1 (MQ=255)
                                                    tCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGaa                                                                                               <  2:537203/148‑1 (MQ=255)
                                                        ccTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAAcgcg                                                                                           <  1:1625370/148‑1 (MQ=255)
                                                         ctctGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGACcgcgc                                                                                          >  1:2475312/1‑148 (MQ=255)
                                                             gCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAAAACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCgaga                                                                                      >  1:2063892/1‑148 (MQ=255)
                                                               gTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaac                                                                                    <  2:1966270/148‑1 (MQ=255)
                                                                ttttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaaca                                                                                   >  1:332270/1‑148 (MQ=255)
                                                                ttttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaaca                                                                                   >  1:2031116/1‑148 (MQ=255)
                                                                 tttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacag                                                                                  >  1:244854/1‑148 (MQ=255)
                                                                 tttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacag                                                                                  >  1:2756985/1‑148 (MQ=255)
                                                                 tttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacag                                                                                  >  2:3417021/1‑148 (MQ=255)
                                                                  ttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacaga                                                                                 <  2:704752/148‑1 (MQ=255)
                                                                  ttCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacaga                                                                                 <  1:1261203/148‑1 (MQ=255)
                                                                   tCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacagaa                                                                                <  1:2331556/148‑1 (MQ=255)
                                                                   tCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacagaa                                                                                >  2:3434583/1‑148 (MQ=255)
                                                                    cACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGagaacagaac                                                                               >  2:1122241/1‑148 (MQ=255)
                                                                      ccAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACgg                                                                             >  1:44664/1‑148 (MQ=255)
                                                                       cAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGa                                                                            <  2:383250/148‑1 (MQ=255)
                                                                         aTCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACt                                                                          >  2:22047/1‑148 (MQ=255)
                                                                          tCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAANACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTg                                                                         >  1:1780652/1‑148 (MQ=255)
                                                                           cACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGc                                                                        >  1:202663/1‑148 (MQ=255)
                                                                                 gCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccacc                                                                  >  2:670976/1‑148 (MQ=255)
                                                                                 gCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGcaccacc                                                                  >  2:3474257/1‑148 (MQ=255)
                                                                                    ttCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCaccaccacc                                                               >  1:1044864/1‑148 (MQ=255)
                                                                                       cGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGaa                                                            <  2:111333/148‑1 (MQ=255)
                                                                                       cGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGaa                                                            >  1:3001186/1‑148 (MQ=255)
                                                                                        ggAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAc                                                           >  2:2102214/1‑148 (MQ=255)
                                                                                         gAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAcc                                                          >  1:1948028/1‑148 (MQ=255)
                                                                                         gAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAAcc                                                          <  1:3321544/148‑1 (MQ=255)
                                                                                          aaTAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCg                                                         >  1:717585/1‑148 (MQ=255)
                                                                                           aTAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCgg                                                        >  1:1284825/1‑148 (MQ=255)
                                                                                            tAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGga                                                       >  1:686509/1‑148 (MQ=255)
                                                                                             aaCACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGgag                                                      >  2:1452151/1‑148 (MQ=255)
                                                                                              acacAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGgaga                                                     >  2:1617178/1‑148 (MQ=255)
                                                                                                acaAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGACCAGAACGGACTGCACCACCACCGAACCGGAGATc                                                   >  2:1904789/1‑148 (MQ=255)
                                                                                                      aCGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATcacacac                                             <  2:144803/148‑2 (MQ=255)
                                                                                                      aCGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACaa                                             <  2:1013330/148‑1 (MQ=255)
                                                                                                       cgatcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAc                                            >  2:2035978/1‑148 (MQ=255)
                                                                                                       cgatcgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAc                                            <  1:1632706/148‑1 (MQ=255)
                                                                                                           cgatGACGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAAccgcc                                        >  2:2845385/1‑148 (MQ=255)
                                                                                                                aCGCAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGaaa                                   <  2:2714258/148‑1 (MQ=255)
                                                                                                                   cAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCa                                <  1:115333/148‑1 (MQ=255)
                                                                                                                   cAGCGGCGCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCa                                <  2:2992238/148‑1 (MQ=255)
                                                                                                                     gcggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGt                              <  1:873850/148‑1 (MQ=255)
                                                                                                                      cggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTg                             <  1:1068691/148‑1 (MQ=255)
                                                                                                                      cggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTg                             <  2:598448/148‑1 (MQ=255)
                                                                                                                      cggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTg                             >  2:157061/1‑148 (MQ=255)
                                                                                                                      cggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTg                             <  2:1087574/148‑1 (MQ=255)
                                                                                                                      cggcgCAGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTg                             <  1:991877/148‑1 (MQ=255)
                                                                                                                       ggcgCAGACGGCACGAGCGACCTACCAAGACGTCCGGTAACAATACGGCGGAATCACTGTTGCAGAATGAAGTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGa                            <  2:3419262/148‑1 (MQ=255)
                                                                                                                           cAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTa                        <  2:1755882/148‑1 (MQ=255)
                                                                                                                            aGACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTaa                       >  2:1026418/1‑148 (MQ=255)
                                                                                                                             gACGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAg                      <  2:1473793/148‑1 (MQ=255)
                                                                                                                              aCGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAgg                     <  2:202663/148‑1 (MQ=255)
                                                                                                                              aCGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAgg                     <  2:3090506/148‑1 (MQ=255)
                                                                                                                              aCGGCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAgg                     <  2:2466557/148‑1 (MQ=255)
                                                                                                                                 gCACGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTc                  >  2:582067/1‑148 (MQ=255)
                                                                                                                                    cGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATc               >  2:1669708/1‑148 (MQ=255)
                                                                                                                                    cGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATc               >  1:380160/1‑148 (MQ=255)
                                                                                                                                    cGAGCGACCTACCAATACTTCCGGTAACAATACGGCGGAATAAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATc               <  1:12773/148‑1 (MQ=255)
                                                                                                                                       gCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCg            <  1:3114384/148‑1 (MQ=255)
                                                                                                                                       gCGACCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCg            <  1:3114455/148‑1 (MQ=255)
                                                                                                                                          aCCTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTgg         >  2:401256/1‑148 (MQ=255)
                                                                                                                                            cTACCAATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCt       >  1:1802943/1‑148 (MQ=255)
                                                                                                                                                 cATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGACCGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCgg  <  2:846016/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TTCGCGCGTTACCAGCACGATGCCTTCTTCTGAACGATAGAAACGACGTGCATCTTCCTCTGCGTTTTCACCAATCACCATGCCTTCCGGAATAACACAAGCACGATCGATGACGCAGCGGCGCAGACGGCACGAGCGACCTACCCATACTTCCGGTAACAATACGGCGGAATCAATGTTGCAGAATGAATTCACGCGAACGCGCGAGAACAGAACGGACTGCACCACCACCGAACCGGAGATCACACAACCGCCGGAAACCAGTGAGTTAAGGGTCATCCCGTGGCTACCGG  >  CP009273/3561429‑3561721

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑