Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 262,114 C→A 100% D51Y (GAT→TAT)  yafY ← lipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273262,1140CA97.9% 640.4 / 3.2 205D51Y (GAT→TAT) yafYlipoprotein, inner membrane; overproduction stimulates degP expression; CP4‑6 prophage
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base A (81/119);  total (85/120)
Fisher's exact test for biased strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

TCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTA  >  CP009273/261970‑262256
                                                                                                                                                |                                                                                                                                              
tcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACataatc                                                                                                                                             <  1:2437191/148‑1 (MQ=255)
tcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACataatc                                                                                                                                             >  2:1166874/1‑148 (MQ=255)
tcaGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACataatc                                                                                                                                             <  2:1067474/148‑1 (MQ=255)
 caGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATcc                                                                                                                                            <  1:378676/148‑1 (MQ=255)
  aGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCt                                                                                                                                           >  1:2204380/1‑148 (MQ=255)
  aGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCt                                                                                                                                           <  1:201846/148‑1 (MQ=255)
   gTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtg                                                                                                                                          <  1:136025/148‑1 (MQ=255)
   gTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtg                                                                                                                                          <  1:148494/148‑1 (MQ=255)
   gTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtg                                                                                                                                          >  1:1900719/1‑148 (MQ=255)
      gCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtggtg                                                                                                                                       >  2:2645644/1‑148 (MQ=255)
      gCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCtggtg                                                                                                                                       >  2:2434441/1‑148 (MQ=255)
        tGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGc                                                                                                                                     <  1:3207332/148‑1 (MQ=255)
        tGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGc                                                                                                                                     <  2:2881607/148‑1 (MQ=255)
          gACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTg                                                                                                                                   >  2:1420653/1‑148 (MQ=255)
          gACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTg                                                                                                                                   >  2:3240347/1‑148 (MQ=255)
           aCGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGa                                                                                                                                  >  2:2859718/1‑148 (MQ=255)
             ggTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGAcc                                                                                                                                >  1:3160248/1‑148 (MQ=255)
              gTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACca                                                                                                                               >  2:1495634/1‑148 (MQ=255)
                  tttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATc                                                                                                                           <  1:2872412/148‑1 (MQ=255)
                   ttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCa                                                                                                                          <  1:2041410/148‑1 (MQ=255)
                   ttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCa                                                                                                                          <  1:269420/148‑1 (MQ=255)
                   ttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCa                                                                                                                          <  1:1156958/148‑1 (MQ=255)
                   ttCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCa                                                                                                                          >  1:1102415/1‑148 (MQ=255)
                      cTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTg                                                                                                                       <  2:3121815/148‑1 (MQ=255)
                       tGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGa                                                                                                                      >  2:3177971/1‑148 (MQ=255)
                        gCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGaa                                                                                                                     <  2:2090075/148‑1 (MQ=255)
                         cATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGaaa                                                                                                                    <  2:1818347/148‑1 (MQ=255)
                            atCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAAcgc                                                                                                                 >  1:2844341/1‑148 (MQ=255)
                            atCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAAcgc                                                                                                                 >  2:777031/1‑148 (MQ=255)
                              ccACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCgg                                                                                                               <  2:2279253/148‑1 (MQ=255)
                                aCGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTg                                                                                                             <  1:3240735/148‑1 (MQ=255)
                                    ggTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGt                                                                                                         >  2:3096091/1‑148 (MQ=255)
                                      tagtagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGa                                                                                                       >  2:872748/1‑148 (MQ=255)
                                         tagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGt                                                                                                    <  1:2303462/148‑1 (MQ=255)
                                         tagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGt                                                                                                    >  1:1350401/1‑148 (MQ=255)
                                         tagtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGt                                                                                                    >  1:3320976/1‑148 (MQ=255)
                                          agtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTc                                                                                                   <  1:31951/148‑1 (MQ=255)
                                          agtaACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCAGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTc                                                                                                   <  2:1698714/148‑1 (MQ=255)
                                               cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTaa                                                                                              >  2:1565020/1‑148 (MQ=255)
                                               cTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTaa                                                                                              >  1:2181445/1‑148 (MQ=255)
                                                      ccTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACt                                                                                       >  2:1654929/1‑148 (MQ=255)
                                                      ccTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACt                                                                                       <  1:1662046/148‑1 (MQ=255)
                                                       cTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTg                                                                                      <  1:1895283/148‑1 (MQ=255)
                                                        tCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGa                                                                                     >  2:2703293/1‑148 (MQ=255)
                                                            aGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGc                                                                                 <  1:382616/148‑1 (MQ=255)
                                                            aGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGc                                                                                 <  1:2625603/148‑1 (MQ=255)
                                                             gCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGcc                                                                                >  2:2186355/1‑148 (MQ=255)
                                                             gCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGcc                                                                                >  1:1581801/1‑148 (MQ=255)
                                                               cATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCtt                                                                              >  2:484951/1‑148 (MQ=255)
                                                               cATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCtt                                                                              >  2:933797/1‑148 (MQ=255)
                                                                aTCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTc                                                                             <  2:2685245/148‑1 (MQ=255)
                                                                 tCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCa                                                                            >  1:3015802/1‑148 (MQ=255)
                                                                  cGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAg                                                                           >  1:1032980/1‑148 (MQ=255)
                                                                    ggCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGt                                                                         >  2:564331/1‑148 (MQ=255)
                                                                    ggCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGt                                                                         <  2:1289108/148‑1 (MQ=255)
                                                                       gACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtcat                                                                      <  2:588644/148‑1 (MQ=255)
                                                                         cTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGtcatca                                                                    >  2:1522319/1‑148 (MQ=255)
                                                                            gCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACtt                                                                 <  1:154040/148‑1 (MQ=255)
                                                                            gCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACtt                                                                 <  1:3057989/148‑1 (MQ=255)
                                                                                       aaaCCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCa                                                      <  1:836797/148‑1 (MQ=255)
                                                                                       aaaCCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCa                                                      <  1:174687/148‑1 (MQ=255)
                                                                                         aCCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATg                                                    <  2:3320976/148‑1 (MQ=255)
                                                                                           cTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCTCATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCg                                                  <  2:1553348/148‑1 (MQ=255)
                                                                                           cTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCg                                                  <  1:745445/148‑1 (MQ=255)
                                                                                                aGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                             <  2:346711/148‑1 (MQ=255)
                                                                                                aGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                             >  1:1734593/1‑148 (MQ=255)
                                                                                                aGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                             <  2:345172/148‑1 (MQ=255)
                                                                                                aGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                             <  2:2218072/148‑1 (MQ=255)
                                                                                                aGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgag                                             <  2:278514/148‑1 (MQ=255)
                                                                                                 ggTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATgaga                                            >  1:769509/1‑148 (MQ=255)
                                                                                                    gggACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAAc                                         <  2:1302047/148‑1 (MQ=255)
                                                                                                    gggACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAAc                                         <  1:2917080/148‑1 (MQ=255)
                                                                                                    gggACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAAc                                         <  1:1026173/148‑1 (MQ=255)
                                                                                                     ggACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACa                                        >  1:1995867/1‑148 (MQ=255)
                                                                                                          ggATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtg                                   <  2:1900719/148‑1 (MQ=255)
                                                                                                          ggATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtg                                   <  1:2709976/148‑1 (MQ=255)
                                                                                                           gATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtgg                                  <  2:843959/148‑1 (MQ=255)
                                                                                                            aTAAGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtggt                                 >  2:3351883/1‑148 (MQ=255)
                                                                                                              aaGTAGTCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGtggtgg                               >  1:688828/1‑148 (MQ=255)
                                                                                                               agtagtCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGc                              >  1:2424928/1‑148 (MQ=255)
                                                                                                                 tagtCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAATTTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCAGGCGATGAGAAACAGAGTGGTGGcga                            >  2:1816393/1‑146 (MQ=255)
                                                                                                                  agtCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAgtggt                                 >  1:3097298/1‑142 (MQ=255)
                                                                                                                   gtCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAAcc                          <  2:40720/148‑1 (MQ=255)
                                                                                                                    tCAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCa                         >  1:2911725/1‑148 (MQ=255)
                                                                                                                     cAACCTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAg                        >  2:61196/1‑148 (MQ=255)
                                                                                                                        ccTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAgcgc                     <  1:638005/148‑1 (MQ=255)
                                                                                                                        ccTGTGACCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAgcgc                     <  2:719782/148‑1 (MQ=255)
                                                                                                                              aCCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCcagcag               <  2:3015802/148‑1 (MQ=255)
                                                                                                                              aCCATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCcagcag               <  1:3267769/148‑1 (MQ=255)
                                                                                                                               ccATTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCaga              >  1:1846660/1‑148 (MQ=255)
                                                                                                                                 aTTCTTCGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCACCAGAgg            >  1:43091/1‑148 (MQ=255)
                                                                                                                                    cttcGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAg         >  2:3094672/1‑148 (MQ=255)
                                                                                                                                     ttcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgt        <  1:2050316/148‑1 (MQ=255)
                                                                                                                                     ttcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgt        <  1:3081974/148‑1 (MQ=255)
                                                                                                                                     ttcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgt        <  2:967409/148‑1 (MQ=255)
                                                                                                                                      tcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgtg       <  2:182647/148‑1 (MQ=255)
                                                                                                                                      tcGCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAgtg       <  2:111402/148‑1 (MQ=255)
                                                                                                                                        gCTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGtt     <  1:1420653/148‑1 (MQ=255)
                                                                                                                                         cTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGttt    <  1:3501401/148‑1 (MQ=255)
                                                                                                                                         cTGACATAATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGttt    <  2:213460/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                              
TCAGTGGCTGGACGGTTTTTTCCTGCATATCCACGAGGTAGTAGTAACTGCCATCCTCGCAGCCATCGGGCGACTGGCGGGTACGTAAAACCTGCAAGGTGGGACCGGATAAGTAGTCAACCTGTGACCATTCTTCGCTGACATCATCCTGGTGGCTGACCACATCACTGAAACGCGGTGGGGTGAGGTCCTTAAATTTACTGATGGCCTTCAGGTCATCACTTCTGTCATCGCATGCGATGAGAAACAGAGTGGTGGCAACCAGCGCCAGCAGAGGCAGTGTTTTA  >  CP009273/261970‑262256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑