Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,582,807 G→T 100% P194Q (CCG→CAG)  ugpE ← glycerol‑3‑phosphate transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,582,8070GT99.1% 735.1 / ‑2.1 224P194Q (CCG→CAG) ugpEglycerol‑3‑phosphate transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (120/102);  total (122/102)
Fisher's exact test for biased strand distribution p-value = 5.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

TCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGG  >  CP009273/3582663‑3582951
                                                                                                                                                |                                                                                                                                                
   ccTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCAACAACGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGaaaaa                                                                                                                                            <  2:223929/148‑1 (MQ=255)
    cTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAAc                                                                                                                                           <  2:2571616/148‑1 (MQ=255)
      gTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGa                                                                                                                                         <  1:1870975/148‑1 (MQ=255)
       tAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGAt                                                                                                                                        >  1:2823643/1‑148 (MQ=255)
        aGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATg                                                                                                                                       <  1:2340666/148‑1 (MQ=255)
              catcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCaa                                                                                                                                 >  2:1976980/1‑148 (MQ=255)
              catcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCaa                                                                                                                                 >  1:2746144/1‑148 (MQ=255)
                tcCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCaaaa                                                                                                                               >  1:974590/1‑148 (MQ=255)
                 cccTTGGATCCCTGCCACGGTGGTGCCGAGAACCACATCGGAAATAACCAACCACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACCGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaag                                                                                                                              <  1:1250195/148‑1 (MQ=255)
                   cTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAaagaa                                                                                                                            >  2:623441/1‑148 (MQ=255)
                        aTCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATACAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCa                                                                                                                       >  1:2008131/1‑148 (MQ=255)
                        aTCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCa                                                                                                                       >  1:3060352/1‑148 (MQ=255)
                         tCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAt                                                                                                                      >  2:1223612/1‑148 (MQ=255)
                         tCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAt                                                                                                                      <  1:1316832/148‑1 (MQ=255)
                         tCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCAt                                                                                                                      <  2:2457058/148‑1 (MQ=255)
                         tCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCAt                                                                                                                      >  2:417066/1‑148 (MQ=255)
                            cTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTgg                                                                                                                   <  2:3469570/148‑1 (MQ=255)
                              gCCACGGTGGTGCCTAGATCCACATCGTTAATAATCACCCACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCg                                                                                                                 <  1:606081/148‑1 (MQ=255)
                              gCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCg                                                                                                                 >  2:2094482/1‑148 (MQ=255)
                               ccACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGa                                                                                                                <  2:1935372/148‑1 (MQ=255)
                                 aCGGTGGTGCCGAGATCCACATCGGTAATAATCACCAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATg                                                                                                              <  1:907462/148‑1 (MQ=255)
                                 aCGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATg                                                                                                              <  2:2367506/148‑1 (MQ=255)
                                 aCGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATg                                                                                                              <  1:2040525/148‑1 (MQ=255)
                                     tggtgCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGcc                                                                                                          >  2:507876/1‑148 (MQ=255)
                                          ccGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGa                                                                                                     >  2:3395742/1‑148 (MQ=255)
                                          ccGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGa                                                                                                     <  2:2877092/148‑1 (MQ=255)
                                           cGAGATCCACATCGGTAATAATCAACCACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAACCAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGAt                                                                                                    <  1:2281077/148‑1 (MQ=255)
                                                tCCACATCGGTAATAATCAACACCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcg                                                                                               <  1:1439550/148‑1 (MQ=255)
                                                tCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcg                                                                                               <  1:1651466/148‑1 (MQ=255)
                                                tCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcg                                                                                               <  1:2238560/148‑1 (MQ=255)
                                                tCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcg                                                                                               <  1:2238776/148‑1 (MQ=255)
                                                tCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcg                                                                                               <  1:568197/148‑1 (MQ=255)
                                                tCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcg                                                                                               <  1:566004/148‑1 (MQ=255)
                                                tCCACATCGGTAATAATCAACAACGGCCACAAATACTGAATCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCcgcg                                                                                               >  1:2379125/1‑148 (MQ=255)
                                                   acaTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCgg                                                                                            >  1:1359061/1‑148 (MQ=255)
                                                    caTCGGTAATAATCAACACCGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCNGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGc                                                                                           <  1:1782232/148‑1 (MQ=255)
                                                     aTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCt                                                                                          >  2:1142329/1‑148 (MQ=255)
                                                     aTCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCt                                                                                          <  1:3097700/148‑1 (MQ=255)
                                                          taataaTCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTccac                                                                                     >  1:628704/1‑148 (MQ=255)
                                                             taatCAACAACGGCCACACATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAg                                                                                  <  2:2111549/148‑1 (MQ=255)
                                                             taatCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAg                                                                                  <  2:1241463/148‑1 (MQ=255)
                                                                tCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTc                                                                               >  1:3213421/1‑148 (MQ=255)
                                                                   acaacGGCCACAAATACTGATTCCAGCCGTAGATAAAGTTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCTATCAGCGCGGCTTCCACCAGCTCATc                                                                            >  1:716337/1‑148 (MQ=255)
                                                                       cGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGc                                                                        >  1:3028435/1‑148 (MQ=255)
                                                                       cGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGc                                                                        <  1:2532394/148‑1 (MQ=255)
                                                                         gCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAg                                                                      >  2:898246/1‑148 (MQ=255)
                                                                         gCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAg                                                                      >  1:1710279/1‑148 (MQ=255)
                                                                          ccACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGc                                                                     >  2:277334/1‑148 (MQ=255)
                                                                            acaAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGt                                                                   <  1:2845253/148‑1 (MQ=255)
                                                                            acaAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGt                                                                   <  1:1094771/148‑1 (MQ=255)
                                                                              aaaTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCa                                                                 <  1:2490575/148‑1 (MQ=255)
                                                                               aaTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCAt                                                                >  1:248673/1‑148 (MQ=255)
                                                                                aTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATa                                                               <  2:1645519/148‑1 (MQ=255)
                                                                                aTACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATa                                                               >  2:1580447/1‑148 (MQ=255)
                                                                                  aCTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaaa                                                             >  1:264854/1‑148 (MQ=255)
                                                                                  aCTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATaaa                                                             >  1:646619/1‑148 (MQ=255)
                                                                                   cTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAg                                                            <  1:58956/148‑1 (MQ=255)
                                                                                    tGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGa                                                           >  2:2695316/1‑148 (MQ=255)
                                                                                     gATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGaa                                                          >  1:751178/1‑148 (MQ=255)
                                                                                     gATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGaa                                                          <  2:2955785/148‑1 (MQ=255)
                                                                                      aTTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAAc                                                         >  1:2922557/1‑148 (MQ=255)
                                                                                      aTTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAAc                                                         >  1:647401/1‑148 (MQ=255)
                                                                                      aTTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAAc                                                         <  2:1567270/148‑1 (MQ=255)
                                                                                       ttCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACt                                                        <  1:834970/148‑1 (MQ=255)
                                                                                       ttCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACt                                                        <  1:432992/148‑1 (MQ=255)
                                                                                        tCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTg                                                       >  2:588717/1‑148 (MQ=255)
                                                                                              cGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGaa                                                 >  1:1179271/1‑148 (MQ=255)
                                                                                                tAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACa                                               >  2:2580447/1‑148 (MQ=255)
                                                                                                 aGATAAAGGTGATCACAAACAGTGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAg                                              >  2:3254012/1‑148 (MQ=255)
                                                                                                   aTAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGa                                            >  2:1484452/1‑148 (MQ=255)
                                                                                                   aTAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGa                                            >  2:1482970/1‑148 (MQ=255)
                                                                                                     aaaGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGaaa                                          <  1:323039/148‑1 (MQ=255)
                                                                                                      aaGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAg                                         >  1:1977380/1‑148 (MQ=255)
                                                                                                       aGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAACAACTGCCGGACCAGGcaata                                        >  2:2506441/1‑146 (MQ=255)
                                                                                                        ggTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGACCAGGAAAGTa                                       >  2:2071310/1‑148 (MQ=255)
                                                                                                        ggTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTa                                       >  1:45635/1‑148 (MQ=255)
                                                                                                        ggTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTa                                       >  1:1017644/1‑148 (MQ=255)
                                                                                                          tGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGc                                     >  2:2641778/1‑148 (MQ=255)
                                                                                                          tGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGc                                     <  1:507585/148‑1 (MQ=255)
                                                                                                           gATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCg                                    >  2:3416231/1‑148 (MQ=255)
                                                                                                            aTCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCgg                                   <  1:1005286/148‑1 (MQ=255)
                                                                                                             tCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGt                                  <  2:3032694/148‑1 (MQ=255)
                                                                                                                caAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGc                               >  1:2212126/1‑148 (MQ=255)
                                                                                                                caAACAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGc                               >  1:2929156/1‑148 (MQ=255)
                                                                                                                  aaCAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCg                             <  2:2823643/148‑1 (MQ=255)
                                                                                                                   aCAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGa                            >  2:44518/1‑148 (MQ=255)
                                                                                                                   aCAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGa                            >  1:1152128/1‑148 (MQ=255)
                                                                                                                   aCAGCGCCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGa                            >  2:411331/1‑148 (MQ=255)
                                                                                                                      gcgcCGCCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGc                         >  1:2630080/1‑148 (MQ=255)
                                                                                                                       cgccgcCAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGcc                        >  2:2032185/1‑148 (MQ=255)
                                                                                                                          cgccAGATTAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATc                     <  2:716338/148‑1 (MQ=255)
                                                                                                                                 ttAGTTTTTGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCa              >  2:2435892/1‑148 (MQ=255)
                                                                                                                                  tAGTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAg             >  1:3033353/1‑148 (MQ=255)
                                                                                                                                    gTTTTGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCg           <  2:3100514/148‑1 (MQ=255)
                                                                                                                                     ttttGGAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGt          <  1:2674015/148‑1 (MQ=255)
                                                                                                                                         ggAGAGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTaaa      <  1:3490590/148‑1 (MQ=255)
                                                                                                                                          gagaGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAAc     <  1:3262355/148‑1 (MQ=255)
                                                                                                                                          gagaGCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAAc     >  1:526633/1‑148 (MQ=255)
                                                                                                                                             agCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCgg  >  1:134296/1‑148 (MQ=255)
                                                                                                                                             agCTGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCgg  >  2:1802079/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
TCGCCTGTAGCGATCATCCCTTTGATCCCTGCCACGGTGGTGCCGAGATCCACATCGGTAATAATCAACAACGGCCACAAATACTGATTCCAGCCGTAGATAAAGGTGATCACAAACAGCGCCGCCAGATTAGTTTTGGAGAGCGGAAAAACGATGTCGCAAAAGAAGCGCATTGGCGATGCGCCGTCGATCCGCGCGGCTTCCACCAGCTCATCCGGCAGCGTCATAAAGAACTGGCGGAACAGGAAAGTAGCGGTCGCCGAGGCCATCAGCGGCAGCGTTAAACCGG  >  CP009273/3582663‑3582951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑