Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,590,636 G→T 100% Y95* (TAC→TAA)  livK ← leucine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,590,6360GT98.4% 762.7 / 1.4 239Y95* (TAC→TAA) livKleucine transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (2/2);  new base T (124/111);  total (126/113)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 239 total aligned reads displayed.

CCAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACGCGCGCCG  >  CP009273/3590491‑3590780
                                                                                                                                                 |                                                                                                                                                
ccAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTa                                                                                                                                                <  2:1264895/148‑1 (MQ=255)
 cAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTAt                                                                                                                                               >  2:2390517/1‑148 (MQ=255)
    cccGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTa                                                                                                                                            <  1:3455563/148‑1 (MQ=255)
    cccGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTa                                                                                                                                            <  2:1744292/148‑1 (MQ=255)
      cGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAAt                                                                                                                                          <  1:269478/148‑1 (MQ=255)
        gCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGc                                                                                                                                        <  1:2379322/148‑1 (MQ=255)
        gCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGc                                                                                                                                        <  1:219208/148‑1 (MQ=255)
         cAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGcc                                                                                                                                       <  1:963219/148‑1 (MQ=255)
             aCGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCa                                                                                                                                   >  2:2167055/1‑148 (MQ=255)
              cGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCAGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCTGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCAt                                                                                                                                  <  2:511427/148‑1 (MQ=255)
                 aTAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTaa                                                                                                                               <  1:3499747/148‑1 (MQ=255)
                   aaTGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACg                                                                                                                             >  2:3167937/1‑148 (MQ=255)
                   aaTGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACg                                                                                                                             >  1:2299084/1‑148 (MQ=255)
                   aaTGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACg                                                                                                                             <  1:1203461/148‑1 (MQ=255)
                      gtgtTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGAtt                                                                                                                          >  1:1764298/1‑148 (MQ=255)
                      gtgtTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGAtt                                                                                                                          >  1:921377/1‑148 (MQ=255)
                        gtTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGAtttt                                                                                                                        >  1:468715/1‑148 (MQ=255)
                         ttGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTttg                                                                                                                       >  2:2674185/1‑148 (MQ=255)
                         ttGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTttg                                                                                                                       >  2:2889843/1‑148 (MQ=255)
                         ttGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTttg                                                                                                                       >  2:3130295/1‑148 (MQ=255)
                         ttGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTttg                                                                                                                       <  1:2665007/148‑1 (MQ=255)
                         ttGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTttg                                                                                                                       >  1:3100509/1‑148 (MQ=255)
                          tGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTttgt                                                                                                                      <  2:1218493/148‑1 (MQ=255)
                                ccGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGTCATTAACGATTTTGTTGGCGa                                                                                                                >  1:660515/1‑148 (MQ=255)
                                      tGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCaa                                                                                                          >  2:888392/1‑148 (MQ=255)
                                         gTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACgg                                                                                                       >  2:742513/1‑148 (MQ=255)
                                          tCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGc                                                                                                      >  2:937217/1‑148 (MQ=255)
                                          tCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGc                                                                                                      <  2:3355952/148‑1 (MQ=255)
                                              cTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGt                                                                                                  >  1:445877/1‑148 (MQ=255)
                                              cTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGt                                                                                                  <  2:2925518/148‑1 (MQ=255)
                                              cTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGt                                                                                                  >  2:635618/1‑148 (MQ=255)
                                               tCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGtt                                                                                                 <  1:3039778/148‑1 (MQ=255)
                                                ccGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGttt                                                                                                <  1:3205781/148‑1 (MQ=255)
                                                ccGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGttt                                                                                                <  1:109935/148‑1 (MQ=255)
                                                ccGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGttt                                                                                                <  1:1661670/148‑1 (MQ=255)
                                                ccGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGttt                                                                                                <  1:1661753/148‑1 (MQ=255)
                                                 cGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTc                                                                                               >  2:3386228/1‑148 (MQ=255)
                                                 cGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTc                                                                                               >  1:806654/1‑148 (MQ=255)
                                                  gggTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCg                                                                                              <  1:2349306/148‑1 (MQ=255)
                                                   ggTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCgg                                                                                             >  2:3381424/1‑148 (MQ=255)
                                                             tCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGcg                                                                                   <  1:835048/148‑1 (MQ=255)
                                                             tCCCGGAGAGATCATCAGAATACCTTATTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGTACACAGATGACCAATAACTTATTTAATGCCTTCATTAACGATTTTGTTGGCGACCGCAACGGGTTGTTTCGGGTCGCATGcg                                                                                   <  1:1183247/148‑1 (MQ=255)
                                                              cccGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGcgt                                                                                  <  1:2889843/148‑1 (MQ=255)
                                                              cccGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGcgt                                                                                  <  1:1264578/148‑1 (MQ=255)
                                                                      gaTCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCatat                                                                          >  2:169808/1‑148 (MQ=255)
                                                                       atcatcAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATAtt                                                                         >  2:2492140/1‑148 (MQ=255)
                                                                        tcatcaGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTc                                                                        >  2:1932019/1‑148 (MQ=255)
                                                                            caGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACg                                                                    >  2:2347995/1‑148 (MQ=255)
                                                                               aaTACCTACGTCATCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGACATTAACGATTATGTTGGCGACCGCACCGGCTTGTATCGGGTCGCATGCGTCCTCATAGTACACGCCa                                                                 >  2:2798803/1‑148 (MQ=255)
                                                                                 tACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAAc                                                               <  2:1698314/148‑1 (MQ=255)
                                                                                 tACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAAc                                                               <  2:1455432/148‑1 (MQ=255)
                                                                                 tACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATGTAATGCCGTCATTAAAGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAAc                                                               >  1:3246071/1‑148 (MQ=255)
                                                                                  aCCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAAcc                                                              >  2:3008875/1‑148 (MQ=255)
                                                                                     ttCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGt                                                           >  1:2543716/1‑148 (MQ=255)
                                                                                     ttCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGt                                                           >  1:2156501/1‑148 (MQ=255)
                                                                                         tCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTAt                                                       >  1:2061330/1‑148 (MQ=255)
                                                                                           ttCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCg                                                     >  2:1527371/1‑148 (MQ=255)
                                                                                             cATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGcc                                                   <  1:2118493/148‑1 (MQ=255)
                                                                                              aTAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGccc                                                  <  1:2610041/148‑1 (MQ=255)
                                                                                              aTAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGccc                                                  <  1:1681863/148‑1 (MQ=255)
                                                                                               tAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCt                                                 >  1:1876656/1‑148 (MQ=255)
                                                                                                aGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCtt                                                >  1:3415992/1‑148 (MQ=255)
                                                                                                   tatCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAAt                                             <  1:506781/148‑1 (MQ=255)
                                                                                                    atCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAAtt                                            >  1:3055744/1‑148 (MQ=255)
                                                                                                     tCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTc                                           >  2:833503/1‑148 (MQ=255)
                                                                                                          cGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCt                                      >  1:2424843/1‑148 (MQ=255)
                                                                                                          cGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCt                                      <  2:686629/148‑1 (MQ=255)
                                                                                                            cAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCttt                                    >  1:1448483/1‑148 (MQ=255)
                                                                                                              ggCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTgg                                  <  1:1956740/148‑1 (MQ=255)
                                                                                                              ggCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTACCCCTATgg                                  >  1:1877597/1‑148 (MQ=255)
                                                                                                               gCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGc                                 <  2:2982299/148‑1 (MQ=255)
                                                                                                               gCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGc                                 >  1:2524100/1‑148 (MQ=255)
                                                                                                               gCTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGc                                 >  2:3138728/1‑148 (MQ=255)
                                                                                                                cTGGGTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCa                                <  1:3167891/148‑1 (MQ=255)
                                                                                                                   ggTAGAAGAAGAACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTa                             >  1:2180582/1‑148 (MQ=255)
                                                                                                                      agaagaagaACACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATg                          >  1:60883/1‑148 (MQ=255)
                                                                                                                       gaagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGt                         <  2:445901/148‑1 (MQ=255)
                                                                                                                       gaagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGt                         <  1:2256309/148‑1 (MQ=255)
                                                                                                                       gaagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATCTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGt                         <  2:837633/148‑1 (MQ=255)
                                                                                                                        aagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCTCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAAACAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTc                        <  2:2262748/148‑1 (MQ=255)
                                                                                                                        aagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTcc                                          >  1:1110045/1‑130 (MQ=255)
                                                                                                                        aagaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTcc                                          <  2:1110045/130‑1 (MQ=255)
                                                                                                                         agaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCt                       <  1:3442569/148‑1 (MQ=255)
                                                                                                                         agaagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCt                       >  2:1670227/1‑148 (MQ=255)
                                                                                                                           aagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCttt                     >  1:1029261/1‑148 (MQ=255)
                                                                                                                           aagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCttt                     >  2:1046759/1‑148 (MQ=255)
                                                                                                                           aagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCttt                     >  2:2430853/1‑148 (MQ=255)
                                                                                                                           aagaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCttt                     >  1:3257220/1‑148 (MQ=255)
                                                                                                                           aagaaCACAGATGACCAATAACGTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCttt                     <  2:2442708/148‑1 (MQ=255)
                                                                                                                             gaaCACAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTaa                   >  2:490185/1‑148 (MQ=255)
                                                                                                                               acacAGATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAAtt                 >  2:2524424/1‑148 (MQ=255)
                                                                                                                                    gATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTg            >  2:2104664/1‑148 (MQ=255)
                                                                                                                                    gATGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTg            <  2:1767946/148‑1 (MQ=255)
                                                                                                                                     aTGACCAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGa           >  1:3396081/1‑148 (MQ=255)
                                                                                                                                         ccAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCt                       <  1:1797758/132‑1 (MQ=255)
                                                                                                                                          cAATAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGAcgcgc      >  1:311964/1‑148 (MQ=255)
                                                                                                                                            aTAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACgcgcgc    >  1:2811700/1‑148 (MQ=255)
                                                                                                                                            aTAACTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACgcgcgc    >  2:926266/1‑148 (MQ=255)
                                                                                                                                              aaCTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACGCGCGCCg  <  1:2208431/148‑1 (MQ=255)
                                                                                                                                              aaCTTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACGCGCGCCg  <  1:1517810/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CCAGCCCGGCAGTACGCATAATGTGTTGATAACCGCGTTGGGTCAGCTCCGGGTTGGTCGCTCCCGGCGAGATCATCAGAATACCTTCGTCTTCATAGATATCTGACGCAGGCTGGGTAGAAGAAGAACACAGATGACCAATAACGTATTTAATGCCGTCATTAACGATTTTGTTGGCGACCGCAACGGCTTGTTTCGGGTCGCATGCGTCGTCATATTCCACGCCAACCAGTTTATCGCCCTTAATTCCCCCTTTGGCATTAATGTCTTTAATTGCCTGACGCGCGCCG  >  CP009273/3590491‑3590780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑