Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,591,957 T→G 100% V354V (GTA→GTC)  livJ ← leucine/isoleucine/valine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,591,9570TG98.6% 841.6 / ‑2.0 206V354V (GTA→GTC) livJleucine/isoleucine/valine transporter subunit
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base G (103/100);  total (104/102)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 206 total aligned reads displayed.

TTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAG  >  CP009273/3591812‑3592100
                                                                                                                                                 |                                                                                                                                               
 ttGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACg                                                                                                                                              >  2:1069398/1‑148 (MQ=255)
   ggATCTTGAAGTACTGATAATCCGTTAACACCGCATCCGGCATGAAGCAACGTACTAGATATTAGAAATTTCGAGGCAACCCAAAGTAGACGATAAATGATTACTTCGCAACGGTCTCCGTGCCGTTGCCGATCCAGTAAAAGACTcc                                                                                                                                            >  2:770962/1‑148 (MQ=255)
     aTCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGa                                                                                                                                          >  1:1831349/1‑148 (MQ=255)
      tCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGaa                                                                                                                                         <  1:3282584/148‑1 (MQ=255)
           tAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCaa                                                                                                                                    >  2:3190145/1‑148 (MQ=255)
             ggCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAg                                                                                                                                  <  2:625435/148‑1 (MQ=255)
             ggCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAg                                                                                                                                  <  2:2387318/148‑1 (MQ=255)
              gCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGc                                                                                                                                 >  1:2412265/1‑148 (MQ=255)
               ccGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGcc                                                                                                                                <  1:2903720/148‑1 (MQ=255)
               ccGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGcc                                                                                                                                <  1:3039553/148‑1 (MQ=255)
               ccGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGcc                                                                                                                                >  1:967161/1‑148 (MQ=255)
                  gATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCttt                                                                                                                             >  2:3302167/1‑148 (MQ=255)
                  gATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCttt                                                                                                                             <  1:270699/148‑1 (MQ=255)
                  gATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCttt                                                                                                                             >  2:3127145/1‑148 (MQ=255)
                   aTAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTc                                                                                                                            >  1:1424003/1‑148 (MQ=255)
                   aTAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTc                                                                                                                            >  1:2445148/1‑148 (MQ=255)
                    tAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCa                                                                                                                           >  2:2129328/1‑148 (MQ=255)
                    tAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCa                                                                                                                           >  2:3041905/1‑148 (MQ=255)
                    tAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCa                                                                                                                           >  2:364117/1‑148 (MQ=255)
                           tttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGcc                                                                                                                    >  2:3459932/1‑148 (MQ=255)
                           tttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGcc                                                                                                                    >  2:2993212/1‑148 (MQ=255)
                           tttACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGcc                                                                                                                    >  2:2891130/1‑148 (MQ=255)
                                 ccgcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTtctc                                                                                                              <  1:253423/148‑1 (MQ=255)
                                 ccgcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTtctc                                                                                                              <  2:935424/148‑1 (MQ=255)
                                   gcATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCAt                                                                                                            <  1:1641910/148‑1 (MQ=255)
                                     aTCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATcc                                                                                                          <  1:1767680/148‑1 (MQ=255)
                                     aTCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATcc                                                                                                          <  1:1765477/148‑1 (MQ=255)
                                     aTCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATcc                                                                                                          <  2:2116972/148‑1 (MQ=255)
                                      tCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATccc                                                                                                         >  1:749464/1‑148 (MQ=255)
                                         ggCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAgg                                                                                                      <  2:3025815/148‑1 (MQ=255)
                                          gCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGt                                                                                                     <  1:928900/148‑1 (MQ=255)
                                          gCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGt                                                                                                     >  1:165721/1‑148 (MQ=255)
                                             tGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAg                                                                                                  <  1:633490/148‑1 (MQ=255)
                                               aaGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCg                                                                                                >  1:2220913/1‑148 (MQ=255)
                                                aGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCgg                                                                                               >  1:335077/1‑148 (MQ=255)
                                                    aCGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTccc                                                                                           <  1:2206480/148‑1 (MQ=255)
                                                     cGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCa                                                                                          >  2:2024247/1‑148 (MQ=255)
                                                        aCTCGATATTCGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTa                                                                                       <  2:2509253/148‑1 (MQ=255)
                                                        aCTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTa                                                                                       >  1:889508/1‑148 (MQ=255)
                                                        aCTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTa                                                                                       >  2:1788782/1‑148 (MQ=255)
                                                         cTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTAc                                                                                      >  1:763180/1‑148 (MQ=255)
                                                           cGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACgg                                                                                    <  1:2807849/148‑1 (MQ=255)
                                                           cGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACgg                                                                                    >  1:1575952/1‑148 (MQ=255)
                                                           cGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACgg                                                                                    <  1:2350042/148‑1 (MQ=255)
                                                            gATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGt                                                                                   <  2:871426/148‑1 (MQ=255)
                                                             atatTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTa                                                                                  >  1:2693464/1‑148 (MQ=255)
                                                               atTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATc                                                                                >  1:1011334/1‑148 (MQ=255)
                                                                ttAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATcc                                                                               >  1:1413374/1‑148 (MQ=255)
                                                                ttAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATcc                                                                               <  2:1169233/148‑1 (MQ=255)
                                                                 tAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCa                                                                              <  1:1653541/148‑1 (MQ=255)
                                                                 tAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCa                                                                              <  1:992298/148‑1 (MQ=255)
                                                                 tAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCa                                                                              >  1:764967/1‑148 (MQ=255)
                                                                  aGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCAc                                                                             >  2:2448387/1‑148 (MQ=255)
                                                                      aTTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAg                                                                         <  2:286255/148‑1 (MQ=255)
                                                                          ggcggcAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCg                                                                     >  2:781080/1‑148 (MQ=255)
                                                                              gcAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCttt                                                                 <  1:1046360/148‑1 (MQ=255)
                                                                              gcAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCttt                                                                 >  1:3349119/1‑148 (MQ=255)
                                                                                     aaaGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAt                                                          >  1:2292714/1‑148 (MQ=255)
                                                                                     aaaGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAt                                                          >  1:796296/1‑148 (MQ=255)
                                                                                        gTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTg                                                       <  2:1424003/148‑1 (MQ=255)
                                                                                         ttGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTgg                                                      >  2:942862/1‑148 (MQ=255)
                                                                                             cGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGAt                                                  <  2:660157/148‑1 (MQ=255)
                                                                                               aTTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGAttt                                                <  1:426120/148‑1 (MQ=255)
                                                                                               aTTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGAttt                                                <  2:1527527/148‑1 (MQ=255)
                                                                                                 tAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCa                                              >  1:1032318/1‑148 (MQ=255)
                                                                                                  aaTGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAg                                             <  1:1254595/148‑1 (MQ=255)
                                                                                                    tGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGcc                                           <  1:2788358/148‑1 (MQ=255)
                                                                                                     gATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCg                                          >  2:2834363/1‑148 (MQ=255)
                                                                                                       ttACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                        >  2:493074/1‑148 (MQ=255)
                                                                                                       ttACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGa                                        >  1:2775764/1‑148 (MQ=255)
                                                                                                        tACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGAt                                       <  2:2120120/148‑1 (MQ=255)
                                                                                                        tACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGAt                                       >  1:158495/1‑148 (MQ=255)
                                                                                                        tACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGAt                                       <  1:3041086/148‑1 (MQ=255)
                                                                                                          cTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCg                                     >  1:3332411/1‑148 (MQ=255)
                                                                                                            tCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCACCGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                   <  2:1358185/148‑1 (MQ=255)
                                                                                                            tCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                   <  2:1935662/148‑1 (MQ=255)
                                                                                                            tCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTc                                   <  2:536030/148‑1 (MQ=255)
                                                                                                              gCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAg                                 <  1:196126/148‑1 (MQ=255)
                                                                                                                 tCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACt                              >  2:2783903/1‑148 (MQ=255)
                                                                                                                  cGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTg                             >  1:3270897/1‑148 (MQ=255)
                                                                                                                  cGGTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTg                             >  1:2637767/1‑148 (MQ=255)
                                                                                                                   ggTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                            <  2:2445148/148‑1 (MQ=255)
                                                                                                                   ggTCGCCGTGCCGTTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGa                            <  1:1162253/148‑1 (MQ=255)
                                                                                                                        ccgtgccgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAg                       >  2:2540087/1‑148 (MQ=255)
                                                                                                                        ccgtgccgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTAGTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAg                       >  2:585960/1‑148 (MQ=255)
                                                                                                                           tgccgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGTTCGTCAGACTGATTCAGGcc                    >  2:1588973/1‑148 (MQ=255)
                                                                                                                           tgccgtTGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGcc                    >  2:1589187/1‑148 (MQ=255)
                                                                                                                                 tGGCGTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTg              >  2:3386979/1‑148 (MQ=255)
                                                                                                                                     gTGCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCaaa          <  1:1874009/148‑1 (MQ=255)
                                                                                                                                       gCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa        <  1:2372260/148‑1 (MQ=255)
                                                                                                                                       gCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa        <  1:2129328/148‑1 (MQ=255)
                                                                                                                                       gCCAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGa        <  1:364117/148‑1 (MQ=255)
                                                                                                                                        ccAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAt       <  2:898136/148‑1 (MQ=255)
                                                                                                                                        ccAGTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGAt       <  2:165721/148‑1 (MQ=255)
                                                                                                                                           gTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGc    <  2:1861362/148‑1 (MQ=255)
                                                                                                                                           gTCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGc    <  1:942905/148‑1 (MQ=255)
                                                                                                                                            tCAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCa   >  2:2291485/1‑148 (MQ=255)
                                                                                                                                             cAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAg  <  1:2602804/148‑1 (MQ=255)
                                                                                                                                             cAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAg  <  2:1971093/148‑1 (MQ=255)
                                                                                                                                             cAAAGACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAg  <  2:334889/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TTTGGATCTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATGAAGCAACGTACTCGATATTAGCAATTTGGCGGCAACCCAAAGTTGCCGATTAATGATTACTTCGCATCGGTCGCCGTGCCGTTGGCGTGCCAGTCAAATACGCCGAACTCAAAGCCTTTCAGATCGCCTTTCTCATCCCAGGTCAGCGGTCCCATTACGGTATCCACGGAGTTCGCTTTCAGGTATTTGGCGATTTCAGCCGGATCGTCAGACTGATTCAGGCCCGCCTGCAAAGATTGCAG  >  CP009273/3591812‑3592100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑