Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,596,266 C→A 100% T446T (ACG→ACT)  ftsY ← Signal Recognition Particle (SRP) receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,596,2660CA99.6% 746.0 / ‑4.3 226T446T (ACG→ACT) ftsYSignal Recognition Particle (SRP) receptor
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (123/102);  total (123/103)
Fisher's exact test for biased strand distribution p-value = 4.56e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 228 total aligned reads displayed.

GGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTC  >  CP009273/3596122‑3596410
                                                                                                                                                |                                                                                                                                                
ggCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGtt                                                                                                                                               >  1:766929/1‑148 (MQ=255)
  cAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTttagttag                                                                                                                                             >  2:3133271/1‑148 (MQ=255)
  cAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTttagttag                                                                                                                                             >  2:3236729/1‑148 (MQ=255)
     aaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGt                                                                                                                                          >  1:1057139/1‑148 (MQ=255)
     aaaGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGt                                                                                                                                          >  1:1056281/1‑148 (MQ=255)
       aGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGa                                                                                                                                        >  2:869533/1‑148 (MQ=255)
        gTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGAt                                                                                                                                       >  1:1679708/1‑148 (MQ=255)
         tGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATg                                                                                                                                      >  1:2812744/1‑148 (MQ=255)
           ccTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGcc                                                                                                                                    >  1:1266311/1‑148 (MQ=255)
           ccTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGcc                                                                                                                                    >  1:3132526/1‑148 (MQ=255)
           ccTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGcc                                                                                                                                    >  1:297193/1‑148 (MQ=255)
           ccTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGcc                                                                                                                                    >  2:3501665/1‑148 (MQ=255)
                ataAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTa                                                                                                                               >  1:2355655/1‑148 (MQ=255)
                  aaaGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAg                                                                                                                             >  2:686725/1‑148 (MQ=255)
                   aaGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGc                                                                                                                            >  1:2906352/1‑148 (MQ=255)
                    aGTCGTCCGCCTTAAACGGACTCAAATCCACAATAAGTTCTCAGACACAAAAGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTACGCCGAGCAGACCAGTTTAGTTAGCTTCATGCCCGTTAAgac                                                                                                                           >  2:3046003/1‑146 (MQ=255)
                         tcCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACgg                                                                                                                      <  2:2085594/148‑1 (MQ=255)
                          ccGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGc                                                                                                                     >  2:2566306/1‑148 (MQ=255)
                          ccGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGc                                                                                                                     >  1:2781473/1‑148 (MQ=255)
                           cGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCt                                                                                                                    >  2:2601124/1‑148 (MQ=255)
                           cGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCt                                                                                                                    >  2:843589/1‑148 (MQ=255)
                            gCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCtt                                                                                                                   >  2:2891323/1‑148 (MQ=255)
                              cTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCa                                                                                                                 <  1:2111614/148‑1 (MQ=255)
                                   aCGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGaa                                                                                                            >  1:1907452/1‑148 (MQ=255)
                                    cGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAAc                                                                                                           <  1:494167/148‑1 (MQ=255)
                                      gACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAg                                                                                                         <  1:241130/148‑1 (MQ=255)
                                       aCGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGt                                                                                                        >  1:2285591/1‑148 (MQ=255)
                                       aCGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGt                                                                                                        <  2:1554197/148‑1 (MQ=255)
                                       aCGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGt                                                                                                        >  2:181185/1‑148 (MQ=255)
                                       aCGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGt                                                                                                        <  2:1554161/148‑1 (MQ=255)
                                       aCGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGt                                                                                                        >  2:2323038/1‑148 (MQ=255)
                                       aCGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGt                                                                                                        >  1:1486210/1‑148 (MQ=255)
                                       aCGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGt                                                                                                        >  2:1222312/1‑148 (MQ=255)
                                          cAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTg                                                                                                     >  1:2572498/1‑148 (MQ=255)
                                           aaaTCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTgg                                                                                                    <  1:145849/148‑1 (MQ=255)
                                              tCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCt                                                                                                 >  2:536216/1‑148 (MQ=255)
                                               ccTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTg                                                                                                >  1:1375432/1‑148 (MQ=255)
                                               ccTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTg                                                                                                >  1:2402217/1‑148 (MQ=255)
                                                 tCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGc                                                                                              <  2:3110866/148‑1 (MQ=255)
                                                 tCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGc                                                                                              >  1:3299045/1‑148 (MQ=255)
                                                 tCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGc                                                                                              >  1:2489138/1‑148 (MQ=255)
                                                    aTACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTa                                                                                           <  2:172138/148‑1 (MQ=255)
                                                      aCGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGATTGGCCTGGCTTAcc                                                                                         >  2:1023881/1‑148 (MQ=255)
                                                         ttCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACcgcg                                                                                      <  2:271570/148‑1 (MQ=255)
                                                          tCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGt                                                                                     <  1:169855/148‑1 (MQ=255)
                                                           cGCCGACACCACTGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGtt                                                                                    <  2:2216746/148‑1 (MQ=255)
                                                           cGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGtt                                                                                    <  2:622782/148‑1 (MQ=255)
                                                            gCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTc                                                                                   <  2:2400165/148‑1 (MQ=255)
                                                              cGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTg                                                                                 >  1:2120755/1‑148 (MQ=255)
                                                                acacCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGcc                                                                               >  2:554646/1‑148 (MQ=255)
                                                                  acCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCg                                                                             <  2:1490121/148‑1 (MQ=255)
                                                                   ccAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCgg                                                                            >  2:2010639/1‑148 (MQ=255)
                                                                    cAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGt                                                                           >  2:916510/1‑148 (MQ=255)
                                                                      aTGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGc                                                                         <  1:664091/148‑1 (MQ=255)
                                                                       tGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCt                                                                        >  1:934429/1‑148 (MQ=255)
                                                                         tAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTgg                                                                      <  2:1057139/148‑1 (MQ=255)
                                                                          aGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGc                                                                     <  2:3172222/148‑1 (MQ=255)
                                                                              gATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCa                                                                 <  2:3479440/148‑1 (MQ=255)
                                                                                tAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAAt                                                               <  1:350373/148‑1 (MQ=255)
                                                                                  gggATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCCACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAg                                                             >  1:26567/1‑148 (MQ=255)
                                                                                   ggATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGTCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGt                                                            <  2:145832/148‑1 (MQ=255)
                                                                                   ggATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGt                                                            <  1:207047/148‑1 (MQ=255)
                                                                                      tACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAg                                                         >  1:1141716/1‑148 (MQ=255)
                                                                                         cAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCAt                                                      >  1:2860477/1‑148 (MQ=255)
                                                                                           aaCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATaa                                                    <  1:1463777/148‑1 (MQ=255)
                                                                                           aaCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATaa                                                    >  1:73319/1‑148 (MQ=255)
                                                                                            aCTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAAc                                                   <  2:1873971/148‑1 (MQ=255)
                                                                                             cTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACt                                                  >  1:2598286/1‑148 (MQ=255)
                                                                                              tGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACtt                                                 <  2:1037335/148‑1 (MQ=255)
                                                                                               ggTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTc                                                <  1:1973545/148‑1 (MQ=255)
                                                                                               ggTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTc                                                <  1:2694118/148‑1 (MQ=255)
                                                                                                  cAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATg                                             <  1:181185/148‑1 (MQ=255)
                                                                                                  cAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATg                                             <  1:1222312/148‑1 (MQ=255)
                                                                                                   aGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATgc                                            <  1:3236729/148‑1 (MQ=255)
                                                                                                    gCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATgcg                                           >  2:1747969/1‑148 (MQ=255)
                                                                                                       aCCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATgcggcg                                        >  2:2186364/1‑148 (MQ=255)
                                                                                                       aCCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATgcggcg                                        <  1:830404/148‑1 (MQ=255)
                                                                                                        ccGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGgcgc                                       <  1:3408862/148‑1 (MQ=255)
                                                                                                        ccGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGgcgc                                       <  1:1332345/148‑1 (MQ=255)
                                                                                                          gagaAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCtt                                     <  1:3028372/148‑1 (MQ=255)
                                                                                                           agaAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTc                                    >  2:2852179/1‑148 (MQ=255)
                                                                                                                aTTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGt                               >  1:1084095/1‑148 (MQ=255)
                                                                                                                aTTACCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGt                               <  2:254441/148‑1 (MQ=255)
                                                                                                                   aCCCCGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGa                            <  1:3181652/148‑1 (MQ=255)
                                                                                                                      ccGCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGtt                         <  1:2548120/148‑1 (MQ=255)
                                                                                                                        gCCTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttc                       <  1:3459652/148‑1 (MQ=255)
                                                                                                                         ccTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttct                      <  2:1260607/148‑1 (MQ=255)
                                                                                                                          cTTTCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttctt                     <  1:1769615/148‑1 (MQ=255)
                                                                                                                           tttCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTttcttc                    <  2:2992445/148‑1 (MQ=255)
                                                                                                                            ttCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtca                   >  1:2272856/1‑148 (MQ=255)
                                                                                                                            ttCGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtca                   <  1:456127/148‑1 (MQ=255)
                                                                                                                              cGCCGTGCCGTCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcatc                 <  1:567790/148‑1 (MQ=255)
                                                                                                                               gccgtgccgtCCAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTtcatca                >  2:1049716/1‑148 (MQ=255)
                                                                                                                                         ccAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt      >  1:1901915/1‑148 (MQ=255)
                                                                                                                                         ccAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt      >  1:1902016/1‑148 (MQ=255)
                                                                                                                                         ccAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt      >  2:1391113/1‑148 (MQ=255)
                                                                                                                                         ccAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAt      >  2:1961643/1‑148 (MQ=255)
                                                                                                                                          cAGTTTAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGAtt     >  2:1835101/1‑148 (MQ=255)
                                                                                                                                             tttAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttc  <  1:3499941/148‑1 (MQ=255)
                                                                                                                                             tttAGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTttc  <  2:1506499/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GGCAAAAAGTGCCTCTATAAAGTCGTCCGCCTTAAACGGACGCAAATCCTCAATACGTTCGCCGACACCAATGTAGCGGATAGGGATACCAAACTGGTCAGCCACCGAGAAAATTACCCCGCCTTTCGCCGTGCCGTCCAGTTTCGTTAGCGTGATGCCGGTTAAGCCAACGGCTTCATGGAACAGTTTGGCCTGGCTTACCGCGTTCTGCCCGGTGCTGGCATCAATAGTCAGCATAACTTCATGCGGCGCTTCAACGTCGAGTTTCTTCATCACGCGGACGATTTTC  >  CP009273/3596122‑3596410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑