Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,598,440 C→A 100% N34K (AAC→AAA)  yhhL → DUF1145 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,598,4400CA98.4% 595.0 / 0.6 185N34K (AAC→AAA) yhhLDUF1145 family protein
Reads supporting (aligned to +/- strand):  ref base C (3/0);  new base A (109/73);  total (112/73)
Fisher's exact test for biased strand distribution p-value = 2.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 185 total aligned reads displayed.

GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAG  >  CP009273/3598295‑3598584
                                                                                                                                                 |                                                                                                                                                
 gTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTg                                                                                                                                               <  1:1008475/148‑1 (MQ=255)
   ggCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGgc                                                                                                                                             >  2:512088/1‑148 (MQ=255)
     cTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGgcgc                                                                                                                                           >  1:158877/1‑148 (MQ=255)
         tCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGAt                                                                                                                                       <  1:3477288/148‑1 (MQ=255)
           ggCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGAttt                                                                                                                                     <  1:2304060/148‑1 (MQ=255)
            gCTGTATCAACGCGAAGCACAAGGAGAAAGTGCTGCTGATTAATATTTGTCGTGTGTTAATGATCTGCGTTTGGGGATTTTTAAACCTAAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATATTCGTTAAATTTGCGCTGTtttt                                                                                                                                    >  1:3274031/1‑148 (MQ=255)
              tGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTa                                                                                                                                  <  1:1992750/148‑1 (MQ=255)
              tGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTa                                                                                                                                  >  2:1832372/1‑148 (MQ=255)
               gTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGAttttttc                                                                                                                                 <  1:3002453/148‑3 (MQ=255)
                  tCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGt                                                                                                                              >  1:1856680/1‑148 (MQ=255)
                  tCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGt                                                                                                                              >  1:2407023/1‑148 (MQ=255)
                    aaCGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGc                                                                                                                            <  2:3082164/148‑1 (MQ=255)
                      cgcgAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTg                                                                                                                          <  1:2881085/148‑1 (MQ=255)
                         gAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATg                                                                                                                       <  2:3169734/148‑1 (MQ=255)
                          aaGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGc                                                                                                                      <  1:2102356/148‑1 (MQ=255)
                          aaGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGc                                                                                                                      <  2:1462465/148‑1 (MQ=255)
                          aaGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGc                                                                                                                      <  1:672896/148‑1 (MQ=255)
                           aGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCa                                                                                                                     >  2:368430/1‑148 (MQ=255)
                            gCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCAt                                                                                                                    >  1:571984/1‑148 (MQ=255)
                            gCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCAt                                                                                                                    >  1:3452177/1‑148 (MQ=255)
                             cacaAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATg                                                                                                                   >  1:2862236/1‑148 (MQ=255)
                             cacaAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATg                                                                                                                   >  2:1770929/1‑148 (MQ=255)
                             cacaAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATg                                                                                                                   >  1:1654921/1‑148 (MQ=255)
                              acaAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATgg                                                                                                                  <  2:1757442/148‑1 (MQ=255)
                                aaGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTa                                                                                                                <  1:509590/148‑1 (MQ=255)
                                 aGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTAt                                                                                                               >  1:109822/1‑148 (MQ=255)
                                   gagaAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGc                                                                                                             <  1:1423759/148‑1 (MQ=255)
                                      aaaGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGc                                                                                                          <  2:3406865/148‑1 (MQ=255)
                                         gTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTgg                                                                                                       <  2:1598553/148‑1 (MQ=255)
                                         gTGATGCTGATGAATCTTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTgg                                                                                                       <  2:3178963/148‑1 (MQ=255)
                                            aTGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAAACTGGTGCATCCCTTACCACCCCCGCTGAATATCATCGTTAAAGTGGCGCTGATTTTTAACGTGCTGATGCAAGGTATGCATCTGgcgc                                                                                                    >  2:1491554/1‑148 (MQ=255)
                                             tGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCt                                                                                                   >  1:761598/1‑148 (MQ=255)
                                               cTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTAt                                                                                                 <  2:3470923/148‑1 (MQ=255)
                                                   ttAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATACTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTAGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGaa                                                                                             >  2:1190449/1‑148 (MQ=255)
                                                    tAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGaaa                                                                                            >  1:2057395/1‑148 (MQ=255)
                                                    tAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTCCCCACGCCCGCTGAATATATTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGaaa                                                                                            >  1:2057433/1‑148 (MQ=255)
                                                    tAATATTGGTCGTTTGTTAAAGCTCTGCGTTTGGGGATTTTTAATACTCAACCTGGTGCAACCCTTACCACGCCCGCTGAATATCTTAGTTAAATTTGCGCAGATTTTTTCAGTGCTGATTCATGTTATGCAGCTGGCGCTATTGaaa                                                                                            >  1:1829790/1‑148 (MQ=255)
                                                      atatTGGTCGTTTTTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTAACGTGCTGATGCATGGTATTCAGCTGGCGCTATTGAAATc                                                                                          >  1:1299164/1‑148 (MQ=255)
                                                      atatTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATc                                                                                          <  2:1324654/148‑1 (MQ=255)
                                                      atatTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATc                                                                                          >  2:654866/1‑148 (MQ=255)
                                                       tatTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATcc                                                                                         <  2:323902/148‑1 (MQ=255)
                                                        atTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGTTGCATGGTATGCAGCTGGCGCTATTGAAATACa                                                                                        >  2:2960249/1‑148 (MQ=255)
                                                        atTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCa                                                                                        >  2:421614/1‑148 (MQ=255)
                                                        atTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATATTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCa                                                                                        >  1:2134268/1‑148 (MQ=255)
                                                          tGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACt                                                                                      <  1:918939/148‑1 (MQ=255)
                                                              cGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTAc                                                                                  <  1:1246851/148‑1 (MQ=255)
                                                              cGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTGTAc                                                                                  >  2:3314484/1‑148 (MQ=255)
                                                                 ttGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCCTCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGa                                                                               >  1:681890/1‑148 (MQ=255)
                                                                 ttGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTTATGCATGGTATGCAGCGGGCGCGATTGAAATCCACTTTACCGa                                                                               >  1:362496/1‑148 (MQ=255)
                                                                 ttGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGa                                                                               >  1:3502792/1‑148 (MQ=255)
                                                                  tgagaaagCTCGGCGTTTGGGGATTGATACTCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGaa                                                                              <  2:2791003/141‑1 (MQ=255)
                                                                       aTGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGTAATCCACGTTACCGAAAGATg                                                                         >  1:2939675/1‑148 (MQ=255)
                                                                         gCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGc                                                                       >  2:971544/1‑148 (MQ=255)
                                                                            ctGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCg                                                                    >  2:564693/1‑148 (MQ=255)
                                                                             tGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGc                                                                   >  1:3089947/1‑148 (MQ=255)
                                                                                 tttGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGAt                                                               >  2:200161/1‑148 (MQ=255)
                                                                                    ggggATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGac                                                            <  2:2902960/148‑1 (MQ=255)
                                                                                         tttttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCAccg                                                       >  2:2003314/1‑148 (MQ=255)
                                                                                           tttAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTCTGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCAccgcc                                                     >  2:1889034/1‑148 (MQ=255)
                                                                                             tAATCCTCAACCTGGTGCATCCCGTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGa                                                   <  2:264426/148‑1 (MQ=255)
                                                                                               aTCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGaaa                                                 >  1:1238668/1‑148 (MQ=255)
                                                                                                   tCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGt                                             >  1:941808/1‑148 (MQ=255)
                                                                                                   tCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGt                                             >  1:941800/1‑148 (MQ=255)
                                                                                                   tCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGAGTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTAGTGAAATCCCGTTTAGCGCACGTTGGCCCGCCGATGACCACCGCCGAAAgggg                                             >  2:2238235/1‑147 (MQ=255)
                                                                                                    cAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTa                                            >  1:463395/1‑148 (MQ=255)
                                                                                                       ccTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTTGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACgg                                         >  1:1885904/1‑148 (MQ=255)
                                                                                                        cTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGa                                        <  2:1955446/148‑1 (MQ=255)
                                                                                                        cTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGa                                        <  2:1603774/148‑1 (MQ=255)
                                                                                                          ggTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGAtt                                      >  2:2161174/1‑148 (MQ=255)
                                                                                                           gTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGAttt                                     <  2:534496/148‑1 (MQ=255)
                                                                                                            tGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGAtttt                                    >  1:3111900/1‑148 (MQ=255)
                                                                                                            tGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGAtttt                                    <  1:2449506/148‑1 (MQ=255)
                                                                                                                 cccTTCCCACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCttt                               >  2:2724491/1‑148 (MQ=255)
                                                                                                                  ccTTCCCACGCCCGCTGAATATCTTCGATAAAGTGGCGCTGATTTTGACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCtttt                              <  2:1185137/148‑1 (MQ=255)
                                                                                                                       ccACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCg                         <  1:393164/148‑1 (MQ=255)
                                                                                                                       ccACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCg                         >  1:1671045/1‑148 (MQ=255)
                                                                                                                        cACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCgt                        >  2:133925/1‑148 (MQ=255)
                                                                                                                        cACGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCgt                        >  1:1671652/1‑148 (MQ=255)
                                                                                                                         aCGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCgtg                       <  1:1820494/148‑1 (MQ=255)
                                                                                                                          cGCCCGCTGTATATCTTCGTTAAAGTGGCGCTGTTTTTTACCGTGCTGATGCATGGTATGCAGCTGGCTCTTTTGATATCCACTTTAACGAAAGATGGCCCGCAGATGTCCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCgtgt                      <  2:582238/148‑1 (MQ=255)
                                                                                                                          cGCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCgtgt                      <  2:2115176/148‑1 (MQ=255)
                                                                                                                           gCCCGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGGGTGtt                     >  1:3357476/1‑148 (MQ=255)
                                                                                                                             ccGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGtgtgtg                   >  2:2608628/1‑148 (MQ=255)
                                                                                                                              cGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGa                  >  2:2464783/1‑148 (MQ=255)
                                                                                                                              cGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGa                  >  1:1744697/1‑148 (MQ=255)
                                                                                                                              cGCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAAACCACTTTAACGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTgt                  >  1:2570198/1‑147 (MQ=255)
                                                                                                                               gCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGaa                 >  1:290138/1‑148 (MQ=255)
                                                                                                                               gCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGaa                 <  2:2862358/148‑1 (MQ=255)
                                                                                                                               gCTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGaa                 <  2:1654921/148‑1 (MQ=255)
                                                                                                                                cTGAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAAc                >  2:2380522/1‑148 (MQ=255)
                                                                                                                                  gAATATCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGGTTGAActg              >  1:1918864/1‑148 (MQ=255)
                                                                                                                                     tatCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActgctg           >  1:1442573/1‑148 (MQ=255)
                                                                                                                                     tatCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActgctg           >  1:1442993/1‑148 (MQ=255)
                                                                                                                                     tatCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAActgcgg           >  1:2737694/1‑146 (MQ=255)
                                                                                                                                      atCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctgg          >  2:1596578/1‑148 (MQ=255)
                                                                                                                                       tCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGTACTGctggc         >  1:2412588/1‑148 (MQ=255)
                                                                                                                                       tCTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGGGTTTGAACTGctggc         >  1:2412577/1‑148 (MQ=255)
                                                                                                                                        cTTCGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcc        <  1:778664/148‑1 (MQ=255)
                                                                                                                                           cGTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctgg     <  1:1788017/148‑1 (MQ=255)
                                                                                                                                            gTTAAAGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGctggcctggc    <  1:972108/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTGGCTTATCGGCTGTATCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAATATTGGTCGTTTGTTAATGCTCTGCGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATATCTTCGTTAACGTGGCGCTGATTTTTACCGTGCTGATGCATGGTATGCAGCTGGCGCTATTGAAATCCACTTTACCGAAAGATGGCCCGCAGATGACCACCGCCGAAAAGGTACGGATTTTCCTTTTCGGCGTGTTTGAACTGCTGGCCTGGCAG  >  CP009273/3598295‑3598584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑