Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,607,110 C→A 100% I28I (ATC→ATA)  nikA → nickel‑binding, heme‑binding periplasmic protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,607,1100CA99.6% 767.0 / ‑4.4 231I28I (ATC→ATA) nikAnickel‑binding, heme‑binding periplasmic protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (115/115);  total (115/116)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 233 total aligned reads displayed.

ACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTG  >  CP009273/3606967‑3607253
                                                                                                                                               |                                                                                                                                               
aCGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATaacca                                                                                                                                             >  2:364730/1‑148 (MQ=255)
    aTACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGc                                                                                                                                         >  1:158922/1‑148 (MQ=255)
    aTACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGc                                                                                                                                         >  1:1011730/1‑148 (MQ=255)
    aTACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGc                                                                                                                                         <  2:396616/148‑1 (MQ=255)
     tACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                        <  2:2150327/148‑1 (MQ=255)
     tACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                        <  2:1161404/148‑1 (MQ=255)
     tACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                        <  2:3238773/148‑1 (MQ=255)
     tACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGcc                                                                                                                                        >  1:2453224/1‑148 (MQ=255)
      aCTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCt                                                                                                                                       >  1:573978/1‑148 (MQ=255)
         tAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGc                                                                                                                                    >  1:3302414/1‑148 (MQ=255)
            taTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCgg                                                                                                                                 <  2:2885852/147‑1 (MQ=255)
            aaTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCgg                                                                                                                                 <  1:1120755/148‑1 (MQ=255)
             aTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGt                                                                                                                                >  2:2309835/1‑148 (MQ=255)
             aTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGt                                                                                                                                >  2:1639425/1‑148 (MQ=255)
             aTCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGt                                                                                                                                >  2:2310617/1‑148 (MQ=255)
               cGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGa                                                                                                                              <  2:213632/148‑1 (MQ=255)
                 tCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAAt                                                                                                                            >  2:932897/1‑148 (MQ=255)
                  cATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATg                                                                                                                           <  2:714380/148‑1 (MQ=255)
                  cATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATg                                                                                                                           >  2:3071110/1‑148 (MQ=255)
                  cATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATg                                                                                                                           >  2:3071243/1‑148 (MQ=255)
                     aCTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCg                                                                                                                        <  1:2694622/148‑1 (MQ=255)
                     aCTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCg                                                                                                                        >  2:623736/1‑148 (MQ=255)
                      cTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCgg                                                                                                                       >  2:2886779/1‑148 (MQ=255)
                       ttATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCggg                                                                                                                      >  1:3221487/1‑148 (MQ=255)
                        tATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGc                                                                                                                     <  1:1774060/148‑1 (MQ=255)
                         aTTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGcc                                                                                                                    >  1:3011129/1‑148 (MQ=255)
                          tttCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCa                                                                                                                   >  2:2401481/1‑148 (MQ=255)
                          tttCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCa                                                                                                                   <  2:1887493/148‑1 (MQ=255)
                           ttCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCAc                                                                                                                  <  2:2438062/148‑1 (MQ=255)
                           ttCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCAc                                                                                                                  >  2:687263/1‑148 (MQ=255)
                              cGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTaa                                                                                                               >  1:664713/1‑148 (MQ=255)
                                 cATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAACTAACCACCGCCTCGCCGGTGAATGTCGGGCCCCTAAAcc                                                                                                            >  2:2073377/1‑148 (MQ=255)
                                 cATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAcc                                                                                                            <  1:170846/148‑1 (MQ=255)
                                 cATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAcc                                                                                                            <  2:1615342/148‑1 (MQ=255)
                                  aTCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAccc                                                                                                           >  1:3201344/1‑148 (MQ=255)
                                  aTCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAccc                                                                                                           >  2:811590/1‑148 (MQ=255)
                                   tCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCg                                                                                                          >  1:2066800/1‑148 (MQ=255)
                                    cTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGc                                                                                                         >  1:1149254/1‑148 (MQ=255)
                                     tATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCTTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCa                                                                                                        >  1:1218239/1‑148 (MQ=255)
                                        tttAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCt                                                                                                     <  1:488336/148‑1 (MQ=255)
                                          tAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCttt                                                                                                   <  2:3003186/148‑1 (MQ=255)
                                          tAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCttt                                                                                                   >  2:2436804/1‑148 (MQ=255)
                                          tAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCttt                                                                                                   >  1:2614407/1‑148 (MQ=255)
                                          tAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCttt                                                                                                   >  1:2355666/1‑148 (MQ=255)
                                                  tGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCt                                                                                           >  2:3378823/1‑148 (MQ=255)
                                                    gggTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTaa                                                                                         >  2:301160/1‑148 (MQ=255)
                                                       ttACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCt                                                                                                     <  1:2157968/133‑1 (MQ=255)
                                                        tACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAg                                                                                     >  1:3452391/1‑148 (MQ=255)
                                                        tACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAg                                                                                     <  2:664713/148‑1 (MQ=255)
                                                          ccATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGAt                                                                                   >  1:955441/1‑148 (MQ=255)
                                                            aTGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGt                                                                                 <  2:2136409/148‑1 (MQ=255)
                                                             tGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAAACCGCACCTTTACACGCCTAACCAGATGtt                                                                                >  1:386052/1‑148 (MQ=255)
                                                                 ctcCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGcc                                                                            >  2:666809/1‑148 (MQ=255)
                                                                   ccACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCa                                                                          >  1:1846611/1‑148 (MQ=255)
                                                                    cacaCTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCag                                                                         <  1:947920/148‑1 (MQ=255)
                                                                    cacaCTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCag                                                                         <  2:1682918/148‑1 (MQ=255)
                                                                      cacTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCagag                                                                       <  1:3184282/148‑1 (MQ=255)
                                                                        cTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCa                                                                     >  2:2583412/1‑148 (MQ=255)
                                                                        cTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCa                                                                     <  2:3155938/148‑1 (MQ=255)
                                                                        cTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCa                                                                     <  2:3226695/148‑1 (MQ=255)
                                                                        cTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCa                                                                     <  2:1305635/148‑1 (MQ=255)
                                                                              cgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGttt                                                               <  1:1068065/148‑1 (MQ=255)
                                                                              cgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGttt                                                               <  1:2842006/148‑1 (MQ=255)
                                                                              cgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGttt                                                               <  1:2513955/148‑1 (MQ=255)
                                                                              cgcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGttt                                                               <  2:561525/148‑1 (MQ=255)
                                                                               gcACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTa                                                              >  1:1966841/1‑148 (MQ=255)
                                                                                  ctctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGa                                                           <  2:1189014/148‑1 (MQ=255)
                                                                                   tctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGaa                                                          <  2:1011730/148‑1 (MQ=255)
                                                                                   tctATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGaa                                                          >  2:2647977/1‑148 (MQ=255)
                                                                                       tttGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCAt                                                      >  1:2849723/1‑148 (MQ=255)
                                                                                       tttGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCAt                                                      <  1:79251/148‑1 (MQ=255)
                                                                                       tttGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCAt                                                      <  1:673035/148‑1 (MQ=255)
                                                                                        ttGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCAtt                                                     <  2:3397392/148‑1 (MQ=255)
                                                                                         tGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTg                                                    <  2:425992/148‑1 (MQ=255)
                                                                                          gcgcTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTgg                                                   <  2:1823961/148‑1 (MQ=255)
                                                                                           cgcTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGt                                                  <  2:1009922/148‑1 (MQ=255)
                                                                                                 tgGATTGTGCGTATTTTATCGTACATGCCGATGAACCAGATGAATTAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAata                                            <  1:752817/148‑1 (MQ=255)
                                                                                                  ggCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAatat                                           <  1:3455341/148‑1 (MQ=255)
                                                                                                  ggCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTGACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAatat                                           <  2:67099/148‑1 (MQ=255)
                                                                                                   gCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATc                                          <  1:2612648/148‑1 (MQ=255)
                                                                                                   gCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATc                                          >  2:2060267/1‑148 (MQ=255)
                                                                                                   gCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATc                                          >  2:223159/1‑148 (MQ=255)
                                                                                                   gCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATc                                          <  2:2978943/148‑1 (MQ=255)
                                                                                                    cTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCa                                         <  1:1417358/148‑1 (MQ=255)
                                                                                                      tgtgCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAgg                                       >  1:274270/1‑148 (MQ=255)
                                                                                                            tCTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACg                                 <  2:3205099/148‑1 (MQ=255)
                                                                                                             cTTTTATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACgg                                <  1:3053906/148‑1 (MQ=255)
                                                                                                                ttATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGtgc                             >  2:2098686/1‑146 (MQ=255)
                                                                                                                ttATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTc                             <  1:851507/148‑1 (MQ=255)
                                                                                                                 tATCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCg                            >  2:153160/1‑148 (MQ=255)
                                                                                                                   tCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGt                          <  1:197811/148‑1 (MQ=255)
                                                                                                                   tCGTCCATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGt                          <  2:3310899/148‑1 (MQ=255)
                                                                                                                       ccATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATc                      >  2:1661299/1‑148 (MQ=255)
                                                                                                                       ccATGCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATc                      <  1:2030720/148‑1 (MQ=255)
                                                                                                                           gCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGt                  >  2:1202969/1‑148 (MQ=255)
                                                                                                                           gCCGCTGCACCAGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGt                  >  2:2869962/1‑148 (MQ=255)
                                                                                                                                      aGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaaaa       >  2:2691449/1‑148 (MQ=255)
                                                                                                                                      aGATGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCaaaa       <  1:3405856/148‑1 (MQ=255)
                                                                                                                                        aTGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAg     <  1:2142610/148‑1 (MQ=255)
                                                                                                                                        aTGAAATAACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAg     <  1:375037/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                               
ACGAATACTTAAAATCGTCATACTTATTTCCGCCATCTATTTTAATCCATTGGGGTTACCATGCTCTCCACACTCCGCCGCACTCTATTTGCGCTGCTGGCTTGTGCGTCTTTTATCGTCCATGCCGCTGCACCAGATGAAATCACCACCGCCTGGCCGGTGAATGTCGGGCCACTAAACCCGCACCTTTACACGCCTAACCAGATGTTCGCCCAGAGCATGGTTTATGAACCATTGGTGAAATATCAGGCAGACGGTTCGGTGATCCCGTGGCTGGCAAAAAGCTG  >  CP009273/3606967‑3607253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑