BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,649,460	T→A	93.1%	K65* (AAA→TAA)	hdeB ←	acid‑resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,649,460	0	T	A	93.1%	781.4 / 38.8	259	K65* (AAA→TAA)	hdeB	acid‑resistance protein
Reads supporting (aligned to +/- strand): ref base T (11/7); new base A (138/103); total (149/110)
Fisher's exact test for biased strand distribution p-value = 8.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.37e-01

Only 100 of 270 total aligned reads displayed.

CTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCG  >  CP009273/3649315‑3649603

cTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTaat                                                                                                                                               >  1:3100151/1‑148 (MQ=255)

  tGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTaatat                                                                                                                                             <  1:2142062/148‑1 (MQ=255)

  tGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTaatat                                                                                                                                             >  2:3272386/1‑148 (MQ=255)

  tGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTaatat                                                                                                                                             >  2:3003731/1‑148 (MQ=255)

  tGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTaatat                                                                                                                                             >  2:1254993/1‑148 (MQ=255)

  tGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGAAACTGTATCGCCACCTTaatat                                                                                                                                             >  2:2082466/1‑148 (MQ=255)

    cACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATAc                                                                                                                                           <  1:1925239/148‑1 (MQ=255)

      ccTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTg                                                                                                                                         >  1:472224/1‑148 (MQ=255)

      ccTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTg                                                                                                                                         >  1:854461/1‑148 (MQ=255)

           ttAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTttct                                                                                                                                    >  2:1314584/1‑148 (MQ=255)

            tAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTttctt                                                                                                                                   >  2:43820/1‑148 (MQ=255)

             aaTTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTttcttc                                                                                                                                  <  2:1697865/148‑1 (MQ=255)

                tCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATg                                                                                                                               >  1:226735/1‑148 (MQ=255)

                  ggCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATgca                                                                                                                             <  2:2863854/148‑1 (MQ=255)

                    cAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATgcagc                                                                                                                           >  2:2857595/1‑148 (MQ=255)

                    cAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATgcagc                                                                                                                           >  2:1106361/1‑148 (MQ=255)

                    cAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATgcagc                                                                                                                           >  2:628444/1‑148 (MQ=255)

                     aaGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATgcagca                                                                                                                          >  2:1445476/1‑148 (MQ=255)

                          aTTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATccac                                                                                                                     <  2:693881/148‑1 (MQ=255)

                          aTTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATccac                                                                                                                     <  1:2124678/148‑1 (MQ=255)

                          aTTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATccac                                                                                                                     >  2:19554/1‑148 (MQ=255)

                              gATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATg                                                                                                                 >  1:220197/1‑148 (MQ=255)

                                   ttGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTACTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATAGGTTTCATTTAAAGTAACGTTATAGCCCCCAAAAAATACTGTTTCTTCATGCAGCAACCACCAAGCAccc                                                                                                            >  1:1321542/1‑148 (MQ=255)

                                    tGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCg                                                                                                           <  1:1391771/148‑1 (MQ=255)

                                       tttttGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCgggg                                                                                                        <  1:3069348/148‑1 (MQ=255)

                                        ttttGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGt                                                                                                       >  2:1870581/1‑148 (MQ=255)

                                        ttttGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGt                                                                                                       <  1:2471364/148‑1 (MQ=255)

                                          ttGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCa                                                                                                     <  2:3169267/148‑1 (MQ=255)

                                          ttGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCa                                                                                                     <  1:2768125/148‑1 (MQ=255)

                                             aaGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTg                                                                                                  >  2:911658/1‑148 (MQ=255)

                                             aaGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTg                                                                                                  >  1:1665006/1‑148 (MQ=255)

                                               ggTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAAGATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCt                                                                                                >  1:3408745/1‑148 (MQ=255)

                                                gTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCtt                                                                                               <  2:1027785/148‑1 (MQ=255)

                                                   taCAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTg                                                                                            >  2:1349688/1‑148 (MQ=255)

                                                     cAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGa                                                                                          >  2:2970028/1‑148 (MQ=255)

                                                      aaaTTTTTCTGCGGGTTTTTCTTACATTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGAAGCATACACCATGCAACCGGGGTCATTGCTTTTGGAt                                                                                         >  1:2358427/1‑148 (MQ=255)

                                                        aTTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTc                                                                                       >  2:2406887/1‑148 (MQ=255)

                                                        aTTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTc                                                                                       <  2:3307913/148‑1 (MQ=255)

                                                        aTTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTc                                                                                       <  1:635619/148‑1 (MQ=255)

                                                        aTTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTGTTGGATTc                                                                                       >  2:2479036/1‑148 (MQ=255)

                                                         tttttCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCa                                                                                      >  1:3347623/1‑148 (MQ=255)

                                                          ttttCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAg                                                                                     <  1:288704/148‑1 (MQ=255)

                                                              cTGCGGGTTTTTCTTACAGTATTCGATCTCTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCa                                                                                 <  1:2323844/148‑1 (MQ=255)

                                                                gCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAAt                                                                               >  2:317301/1‑148 (MQ=255)

                                                                  gggTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATaa                                                                             <  2:1592271/148‑1 (MQ=255)

                                                                      ttttCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTc                                                                         <  1:3157290/148‑1 (MQ=255)

                                                                      ttttCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTc                                                                         <  1:1500136/148‑1 (MQ=255)

                                                                         tCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTg                                                                      <  2:620724/148‑1 (MQ=255)

                                                                              cAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAgg                                                                 >  2:1861541/1‑148 (MQ=255)

                                                                                gTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTc                                                               <  1:1713124/148‑1 (MQ=255)

                                                                                gTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTc                                                               <  2:2650767/148‑1 (MQ=255)

                                                                                  aTTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCat                                                             <  1:1055187/148‑1 (MQ=255)

                                                                                  aTTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCat                                                             <  2:358056/148‑1 (MQ=255)

                                                                                   ttCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCata                                                            <  2:478965/148‑1 (MQ=255)

                                                                                   ttCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCatt                                                            >  1:2933950/1‑147 (MQ=255)

                                                                                   ttCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCata                                                            <  2:136707/148‑1 (MQ=255)

                                                                                   ttCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCAATTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCata                                                            <  2:2244750/148‑1 (MQ=255)

                                                                                       aTCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTTATTCTTCATGCAGCATCCACCATGCAACCGGGGTCAGTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCtt                                                        >  1:3388558/1‑148 (MQ=255)

                                                                                        tCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCttt                                                       >  2:3315599/1‑148 (MQ=255)

                                                                                        tCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCttt                                                       >  1:1984374/1‑148 (MQ=255)

                                                                                           cTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGc                                                    >  1:1083653/1‑148 (MQ=255)

                                                                                            tttAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCg                                                   >  2:1190869/1‑148 (MQ=255)

                                                                                             ttAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCgg                                                  >  1:2448956/1‑148 (MQ=255)

                                                                                             ttAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCgg                                                  >  1:2449202/1‑148 (MQ=255)

                                                                                             ttAGGAATTTGAGTGAGATCGGTGTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCgg                                                  <  2:3088054/148‑1 (MQ=255)

                                                                                              tAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGa                                                 <  2:1054853/148‑1 (MQ=255)

                                                                                              tAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGa                                                 <  2:489164/148‑1 (MQ=255)

                                                                                                ggAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGAtt                                               <  1:409693/148‑1 (MQ=255)

                                                                                                 ggaTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTc                                              >  2:3128449/3‑148 (MQ=255)

                                                                                                 gAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTc                                              <  2:216337/148‑1 (MQ=255)

                                                                                                   aTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTAGCAGGTCATATCTTTAGCGGATTCAt                                            >  2:2277715/1‑148 (MQ=255)

                                                                                                   aTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCAt                                            >  1:770709/1‑148 (MQ=255)

                                                                                                   aTTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCAt                                            >  1:1249893/1‑148 (MQ=255)

                                                                                                    tttGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCAtt                                           >  2:183662/1‑148 (MQ=255)

                                                                                                     ttGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCTGGGTCATTGCTTTTGGATTCAGATCAATAAATTCATGGCAGGTCATATCTTTAGCGGATTCATTg                                          >  1:1209116/1‑148 (MQ=255)

                                                                                                     ttGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTg                                          <  2:1748733/148‑1 (MQ=255)

                                                                                                             gaTCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCaa                                  <  1:569769/148‑1 (MQ=255)

                                                                                                               tCGGTTTCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACg                                >  2:424783/1‑148 (MQ=255)

                                                                                                                   tttCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGa                            >  1:3253826/1‑148 (MQ=255)

                                                                                                                   tttCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGa                            >  1:2083895/1‑148 (MQ=255)

                                                                                                                   tttCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGa                            >  2:2883516/1‑148 (MQ=255)

                                                                                                                   tttCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGa                            >  2:3011480/1‑148 (MQ=255)

                                                                                                                   tttCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGa                            >  1:439186/1‑148 (MQ=255)

                                                                                                                     tCATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGAtt                          >  2:2756742/1‑148 (MQ=255)

                                                                                                                      cATTTAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATtg                         >  1:1052416/1‑148 (MQ=255)

                                                                                                                        tttAAAGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATtgtg                       >  1:276635/1‑148 (MQ=255)

                                                                                                                            aaGTAACGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGtt                   >  1:1301932/1‑148 (MQ=255)

                                                                                                                                 aCGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCa              <  2:2613721/148‑1 (MQ=255)

                                                                                                                                  cGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAg             >  2:1536553/1‑148 (MQ=255)

                                                                                                                                  cGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAg             >  2:791899/1‑148 (MQ=255)

                                                                                                                                  cGGTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAg             >  1:3289051/1‑148 (MQ=255)

                                                                                                                                    gTATCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTg           >  2:3428986/1‑148 (MQ=255)

                                                                                                                                       tCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACa        >  2:2728756/1‑148 (MQ=255)

                                                                                                                                       tCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACa        <  2:2146478/148‑1 (MQ=255)

                                                                                                                                       tCGCCACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACa        >  2:1733221/1‑148 (MQ=255)

                                                                                                                                         gCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACaaa      <  1:2436322/148‑1 (MQ=255)

                                                                                                                                          ccACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAg     <  2:2015314/148‑1 (MQ=255)

                                                                                                                                           cACCTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGc    >  1:3332543/1‑148 (MQ=255)

                                                                                                                                             ccTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCg  >  1:1743168/1‑148 (MQ=255)

                                                                                                                                             ccTTAATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCg  >  1:1871447/1‑148 (MQ=255)

CTTGCACCTCATTAATTCGGCAAGTCATTAGATGCTTGATTTTTGAAGGTATACAAATTTTTCTGCGGGTTTTTCTTACAGTATTCGATCACTTTAGGAATTTGAGTGAGATCGGTTTCATTTAAAGTAACGGTATCGCCACCTTTATATACTGTTTCTTCATGCAGCATCCACCATGCAACCGGGGTCATTGCTTTTGGATTCAGATCAATAAATTCCTGGCAGGTCATATCTTTAGCGGATTCATTGGCTGCCAACGCAGATTGTGCGTTCACCAGTGACAAAGCCG  >  CP009273/3649315‑3649603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`