BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,667,959	C→A	100%	intergenic (+224/‑187)	yhjD → / → yhjE	inner membrane putative BrbK family alternate lipid exporter/MFS superfamily sugar transport 1 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,667,959	0	C	A	99.2%	781.0 / ‑1.9	242	intergenic (+224/‑187)	yhjD/yhjE	inner membrane putative BrbK family alternate lipid exporter/MFS superfamily sugar transport 1 family protein
Reads supporting (aligned to +/- strand): ref base C (1/1); new base A (106/134); total (107/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 242 total aligned reads displayed.

CGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAACCTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTGCTG  >  CP009273/3667815‑3668102

 gcCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGa                                                                                                                                             <  2:1024792/148‑1 (MQ=255)

  ccAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGaa                                                                                                                                            <  1:990391/148‑1 (MQ=255)

  ccAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGaa                                                                                                                                            <  2:1583886/148‑1 (MQ=255)

      catcgcatcCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCa                                                                                                                                        >  1:3025608/1‑148 (MQ=255)

       atcgcatcCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAg                                                                                                                                       <  1:790423/148‑1 (MQ=255)

       atcgcatcCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAg                                                                                                                                       <  2:1331615/148‑1 (MQ=255)

        tcgcatcCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGt                                                                                                                                      <  1:1381480/148‑1 (MQ=255)

         cgcatcCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGtt                                                                                                                                     <  2:677731/148‑1 (MQ=255)

         cgcatcCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGtt                                                                                                                                     <  1:2405215/148‑1 (MQ=255)

           catcCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGtttt                                                                                                                                   <  2:633484/148‑1 (MQ=255)

             tcCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAACTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTAt                                                                                                                                 <  2:2739089/148‑1 (MQ=255)

              ccGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATc                                                                                                                                <  2:1057880/148‑1 (MQ=255)

              ccGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATc                                                                                                                                >  2:2328163/1‑148 (MQ=255)

                ggCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCa                                                                                                                              >  1:3380936/1‑148 (MQ=255)

                 gCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCAc                                                                                                                             <  2:3271150/148‑1 (MQ=255)

                 gCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCAc                                                                                                                             <  1:739522/148‑1 (MQ=255)

                  cTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACt                                                                                                                            >  1:985640/1‑148 (MQ=255)

                    ataATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTAt                                                                                                                          >  1:1761102/1‑148 (MQ=255)

                    ataATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTAt                                                                                                                          <  1:855458/148‑1 (MQ=255)

                      aaTGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTAttt                                                                                                                        >  2:1415205/1‑148 (MQ=255)

                      aaTGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTAttt                                                                                                                        >  1:1075989/1‑148 (MQ=255)

                      aaTGCGCACACAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCCGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTAttt                                                                                                                        <  2:3414359/148‑1 (MQ=255)

                                tAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTAt                                                                                                              >  2:2351175/1‑148 (MQ=255)

                                tAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTAt                                                                                                              >  2:3092382/1‑148 (MQ=255)

                                  aCCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTAtgt                                                                                                            <  1:479386/148‑1 (MQ=255)

                                   ccTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTAtgtg                                                                                                           >  1:702492/1‑148 (MQ=255)

                                    ctctTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGa                                                                                                          <  2:208218/148‑1 (MQ=255)

                                        tGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCa                                                                                                      <  2:442916/148‑1 (MQ=255)

                                        tGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCa                                                                                                      <  1:2907291/148‑1 (MQ=255)

                                        tGAAACACGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTGAGACCCCCCCCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAACCCAGTTTTTTCCACTATTTATATAATTATGTGAAGCa                                                                                                      <  2:2016846/148‑1 (MQ=255)

                                            aCTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGACACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTc                                                                                                  >  1:774549/1‑148 (MQ=255)

                                            aCTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTc                                                                                                  <  1:1493005/148‑1 (MQ=255)

                                            aCTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCAGTCCAACTCATAACCCCAGCATATACATCCAGGTGGTAACTTTTCTTTAAACTGAAACCAGTTTTATCCACTATTGATAAAATTATGTGAAGCATTTc                                                                                                  <  1:1449521/148‑1 (MQ=255)

                                              tCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCCGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATACACTATTTATAAAATTATGTGAAGCATTTCAt                                                                                                >  2:2424840/1‑148 (MQ=255)

                                              tCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCAt                                                                                                >  1:517262/1‑148 (MQ=255)

                                               cGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATa                                                                                               <  1:3468024/148‑1 (MQ=255)

                                                 tCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATaga                                                                                             >  2:474496/1‑148 (MQ=255)

                                                  ccccAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATagaa                                                                                            >  1:1411648/1‑148 (MQ=255)

                                                   cccAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATagaag                                                                                           >  1:383116/1‑148 (MQ=255)

                                                     cAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAgaagaa                                                                                         >  1:3059656/1‑148 (MQ=255)

                                                       gagCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCCTAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGaaaa                                                                                       <  2:2803123/148‑1 (MQ=255)

                                                        agCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGaaaaa                                                                                      <  2:1193544/148‑1 (MQ=255)

                                                         gCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAAt                                                                                     >  2:2372401/1‑148 (MQ=255)

                                                           ctctTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCa                                                                                   <  1:542368/148‑1 (MQ=255)

                                                           ctctTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCa                                                                                   <  2:2972401/148‑1 (MQ=255)

                                                              ttCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTg                                                                                <  2:1659734/148‑1 (MQ=255)

                                                               tCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTgg                                                                               >  2:3223570/1‑148 (MQ=255)

                                                                   ccATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTa                                                                           >  2:253807/1‑148 (MQ=255)

                                                                      tCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAAc                                                                        <  2:530438/148‑1 (MQ=255)

                                                                        tATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACAt                                                                      <  1:2244914/148‑1 (MQ=255)

                                                                          tttGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTa                                                                    <  2:956591/148‑1 (MQ=255)

                                                                           ttGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTAt                                                                   <  2:3286116/148‑1 (MQ=255)

                                                                           ttGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTAt                                                                   <  1:3286391/148‑1 (MQ=255)

                                                                              ggAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATccc                                                                >  1:2269647/1‑148 (MQ=255)

                                                                              ggAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATccc                                                                >  1:502453/1‑148 (MQ=255)

                                                                              ggAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATccc                                                                <  1:3344421/148‑1 (MQ=255)

                                                                              ggAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATccc                                                                <  1:2499339/148‑1 (MQ=255)

                                                                               gAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGCAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATcccc                                                               >  1:2561941/1‑148 (MQ=255)

                                                                                  cAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCttt                                                            >  1:2732010/1‑148 (MQ=255)

                                                                                  cAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCttt                                                            <  1:1300561/148‑1 (MQ=255)

                                                                                  cAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCttt                                                            <  2:2848713/148‑1 (MQ=255)

                                                                                  cAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCttt                                                            <  1:3389345/148‑1 (MQ=255)

                                                                                   aaaCAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCtttt                                                           >  1:1735904/1‑148 (MQ=255)

                                                                                    aaCAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCttttt                                                          >  1:1006540/1‑148 (MQ=255)

                                                                                      cAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGc                                                        >  1:3203614/1‑148 (MQ=255)

                                                                                       aaTTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGcc                                                       <  2:442445/148‑1 (MQ=255)

                                                                                       aaTTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATACCCTTTTTgac                                                       >  2:1846253/1‑146 (MQ=255)

                                                                                         tttCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTg                                                     >  1:2386147/1‑148 (MQ=255)

                                                                                           tCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCGCTGGCCTAAACATTATCCCCTTTTTGCCTGAt                                                   <  2:3025608/148‑1 (MQ=255)

                                                                                              ttCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGAtttt                                                <  2:2176799/148‑1 (MQ=255)

                                                                                              ttCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGAtttt                                                >  2:2152526/1‑148 (MQ=255)

                                                                                                  aaCTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGAc                                            >  1:1101867/1‑148 (MQ=255)

                                                                                                   aCTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGAcc                                           >  1:2392329/1‑148 (MQ=255)

                                                                                                   aCTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGAcc                                           >  1:3105796/1‑148 (MQ=255)

                                                                                                    cTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCa                                          >  2:2707613/1‑148 (MQ=255)

                                                                                                          aCCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCcg                                    >  2:1341892/1‑148 (MQ=255)

                                                                                                          aCCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCcg                                    <  1:2851774/148‑1 (MQ=255)

                                                                                                           ccccAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCcgc                                   >  1:427925/1‑148 (MQ=255)

                                                                                                            cccAGCATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCcgcg                                  <  2:1541926/148‑1 (MQ=255)

                                                                                                                 cATATAAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTg                             >  1:1338398/1‑148 (MQ=255)

                                                                                                                    ataAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTa                          >  2:951646/1‑148 (MQ=255)

                                                                                                                     taAATCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTac                         >  2:2048755/1‑148 (MQ=255)

                                                                                                                       aaTCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTacac                       <  2:1872693/148‑1 (MQ=255)

                                                                                                                       aaTCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTacac                       >  2:738938/1‑148 (MQ=255)

                                                                                                                        aTCCAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTAcaca                      <  1:150882/148‑1 (MQ=255)

                                                                                                                          ccAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACAtt                    >  2:2714606/1‑148 (MQ=255)

                                                                                                                           cAGTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTg                   <  2:3139669/148‑1 (MQ=255)

                                                                                                                             gTTGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGaa                 <  2:796995/148‑1 (MQ=255)

                                                                                                                              ttGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGaaa                >  1:2627637/1‑148 (MQ=255)

                                                                                                                              ttGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGaaa                >  1:2046015/1‑148 (MQ=255)

                                                                                                                              ttGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGaaa                <  1:307362/148‑1 (MQ=255)

                                                                                                                               tGGTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAat               >  2:2659762/1‑148 (MQ=255)

                                                                                                                                ggTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGGCCATTTCCGCGATTTGTTACACATTGAAata              >  1:2757999/1‑148 (MQ=255)

                                                                                                                                 gTAACTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAatat             <  1:2111754/148‑1 (MQ=255)

                                                                                                                                   aaCTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCa           <  2:985640/148‑1 (MQ=255)

                                                                                                                                    aCTTTTATTTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCAc          >  1:1688471/1‑148 (MQ=255)

                                                                                                                                     cTTTTAATTAAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACt         <  2:1994/148‑1 (MQ=255)

                                                                                                                                         tatttaAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTg     <  2:3481369/148‑1 (MQ=255)

                                                                                                                                         tatttaAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTg     <  2:1744300/148‑1 (MQ=255)

                                                                                                                                            ttaAACTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTGCtg  <  2:3423918/148‑1 (MQ=255)

CGCCAGCATCGCATCCGGCTATAATGCGCACATAACCTCTTGAAACTCGTCCCCAGAGCCTCTTCAGCCATCTATTTGGGAGCAAACAATTTCATTCCAACTCATAACCCCAGCATATAAATCCAGTTGGTAACTTTTATTTAACCTGAAACCAGTTTTATCCACTATTTATAAAATTATGTGAAGCATTTCATAGAAGAAAAATCACTGGCCTAAACATTATCCCCTTTTTGCCTGATTTTTGACCATTTCCGCGATTTGTTACACATTGAAATATCACTTTTGCTG  >  CP009273/3667815‑3668102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`