Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,727,580 C→A 100% A167A (GCC→GCA)  xylH → D‑xylose ABC transporter permease subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,727,5800CA99.5% 625.6 / ‑4.3 194A167A (GCC→GCA) xylHD‑xylose ABC transporter permease subunit
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (103/90);  total (103/91)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 198 total aligned reads displayed.

TGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACC  >  CP009273/3727435‑3727724
                                                                                                                                                 |                                                                                                                                                
tGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGcgcagc                                                                                                                                                <  1:2053081/148‑1 (MQ=255)
 gggACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACcagcgcagcg                                                                                                                                               <  1:413183/148‑1 (MQ=255)
 gggACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACcagcgcagcg                                                                                                                                               <  1:2538456/148‑1 (MQ=255)
    aCTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATg                                                                                                                                            <  1:1116577/148‑1 (MQ=255)
     cTGCTTCTTGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGt                                                                                                                                           >  1:2553547/1‑148 (MQ=255)
       gCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTca                                                                                                                                         >  2:2408115/1‑148 (MQ=255)
             cGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAAtt                                                                                                                                   <  1:493838/148‑1 (MQ=255)
              ggTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTg                                                                                                                                  >  2:326951/1‑148 (MQ=255)
               gTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTgg                                                                                                                                 <  2:3419722/148‑1 (MQ=255)
                  ccTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGcc                                                                                                                              >  1:1518209/1‑147 (MQ=255)
                      gAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCCTGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                          >  1:2358518/1‑148 (MQ=255)
                      gAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTg                                                                                                                                  >  1:1719097/1‑140 (MQ=255)
                      gAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                          >  1:3214895/1‑148 (MQ=255)
                      gAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                          >  1:3056342/1‑148 (MQ=255)
                      gAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGc                                                                                                                          <  2:37152/148‑1 (MQ=255)
                       aaCGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCt                                                                                                                         >  2:1647662/1‑148 (MQ=255)
                        aCGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTa                                                                                                                        >  2:116888/1‑148 (MQ=255)
                        aCGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTa                                                                                                                        <  2:1741302/148‑1 (MQ=255)
                            aTGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTAtctc                                                                                                                    <  2:260308/148‑1 (MQ=255)
                                    tCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAg                                                                                                            <  2:3094997/148‑1 (MQ=255)
                                     cgcgTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGt                                                                                                           >  2:3479873/1‑148 (MQ=255)
                                      gcgTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTa                                                                                                          <  1:1510662/148‑1 (MQ=255)
                                         tACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCg                                                                                                       <  1:2689045/148‑1 (MQ=255)
                                              tAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtc                                                                                                  >  1:1614916/1‑148 (MQ=255)
                                              tAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtc                                                                                                  >  1:486105/1‑148 (MQ=255)
                                               aaaGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAAAGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTtca                                                                                                 >  1:119086/1‑148 (MQ=255)
                                                    cccTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCAtt                                                                                            <  1:2637698/148‑1 (MQ=255)
                                                     ccTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTg                                                                                           >  1:3436018/1‑148 (MQ=255)
                                                      cTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTgg                                                                                          >  1:818036/1‑148 (MQ=255)
                                                        tCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGgcg                                                                                        >  1:1401541/1‑148 (MQ=255)
                                                        tCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGgcg                                                                                        <  2:1726495/148‑1 (MQ=255)
                                                            ttATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCt                                                                                    >  2:1513763/1‑148 (MQ=255)
                                                            ttATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCt                                                                                    >  2:782744/1‑148 (MQ=255)
                                                             tATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCtt                                                                                   <  2:3206617/148‑1 (MQ=255)
                                                             tATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCtt                                                                                   <  2:3205152/148‑1 (MQ=255)
                                                              aTTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTg                                                                                  <  1:2519026/148‑1 (MQ=255)
                                                              aTTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAAGTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTg                                                                                  >  2:409827/1‑148 (MQ=255)
                                                               ttGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTgg                                                                                 <  1:3331172/148‑1 (MQ=255)
                                                               ttGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATAACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTgg                                                                                 >  2:102022/1‑148 (MQ=255)
                                                                  tCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCtt                                                                              >  1:1244332/1‑148 (MQ=255)
                                                                     cccTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAAt                                                                           <  1:794414/148‑1 (MQ=255)
                                                                     cccTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAAt                                                                           <  1:794448/148‑1 (MQ=255)
                                                                     cccTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAAt                                                                           >  2:1151288/1‑148 (MQ=255)
                                                                       cTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATgg                                                                         >  1:3150131/1‑148 (MQ=255)
                                                                        tCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGc                                                                        >  1:307973/1‑148 (MQ=255)
                                                                        tCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGAACCGGCTTCATCATTGGCGCGCTTGTCTTAATGGc                                                                        >  1:309401/1‑148 (MQ=255)
                                                                         cgcgGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCt                                                                       <  2:2935090/148‑1 (MQ=255)
                                                                         cgcgGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCt                                                                       <  2:2182009/148‑1 (MQ=255)
                                                                          gcgGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCtt                                                                      <  2:1542918/148‑1 (MQ=255)
                                                                            gggCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCtttt                                                                    <  2:2582074/148‑1 (MQ=255)
                                                                            gggCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCtttt                                                                    <  1:109735/148‑1 (MQ=255)
                                                                            gggCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCtttt                                                                    <  1:1884560/148‑1 (MQ=255)
                                                                             ggCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTttg                                                                   >  2:447664/1‑148 (MQ=255)
                                                                                 tGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttgg                                                               <  2:1118972/148‑1 (MQ=255)
                                                                                 tGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttgg                                                               <  1:700109/148‑1 (MQ=255)
                                                                                 tGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttgg                                                               >  1:1730523/1‑148 (MQ=255)
                                                                                  gTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttggt                                                              >  2:863157/1‑148 (MQ=255)
                                                                                   ttGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTgttggtt                                                             <  1:2774426/148‑1 (MQ=255)
                                                                                      gCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGc                                                          <  1:1304493/148‑1 (MQ=255)
                                                                                         tttCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAAt                                                       <  2:2767943/148‑1 (MQ=255)
                                                                                          ttCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATg                                                      >  2:1865239/1‑148 (MQ=255)
                                                                                               ggCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGcgcg                                                 <  2:2291662/148‑1 (MQ=255)
                                                                                                 cATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGga                                               >  2:806648/1‑148 (MQ=255)
                                                                                                 cATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGga                                               <  2:1689844/148‑1 (MQ=255)
                                                                                                   tACTCATTGGCGTCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGgaag                                             >  1:1295623/1‑148 (MQ=255)
                                                                                                    aCTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGgaaga                                            >  1:2275070/1‑148 (MQ=255)
                                                                                                    aCTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGgaaga                                            <  1:2421425/148‑1 (MQ=255)
                                                                                                      tCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAAt                                          >  2:2468988/1‑148 (MQ=255)
                                                                                                       cATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATg                                         <  1:64562/148‑1 (MQ=255)
                                                                                                         ttGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATgcg                                       <  2:2553547/148‑1 (MQ=255)
                                                                                                            gCATCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCg                                    <  2:2753063/148‑1 (MQ=255)
                                                                                                              aTCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTc                                  <  2:3286670/148‑1 (MQ=255)
                                                                                                              aTCACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCAACATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTc                                  <  2:945570/148‑1 (MQ=255)
                                                                                                                cACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAg                                >  1:817380/1‑148 (MQ=255)
                                                                                                                cACCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAg                                >  2:3179737/1‑148 (MQ=255)
                                                                                                                 aCCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAgg                               >  1:2828761/1‑148 (MQ=255)
                                                                                                                 aCCAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAgg                               >  2:1541877/1‑148 (MQ=255)
                                                                                                                  cccaCGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGc                              <  2:2021931/145‑1 (MQ=255)
                                                                                                                   cAACGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCt                             >  1:1999271/1‑148 (MQ=255)
                                                                                                                    aaCGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCtt                            <  1:2324170/148‑1 (MQ=255)
                                                                                                                    aaCGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCtt                            <  1:561093/148‑1 (MQ=255)
                                                                                                                     aCGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCttt                           <  2:1327896/148‑1 (MQ=255)
                                                                                                                     aCGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCttt                           >  2:1389122/1‑148 (MQ=255)
                                                                                                                      cGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTg                          <  2:488721/148‑1 (MQ=255)
                                                                                                                      cGGCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTg                          >  2:2916539/1‑148 (MQ=255)
                                                                                                                       ggCACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTgg                         <  1:102022/148‑1 (MQ=255)
                                                                                                                         cACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGt                       >  2:3070497/1‑148 (MQ=255)
                                                                                                                         cACGACTGTATCCCCCACCAGCGCAGCGATGTCACAAATTCGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCAACATTGGCGCGCTTGGCTTAATGGCTTTGGTTGGGTGGCAATGGCGCGGAAGAATCCGCCGTCACGCTTTGGGt                       >  1:2153448/1‑148 (MQ=255)
                                                                                                                             aCTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTAc                   <  1:863157/148‑1 (MQ=255)
                                                                                                                              cTGTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCAGTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCGTTGGGTTTACa                  >  2:1193439/1‑148 (MQ=255)
                                                                                                                              cTGAATCCCCCACCAGCGCAGCGATGTCACAAATAGGGCAAATCTATCACCCCGCCAGTACCGGCTTAATCATTGGCGCGCTTGGAGTAATGTCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGACGTCAGGCGTTGGGTTTACa                  >  2:2637837/1‑148 (MQ=255)
                                                                                                                               tGTATCCCCCACCAGCGCAGCGATGTCACACATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAg                 <  2:2358518/148‑1 (MQ=255)
                                                                                                                                gTATCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGt                <  2:601644/148‑1 (MQ=255)
                                                                                                                                  aTCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGtct              <  1:2428049/148‑1 (MQ=255)
                                                                                                                                   tCCCCCACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGtctc             >  1:825782/1‑148 (MQ=255)
                                                                                                                                    cccccACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTcc            >  2:194729/1‑148 (MQ=255)
                                                                                                                                      cccACCAGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCgg          >  2:2448051/1‑148 (MQ=255)
                                                                                                                                        caccaGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGcc        <  1:731867/148‑1 (MQ=255)
                                                                                                                                            aGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTa    <  2:1740662/148‑1 (MQ=255)
                                                                                                                                            aGCGCAGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTa    <  1:3479873/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TGGGACTGCTTCTCGGTGCCTGGAACGGATGGTGGGTCGCGTACCGTAAAGTCCCTTCATTTATTGTCACCCTCGCGGGCATGTTGGCATTTCGCGGCATACTCATTGGCATCACCAACGGCACGACTGTATCCCCCACCAGCGCCGCGATGTCACAAATTGGGCAAAGCTATCTCCCCGCCAGTACCGGCTTCATCATTGGCGCGCTTGGCTTAATGGCTTTTGTTGGTTGGCAATGGCGCGGAAGAATGCGCCGTCAGGCTTTGGGTTTACAGTCTCCGGCCTCTACC  >  CP009273/3727435‑3727724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑