Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,767,410 G→T 100% V590V (GTG→GTT)  mtlA → fused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,767,4100GT98.9% 578.3 / ‑1.8 179V590V (GTG→GTT) mtlAfused mannitol‑specific PTS enzymes: IIA components/IIB components/IIC components
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (86/91);  total (88/91)
Fisher's exact test for biased strand distribution p-value = 2.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 179 total aligned reads displayed.

CCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTA  >  CP009273/3767265‑3767553
                                                                                                                                                 |                                                                                                                                               
ccccGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTAt                                                                                                                                               >  1:1057966/1‑148 (MQ=255)
 cccGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTAtt                                                                                                                                              >  1:495160/1‑148 (MQ=255)
 cccGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTAtt                                                                                                                                              >  2:370575/1‑148 (MQ=255)
 cccGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTAtt                                                                                                                                              <  1:2642453/148‑1 (MQ=255)
 cccGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTAtt                                                                                                                                              <  1:1713944/148‑1 (MQ=255)
   cGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTgg                                                                                                                                            >  1:2011650/1‑148 (MQ=255)
   cGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTgg                                                                                                                                            >  1:2994610/1‑148 (MQ=255)
    gACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGt                                                                                                                                           >  1:802007/1‑148 (MQ=255)
     aCTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTa                                                                                                                                          <  1:894034/148‑1 (MQ=255)
      cTTTTCTGGGTGAGTCTATCGCGGTGCCTCACGGTTCGGTTGAAGCGAAAGATCGCGTACTGAATTCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTATGTGAAGAAGAAGTTGTCATTGCCCGTCTGGTTATTGGTAt                                                                                                                                         <  1:863899/148‑1 (MQ=255)
      cTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGTTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTAt                                                                                                                                         >  2:579538/1‑148 (MQ=255)
      cTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTAt                                                                                                                                         >  1:377507/1‑148 (MQ=255)
           cTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATtgctg                                                                                                                                    >  2:2653238/1‑148 (MQ=255)
             gggTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGcc                                                                                                                                  <  1:729838/148‑1 (MQ=255)
              ggTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGccc                                                                                                                                 >  2:1973274/1‑148 (MQ=255)
               gTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCg                                                                                                                                >  2:3026571/1‑148 (MQ=255)
                tGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGt                                                                                                                               >  1:2692333/1‑148 (MQ=255)
                tGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGt                                                                                                                               >  2:157841/1‑148 (MQ=255)
                 gAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTa                                                                                                                              >  1:767015/1‑148 (MQ=255)
                  aGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaa                                                                                                                             <  2:2337720/148‑1 (MQ=255)
                     cTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTaacaa                                                                                                                          <  1:1371919/148‑1 (MQ=255)
                        tCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGa                                                                                                                       >  2:2805943/1‑148 (MQ=255)
                        tCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGa                                                                                                                       >  2:2538802/1‑148 (MQ=255)
                            ggTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGcac                                                                                                                   <  1:1227072/148‑1 (MQ=255)
                              tGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACAt                                                                                                                 >  1:3336055/1‑148 (MQ=255)
                                 cacaCGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCa                                                                                                              <  1:1351557/148‑1 (MQ=255)
                                   cacGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAgg                                                                                                            >  2:2949406/1‑148 (MQ=255)
                                     cggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGATGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTGACAACGAGCACATTCAGGtt                                                                                                          <  1:235625/148‑1 (MQ=255)
                                      ggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTa                                                                                                         <  1:58012/148‑1 (MQ=255)
                                      ggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTa                                                                                                         <  2:2222130/148‑1 (MQ=255)
                                      ggtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTa                                                                                                         <  1:2978450/148‑1 (MQ=255)
                                       gtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTAt                                                                                                        <  2:2692333/148‑1 (MQ=255)
                                       gtacggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTAt                                                                                                        <  2:2134/148‑1 (MQ=255)
                                           ggtTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCAcc                                                                                                    <  2:672220/148‑1 (MQ=255)
                                              tGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGNAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGc                                                                                                 <  1:1780024/148‑1 (MQ=255)
                                              tGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGc                                                                                                 <  1:1024738/148‑1 (MQ=255)
                                              tGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGc                                                                                                 <  1:567120/148‑1 (MQ=255)
                                               gAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGcc                                                                                                <  1:3382712/148‑1 (MQ=255)
                                                 aGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTg                                                                                              >  1:1431101/1‑148 (MQ=255)
                                                  gCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGa                                                                                             >  2:1720954/1‑148 (MQ=255)
                                                  gCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGa                                                                                             >  2:1103142/1‑148 (MQ=255)
                                                  gCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGa                                                                                             <  2:754400/148‑1 (MQ=255)
                                                     aaaGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCa                                                                                          >  1:1520651/1‑148 (MQ=255)
                                                      aaGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCaa                                                                                         >  2:189799/1‑148 (MQ=255)
                                                      aaGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCaa                                                                                         >  2:2386351/1‑148 (MQ=255)
                                                        gATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATg                                                                                       <  1:1331798/148‑1 (MQ=255)
                                                        gATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGGACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATg                                                                                       <  2:3234149/148‑1 (MQ=255)
                                                          tCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                     <  1:2301885/148‑1 (MQ=255)
                                                          tCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCa                                                                                     >  2:2368220/1‑148 (MQ=255)
                                                           cgcgTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCAc                                                                                    <  2:1787064/148‑1 (MQ=255)
                                                             cgTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTg                                                                                  >  1:3294407/1‑148 (MQ=255)
                                                               tACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGga                                                                                <  2:1997877/148‑1 (MQ=255)
                                                                   gAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGgatgat                                                                            <  1:3253506/148‑1 (MQ=255)
                                                                      aaCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAg                                                                         >  1:3140225/1‑148 (MQ=255)
                                                                       aCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGt                                                                        <  2:2105841/148‑1 (MQ=255)
                                                                       aCGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGAGGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGt                                                                        <  2:2105685/148‑1 (MQ=255)
                                                                          ggCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCg                                                                     >  1:188619/1‑148 (MQ=255)
                                                                            cgtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTc                                                                   <  1:3179001/148‑1 (MQ=255)
                                                                             gtcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCa                                                                  <  2:2779113/148‑1 (MQ=255)
                                                                              tcgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCAt                                                                 >  2:1964089/1‑148 (MQ=255)
                                                                               cgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                >  1:986910/1‑148 (MQ=255)
                                                                               cgtGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATc                                                                >  1:2177585/1‑148 (MQ=255)
                                                                                 tgtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGa                                                              <  1:1989442/148‑1 (MQ=255)
                                                                                  gtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAg                                                             >  1:875739/1‑148 (MQ=255)
                                                                                  gtTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAg                                                             >  1:1270175/1‑148 (MQ=255)
                                                                                               ccGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGcacaca                                                >  2:2539771/1‑148 (MQ=255)
                                                                                               ccGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGcacaca                                                >  1:1898947/1‑148 (MQ=255)
                                                                                                  gAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACAcacca                                             >  1:2192406/1‑148 (MQ=255)
                                                                                                         tGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGt                                      >  2:1418632/1‑148 (MQ=255)
                                                                                                         tGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGt                                      >  2:3364376/1‑148 (MQ=255)
                                                                                                          gcgcTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTg                                     >  2:2872855/1‑148 (MQ=255)
                                                                                                             cTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGAt                                  >  2:2453337/1‑148 (MQ=255)
                                                                                                               tCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGa                                <  1:3318367/148‑1 (MQ=255)
                                                                                                               tCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAAGGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGa                                <  2:296647/148‑1 (MQ=255)
                                                                                                                  gTGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGt                             <  1:3063076/148‑1 (MQ=255)
                                                                                                                   tGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTg                            <  2:495160/148‑1 (MQ=255)
                                                                                                                   tGAAGAAGAAGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTg                            >  2:2646042/1‑148 (MQ=255)
                                                                                                                    gaagaagaaGATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGc                           <  2:1206500/148‑1 (MQ=255)
                                                                                                                    gaagaagaaGATGACAGTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGc                           <  2:1987504/148‑1 (MQ=255)
                                                                                                                     aagaagaagATGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCt                          <  1:2831512/148‑1 (MQ=255)
                                                                                                                       gaagaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTgg                        >  2:265974/1‑148 (MQ=255)
                                                                                                                          gaagaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAAc                     >  2:1432025/1‑148 (MQ=255)
                                                                                                                          gaagaTGACATTGCCCGTCTGGTTAGTGGTATTGCTGCCCGTAACAACGAGCCCATAAAGGTAATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACAACACCAGCGTGGATGAAGTGCTGGAAc                     >  2:1431259/1‑148 (MQ=255)
                                                                                                                             gaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgc                  <  1:159465/148‑1 (MQ=255)
                                                                                                                             gaTGACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgc                  <  1:159495/148‑1 (MQ=255)
                                                                                                                              aTGACCTTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCACTGCACTGGAGGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgct                 <  2:320189/148‑1 (MQ=255)
                                                                                                                              aTGACATTGCCCGTCTGGTTATTGGTATTTCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAActgct                 <  2:1707021/148‑1 (MQ=255)
                                                                                                                                gACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTgg               >  2:779466/1‑148 (MQ=255)
                                                                                                                                gACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTgg               <  2:2011650/148‑1 (MQ=255)
                                                                                                                                gACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTgg               >  2:1772048/1‑148 (MQ=255)
                                                                                                                                gACATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTgg               >  2:1771870/1‑148 (MQ=255)
                                                                                                                                  cATTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCa             <  2:3345063/148‑1 (MQ=255)
                                                                                                                                   aTTGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAg            <  1:285317/148‑1 (MQ=255)
                                                                                                                                     tGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt          <  2:986910/148‑1 (MQ=255)
                                                                                                                                     tGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt          <  1:1973274/148‑1 (MQ=255)
                                                                                                                                     tGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt          <  1:2653238/148‑1 (MQ=255)
                                                                                                                                     tGCCCGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGt          <  2:2177585/148‑1 (MQ=255)
                                                                                                                                        ccGTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgt       <  2:2338856/148‑1 (MQ=255)
                                                                                                                                          gTCTGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGACATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaa     >  2:700550/1‑148 (MQ=255)
                                                                                                                                             tGGTTATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCgtaagta  >  1:2215602/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCCCGACTTATCTGGGTGAGTCTATCGCGGTGCCACACGGTACGGTTGAAGCGAAAGATCGCGTACTGAAAACGGGCGTCGTGTTCTGCCAGTACCCGGAAGGCGTGCGCTTCGGTGAAGAAGAAGATGACATTGCCCGTCTGGTGATTGGTATTGCTGCCCGTAACAACGAGCACATTCAGGTTATCACCAGCCTGACCAATGCACTGGATGATGAGTCCGTCATCGAGCGTCTGGCACACACCACCAGCGTGGATGAAGTGCTGGAACTGCTGGCAGGTCGTAAGTA  >  CP009273/3767265‑3767553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑