Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,786,682 C→A 9.8% C121F (TGT→TTT)  yibB ← YibB family protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,786,6820CA 9.8% 570.0 / 8.5 216C121F (TGT→TTT) yibBYibB family protein, function unknown
Reads supporting (aligned to +/- strand):  ref base C (89/98);  new base A (7/22);  total (96/120)
Fisher's exact test for biased strand distribution p-value = 2.59e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 221 total aligned reads displayed.

TTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTATATCGATAACGATAACCG  >  CP009273/3786535‑3786829
                                                                                                                                                   |                                                                                                                                                   
 tACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAACCCCAAGTTGATAGCTTTATTTAAAAAATAGTCCATAAGGTTACa                                                                                                                                                    >  1:2466895/1‑148 (MQ=255)
  aCTTTCATCAATTGTGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACAt                                                                                                                                                   <  2:2276083/148‑1 (MQ=255)
       catcaAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCaa                                                                                                                                              <  2:1843524/148‑1 (MQ=255)
         tcaAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCaaaa                                                                                                                                            <  1:295568/148‑1 (MQ=255)
          caAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTGTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAAc                                                                                                                                           >  1:3045639/1‑148 (MQ=255)
            aaTGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAATGTTACATACCAAAACAt                                                                                                                                         >  1:1074664/1‑148 (MQ=255)
               gggAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATAct                                                                                                                                      <  1:87715/148‑1 (MQ=255)
               gggAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATAct                                                                                                                                      >  2:3223988/1‑148 (MQ=255)
                ggAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATActc                                                                                                                                     >  1:548748/1‑148 (MQ=255)
                 gAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATActct                                                                                                                                    >  1:141561/1‑148 (MQ=255)
                   aaTCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTgg                                                                                                                                  <  2:708095/148‑1 (MQ=255)
                    aTCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGt                                                                                                                                 <  2:2044122/148‑1 (MQ=255)
                    aTCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGt                                                                                                                                 <  1:2594331/148‑1 (MQ=255)
                     tCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTGAATAAGCCCAAGTTGATAGCTTTATTTAAAAAATAGGCCTTAATGTTAAAAACCAAAACATACTCTGGTg                                                                                                                                >  1:1510170/1‑148 (MQ=255)
                     tCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTAAAAAATATGCCTTAATGTTACATACCAAAACATAATCTGGTg                                                                                                                                >  1:1221596/1‑148 (MQ=255)
                     tCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTAAAAAAAATGCCTTAATGTTACATAACAAAAAAAACTCTGGTg                                                                                                                                >  1:1510191/1‑148 (MQ=255)
                     tCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCAAGTTGATAGCTTTAATTAAAAAATATGCCTTAAGGTTAAATAACAAAACAAAATATGGTg                                                                                                                                >  1:1011581/1‑148 (MQ=255)
                      cccAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATAATCTGGTGa                                                                                                                               >  2:2845178/1‑148 (MQ=255)
                      cccAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTAACTAAGCCCATGTTGATAGCTTTATTTAAAAAATAGGCCTTAAGGTTACATAACAAAACATACTCTGGTGa                                                                                                                               >  1:71829/1‑148 (MQ=255)
                          aaTTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAg                                                                                                                           >  2:491083/1‑148 (MQ=255)
                          aaTTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAg                                                                                                                           >  2:2689824/1‑148 (MQ=255)
                            ttttCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAATCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTa                                                                                                                         >  1:2745651/1‑148 (MQ=255)
                             tttCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCCGTAc                                                                                                                        >  1:2803450/1‑148 (MQ=255)
                                cAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCAAGTTTATAGCTTTATTTACAAAATAGGCCTTAAGGTTAAATACCAAAACAAACTCTGGTGACCAGTAatct                                                                                                                     >  1:987890/1‑148 (MQ=255)
                                   tCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAATCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTAAATAACAAAAAATACTCTGGTGACCAGTACTCTggg                                                                                                                  >  1:2087776/1‑148 (MQ=255)
                                    ccTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGt                                                                                                                 <  2:1767021/148‑1 (MQ=255)
                                     cTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGtt                                                                                                                <  1:2397268/148‑1 (MQ=255)
                                     cTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGtt                                                                                                                <  1:829680/148‑1 (MQ=255)
                                     cTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGtt                                                                                                                <  1:3384994/148‑1 (MQ=255)
                                      tCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGttt                                                                                                               <  2:1519945/148‑1 (MQ=255)
                                          gTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTGACTAAGCCCATGTTTATAGCTTTATTTACAAAATATGCCTTAAGGTTACATACCAAAACATACTCGGGTGTCCAGTACTCTGGGGTTTTTa                                                                                                           >  1:1864144/1‑148 (MQ=255)
                                           ttACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTaa                                                                                                          <  2:2453608/148‑1 (MQ=255)
                                           ttACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTaa                                                                                                          >  2:3259560/1‑148 (MQ=255)
                                           ttACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTaa                                                                                                          <  2:1551725/148‑1 (MQ=255)
                                             aCATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAAtt                                                                                                        <  1:2807478/148‑1 (MQ=255)
                                              cATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTg                                                                                                       <  2:184448/148‑1 (MQ=255)
                                               aTTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGt                                                                                                      <  1:3203477/148‑1 (MQ=255)
                                               aTTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGt                                                                                                      >  1:837753/1‑148 (MQ=255)
                                                tttGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTTTTAGCTTTTTTTAAAAAATAGGCCTTAAGGTTAAATACCAAAAAATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTTTc                                                                                                     >  1:966746/1‑148 (MQ=255)
                                                    gCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATTTTTATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACAAAAAAATACACAGGTGACCAGGAATCTGGGTTTTTTAAATGTCGAgg                                                                                                 >  1:3440935/1‑148 (MQ=255)
                                                      ttATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCt                                                                                               >  2:2178708/1‑148 (MQ=255)
                                                               tAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTAt                                                                                      <  1:2178708/148‑1 (MQ=255)
                                                                  ccAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTg                                                                                   <  2:352629/148‑1 (MQ=255)
                                                                     aaaTCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTaa                                                                                <  1:434342/148‑1 (MQ=255)
                                                                     aaaTCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTaa                                                                                >  2:2232283/1‑148 (MQ=255)
                                                                     aaaTCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTaa                                                                                <  2:1451956/148‑1 (MQ=255)
                                                                     aaaTCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCGTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTaa                                                                                >  2:2232614/1‑148 (MQ=255)
                                                                      aaTCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTaaa                                                                               <  2:2394009/148‑1 (MQ=255)
                                                                      aaTCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCGTTATTTAAAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTTATTGTCGAGGCTCAAGTCTATTTGTaaa                                                                               >  1:421028/1‑148 (MQ=255)
                                                                      aaTCTATCCACGCAACAAGAGGCGTGTTCACTAAGCCCAGGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAAAAAACTATGGTGACCAGTACTATGGGTTTTTTAATTTTCGAGGCTCAAGTCTATTTTTaaa                                                                               >  1:1273388/1‑148 (MQ=255)
                                                                        tCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACGCTGGGTTTTTTTATTGTCGAGGCTCAAGTCTATTTTTaaaag                                                                             >  1:3130840/1‑146 (MQ=255)
                                                                             ccACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTAAAAAATAGGCCGTAAGGTTACATACCAAAACATAAACTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGGCTATTTGTAAACGACTCa                                                                        >  1:515251/1‑148 (MQ=255)
                                                                             ccACGAAACAAGTGGCGTTTACACTAAGCCCTTGTTTATAGGTTTATTTACAAATTAGGCATTATGGTTAAATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTATTTGTCGTGGCTCATGTCTATTTGTAAACGACTCa                                                                        <  2:2423496/148‑1 (MQ=255)
                                                                                cgaaacaaTCGGCGTTTTAACTAAGCCCATGTTGATAGATTTATTTACAAAATTGGCCTTAAGGTTAAATACCAAAACATACTCTGGTGACCCGTACTCGGGTTTTTTTATTTGTCGCGGCTCAAGTCTATTTGTAAACGACTCATCt                                                                     <  2:1384970/145‑1 (MQ=255)
                                                                                cgaaacaaGAGGCGTTTTAACTAAGCCCATTTTTATAGCTTTATTAAAAAAATAGGCCTTATGGTTACATACCAAAACATACTCTGGTGACCAGTACTGGGGTTTTTTTATTTTTCGCGGATCAAGTCTATTTGTACACGACTCATCt                                                                     <  2:1361980/145‑1 (MQ=255)
                                                                                  caacaaGAGGCGTTTTCACTAACCCCATTTTGATAGCTTTATTTACAAAATAGGCCATAACGGTACATACCAAAACAAAAACTGGTGACCAGTACTCTTGGTTTTTTAATTGTCGAGGCTCAAGTCTAATTTTAAACAAATCATCttt                                                                   >  2:2070167/1‑148 (MQ=255)
                                                                                     caaGAGGCTTTTTCAATAAGCCCATTTTTATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGTTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTg                                                                <  2:248680/148‑1 (MQ=255)
                                                                                       agagGCGTTTTCACTACGCCCATGTTGATAGCTTTATTTAAAAAATAGGCCTTAAGGTTACATACAAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGaa                                                              <  2:3148115/148‑1 (MQ=255)
                                                                                            gttttaCACTAAGCCCTTTTTTATAGCTTTATTAAAAAAATAGGCCTTAAGGTAACAAACAAAAACATACTCTGGTGACCTGTACTCTGGTTTTTTTATTTGTCGCGGCTCATGTATATTTGTAAACGACTCATCTTTTTGAAttttt                                                         <  1:1438775/146‑1 (MQ=255)
                                                                                            cGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAAttttt                                                         >  1:936660/1‑148 (MQ=255)
                                                                                             gTTTTCACTAAGCCAATGTTGATAGCTTTTTTTACAAAATAGGCCTTAAGGTTACATACCAAAAAATACTGTGTTGACCAGTACTCTGGTTTTTTTAATTGTCGAGTCTAAAGTCTATTTGTAAACGACTCATCTTTTTGAAtttttt                                                        <  2:1995675/148‑1 (MQ=255)
                                                                                               tttCAATAAGCCCTTGTTGATATCTTTATTTACAAATTTGGCATAAAGGTTACAAACAAAAAAATACTGTGGTCACCAGTACTCGGGTTTTTTTATGTGTCGCGGCTCAAGTCTTTTTGAAAACGACTCATCTTTTTGAATTTTTTCg                                                      <  2:2090486/148‑1 (MQ=255)
                                                                                                 tCACTAAGCCCTGTTTGATCGGTTTATTTAAAAAATTGGAGTTAAGTTTAAATACCAAAACTTATTCTGGTTACCATTAATCTTTGTTTTTTATTTGTCGAGGATAAAGTGTATTTTTAAACGACTCATCTTTTTGAATTTTTTCGAt                                                    <  2:575923/148‑1 (MQ=255)
                                                                                                  caatAAGCCCATGTTTATTGCTTTATTAAAAAAATCGGCCTTACGGTAACATACAAAAAAATACGCTGGTGACCAGTACTGTGGTTTTTTTAATTGTCGCGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATa                                                   <  2:1492297/145‑1 (MQ=255)
                                                                                                     tAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGa                                                >  2:2014613/1‑148 (MQ=255)
                                                                                                      aaGCCCTTGTTTATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACGCGGGTGACCAGTACTCTGGTTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGAc                                               <  2:3034931/148‑1 (MQ=255)
                                                                                                         cccATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTc                                            >  1:2478732/1‑148 (MQ=255)
                                                                                                          ccATGTTTTTTGATTTATTTAAAAAATAGGCATTACGTTAAAAAACCAAAAAATACTCTGGTGACAAGTACTCTGGTTTTTTTAATTGTCGCGGCTCAATTCTATTTTAAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCt                                           <  1:2230125/148‑1 (MQ=255)
                                                                                                           cATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTg                                          >  2:2625571/1‑148 (MQ=255)
                                                                                                           cATGTTGATAGCTTTATTTACAAAATAGGCCATAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTg                                          >  2:367537/1‑148 (MQ=255)
                                                                                                            aTGTTTATAGCTTTATTTAAAAAATAGGCATTAAGGTTACAAACCAAAACATACTCTGGTGACCAGTACTCGGGTTTTTTTAATTGTCTAGGCTCAAGTATTTTTTTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGa                                         <  2:1066373/148‑1 (MQ=255)
                                                                                                             tGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCTTTACTCTGGTTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGat                                        <  2:3460339/148‑1 (MQ=255)
                                                                                                              ttttatcgCTTTATTTAAAAAATTGGCCTTAAGGTTACATACAAAAACATACTCTGGTGACCAGTACTCTGTTTTTTTTATTTGTCGAGGCTCACGTCTATTTGAAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGata                                       <  2:1565281/141‑1 (MQ=255)
                                                                                                              gTTTATAGCTTTATTTACAAAATAGGCCTCAAGGTTACATACCAAAACATACGCTGGTGCCCAGTACTGTGGGTTTTTTAATTGTCGAGGCTCAAGTATATTTTAAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGata                                       <  2:3304602/148‑1 (MQ=255)
                                                                                                              gTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGata                                       >  1:3302253/1‑148 (MQ=255)
                                                                                                                tGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGatata                                     >  2:1192533/1‑148 (MQ=255)
                                                                                                                tGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGatata                                     >  2:2909246/1‑148 (MQ=255)
                                                                                                                  aTAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATc                                   >  1:2309063/1‑148 (MQ=255)
                                                                                                                    atatttatttaAAAAATAGGCCTTAAGTTTAAATACCAAAAAATACTGTGGTCACCTGTACTTTGTTTTTTTTATTTGTCGCGGCTCAAGTATATTTTAAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTa                                 <  1:2241203/145‑1 (MQ=255)
                                                                                                                     gCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTaa                                >  1:2610454/1‑148 (MQ=255)
                                                                                                                      cTTTATTTAAAAATTAGGCATTATGGTTACATCCAAAAACATACTCGGGTTACCAGTACTGTGGTTTTTTTAATTGTCGAGGCTCATGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTaaa                               <  1:1450230/148‑1 (MQ=255)
                                                                                                                         tatttcCAAAATAGGCCTTAAGTTTACATACCAAAAAATAGTGTGGTGACCATTACTGTGGTTTTTTTATTTGTCGAGGATCACGTCTTTTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAAttt                            <  2:935421/148‑1 (MQ=255)
                                                                                                                         tattaaAAAAATAGGCATTATGTTTAAAAACAAAAACATACTCTGGTCACCATTACTCTGGTTTTTTTATTGGTCGCGGCTCAAGTATATTTTTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAAttt                            <  1:3379796/148‑1 (MQ=255)
                                                                                                                           tttaCAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCt                          >  2:3173111/1‑148 (MQ=255)
                                                                                                                              aaaaaaTAGGCCTTAAGGTTACAAACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTATTTGTCGAGGCTAAAGTCTATTTGAAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCtttt                       <  2:1011581/146‑1 (MQ=255)
                                                                                                                               aaaaaTAGCCCTTACGGTAACAAAAAAAAACAACCTGTGGTGACCTGTAAGGTGGTTTTTTTATTGGTCGCGGATCAAGTCTTTTTTAAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCttttt                      <  1:2426985/147‑1 (MQ=255)
                                                                                                                                aaaaTAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCtttttt                     >  2:1376537/1‑148 (MQ=255)
                                                                                                                                 aaaTAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTta                    >  2:2883575/1‑148 (MQ=255)
                                                                                                                                 aaaTAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTta                    >  1:429905/1‑148 (MQ=255)
                                                                                                                                   aTAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTtata                  >  1:3268975/1‑148 (MQ=255)
                                                                                                                                   aTAGGCATTAAGGTTACATACAAAAACAAACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTTTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTtata                  <  2:3373530/148‑1 (MQ=255)
                                                                                                                                    tAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTatat                 >  1:628911/1‑148 (MQ=255)
                                                                                                                                     aGGCCTTATGTTTAAATAAAAAAAAATCCTGTGGTTACCATTAGTCTGGTTTTTTTATTTGTCGCGGCACAAGTTTTTTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTATATc                <  2:305479/148‑1 (MQ=255)
                                                                                                                                      ggCATTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGAAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTATATcg               <  2:1155383/148‑1 (MQ=255)
                                                                                                                                       gCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTATATcga              >  2:2121769/1‑148 (MQ=255)
                                                                                                                                       gCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTATATcga              >  2:1600172/1‑148 (MQ=255)
                                                                                                                                             aGGTTACATACCAAAACATCCTCTGGTTACCAGTAATCTGGGTTTTTTATTTGTGTAGGATCAAGTATATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTATATcgataacga        <  1:2845178/148‑1 (MQ=255)
                                                                                                                                             aGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTATATcgataacga        >  1:2584806/1‑148 (MQ=255)
                                                                                                                                              ggTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTATATcgataacgat       >  1:1617926/1‑148 (MQ=255)
                                                                                                                                                ttACATACCAAAACATACTCTGGTGACCTGTACTCTGGTTTTTTTAATTGTCGAGGCACACTTCTTTTTTTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTATATcgataacgataa     <  2:2945532/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTACTTTCATCAAATGGGAAATCCCAAATTTTCAATCCTCGAGTTACATTTGGCTTATGGCAATAGCCAAAATCTATCCACGCAACAAGAGGCGTTTTCACTAAGCCCATGTTGATAGCTTTATTTACAAAATAGGCCTTAAGGTTACATACCAAAACATACTCTGGTGACCAGTACTCTGGGTTTTTTAATTGTCGAGGCTCAAGTCTATTTGTAAACGACTCATCTTTTTGAATTTTTTCGATACGACTTCTGATATATCTAAATTTCTTTTTTATATCGATAACGATAACCG  >  CP009273/3786535‑3786829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑