Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,822,339 A→C 10.7% Q12P (CAA→CCA) ‡ xanP → xanthine permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,822,3390AC10.7% 359.8 / 4.8 193Q12P (CAA→CCA) ‡xanPxanthine permease
Reads supporting (aligned to +/- strand):  ref base A (88/78);  new base C (21/6);  total (109/84)
Fisher's exact test for biased strand distribution p-value = 2.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGC  >  CP009273/3822192‑3822485
                                                                                                                                                   |                                                                                                                                                  
 ataAAAAAGTGCATTTATCTCATTTAAATATTTATATGATGATGTGTCAAACGTTTTCTTTTCTCTTTCGGTAAGCTAAAATGCCCGCTATGCTACAACGGGATGTTTTTCGATGTCTGTTTCCACCCACGAGTCAGAAAATGAGCac                                                                                                                                                   >  2:3149936/1‑147 (MQ=255)
 ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                   <  1:3247676/148‑1 (MQ=255)
 ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                   <  1:2183539/148‑1 (MQ=255)
 ataAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCaa                                                                                                                                                   >  1:2135462/1‑148 (MQ=255)
    aaaaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCg                                                                                                                                                >  2:2636407/1‑148 (MQ=255)
     aaaaGTGCATTGATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCgg                                                                                                                                               <  1:630751/148‑1 (MQ=255)
      aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                              >  2:962038/1‑148 (MQ=255)
      aaaGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGt                                                                                                                                              <  2:1008194/148‑1 (MQ=255)
        aGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCACCCGTTg                                                                                                                                            >  1:301989/1‑148 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTgcg                                                                                                                                          >  1:2562696/1‑148 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                          <  2:2684271/148‑1 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                          >  2:342144/1‑148 (MQ=255)
          tGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTgcg                                                                                                                                          >  1:249483/1‑148 (MQ=255)
             aTTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAg                                                                                                                                       >  1:3465739/1‑148 (MQ=255)
              tttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGa                                                                                                                                      >  2:3394121/1‑148 (MQ=255)
               ttATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGAc                                                                                                                                     >  1:964083/1‑148 (MQ=255)
                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCa                                                                                                                                  >  1:1460500/1‑148 (MQ=255)
                  tctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCCGTGCGcacacaca                                                                                                                                  >  1:2788692/1‑148 (MQ=255)
                   ctcATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTTCGCAGACTaaa                                                                                                                                 >  1:762331/1‑148 (MQ=255)
                     cATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGGAACCCGTTGCGCAGACACaaaa                                                                                                                               >  1:916046/1‑148 (MQ=255)
                      aTTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAc                                                                                                                              <  2:2138045/148‑1 (MQ=255)
                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                             >  2:195936/1‑148 (MQ=255)
                       tttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACa                                                                                                                             <  2:1243490/148‑1 (MQ=255)
                        ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCTCAGACTCAAAACat                                                                                                                            >  2:1459266/1‑147 (MQ=255)
                        ttAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAg                                                                                                                            >  2:132123/1‑148 (MQ=255)
                            atatTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGAGCAACCGGTTGCGCAGACAAAAAAAATCGaa                                                                                                                        >  2:1562063/1‑148 (MQ=255)
                            atatTTAGATGACTAAGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATCCCCTCTATGCTACCACCGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCGGATGAGCAGACACAAAACACCGaa                                                                                                                        >  2:1830834/1‑148 (MQ=255)
                              atTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAACCGAAca                                                                                                                      >  1:1866956/1‑147 (MQ=255)
                              atTTAGATGAGGATGTGGCAAAAGTTTGCTTTTATCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGATTCCACCCACGAGTCAGAAAATGCGCCACCCGTAGCGCAGACAAAAAAAACCGAAca                                                                                                                      >  1:484539/1‑147 (MQ=255)
                               tttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCCGACTCAAAAAAGCGAAcag                                                                                                                     >  1:2198335/1‑146 (MQ=255)
                                ttAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGCGCAGACTCAAAAAAGCGAACTGa                                                                                                                    >  1:2848803/1‑148 (MQ=255)
                                 tAGATGAGGATGTGCAACACTTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGCTTTGCTACCACGTGATTTTTTTCGATGTCTGTTTCCCCCCTCTAGTAAGAAATTGCGCAACGGGTTGCCCAGACTCAAAACAGCGAACTGAt                                                                                                                   <  1:323732/148‑1 (MQ=255)
                                 tAGAGGTGGATGTGGCAACCGTTTGCTTTTCTCTTGCGGACAGCTAAAATGCCCGCTTTGCTACCACGGGTTTTTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACGGGTTGCGCAGACTCAAAACAGCGAACTGAt                                                                                                                   <  2:2312423/148‑1 (MQ=255)
                                   gaggAGGTTGTGCCAACCGTTTGCTTTTCTCTTTCGGTCAGATAAAATGCCCGCTTTGCTACCACGGTTTTTTTTTCTTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGAttt                                                                                                                 <  1:504847/145‑1 (MQ=255)
                                       aGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGGAACCCGTTGCGCAGACTCAAAAAAGCCAACAGATTTAACg                                                                                                             >  1:1929396/1‑148 (MQ=255)
                                        tcaTGTGGCAAACGATTGCTTTTCTCTTTCGCTCAGCTAAAATGCCCGCTTTGCTACCACCGGATTGATTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGAGCAACCCGTTGCGCATAATCAAAACATCGAACAGATTTAcctt                                                                                                            >  2:2886313/3‑146 (MQ=255)
                                        ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACACCGAACTGATTTACCGt                                                                                                            >  2:1137293/1‑148 (MQ=255)
                                        ggATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTAAAAACAGCGAACTGATTTACCGt                                                                                                            >  2:3068188/1‑148 (MQ=255)
                                         gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCGGTTGCGCCGACTCCAAACAGCGAACGGATTTACCGTc                                                                                                           >  1:3421880/1‑148 (MQ=255)
                                         gATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTc                                                                                                           >  2:3059955/1‑148 (MQ=255)
                                          aTGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCt                                                                                                          <  2:2856177/148‑1 (MQ=255)
                                           tgtgGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCCCCCGTTTCGCAGACTCAAAACAACGAACAGATTTAACCTCtt                                                                                                         >  1:1304411/1‑148 (MQ=255)
                                               gCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACACTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAg                                                                                                     <  2:2835185/148‑1 (MQ=255)
                                                 aaaCGTTTGCTTTTCTCTTTCGGTGAGCTATATTGCCGGGTTTGCTACCACGGGATTGTTTTCGTTGTCTGTTTCCACCCACGAGTCAGAAAATGCGCAACGGGGTGCGCAGACTCAAATCAGCGAACTGTTTTACCGTCTTGAAGAt                                                                                                   <  2:1043180/148‑1 (MQ=255)
                                                 aaaCGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCACACTCAAAACAGCCAACAGAATTACCCTCTTGAAGAt                                                                                                   >  2:1234805/1‑148 (MQ=255)
                                                    cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAAGGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                <  2:2654024/148‑1 (MQ=255)
                                                    cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGt                                                                                                >  2:2728632/1‑148 (MQ=255)
                                                    cGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCCGGTTCGCCGACTCAAAAAAGCGAACAGATTTAACGTCTTGAAGATCGt                                                                                                >  1:2534373/1‑148 (MQ=255)
                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGTTTTACCGTCTTGAAGATCGTccg                                                                                             >  2:2011465/1‑148 (MQ=255)
                                                       ttGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTacg                                                                                             >  2:615046/1‑148 (MQ=255)
                                                        tGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCGCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTccgc                                                                                            <  2:873489/148‑1 (MQ=255)
                                                           tcttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGAATCAAAAAAGCGAAATGATTTAACGTCTTGAAGATCGTCcgccgc                                                                                         >  2:3288570/3‑148 (MQ=255)
                                                             ttCTCTTTGGGTCAGAAAAAATGCCCTCTTTGCTACCACGGTTTTTTTTTCGTTGTCTGTTCCCACCCTCGATACAGAAATTGCGCAACGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                       <  1:3169201/148‑1 (MQ=255)
                                                             ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCCACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCtt                                                                                       >  2:1404045/1‑148 (MQ=255)
                                                             ttCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGCAGATCGTCcgccgcgt                                                                                       >  2:3233212/1‑146 (MQ=255)
                                                                       gTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAAccc                                                                             >  2:2504813/1‑148 (MQ=255)
                                                                          aGCTAAAATGCCCGCTTTGCTACCACGGGTTTGTTTTCGATTTCTGTTACCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGt                                                                          <  2:3299211/148‑1 (MQ=255)
                                                                            cTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAAAAGCGAACTGATTTTCCGTCTTGTATATCGTCCGCCGCTTCCTCAAACCCTGttt                                                                        >  1:2642691/1‑148 (MQ=255)
                                                                                aaTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCGGTTTgccg                                                                    >  2:2106884/1‑148 (MQ=255)
                                                                                 aTGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTgccgc                                                                   <  2:1001348/148‑1 (MQ=255)
                                                                                     ccGCTTTGCTACCACGGGATTGTTTTCGATGGCTGTTTCCACCCTCGAGTCAGAAAATGCGCACCCCGTTGCGCAGACTCAAAAAAGCGAACTGATTTACCGGCTTGAAGATGGTCCGCCGCGTCCTCAAACCCAGGGTGGCGCCTGt                                                               >  1:2419223/1‑148 (MQ=255)
                                                                                       gCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCa                                                             >  2:2724232/1‑148 (MQ=255)
                                                                                            gCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATc                                                        <  2:1731903/148‑1 (MQ=255)
                                                                                               cccACGGGTTTGTTTTCGATGTGTGTCCCCACCCTCTTTACACAAATTGCCCCCCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                     <  1:795502/147‑1 (MQ=255)
                                                                                               aCCACGGGATTGTTTTCGTTGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                     <  2:419966/148‑1 (MQ=255)
                                                                                               aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCCGTTGGGCAGACTCAAAAAAGCGAACAGATTTAACGTCTTTAATATAGTCCGCCGCGTCCTCAAAACCAGTTTTCCGCCTGTCAGCAActgc                                                     >  1:1627500/1‑148 (MQ=255)
                                                                                               aCCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGACAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATctgc                                                     <  2:471099/148‑1 (MQ=255)
                                                                                                ccACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGGTGCGCAGACTCAAAAAAGCGAACTGATTTAACGTCTTTAATATCGTCCGCCGCGTCCTCAAAACCAGTTTGCCGCCAGTCAGCATctgct                                                    >  1:2216603/1‑148 (MQ=255)
                                                                                                  aCGGGATTGTTTTCGATGTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                  <  2:521461/148‑1 (MQ=255)
                                                                                                  aCGGGATTGATTACGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTgg                                                  <  2:2285812/148‑1 (MQ=255)
                                                                                                    gggATTGTTTTCGTTGTGTGTTCCCACCCTCTAGTCAGAAAATGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCg                                                <  1:2382507/148‑1 (MQ=255)
                                                                                                     ggATTGTTTTCGATGTCTGTTTCCACCCGCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGa                                               <  2:1398559/148‑1 (MQ=255)
                                                                                                      gATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCACACTCAAAAAACCGAACAGATTTACCGTATTGAATATACTCCGCCCCTACCTCAAAACCAGTTTGCCGCCGGTCAGCAGCTGCTGGCGAt                                              >  2:454146/1‑148 (MQ=255)
                                                                                                        ttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                            >  2:2462937/1‑148 (MQ=255)
                                                                                                        ttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGt                                            >  2:568623/1‑148 (MQ=255)
                                                                                                        ttGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCCCAACCGGATGCGCAGACTCAAAAAACCGAAAAGATTTAACGTAATGAATATAGTCCGCCGCCTCCTCAAACCCTGTATGGCGCCACTCAGCATCAGCTGGCGATGt                                            >  2:1787774/1‑148 (MQ=255)
                                                                                                          tttttGGTTGTCTGTTCCCACCCTCTATTCAGAATTTGCCCAGCGGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTc                                          <  1:1313056/147‑1 (MQ=255)
                                                                                                           ttttCGTTGTCTTTTACCACCCTCTAGTCAGAAAATGCGCAACCGGTGCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                         <  1:3457824/148‑1 (MQ=255)
                                                                                                           ttttCGTTGTCTGTTACCACCCTCGAGACAGAAAATGCGCACCGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                         <  1:585876/148‑1 (MQ=255)
                                                                                                           ttttCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCg                                         <  2:1412820/148‑1 (MQ=255)
                                                                                                            tttCGATGTCTGTTTCCACCCTCGAGTCCGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGt                                        <  2:1653722/148‑1 (MQ=255)
                                                                                                                  tGTCTGTTTCCACCCTCGAGTCAGAAAATGGGCCCCCCGTTGCGCAGACTCAAAACACCCCACAGATTTACCGTCTTGAAGATAGTCCGCCGCTTCCTCAAACCCGGTTTGGCGCCAGTCAGCATCTGCTGGCGATGTTCGTTGGGGt                                  >  1:1031678/1‑148 (MQ=255)
                                                                                                                  tGTCTGTTTCCACCCTCGAGTCACAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGt                                  <  2:301989/148‑1 (MQ=255)
                                                                                                                   gTCTTTATCCACCCGCGAGTCACAAACTGCGCCACGGGGTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                 <  1:2605828/148‑1 (MQ=255)
                                                                                                                   gTCTGTTTCCCCCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTg                                 <  2:1541408/148‑1 (MQ=255)
                                                                                                                     cTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTTCGCAGACTCAAAACACCGAACTGATTTAACGTCTTTAAGATAGTCCGCCCCGTCCACAAAACCAGTTTTCCGCCTGTCACCAAATGCTGGCGATTTTCGTTGCGGTGAt                               >  1:1967391/1‑148 (MQ=255)
                                                                                                                         ttCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTAACGTCTTGAAGATCGTCCGCCGCTTCCTCCAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTAGTTTCGGGGATCACg                           >  2:3314466/1‑148 (MQ=255)
                                                                                                                                  cGAGTCATAAAAGGCGCAACCGGTTGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTa                  <  1:391550/148‑1 (MQ=255)
                                                                                                                                    tgccataaatTGCTCACCCGGTTCCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTAtt                <  1:230883/142‑1 (MQ=255)
                                                                                                                                    aGTCAGAAAATGCGCAACCGGTTGCGCACACTCAAAACAGCGAACTGATTTACCGTCTTGAACATCGTCCGCCGCGTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCCGCGCTAtt                >  2:3288861/1‑148 (MQ=255)
                                                                                                                                      tCAGAAACGGCGCACCGGGTGGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa              <  1:3327451/148‑1 (MQ=255)
                                                                                                                                      tCAGAAAATGCGCAACCGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa              <  2:1605824/148‑1 (MQ=255)
                                                                                                                                      tCACAAAAGGCGCACCGGGTCGCCCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTaa              <  1:1540800/148‑1 (MQ=255)
                                                                                                                                            aaTGCGCACCCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGcc        <  2:2081380/148‑1 (MQ=255)
                                                                                                                                             cggcgcAAGGGGTGGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCa       <  1:1598885/146‑1 (MQ=255)
                                                                                                                                               gcgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg     <  2:2534373/148‑1 (MQ=255)
                                                                                                                                               gcgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg     >  2:2388593/1‑148 (MQ=255)
                                                                                                                                               gcgcAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAgg     <  2:2931936/148‑1 (MQ=255)
                                                                                                                                                  cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc  >  1:2279097/1‑148 (MQ=255)
                                                                                                                                                  cAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGgcgc  >  1:2278787/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TATAAAAAAGTGCATTTATCTCATTTAAATATTTAGATGAGGATGTGGCAAACGTTTGCTTTTCTCTTTCGGTCAGCTAAAATGCCCGCTTTGCTACCACGGGATTGTTTTCGATGTCTGTTTCCACCCTCGAGTCAGAAAATGCGCAACCGGTTGCGCAGACTCAAAACAGCGAACTGATTTACCGTCTTGAAGATCGTCCGCCGCTTCCTCAAACCCTGTTTGCCGCCTGTCAGCATCTGCTGGCGATGTTCGTTGCGGTGATCACGCCAGCGCTATTAATCTGCCAGGCGC  >  CP009273/3822192‑3822485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑