Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,828,206 T→A 100% S362C (AGT→TGT)  yicJ ← putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,828,2060TA98.5% 674.4 / ‑0.3 204S362C (AGT→TGT) yicJputative transporter
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base A (94/107);  total (96/108)
Fisher's exact test for biased strand distribution p-value = 6.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 204 total aligned reads displayed.

GTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGG  >  CP009273/3828061‑3828350
                                                                                                                                                 |                                                                                                                                                
gTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGa                                                                                                                                                <  1:1111388/148‑1 (MQ=255)
   aaTAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATcc                                                                                                                                             <  2:2263505/148‑1 (MQ=255)
      aGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCgg                                                                                                                                          <  1:869208/148‑1 (MQ=255)
       gcgcAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGt                                                                                                                                         <  2:1162969/148‑1 (MQ=255)
       gcgcAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGt                                                                                                                                         <  1:1197885/148‑1 (MQ=255)
         gcAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCa                                                                                                                                       <  2:1883724/148‑1 (MQ=255)
          cAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAg                                                                                                                                      >  1:514670/1‑148 (MQ=255)
          cAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAg                                                                                                                                      >  1:2281060/1‑148 (MQ=255)
              aatGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGt                                                                                                                                  >  1:2708616/1‑148 (MQ=255)
               atgatgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGtt                                                                                                                                 <  2:2812182/148‑1 (MQ=255)
                 gatgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTa                                                                                                                               <  1:1873064/148‑1 (MQ=255)
                  atgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTAc                                                                                                                              <  1:1466426/148‑1 (MQ=255)
                  atgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTAc                                                                                                                              <  1:1565808/148‑1 (MQ=255)
                   tgCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTAcc                                                                                                                             <  1:3134621/148‑1 (MQ=255)
                    gcgaaTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCa                                                                                                                            <  2:1435545/145‑1 (MQ=255)
                       aaTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTg                                                                                                                         >  1:1020468/1‑148 (MQ=255)
                        aTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGc                                                                                                                        >  1:1246511/1‑148 (MQ=255)
                        aTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGc                                                                                                                        >  2:2697709/1‑148 (MQ=255)
                        aTCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGc                                                                                                                        >  1:2101125/1‑148 (MQ=255)
                         tCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGca                                                                                                                       >  2:2362799/1‑148 (MQ=255)
                         tCGTGGCGCTGTTCTGCGCTTTTTCTGCCGAATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCACGCTCAGGCGTTTACCATTGca                                                                                                                       >  2:599989/1‑148 (MQ=255)
                          cGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcac                                                                                                                      >  2:2402209/1‑148 (MQ=255)
                            tGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcacca                                                                                                                    >  2:2909513/1‑148 (MQ=255)
                            tGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcacca                                                                                                                    <  1:2187753/148‑1 (MQ=255)
                            tGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAACATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGcacca                                                                                                                    >  2:2021294/1‑148 (MQ=255)
                              gcgcTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACt                                                                                                                  <  1:748293/148‑1 (MQ=255)
                                gcTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCg                                                                                                                <  2:279256/148‑1 (MQ=255)
                                 cTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGc                                                                                                               <  2:3487017/148‑1 (MQ=255)
                                 cTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGc                                                                                                               <  2:1746939/148‑1 (MQ=255)
                                 cTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGc                                                                                                               <  1:348018/148‑1 (MQ=255)
                                    ttCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGt                                                                                                            <  2:299476/148‑1 (MQ=255)
                                      cTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAg                                                                                                          <  2:1544025/148‑1 (MQ=255)
                                      cTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAg                                                                                                          <  2:2116782/148‑1 (MQ=255)
                                        gcgcTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTc                                                                                                        >  1:1930889/1‑148 (MQ=255)
                                          gcTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGa                                                                                                      <  1:2582757/148‑1 (MQ=255)
                                           cTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGAc                                                                                                     >  1:1979513/1‑148 (MQ=255)
                                            tttttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACg                                                                                                    <  1:964954/148‑1 (MQ=255)
                                             ttttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACgg                                                                                                   >  2:3249293/1‑148 (MQ=255)
                                             ttttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACgg                                                                                                   >  2:3249099/1‑148 (MQ=255)
                                              tttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGt                                                                                                  <  1:2909513/148‑1 (MQ=255)
                                               ttCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTa                                                                                                 <  2:2313540/148‑1 (MQ=255)
                                                  tGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCg                                                                                              <  2:657309/148‑1 (MQ=255)
                                                     cGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAc                                                                                           <  1:2989252/148‑1 (MQ=255)
                                                      gCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAca                                                                                          >  1:1970099/1‑148 (MQ=255)
                                                          catATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatcat                                                                                      <  2:1801313/148‑1 (MQ=255)
                                                          catATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatcat                                                                                      <  1:2988244/148‑1 (MQ=255)
                                                          catATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGAcatcat                                                                                      >  1:2064707/1‑148 (MQ=255)
                                                                   cATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTg                                                                             >  1:1641731/1‑148 (MQ=255)
                                                                    aTAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTgg                                                                            <  2:72639/148‑1 (MQ=255)
                                                                     tAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGa                                                                           >  2:1574269/1‑148 (MQ=255)
                                                                      aaGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGAt                                                                          >  2:3390731/1‑148 (MQ=255)
                                                                       aGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATa                                                                         >  1:1845967/1‑148 (MQ=255)
                                                                        gCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAg                                                                        >  2:3205108/1‑148 (MQ=255)
                                                                        gCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAg                                                                        >  2:1460627/1‑148 (MQ=255)
                                                                              aTCCAGCCGATAAGAGCGCCGCCG‑AGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCAc                                                                 <  2:3223406/148‑1 (MQ=255)
                                                                               tCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCAc                                                                 >  1:2375349/1‑148 (MQ=255)
                                                                                ccAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCAcc                                                                <  1:2339891/148‑1 (MQ=255)
                                                                                    ccGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTCCCCACTGGATAGGTGTCACCAGtt                                                            >  2:380201/1‑148 (MQ=255)
                                                                                      gATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGa                                                          >  1:1518129/1‑148 (MQ=255)
                                                                                          agagCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCa                                                      >  2:1234377/1‑148 (MQ=255)
                                                                                              cgccgcCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacac                                                  >  2:145342/1‑148 (MQ=255)
                                                                                               gccgccGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacaca                                                 <  2:2100845/148‑1 (MQ=255)
                                                                                                ccgccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacacac                                                >  1:398025/1‑148 (MQ=255)
                                                                                                ccgccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacacac                                                <  1:2813659/148‑1 (MQ=255)
                                                                                                ccgccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacacac                                                <  1:1253969/148‑1 (MQ=255)
                                                                                                ccgccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAacacac                                                >  2:610646/1‑148 (MQ=255)
                                                                                                  gccgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCa                                              <  2:2810969/148‑1 (MQ=255)
                                                                                                    cgAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAAt                                            <  1:3438648/148‑1 (MQ=255)
                                                                                                      aaGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCa                                          <  2:430822/148‑1 (MQ=255)
                                                                                                       aGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCAc                                         >  1:3260636/1‑148 (MQ=255)
                                                                                                        ggCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACg                                        <  1:2772565/148‑1 (MQ=255)
                                                                                                          ccAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGaa                                      <  1:2946869/148‑1 (MQ=255)
                                                                                                          ccAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGaa                                      <  1:916962/148‑1 (MQ=255)
                                                                                                           cAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAg                                     >  2:1013464/1‑148 (MQ=255)
                                                                                                             aCCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGAt                                   >  1:2417312/1‑148 (MQ=255)
                                                                                                              ccccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATg                                  <  1:1359052/148‑1 (MQ=255)
                                                                                                              ccccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATg                                  <  1:1127322/148‑1 (MQ=255)
                                                                                                              ccccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATg                                  >  2:1016606/1‑148 (MQ=255)
                                                                                                               cccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGa                                 <  1:2786496/148‑1 (MQ=255)
                                                                                                               cccAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGa                                 <  1:1189437/148‑1 (MQ=255)
                                                                                                                 cAGTTTGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAg                               <  1:2865160/148‑1 (MQ=255)
                                                                                                                     ttGAGCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACaa                           >  1:3170889/1‑148 (MQ=255)
                                                                                                                         gCACAAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACAt                       >  2:2889124/1‑148 (MQ=255)
                                                                                                                          cacaAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATa                      <  2:2708390/148‑1 (MQ=255)
                                                                                                                          cacaAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATa                      <  1:1810808/148‑1 (MQ=255)
                                                                                                                          cacaAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATa                      >  1:1334417/1‑148 (MQ=255)
                                                                                                                          cacaAACAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATa                      <  2:1389850/148‑1 (MQ=255)
                                                                                                                              aaCAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGa                  >  1:2872906/1‑148 (MQ=255)
                                                                                                                              aaCAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGa                  >  1:962391/1‑148 (MQ=255)
                                                                                                                              aaCAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGa                  <  1:2697709/148‑1 (MQ=255)
                                                                                                                                cAGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATg                >  1:2219190/1‑148 (MQ=255)
                                                                                                                                 aGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGc               <  2:459795/148‑1 (MQ=255)
                                                                                                                                 aGCGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACAGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGc               >  1:734866/1‑148 (MQ=255)
                                                                                                                                   cGTGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTg             >  2:522857/1‑148 (MQ=255)
                                                                                                                                     tGCCAGCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGc           <  1:3078543/148‑1 (MQ=255)
                                                                                                                                          gCAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCa      >  1:2883984/1‑148 (MQ=255)
                                                                                                                                           cAAAACAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCAt     >  1:70246/1‑148 (MQ=255)
                                                                                                                                            aaaaCTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATg    >  1:3327613/1‑148 (MQ=255)
                                                                                                                                            aaaaCAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATg    <  2:1970099/148‑1 (MQ=255)
                                                                                                                                              aaCAGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATggg  >  1:1732874/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GTGAATAGCGCAATAATGATGCTAATCGTGGCGCTGTTCTGCGCTTTTTCTGCCGCATCATATCCGCCATAAGCCAGCATCCAGCCGATAAGAGCGCCGCCGAAGGCCAACCCCAGTTTGAGCACAAACAGCGTGCCAGCAAAACTGATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGG  >  CP009273/3828061‑3828350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑