Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,830,819 T→G 100% F169L (TTT→TTG)  setC → putative arabinose efflux transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,830,8190TG100.0% 804.8 / NA 197F169L (TTT→TTG) setCputative arabinose efflux transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (102/95);  total (102/95)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 197 total aligned reads displayed.

CAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCG  >  CP009273/3830674‑3830962
                                                                                                                                                 |                                                                                                                                               
cAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAg                                                                                                                                               <  1:641619/148‑1 (MQ=255)
   aCCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGttt                                                                                                                                            <  1:2636869/148‑1 (MQ=255)
      cGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTaa                                                                                                                                         >  2:1730406/1‑148 (MQ=255)
         aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGt                                                                                                                                      <  1:1159026/148‑1 (MQ=255)
         aaaTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGt                                                                                                                                      <  1:3464795/148‑1 (MQ=255)
           aTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGa                                                                                                                                    >  1:2792028/1‑148 (MQ=255)
           aTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGa                                                                                                                                    >  2:2997944/1‑148 (MQ=255)
           aTGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGa                                                                                                                                    <  1:2398128/148‑1 (MQ=255)
            tGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGAt                                                                                                                                   >  2:1891789/1‑148 (MQ=255)
            tGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGAt                                                                                                                                   >  1:3130524/1‑148 (MQ=255)
            tGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGAt                                                                                                                                   <  1:156126/148‑1 (MQ=255)
             gTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATg                                                                                                                                  >  1:52674/1‑148 (MQ=255)
             gTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATg                                                                                                                                  >  1:2741575/1‑148 (MQ=255)
             gTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATg                                                                                                                                  >  1:2429114/1‑148 (MQ=255)
             gTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATg                                                                                                                                  >  2:3434080/1‑148 (MQ=255)
               tcgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCACTGGGATTGAGTTTTAAAGTGATGTa                                                                                                                                >  1:1032815/1‑148 (MQ=255)
                cgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAt                                                                                                                               <  2:2177888/148‑1 (MQ=255)
                cgccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAt                                                                                                                               <  1:1262733/148‑1 (MQ=255)
                  ccctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGGTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAtct                                                                                                                             <  2:2732801/148‑1 (MQ=255)
                   cctcgcccGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTAtctc                                                                                                                            >  2:576249/1‑148 (MQ=255)
                          cGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTg                                                                                                                     >  1:756694/1‑148 (MQ=255)
                          cGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTg                                                                                                                     <  2:3055032/148‑1 (MQ=255)
                              aaCACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCAt                                                                                                                 >  1:3472454/1‑148 (MQ=255)
                               acacGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCCATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATc                                                                                                                >  1:2199770/1‑148 (MQ=255)
                                cacGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTTCTCAGATCTCGCTTGCCTGGGTTATCGGGCAACCGCTCGCTTATGAACTGGCAATGGGATGGAGTTTTAAAGTGATGTATCTGAACGCTGCCATCg                                                                                                               >  2:3305628/1‑148 (MQ=255)
                                   gCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAt                                                                                                            >  1:934856/1‑148 (MQ=255)
                                         aGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgttg                                                                                                      >  2:2470888/1‑148 (MQ=255)
                                         aGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATttgttg                                                                                                      <  2:713066/148‑1 (MQ=255)
                                          gAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGAGCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTtgttgt                                                                                                     <  2:3399218/148‑1 (MQ=255)
                                            aCAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGttt                                                                                                   >  1:1119819/1‑148 (MQ=255)
                                             cAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTg                                                                                                  <  2:1190006/148‑1 (MQ=255)
                                             cAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCAACGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTg                                                                                                  >  2:492021/1‑148 (MQ=255)
                                              aGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCAGTTGTTGTTTGc                                                                                                 >  2:1711546/1‑148 (MQ=255)
                                                      agaCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGAt                                                                                         <  2:2096253/148‑1 (MQ=255)
                                                        aCGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAg                                                                                       <  1:3144446/148‑1 (MQ=255)
                                                             cATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTgg                                                                                  <  1:817731/148‑1 (MQ=255)
                                                             cATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTgg                                                                                  >  1:1764067/1‑148 (MQ=255)
                                                             cATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTgg                                                                                  >  1:112654/1‑148 (MQ=255)
                                                               tGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgtt                                                                                >  2:1733660/1‑148 (MQ=255)
                                                                 ttCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgt                                                                              <  1:320278/148‑1 (MQ=255)
                                                                 ttCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgt                                                                              <  1:2624237/148‑1 (MQ=255)
                                                                  tCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGgttgtt                                                                             >  1:161897/1‑148 (MQ=255)
                                                                   cAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGttt                                                                            <  1:366737/148‑1 (MQ=255)
                                                                    aGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGtttt                                                                           >  2:1878430/1‑148 (MQ=255)
                                                                     gTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGttttt                                                                          >  1:1396394/1‑148 (MQ=255)
                                                                      tACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTg                                                                         <  1:15270/148‑1 (MQ=255)
                                                                       aCATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGc                                                                        >  1:202370/1‑148 (MQ=255)
                                                                       aCATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGc                                                                        <  2:1245966/148‑1 (MQ=255)
                                                                        cATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGcc                                                                       >  2:1068859/1‑148 (MQ=255)
                                                                        cATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGcc                                                                       <  1:3468685/148‑1 (MQ=255)
                                                                          tttttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCAt                                                                     >  2:169172/1‑148 (MQ=255)
                                                                          tttttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCAt                                                                     >  1:1442321/1‑148 (MQ=255)
                                                                            tttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCa                                                                   <  2:1670477/148‑1 (MQ=255)
                                                                             ttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCaa                                                                  >  1:576910/1‑148 (MQ=255)
                                                                             ttACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCaa                                                                  <  1:1370476/148‑1 (MQ=255)
                                                                               aCGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATa                                                                <  1:28538/148‑1 (MQ=255)
                                                                                   gCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACaaa                                                            >  1:2978976/1‑148 (MQ=255)
                                                                                   gCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACaaa                                                            <  2:402647/148‑1 (MQ=255)
                                                                                    cTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAg                                                           <  2:2354174/148‑1 (MQ=255)
                                                                                       aGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGaaa                                                        <  1:1083334/148‑1 (MQ=255)
                                                                                        gATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAat                                                       <  2:934856/148‑1 (MQ=255)
                                                                                         aTCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAata                                                      <  1:1987294/148‑1 (MQ=255)
                                                                                         aTCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAata                                                      >  1:1376686/1‑148 (MQ=255)
                                                                                          tctcGCTTGCCTGGGTTATCGGGCCACCTCTCGCTTATGAACTGGCAATGCGATTGAGTTTTAAAGTGATGTATCTCACAGCTGCCATCGCAGTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAatat                                                     >  2:3351560/1‑148 (MQ=255)
                                                                                          tctcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAatat                                                     <  1:1730467/148‑1 (MQ=255)
                                                                                           ctcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATAtt                                                    <  1:414753/148‑1 (MQ=255)
                                                                                            tcGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTc                                                   <  2:2417222/148‑1 (MQ=255)
                                                                                                ttGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGt                                               <  1:717628/148‑1 (MQ=255)
                                                                                                       ggTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTAcc                                        >  2:2956641/1‑148 (MQ=255)
                                                                                                            tCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAAcc                                   >  1:1717636/1‑148 (MQ=255)
                                                                                                             cGGGCCACCGCTCGCTTATGAACTGGCAATTGGATAGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTGGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAATAAATATTCCTGTCGTTACCCAAccc                                  >  1:2633950/1‑148 (MQ=255)
                                                                                                             cGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTTCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAAacc                                  >  1:1593996/1‑148 (MQ=255)
                                                                                                               ggCCACCGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGt                                <  2:52674/148‑1 (MQ=255)
                                                                                                                    ccGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGaaa                           >  2:245623/1‑148 (MQ=255)
                                                                                                                    ccGCTCGCTTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGaaa                           >  2:275394/1‑148 (MQ=255)
                                                                                                                     cgctcgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAAt                          <  2:1958994/148‑1 (MQ=255)
                                                                                                                         cgctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTa                      >  2:133322/1‑148 (MQ=255)
                                                                                                                          gctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTAc                     <  2:764047/148‑1 (MQ=255)
                                                                                                                          gctTATGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTAc                     <  1:2719360/148‑1 (MQ=255)
                                                                                                                              aTGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTc                 >  1:980132/1‑148 (MQ=255)
                                                                                                                              aTGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTc                 >  1:1705069/1‑148 (MQ=255)
                                                                                                                              aTGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTc                 >  2:918398/1‑148 (MQ=255)
                                                                                                                              aTGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTc                 >  1:980266/1‑148 (MQ=255)
                                                                                                                               tGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTcc                >  1:3380219/1‑148 (MQ=255)
                                                                                                                               tGAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTcc                >  2:2836253/1‑148 (MQ=255)
                                                                                                                                gAACTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTcca               >  2:1629568/1‑148 (MQ=255)
                                                                                                                                 aaCTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccac              >  2:398072/1‑148 (MQ=255)
                                                                                                                                  aCTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccacc             >  2:2590650/1‑148 (MQ=255)
                                                                                                                                   cTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccaccc            >  1:1521445/1‑148 (MQ=255)
                                                                                                                                   cTGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccaccc            <  1:319055/148‑1 (MQ=255)
                                                                                                                                    tGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccaccca           >  1:321415/1‑148 (MQ=255)
                                                                                                                                    tGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccaccca           >  1:42610/1‑148 (MQ=255)
                                                                                                                                    tGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCATGTCGTTACCCAACCCGTAGAAATTTTAACCTccaccca           >  2:2259567/1‑148 (MQ=255)
                                                                                                                                    tGGCAATGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATATGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTccaccca           >  2:494329/1‑148 (MQ=255)
                                                                                                                                          tGGGATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGaa     <  2:1693252/148‑1 (MQ=255)
                                                                                                                                           gggATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAg    >  2:1829030/1‑148 (MQ=255)
                                                                                                                                           gggATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAg    >  2:1828979/1‑148 (MQ=255)
                                                                                                                                           gggATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAg    <  2:1376686/148‑1 (MQ=255)
                                                                                                                                             gATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCg  >  2:216537/1‑148 (MQ=255)
                                                                                                                                             gATTGAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCg  <  2:1430323/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CAAACCCGCAAATGTTCGCCCTCGCCCGTGAACACGCCGACAGAACAGGCCGTGAGACGGTCATGTTCAGTACATTTTTACGTGCTCAGATCTCGCTTGCCTGGGTTATCGGGCCACCGCTCGCTTATGAACTGGCAATGGGATTTAGTTTTAAAGTGATGTATCTCACCGCTGCCATCGCATTTGTTGTTTGCGGACTGATAGTCTGGTTGTTTTTGCCATCAATACAAAGAAATATTCCTGTCGTTACCCAACCCGTAGAAATTTTACCCTCCACCCACAGGAAGCG  >  CP009273/3830674‑3830962

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑