Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 31,710 C→A 100% I298I (ATC→ATA)  carB → carbamoyl‑phosphate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00927331,7100CA97.5% 480.8 / 1.7 167I298I (ATC→ATA) carBcarbamoyl‑phosphate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base C (2/4);  new base A (98/63);  total (100/67)
Fisher's exact test for biased strand distribution p-value = 2.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 169 total aligned reads displayed.

CGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTC  >  CP009273/31566‑31856
                                                                                                                                                |                                                                                                                                                  
cgcgCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGaa                                                                                                                                                 <  2:2765219/148‑1 (MQ=255)
   gcCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATg                                                                                                                                              <  1:2734788/148‑1 (MQ=255)
       gCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAAcc                                                                                                                                          <  2:734774/148‑1 (MQ=255)
         ccAACCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCa                                                                                                                                        <  1:1622581/148‑1 (MQ=255)
          cAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAc                                                                                                                                       >  1:975464/1‑148 (MQ=255)
          cAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAc                                                                                                                                       >  1:1816864/1‑148 (MQ=255)
           aaaCGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCAcg                                                                                                                                      <  2:2416175/148‑1 (MQ=255)
              cGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCgt                                                                                                                                   >  2:3147479/1‑148 (MQ=255)
              cGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGAGTGTTATAGAAATGAACCCACGCgt                                                                                                                                   >  2:255110/1‑148 (MQ=255)
                 tGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTc                                                                                                                                <  1:2569763/148‑1 (MQ=255)
                 tGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGAGGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTc                                                                                                                                <  1:661895/148‑1 (MQ=255)
                     cGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGt                                                                                                                            <  1:1737213/148‑1 (MQ=255)
                     cGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGt                                                                                                                            <  1:3474650/148‑1 (MQ=255)
                       aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttc                                                                                                                          >  1:1137536/1‑148 (MQ=255)
                       aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttc                                                                                                                          >  2:3395820/1‑148 (MQ=255)
                       aCAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttc                                                                                                                          >  2:59253/1‑148 (MQ=255)
                        cAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttct                                                                                                                         >  1:346354/1‑148 (MQ=255)
                         aaaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttctt                                                                                                                        >  1:2837866/1‑148 (MQ=255)
                         aaaGAATATAAAATCATGCGTAAAGCCTCTATGGCGGTGCTGCGAGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCATTTTGCGGTGAACCCGAAAAAAGGTCGTCAGATTGTTATAGAAATTAACCCACGCGTGTCCCGttctt                                                                                                                        >  1:1948218/1‑148 (MQ=255)
                          aaGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGttcttc                                                                                                                       >  2:1905305/1‑148 (MQ=255)
                              atatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgcg                                                                                                                   >  1:282351/1‑148 (MQ=255)
                              atatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgcg                                                                                                                   >  1:2916747/1‑148 (MQ=255)
                              atatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgcg                                                                                                                   <  1:1195910/148‑1 (MQ=255)
                               tatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgcgc                                                                                                                  <  2:278350/148‑1 (MQ=255)
                               tatCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGgcgc                                                                                                                  >  2:796699/1‑148 (MQ=255)
                                atCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCt                                                                                                                 <  2:1396517/148‑1 (MQ=255)
                                  cAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTgg                                                                                                               >  1:3151088/1‑148 (MQ=255)
                                     aTCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGt                                                                                                            <  2:1989275/148‑1 (MQ=255)
                                         tGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaa                                                                                                        >  2:295214/1‑148 (MQ=255)
                                          gCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                       <  2:2113113/148‑1 (MQ=255)
                                          gCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                       <  1:1067479/148‑1 (MQ=255)
                                          gCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                       <  1:1067068/148‑1 (MQ=255)
                                          gCGGAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGGTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGaaa                                                                                                       <  2:2112790/148‑1 (MQ=255)
                                           cGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAg                                                                                                      <  1:1287773/148‑1 (MQ=255)
                                             tAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                    <  2:1790462/148‑1 (MQ=255)
                                             tAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGAGTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCg                                                                                                    >  2:2854185/1‑148 (MQ=255)
                                              aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                   <  2:3513656/148‑1 (MQ=255)
                                              aaCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGGCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                   <  2:2240270/148‑1 (MQ=255)
                                              aaCGCATCGCTGGCGGTGCGGCGTGAAATCGGCTTGGAACCGGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACTGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGa                                                                                                   <  2:687320/148‑1 (MQ=255)
                                               aCGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAc                                                                                                  >  2:477534/1‑148 (MQ=255)
                                                cGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGAcc                                                                                                 >  1:1221774/1‑148 (MQ=255)
                                                  ccTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCgg                                                                                               >  1:310444/1‑148 (MQ=255)
                                                  ccTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCgg                                                                                               >  1:310291/1‑148 (MQ=255)
                                                   cTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGt                                                                                              >  1:3315295/1‑148 (MQ=255)
                                                   cTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGt                                                                                              >  1:1404393/1‑148 (MQ=255)
                                                   cTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGt                                                                                              >  1:3313867/1‑148 (MQ=255)
                                                     cgttGGGGGTGGTGCGTTAAATCGGGTTTGAAACCGGTGGTTCCAACGTTCTGTTTGGGGTGAACCCGAAAAACGGTCGTCTTTTTGTTATATAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGttt                                                                                            <  2:282359/145‑1 (MQ=255)
                                                     cgttGGGGGTGCTGCGTTAAATGGGCGTTGAAACGGGTGTTTCAAACGTTCTTTTTGCGGTGAACCCGAAAAACGGTCGTCTTTTTGTTATATAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAACGCGACCGGttt                                                                                            <  2:282351/145‑1 (MQ=255)
                                                         ggCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCg                                                                                        >  1:1632508/1‑148 (MQ=255)
                                                            gggggtgcGTGAAATCGGCTTTGAACCCGGTGGTTCCAACGTTCTGTTTGCGGTCAACCCTAAAAACGGTCGTCTGTTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGAtt                                                                                     <  2:678239/143‑1 (MQ=255)
                                                                     ttaaaTCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGTTGAACCCGAAAAACGGTCGTCTGATTTTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTg                                                                            <  2:2217864/146‑1 (MQ=255)
                                                                     tGAAATCGGGGTTTAAACCGGTGGTTCCAAGGTTCTGTTTGCGGTGAACCCGAAAACGGGGCGTCTGATTTTTATAGAAATGACCCCCCGGGTGGCCCGTTCTTGGGCGGTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTg                                                                            <  2:740337/148‑1 (MQ=255)
                                                                     tGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTg                                                                            >  1:1114437/1‑148 (MQ=255)
                                                                     tGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTg                                                                            >  1:1114455/1‑148 (MQ=255)
                                                                      taaaGCGGCGTTGAAACGGGTGTTTCCAACGTTCATTTTGCGGTCAACCCGAAAAACGGTCGTCTGATTTTTATATAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTgg                                                                           <  2:1466422/147‑1 (MQ=255)
                                                                       aaaTCGGCGTTGAAACCGGTGGTTACAACGTTCAGTTTGAGGTGAACCCGAAAAACGGTCGTCTGATTGGTATAGAAATGAACCCACGCGTGTACCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTggc                                                                          >  1:1891928/1‑148 (MQ=255)
                                                                              cGTTCAAACGGGTGGTTCAAACGTTCTGTTGGCGGTGAACCCGAAAAAGGGTCGTCTTTTTTTTATAGAAATCAACCCCCGGGTGCCCCGTTCTTCGGCGGTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGaaa                                                                   <  1:1458088/148‑1 (MQ=255)
                                                                                ttGAACCCGGTGGTGCCACCGTTCTGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCCCGCGTGGCCGGTTCTTCGGCGCGGGCGCCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACt                                                                 <  2:229412/148‑1 (MQ=255)
                                                                                ttGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCTAAAACGGGTCGTCTGATTGTTATCGAAATGACCCCACGCGTGGCCCTTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACt                                                                 <  2:229268/148‑1 (MQ=255)
                                                                                 tGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTg                                                                >  2:2038913/1‑148 (MQ=255)
                                                                                     aCCGGTGGTTCCAAGGTTCTGTTTGCGGTCACCCCTAAAAAGGGTCTTCTTATTGTTATATAATTCAACCCACGCGGGTCCCGTTCTTGGGCGGGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTTGCgg                                                            <  2:1265732/148‑1 (MQ=255)
                                                                                     aCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCgg                                                            >  2:1710316/1‑148 (MQ=255)
                                                                                     aCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCgg                                                            >  1:63438/1‑148 (MQ=255)
                                                                                     aCCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCgg                                                            >  2:3447720/1‑148 (MQ=255)
                                                                                      ccGGTGGTTCCAACGTTCAGTTTGCGGTGAACACGAAAAACGGTCGTCGGATTGTTATAGAAATGAACCCACGCGTTTCCCGTTCTTCGGCGCAGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGt                                                           >  1:2584604/1‑148 (MQ=255)
                                                                                         gtggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggg                                                        >  1:730534/1‑148 (MQ=255)
                                                                                         gtggtTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTggg                                                        >  1:200075/1‑148 (MQ=255)
                                                                                          tgtttCCACCGTTCTGTTTGGGGTGACCCCCAAAACGGGTGGTCTTTTTTTTATATAATTGACCCCCCGGGTGGCCCGTTCTTCGGCGCGGGGGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGt                                                       <  2:1216936/145‑1 (MQ=255)
                                                                                          tggtGCCAACGTTCAGTTTGCGGTGAACCCCAAAACCGGTCGTGTGTTTGTTATCCAAATGACCCCCCGCGTGGCCCGTTCGGCGGCGCGGGCGTCCAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGt                                                       <  1:3173676/148‑1 (MQ=255)
                                                                                          gggtGCCACCGTTCTGTTGGCGGTGAACCCGAAAACCGGTGGTCTGATTTTTATAGAAATCAACCCCCGGGGGGCCCGTTCGGCGGGGCGGGCGACTAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGt                                                       <  2:1216894/147‑1 (MQ=255)
                                                                                                 aaCGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACAccc                                                >  1:86880/1‑148 (MQ=255)
                                                                                                  aCGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCt                                               >  1:107839/1‑148 (MQ=255)
                                                                                                   cGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTc                                              >  1:500236/1‑148 (MQ=255)
                                                                                                    gTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGGGGCCCGTTCTTCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcg                                             <  2:3501442/148‑1 (MQ=255)
                                                                                                      tCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTcgac                                           >  1:1279956/1‑148 (MQ=255)
                                                                                                         ttttGCGGTCACCCCAAAAAACGGTGTTCTTTTTTTTATACAAATCAACCCCCGGGTGGCCCGTTGTTGGGCGCTGGCGACGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGaa                                        <  1:477534/147‑1 (MQ=255)
                                                                                                         ttgtGCGGTCAACCCGAAAACCGGTCGTCTGATTGTTATATAAATCAACCCCCGCGGGGCCCGTTCTGCGGCGCTGGCGTCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGaa                                        <  2:3294404/145‑1 (MQ=255)
                                                                                                          tttGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                       >  1:3251365/1‑148 (MQ=255)
                                                                                                          tttGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                       >  1:1912865/1‑148 (MQ=255)
                                                                                                          tttGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                       >  2:1460793/1‑148 (MQ=255)
                                                                                                          tttGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAAc                                       >  1:2970131/1‑148 (MQ=255)
                                                                                                            tGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtg                                     >  2:2543494/1‑148 (MQ=255)
                                                                                                             atcttGAACCCCAAAAACGGTCGTGTTTTTTTTATATAAATGAACCCCCGCGGGGCCGGTTCTTCGGCGCGGGCGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtga                                    <  2:1784682/144‑1 (MQ=255)
                                                                                                                ttGAACCCGAAAAACGGTCTTGTGTTTGTTATAGAAATGAACCCACGCGTGTCCGGTTCTTCGGCGCGGGCGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACtgatga                                 <  2:2807298/147‑1 (MQ=255)
                                                                                                                  taaccataaaaaGGGTCGTTTGTTTTTTATATAAATGAACCCCGGGTTGTCCCGTTTTTCGGCGGTGGGGTCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAAc                               <  1:1382826/141‑1 (MQ=255)
                                                                                                                       ctaaaaaCGGTCGTGTTTTTTTTATACAAATCAACCCAGGGGTGTCCCTTTCTTCGGCGCTGGCGTCCAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACAt                          <  1:3147479/146‑1 (MQ=255)
                                                                                                                           aaaCGGTCGTCTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACt                      >  2:931335/1‑148 (MQ=255)
                                                                                                                               ggtgtgcTGTTTGTTATAGAAATGACCCCCCGGGGGGCCCGTTCTTGGGGGGGGGCGTCCAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCg                  <  1:3396624/142‑1 (MQ=255)
                                                                                                                               ggTCGTATTTTTTTTATAGAAATGAACCCCCGCGGGTCCCGGTGTGCGGCGGTGGGGTCTAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCg                  <  1:2010858/148‑1 (MQ=255)
                                                                                                                                    tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGt             >  2:2723296/1‑148 (MQ=255)
                                                                                                                                    tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGt             >  2:227504/1‑148 (MQ=255)
                                                                                                                                    tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGt             >  2:227065/1‑148 (MQ=255)
                                                                                                                                    tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGt             >  1:1860325/1‑148 (MQ=255)
                                                                                                                                    tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGt             >  1:83876/1‑148 (MQ=255)
                                                                                                                                    tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGt             >  2:294072/1‑148 (MQ=255)
                                                                                                                                    tcTGATTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGt             >  1:2513886/1‑148 (MQ=255)
                                                                                                                                        aTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTc         >  1:181317/1‑148 (MQ=255)
                                                                                                                                        aTTGTTATAGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTc         >  2:669336/1‑148 (MQ=255)
                                                                                                                                             tataGAAATGCACCCACGCGGGTCCCGTTCTGCGGCGCGGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcc    <  2:2816933/148‑1 (MQ=255)
                                                                                                                                               tCGAAATGAACCCACGCGGGTCCCGTTCTTCGGCGCGGGCGTCGAACGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGcctc  <  2:1116947/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                  
CGCGCCAGCCCAAACGCTGACCGACAAAGAATATCAAATCATGCGTAACGCCTCGATGGCGGTGCTGCGTGAAATCGGCGTTGAAACCGGTGGTTCCAACGTTCAGTTTGCGGTGAACCCGAAAAACGGTCGTCTGATTGTTATCGAAATGAACCCACGCGTGTCCCGTTCTTCGGCGCTGGCGTCGAAAGCGACCGGTTTCCCGATTGCTAAAGTGGCGGCGAAACTGGCGGTGGGTTACACCCTCGACGAACTGATGAACGACATCACTGGCGGACGTACTCCGGCCTC  >  CP009273/31566‑31856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑