Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 288,249 T→A 100% T137T (ACA→ACT)  yagK ← CP4‑6 prophage; conserved protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273288,2490TA99.5% 651.8 / ‑4.5 196T137T (ACA→ACT) yagKCP4‑6 prophage; conserved protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (89/106);  total (90/106)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

GGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGG  >  CP009273/288104‑288393
                                                                                                                                                 |                                                                                                                                                
gggaGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCagt                                                                                                                                                <  1:1660571/145‑1 (MQ=255)
 gTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCagtg                                                                                                                                               <  1:1437747/148‑1 (MQ=255)
 gTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCagtg                                                                                                                                               >  2:2220116/1‑148 (MQ=255)
  tAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGa                                                                                                                                              >  2:1153436/1‑148 (MQ=255)
    gTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatc                                                                                                                                            <  2:2135676/148‑1 (MQ=255)
    gTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatc                                                                                                                                            <  2:80826/148‑1 (MQ=255)
      ccAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatcat                                                                                                                                          >  1:24570/1‑148 (MQ=255)
       cAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatcatc                                                                                                                                         >  2:1722643/1‑148 (MQ=255)
       cAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGatcatc                                                                                                                                         <  2:3198219/148‑1 (MQ=255)
           cGGGTTAACAGCGCCTGTTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCctc                                                                                                                                     <  2:3284722/148‑1 (MQ=255)
           cGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCctc                                                                                                                                     <  2:2770429/148‑1 (MQ=255)
             ggTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTa                                                                                                                                   <  2:2370337/148‑1 (MQ=255)
              gTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTaa                                                                                                                                  >  1:596490/1‑148 (MQ=255)
               ttAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAg                                                                                                                                 <  2:2067931/148‑1 (MQ=255)
                tAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAgg                                                                                                                                <  1:1613345/148‑1 (MQ=255)
                  aCAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGta                                                                                                                              >  2:489648/1‑147 (MQ=255)
                   cAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTa                                                                                                                             <  2:258735/148‑1 (MQ=255)
                    aGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTAt                                                                                                                            <  1:2534120/148‑1 (MQ=255)
                        ccTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTc                                                                                                                        >  2:2902166/1‑148 (MQ=255)
                        ccTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTc                                                                                                                        >  2:1134963/1‑148 (MQ=255)
                         cTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTcc                                                                                                                       <  1:178043/148‑1 (MQ=255)
                           ggTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTg                                                                                                                     <  2:1354161/148‑1 (MQ=255)
                            gTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGa                                                                                                                    <  1:3172457/148‑1 (MQ=255)
                                 tCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCTTa                                                                                                               >  2:1677182/1‑148 (MQ=255)
                                     ctgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATcc                                                                                                           <  1:3355845/148‑1 (MQ=255)
                                     ctgGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATcc                                                                                                           <  1:1134963/148‑1 (MQ=255)
                                       ggAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATcccc                                                                                                         >  1:1102984/1‑148 (MQ=255)
                                        gAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCg                                                                                                        <  2:892029/148‑1 (MQ=255)
                                         aaGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGa                                                                                                       >  2:3469404/1‑148 (MQ=255)
                                              aGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAggtgg                                                                                                  <  1:2751297/148‑1 (MQ=255)
                                                tACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGgta                                                                                                >  1:475851/1‑148 (MQ=255)
                                                     ttGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAg                                                                                           >  1:2555337/1‑148 (MQ=255)
                                                     ttGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAg                                                                                           <  1:2145559/148‑1 (MQ=255)
                                                      tGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGc                                                                                          >  1:373401/1‑148 (MQ=255)
                                                        gtgtCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGt                                                                                        <  1:3016577/148‑1 (MQ=255)
                                                            cTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCttt                                                                                    >  2:2581540/1‑148 (MQ=255)
                                                            cTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCttt                                                                                    <  2:1901067/148‑1 (MQ=255)
                                                            cTAGCACATACTTACAGTTATCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACAAGGCTCCAGTGATCATCCCTCTAAGGATATCGACCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCttt                                                                                    >  2:909430/1‑148 (MQ=255)
                                                              aGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTttgt                                                                                  >  2:3109994/1‑148 (MQ=255)
                                                               gCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTttgtt                                                                                 >  2:1470768/1‑148 (MQ=255)
                                                               gCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTttgtt                                                                                 >  1:541037/1‑148 (MQ=255)
                                                                  caTACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGaa                                                                              >  2:3384971/1‑148 (MQ=255)
                                                                  caTACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGaa                                                                              >  1:2261128/1‑148 (MQ=255)
                                                                    tACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGaaca                                                                            <  2:2730977/148‑1 (MQ=255)
                                                                    tACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGaaca                                                                            <  1:1961237/148‑1 (MQ=255)
                                                                         aCAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAgg                                                                       >  1:171448/1‑148 (MQ=255)
                                                                          cAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGc                                                                      >  2:537831/1‑148 (MQ=255)
                                                                             ttttCAGGATAATGGACGAGACCAGTGTGATGGTCTCTCTCCATTCTCAGGGCTCTGTACCAGTCTCCAGGGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCTAGGTGGTTTTAAGCTTCTTTGTTGAACAACAGGCaaa                                                                   <  2:3257491/148‑1 (MQ=255)
                                                                             ttttCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCaaa                                                                   >  2:1991929/1‑148 (MQ=255)
                                                                             ttttCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCaaa                                                                   >  2:1621244/1‑148 (MQ=255)
                                                                                tCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAAtgt                                                                <  1:1721602/148‑1 (MQ=255)
                                                                                  aGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGa                                                              <  1:106179/148‑1 (MQ=255)
                                                                                  aGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGa                                                              <  1:271214/148‑1 (MQ=255)
                                                                                   ggAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGAt                                                             <  2:707521/148‑1 (MQ=255)
                                                                                    gAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATa                                                            <  1:1458354/148‑1 (MQ=255)
                                                                                    gAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATa                                                            >  2:3381218/1‑148 (MQ=255)
                                                                                    gAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCAGCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATa                                                            <  2:380203/148‑1 (MQ=255)
                                                                                     aaaaTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAg                                                           <  1:1349248/148‑1 (MQ=255)
                                                                                      aaaTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGt                                                          <  2:2959785/148‑1 (MQ=255)
                                                                                      aaaTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGt                                                          >  2:965084/1‑148 (MQ=255)
                                                                                      aaaTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGt                                                          <  2:1080118/148‑1 (MQ=255)
                                                                                       aaTGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTg                                                         <  1:2898308/148‑1 (MQ=255)
                                                                                         tGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGc                                                       <  1:2959706/148‑1 (MQ=255)
                                                                                          ggACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCa                                                      <  1:1477710/148‑1 (MQ=255)
                                                                                            accagaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCAtt                                                    >  2:2802485/1‑148 (MQ=255)
                                                                                              cagaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTa                                                  <  2:1243542/148‑1 (MQ=255)
                                                                                               agaccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTTAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTAc                                                 <  1:1006524/148‑1 (MQ=255)
                                                                                                 accagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCt                                               >  2:2648948/1‑148 (MQ=255)
                                                                                                  ccagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTg                                              <  1:3482545/148‑1 (MQ=255)
                                                                                                   cagGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGa                                             <  1:1434564/148‑1 (MQ=255)
                                                                                                     gggTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGAtt                                           >  2:450200/1‑148 (MQ=255)
                                                                                                      ggTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTc                                          <  2:3001943/148‑1 (MQ=255)
                                                                                                        tGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAg                                        <  1:3213901/148‑1 (MQ=255)
                                                                                                         gATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGa                                       <  2:3068892/148‑1 (MQ=255)
                                                                                                          aTCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGaa                                      <  2:2863016/148‑1 (MQ=255)
                                                                                                          aTCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGaa                                      >  1:310386/1‑148 (MQ=255)
                                                                                                           tCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAAt                                     >  1:1749627/1‑148 (MQ=255)
                                                                                                            cGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATa                                    >  1:2409057/1‑148 (MQ=255)
                                                                                                               ctctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATActc                                 <  1:3257860/148‑1 (MQ=255)
                                                                                                               ctctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATActc                                 <  2:1301400/148‑1 (MQ=255)
                                                                                                               ctctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATActc                                 >  1:2556771/1‑148 (MQ=255)
                                                                                                                tctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATActct                                <  1:280211/148‑1 (MQ=255)
                                                                                                                tctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATActct                                <  1:279643/148‑1 (MQ=255)
                                                                                                                 ctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCtt                               >  2:944433/1‑148 (MQ=255)
                                                                                                                 ctctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCtt                               <  2:1292272/148‑1 (MQ=255)
                                                                                                                  tctcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTCAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCttt                              <  1:2220116/148‑1 (MQ=255)
                                                                                                                    tcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCg                            >  1:2694548/1‑148 (MQ=255)
                                                                                                                    tcCAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCg                            >  2:542618/1‑148 (MQ=255)
                                                                                                                      cAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGcc                          <  2:473506/148‑1 (MQ=255)
                                                                                                                      cAGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGcc                          <  2:541037/148‑1 (MQ=255)
                                                                                                                       aGTCTCAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGccc                         >  1:3322122/1‑148 (MQ=255)
                                                                                                                         tctcAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCat                       >  1:2358210/1‑148 (MQ=255)
                                                                                                                            cAGGGCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCAtata                    <  2:1716422/148‑1 (MQ=255)
                                                                                                                               ggCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATataata                 >  2:2967404/1‑148 (MQ=255)
                                                                                                                               ggCGCTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATataata                 <  2:1522364/148‑1 (MQ=255)
                                                                                                                                 cgcTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATaataaa               <  1:904803/148‑1 (MQ=255)
                                                                                                                                 cgcTGTACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATaataaa               <  2:2082332/148‑1 (MQ=255)
                                                                                                                                      tACCAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAg          >  2:269910/1‑148 (MQ=255)
                                                                                                                                        ccAGGCTCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAggg        <  2:1102984/148‑1 (MQ=255)
                                                                                                                                              tCCAGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACgg  <  2:2553762/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
GGTAGTCCAGTCGGGTTAACAGCGCCTGGTAGTTCTGCTGGAAGTCAGTACTATTGGTGTCTAGCACATACTTACAGTTTTCAGGAAAATGGACCAGACCAGGGTGATCGTCTCTCTCCAGTCTCAGGGCGCTGTACCAGGCTCCTGTGATCATCCCTCTAAGGTTATCGTCCTGATCGTAATCCCCGAGGTGGTAGTAAGCGTCTTTGTTGAACAACAGGCAAATGTGATAGTGGCATTTACCTGATTCAGAATACTCTTTCGCCCATATAATAAATAAAGGGCAACGG  >  CP009273/288104‑288393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑