Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,865,120 C→A 100% intergenic (‑30/+90) dgoT ← / ← dgoD D‑galactonate transporter/galactonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,865,1200CA95.7% 724.0 / 17.1 242intergenic (‑30/+90)dgoT/dgoDD‑galactonate transporter/galactonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (2/9);  new base A (112/119);  total (114/128)
Fisher's exact test for biased strand distribution p-value = 6.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 242 total aligned reads displayed.

AAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTCC  >  CP009273/3864976‑3865264
                                                                                                                                                |                                                                                                                                                
aaGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGaa                                                                                                                                               >  1:3503236/1‑148 (MQ=255)
  gCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGc                                                                                                                                             >  1:957734/1‑148 (MQ=255)
    cACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCg                                                                                                                                           <  1:3029362/148‑1 (MQ=255)
              ttGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGc                                                                                                                                 <  1:2690479/148‑1 (MQ=255)
               tGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGcc                                                                                                                                <  2:2049238/148‑1 (MQ=255)
               tGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCAAGATTTTGGGCATAGAGAAGCCGCTCACCATGcc                                                                                                                                >  2:2788635/1‑148 (MQ=255)
               tGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCAAGATTTTGGGCATAGAGAAGCCGCTCACCATGcc                                                                                                                                >  2:1037068/1‑148 (MQ=255)
                  cgcgGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTg                                                                                                                             <  2:2809846/148‑1 (MQ=255)
                   gcgTTCGACATAAAAAATGACTACCGTAATAAACATCATCACCAGCTTAAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGt                                                                                                                            <  2:1636410/148‑1 (MQ=255)
                         gACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTa                                                                                                                      <  2:1059322/148‑1 (MQ=255)
                          aCATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTAc                                                                                                                     >  2:1548522/1‑148 (MQ=255)
                           cATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACa                                                                                                                    >  2:597991/1‑148 (MQ=255)
                           cATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACa                                                                                                                    >  2:226357/1‑148 (MQ=255)
                               aCAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAgg                                                                                                                >  2:2605086/1‑148 (MQ=255)
                               aCAAATGACTACCGTAAGAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAgg                                                                                                                <  2:1303752/148‑1 (MQ=255)
                                 aaaTGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGt                                                                                                              <  1:127342/148‑1 (MQ=255)
                                    tGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTtat                                                                                                           >  2:277537/1‑148 (MQ=255)
                                    tGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTtat                                                                                                           >  1:2198463/1‑148 (MQ=255)
                                    tGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTtat                                                                                                           >  1:2007659/1‑148 (MQ=255)
                                     gACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTtata                                                                                                          >  2:236788/1‑148 (MQ=255)
                                      aCTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTACTTTAAAGAGGGGTatat                                                                                                         >  1:5404/1‑148 (MQ=255)
                                      aCTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTatat                                                                                                         >  2:266459/1‑148 (MQ=255)
                                      aCTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTatat                                                                                                         >  1:24340/1‑148 (MQ=255)
                                       cTACCGTAATAAAGCTCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATAtt                                                                                                        <  1:3419350/148‑1 (MQ=255)
                                       cTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATAtt                                                                                                        <  2:2827854/148‑1 (MQ=255)
                                       cTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATAtt                                                                                                        >  1:871083/1‑148 (MQ=255)
                                        tACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATAttt                                                                                                       >  1:2531446/1‑148 (MQ=255)
                                         aCCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATAtttt                                                                                                      >  1:84495/1‑148 (MQ=255)
                                          ccGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATAttttt                                                                                                     >  1:1422328/1‑148 (MQ=255)
                                          ccGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATAttttt                                                                                                     >  1:1644474/1‑148 (MQ=255)
                                              aataaAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATATCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTAt                                                                                                 <  2:1474657/148‑1 (MQ=255)
                                              aataaAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTAt                                                                                                 <  2:1699243/148‑1 (MQ=255)
                                                taaAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTg                                                                                               >  2:2423130/1‑148 (MQ=255)
                                                taaAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTg                                                                                               >  1:3239439/1‑148 (MQ=255)
                                                 aaaGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGc                                                                                              <  2:821214/148‑1 (MQ=255)
                                                 aaaGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGc                                                                                              >  1:2659746/1‑148 (MQ=255)
                                                   aGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTg                                                                                            >  2:159001/1‑148 (MQ=255)
                                                    gATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGt                                                                                           <  1:1103606/148‑1 (MQ=255)
                                                    gATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGt                                                                                           >  1:1535322/1‑148 (MQ=255)
                                                      tcatcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGttt                                                                                         <  2:3352272/148‑1 (MQ=255)
                                                      tcatcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGttt                                                                                         >  1:2400090/1‑148 (MQ=255)
                                                       catcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGtttt                                                                                        <  1:762449/148‑1 (MQ=255)
                                                         tcaCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAg                                                                                      >  2:3017493/1‑148 (MQ=255)
                                                          caCCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATCTTTTTGTATTGCTGTTTTAGt                                                                                     >  2:2501511/1‑148 (MQ=255)
                                                            ccAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGc                                                                                   <  1:506694/148‑1 (MQ=255)
                                                             cAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGcc                                                                                  <  2:1174682/148‑1 (MQ=255)
                                                               gCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCg                                                                                >  2:2343500/1‑148 (MQ=255)
                                                               gCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCg                                                                                >  1:214636/1‑148 (MQ=255)
                                                               gCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCg                                                                                >  1:2810544/1‑148 (MQ=255)
                                                                cGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGa                                                                               >  1:331625/1‑148 (MQ=255)
                                                                   cAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGa                                                                            <  2:438112/148‑1 (MQ=255)
                                                                        aaCGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGc                                                                       <  1:118873/148‑1 (MQ=255)
                                                                        aaCGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGc                                                                       >  2:2548540/1‑148 (MQ=255)
                                                                        aaCGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGc                                                                       <  1:1993185/148‑1 (MQ=255)
                                                                         aCGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCt                                                                      <  1:2316989/148‑1 (MQ=255)
                                                                              gACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGt                                                                 <  2:2168084/148‑1 (MQ=255)
                                                                                cGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTgg                                                               <  1:586200/148‑1 (MQ=255)
                                                                                cGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTgg                                                               <  2:749854/148‑1 (MQ=255)
                                                                                 gCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGc                                                              <  1:3138924/148‑1 (MQ=255)
                                                                                        cTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAAtt                                                       >  1:1220614/1‑148 (MQ=255)
                                                                                          ttGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCTATGAGGGGCTTACGTGGCAGGAATTAc                                                     >  1:2172615/1‑148 (MQ=255)
                                                                                          ttGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTAc                                                     <  1:3112010/148‑1 (MQ=255)
                                                                                          ttGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGACCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTAc                                                     >  2:3167485/1‑148 (MQ=255)
                                                                                           tgctgcATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTAcc                                                    <  1:552356/148‑1 (MQ=255)
                                                                                               gcATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCCTAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGATGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCActc                                                >  2:1593415/1‑148 (MQ=255)
                                                                                               gcATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCActc                                                >  1:2103386/1‑148 (MQ=255)
                                                                                                 aTTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTg                                              <  2:1857430/148‑1 (MQ=255)
                                                                                                    aaCGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTa                                           >  1:1327107/1‑148 (MQ=255)
                                                                                                      cGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACg                                         <  2:3077888/148‑1 (MQ=255)
                                                                                                           atatCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGtt                                    >  1:1182408/1‑148 (MQ=255)
                                                                                                             atCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTAt                                  <  2:65738/148‑1 (MQ=255)
                                                                                                               ccATAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCt                                <  1:2962777/148‑1 (MQ=255)
                                                                                                                 aTAGCGATCTTTCTCCAGATTTTGGTCATAGAGATTCCGCTCACCATGCCCTGTAATTTACAGATGGTTATATTTTTGTATTGCTGTTTTAGTGCCAGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTc                              <  2:2514467/148‑1 (MQ=255)
                                                                                                                 aTAGCGAGCTTTCTCCAGATTGTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTc                              <  2:3467741/148‑1 (MQ=255)
                                                                                                                  tAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCa                             <  2:800939/148‑1 (MQ=255)
                                                                                                                  tAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCa                             <  1:1200998/148‑1 (MQ=255)
                                                                                                                  tAGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCa                             <  1:236788/148‑1 (MQ=255)
                                                                                                                   aGCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCAt                            >  1:2573070/1‑148 (MQ=255)
                                                                                                                    gCGAGCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATg                           <  2:1344514/148‑1 (MQ=255)
                                                                                                                        gCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGc                       >  2:531672/1‑148 (MQ=255)
                                                                                                                        gCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGc                       >  2:2888268/1‑148 (MQ=255)
                                                                                                                        gCTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGc                       >  1:704698/1‑148 (MQ=255)
                                                                                                                         cTTTCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGcc                      <  1:1369003/148‑1 (MQ=255)
                                                                                                                          tttCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCa                     >  2:1347563/1‑148 (MQ=255)
                                                                                                                           ttCTCCAGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCag                    <  2:692831/148‑1 (MQ=255)
                                                                                                                           ttCTCCAGATTGTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCag                    <  2:2091133/148‑1 (MQ=255)
                                                                                                                                 aGATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGa              <  2:2573070/148‑1 (MQ=255)
                                                                                                                                  gATTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGAt             >  2:1435092/1‑148 (MQ=255)
                                                                                                                                   aTTTTGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGAtt            >  2:1934280/1‑148 (MQ=255)
                                                                                                                                     tttGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTAc          >  1:183929/1‑148 (MQ=255)
                                                                                                                                      ttGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACg         <  1:1913336/148‑1 (MQ=255)
                                                                                                                                       tGGGCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGc        <  1:2308638/148‑1 (MQ=255)
                                                                                                                                         ggCATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCa      >  2:1208216/1‑148 (MQ=255)
                                                                                                                                           cATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGt    <  2:3373085/148‑1 (MQ=255)
                                                                                                                                           cATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGt    <  1:2793162/148‑1 (MQ=255)
                                                                                                                                           cATAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGt    <  1:1900709/148‑1 (MQ=255)
                                                                                                                                            aTAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTc   <  2:1563115/148‑1 (MQ=255)
                                                                                                                                             tAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTcc  >  1:2255851/1‑148 (MQ=255)
                                                                                                                                             tAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTcc  <  1:2886120/148‑1 (MQ=255)
                                                                                                                                             tAGAGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTcc  >  1:1437433/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                                
AAGCCACGGCCAGGTTGGCGCGGTCGACATAACAAATGACTACCGTAATAAAGATCATCACCAGCGTCAGATAACGCCGACGCCCCGGCTTTGCTGCATTAACGGGAATATCCATAGCGAGCTTTCTCCAGATTTTGGGCATAGCGAAGCCGCTCACCATGCCCTGTAATTTACAGAGGGTTATATTTTTGTATTGCTGTTTTAGTGCCCGATGAGGGGCTTACGTGGCAGGAATTACCACTCTGCTACGCTGTTATCTTCATGACGCCAGAGCGGATTACGCCAGTCC  >  CP009273/3864976‑3865264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑