Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,865,863 T→A 100% T166S (ACC→TCC)  dgoD ← galactonate dehydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,865,8630TA99.6% 744.5 / ‑4.7 224T166S (ACC→TCC) dgoDgalactonate dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base A (97/126);  total (97/127)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 224 total aligned reads displayed.

CAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGCCAACCCAACT  >  CP009273/3865720‑3866007
                                                                                                                                               |                                                                                                                                                
cAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTa                                                                                                                                              >  1:899685/1‑148 (MQ=255)
  gCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAc                                                                                                                                            <  1:3198018/148‑1 (MQ=255)
  gCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAc                                                                                                                                            <  1:3198030/148‑1 (MQ=255)
  gCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAc                                                                                                                                            <  1:795664/148‑1 (MQ=255)
  gCACCGGCTCCTCAATAAACAGCGGGCGATACGGATCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACTCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAc                                                                                                                                            >  2:1399976/1‑148 (MQ=255)
    aCCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccg                                                                                                                                          <  1:2065356/148‑1 (MQ=255)
    aCCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccg                                                                                                                                          <  1:486171/148‑1 (MQ=255)
     ccGGCTCCTCAATAACCAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAccgc                                                                                                                                         <  1:1050808/148‑1 (MQ=255)
            ctcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCt                                                                                                                                  >  1:2094104/1‑148 (MQ=255)
             tcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTa                                                                                                                                 <  1:1347843/148‑1 (MQ=255)
             tcAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTa                                                                                                                                 <  2:2804571/148‑1 (MQ=255)
              cAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTAc                                                                                                                                >  1:3415905/1‑148 (MQ=255)
                  aaaCAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACcgcg                                                                                                                            >  2:1123777/1‑148 (MQ=255)
                   aaCAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACcgcgc                                                                                                                           >  1:1866741/1‑148 (MQ=255)
                      aGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCggg                                                                                                                        >  1:1398267/1‑148 (MQ=255)
                       gCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGa                                                                                                                       >  2:1606377/1‑148 (MQ=255)
                        cGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAg                                                                                                                      >  1:2001015/1‑148 (MQ=255)
                          ggCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGtt                                                                                                                    >  1:875762/1‑148 (MQ=255)
                           gCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATCTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTa                                                                                                                   <  2:576153/148‑1 (MQ=255)
                            cGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTAt                                                                                                                  <  1:2973725/148‑1 (MQ=255)
                               tACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCaa                                                                                                               >  1:597406/1‑148 (MQ=255)
                                aCGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAAt                                                                                                              <  1:1462347/148‑1 (MQ=255)
                                aCGGCTCCAGTTCTTTAAACAGCCCTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAAt                                                                                                              <  1:1501039/148‑1 (MQ=255)
                                 cGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAAtt                                                                                                             >  2:1057609/1‑148 (MQ=255)
                                 cGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAAtt                                                                                                             <  1:1138833/148‑1 (MQ=255)
                                 cGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAAtt                                                                                                             >  1:2373254/1‑148 (MQ=255)
                                 cGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAAtt                                                                                                             >  1:2549926/1‑148 (MQ=255)
                                 cGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAAtt                                                                                                             <  1:1138712/148‑1 (MQ=255)
                                    cTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGc                                                                                                          <  1:912181/148‑1 (MQ=255)
                                    cTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGc                                                                                                          >  1:2859038/1‑148 (MQ=255)
                                    cTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGc                                                                                                          <  1:2273600/148‑1 (MQ=255)
                                    cTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGc                                                                                                          <  1:2273517/148‑1 (MQ=255)
                                      ccAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGccc                                                                                                        <  2:1659996/148‑1 (MQ=255)
                                       cAGTTCTTTAATCATCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGcccc                                                                                                       <  2:2961238/148‑1 (MQ=255)
                                        aGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCa                                                                                                      <  2:2113466/148‑1 (MQ=255)
                                            cTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttc                                                                                                  >  2:654759/1‑148 (MQ=255)
                                            cTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttc                                                                                                  >  2:613186/1‑148 (MQ=255)
                                             tttAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGttct                                                                                                 <  2:2668262/148‑1 (MQ=255)
                                                aaTCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTca                                                                                              <  1:1488154/148‑1 (MQ=255)
                                                  tCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTcaca                                                                                            <  2:2596713/148‑1 (MQ=255)
                                                       aCTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGt                                                                                       <  1:169719/148‑1 (MQ=255)
                                                         tttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTCCCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTc                                                                                     >  1:2948098/1‑148 (MQ=255)
                                                         tttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTc                                                                                     <  2:1553906/148‑1 (MQ=255)
                                                         tttCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTc                                                                                     <  2:2505624/148‑1 (MQ=255)
                                                            cGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGt                                                                                  <  1:674169/148‑1 (MQ=255)
                                                            cGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGt                                                                                  <  1:2153724/148‑1 (MQ=255)
                                                             gCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGtt                                                                                 <  1:3108015/148‑1 (MQ=255)
                                                               cATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTg                                                                               >  2:1490282/1‑148 (MQ=255)
                                                               cATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTg                                                                               >  2:755931/1‑148 (MQ=255)
                                                                aTCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGa                                                                              >  1:1789474/1‑148 (MQ=255)
                                                                 tCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGaa                                                                             <  1:1271202/148‑1 (MQ=255)
                                                                    gcgcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGt                                                                          <  1:2043485/148‑1 (MQ=255)
                                                                    gcgcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGt                                                                          <  2:3408340/148‑1 (MQ=255)
                                                                     cgcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTa                                                                         <  2:3353757/148‑1 (MQ=255)
                                                                     cgcgcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTa                                                                         >  1:471054/1‑148 (MQ=255)
                                                                        gcTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCg                                                                      <  2:3058932/148‑1 (MQ=255)
                                                                         cTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGa                                                                     <  2:2016553/148‑1 (MQ=255)
                                                                         cTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGa                                                                     <  2:2437743/148‑1 (MQ=255)
                                                                          tGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGaa                                                                    <  2:1352948/148‑1 (MQ=255)
                                                                           gACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAg                                                                   <  2:377148/148‑1 (MQ=255)
                                                                            aCGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGc                                                                  >  2:692047/1‑148 (MQ=255)
                                                                            aCGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAAACGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTGTGAAGGTATCGAAGc                                                                  >  1:2909887/1‑148 (MQ=255)
                                                                                 aCCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGAtt                                                             <  2:1100493/148‑1 (MQ=255)
                                                                                 aCCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGAtt                                                             <  1:1338290/148‑1 (MQ=255)
                                                                                    gTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTcg                                                          <  2:380237/148‑1 (MQ=255)
                                                                                      ggAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTcgcg                                                        <  1:122067/148‑1 (MQ=255)
                                                                                      ggAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTcgcg                                                        >  2:2879607/1‑148 (MQ=255)
                                                                                       gAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGt                                                       <  1:1174457/148‑1 (MQ=255)
                                                                                       gAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGt                                                       >  2:3012164/1‑148 (MQ=255)
                                                                                         aaTCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAg                                                     >  2:539013/1‑148 (MQ=255)
                                                                                         aaTCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAg                                                     <  1:1862377/148‑1 (MQ=255)
                                                                                         aaTCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAg                                                     <  1:860063/148‑1 (MQ=255)
                                                                                          aTCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGc                                                    >  2:2758027/1‑148 (MQ=255)
                                                                                           tCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCg                                                   >  2:556533/1‑148 (MQ=255)
                                                                                            cAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGt                                                  <  2:100669/148‑1 (MQ=255)
                                                                                            cAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGt                                                  <  2:1876279/148‑1 (MQ=255)
                                                                                             aaGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGtt                                                 <  2:2114059/148‑1 (MQ=255)
                                                                                              aGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGttt                                                >  2:454543/1‑148 (MQ=255)
                                                                                              aGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGttt                                                <  1:3404436/148‑1 (MQ=255)
                                                                                              aGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGttt                                                >  1:2833767/1‑148 (MQ=255)
                                                                                                 ccAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTaa                                             >  1:2251151/1‑148 (MQ=255)
                                                                                                   aaaCTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATg                                           >  2:3101644/1‑148 (MQ=255)
                                                                                                      cTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCg                                        >  2:89910/1‑148 (MQ=255)
                                                                                                        cAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTc                                      >  2:179420/1‑148 (MQ=255)
                                                                                                         aaTCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCg                                     <  2:2094104/148‑1 (MQ=255)
                                                                                                          aTCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTCGCGTTTTAATGCCGTCGa                                    >  2:3375012/1‑148 (MQ=255)
                                                                                                          aTCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGa                                    <  2:1866741/148‑1 (MQ=255)
                                                                                                          aTCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGa                                    <  2:313678/148‑1 (MQ=255)
                                                                                                           tCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGAt                                   >  2:226144/1‑148 (MQ=255)
                                                                                                           tCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGAt                                   >  2:1245960/1‑148 (MQ=255)
                                                                                                           tCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGAt                                   <  1:754349/148‑1 (MQ=255)
                                                                                                             tGATTGCCAAAAGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATaa                                 <  1:2915778/148‑1 (MQ=255)
                                                                                                                     aaaaGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATccgcc                         <  2:3250591/148‑1 (MQ=255)
                                                                                                                     aaaaGCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATccgcc                         <  2:134125/148‑1 (MQ=255)
                                                                                                                         gCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGAc                     >  2:310653/1‑148 (MQ=255)
                                                                                                                         gCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGAc                     >  1:3194286/1‑148 (MQ=255)
                                                                                                                         gCTTCACGAATTTGTGCCACGGAGTTAACCGCCGCAGCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCGTCACAACCGTTCAGGTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGAc                     >  2:3453858/1‑148 (MQ=255)
                                                                                                                                  aTTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATcgccgc            >  1:1634070/1‑148 (MQ=255)
                                                                                                                                  aTTTGTGCCACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATcgccgc            >  1:307681/1‑148 (MQ=255)
                                                                                                                                          cACGGAGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGccaacccaa    <  1:363827/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CAGCACCGGCTCCTCAATAAACAGCGGGCGATACGGCTCCAGTTCTTTAATCAGCACTTTCGCCATCGGCGCGCTGACGCGACCGTGGAAATCAAGACCAAACTCAATCTGATTGCCAAAAGCTTCACGAATTTGTGCCACGGTGTTAACCGCCGCATCTACCGCGCGGGAGTTATCAATTAGCCCCAGTTCTTCACAACCGTTCAGTTTGAAGGTATCGAAGCCGATTTCGCGTAGCGTTTTAATGCCGTCGATAACATCCGCCGGACGATCGCCGCCAACCCAACT  >  CP009273/3865720‑3866007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑