Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,873,695 C→A 100% R296L (CGG→CTG)  recF ← gap repair protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,873,6950CA98.5% 632.4 / 0.5 199R296L (CGG→CTG) recFgap repair protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (100/95);  total (102/97)
Fisher's exact test for biased strand distribution p-value = 6.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 205 total aligned reads displayed.

TTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCG  >  CP009273/3873551‑3873835
                                                                                                                                                |                                                                                                                                            
ttttCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACAcagcc                                                                                                                                           >  1:3457462/1‑148 (MQ=255)
  ttCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACcgccgc                                                                                                                                         <  2:1605551/148‑1 (MQ=255)
   tCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAgccgcc                                                                                                                                        >  1:60907/1‑148 (MQ=255)
    cGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGccgccc                                                                                                                                       >  2:2911596/1‑148 (MQ=255)
       ccGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCt                                                                                                                                    >  1:2172893/1‑148 (MQ=255)
       ccGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCt                                                                                                                                    >  2:2313535/1‑148 (MQ=255)
       ccGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCt                                                                                                                                    >  2:3364210/1‑148 (MQ=255)
       ccGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCt                                                                                                                                    <  1:1604571/148‑1 (MQ=255)
        cGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCtt                                                                                                                                   >  1:2613655/1‑148 (MQ=255)
        cGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCtt                                                                                                                                   >  1:204447/1‑148 (MQ=255)
          aCATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTc                                                                                                                                 >  1:414585/1‑148 (MQ=255)
          aCATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTc                                                                                                                                 <  2:530175/148‑1 (MQ=255)
          aCATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTc                                                                                                                                 >  1:179067/1‑148 (MQ=255)
           cATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCa                                                                                                                                <  1:1518289/148‑1 (MQ=255)
           cATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCa                                                                                                                                >  2:1654021/1‑148 (MQ=255)
           cATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCa                                                                                                                                <  2:3492960/148‑1 (MQ=255)
             tGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACg                                                                                                                              >  1:1063294/1‑148 (MQ=255)
             tGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACg                                                                                                                              <  1:1415524/148‑1 (MQ=255)
              gTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCCCTTTAACgg                                                                                                                             >  1:379415/1‑148 (MQ=255)
               tCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACggg                                                                                                                            <  2:2348134/148‑1 (MQ=255)
                cTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGt                                                                                                                           >  2:2116632/1‑148 (MQ=255)
                  ataACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGa                                                                                                                         >  1:853988/1‑148 (MQ=255)
                   taACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAg                                                                                                                        <  2:2347369/148‑1 (MQ=255)
                    aaCGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAgg                                                                                                                       >  1:1075045/1‑148 (MQ=255)
                    aaCGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAgg                                                                                                                       >  1:1074872/1‑148 (MQ=255)
                     aCGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGa                                                                                                                      >  2:312050/1‑148 (MQ=255)
                      cGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGac                                                                                                                     >  2:1636791/1‑147 (MQ=255)
                      cGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGaa                                                                                                                     <  2:3090573/148‑1 (MQ=255)
                        tgtTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAAct                                                                                                                   <  1:1044167/148‑1 (MQ=255)
                          ttCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGCActct                                                                                                                 >  1:3330764/1‑148 (MQ=255)
                            cAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCGTTCACGGGTGAGGAACTCTcc                                                                                                               >  2:800743/1‑148 (MQ=255)
                             aGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCt                                                                                                              <  1:3133102/148‑1 (MQ=255)
                              gCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCtt                                                                                                             >  2:2839769/1‑148 (MQ=255)
                               cACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTg                                                                                                            <  2:1586804/148‑1 (MQ=255)
                               cACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTg                                                                                                            <  2:2069241/148‑1 (MQ=255)
                               cACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTg                                                                                                            <  2:296654/148‑1 (MQ=255)
                                     tCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAg                                                                                                      <  2:1080800/148‑1 (MQ=255)
                                     tCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTAATCAACAAGCGCAGAGGCAAAATCATCTATCAGGTAGAGAAACAGCCCCCCCCATTCACCGGTGAGGAACTCTCCTTGCGCCAg                                                                                                      >  1:107868/1‑148 (MQ=255)
                                       gcgcTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGAc                                                                                                    <  1:1374494/148‑1 (MQ=255)
                                        cgcTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACg                                                                                                   <  1:188269/148‑1 (MQ=255)
                                        cgcTGACAAAGACCTGTGATTGCGTCGATTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACg                                                                                                   <  1:187701/148‑1 (MQ=255)
                                          cTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTa                                                                                                 <  2:2091579/148‑1 (MQ=255)
                                             aCAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAgg                                                                                              <  1:196324/148‑1 (MQ=255)
                                              cAAAGACCTGTGATTGCGTCGCTTTTAAGCGGGTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGTGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGCACTCTCCTTGCGCCAGACGTTAGgc                                                                                             >  1:304519/1‑148 (MQ=255)
                                              cAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgc                                                                                             <  2:1874153/148‑1 (MQ=255)
                                              cAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgc                                                                                             >  2:673873/1‑148 (MQ=255)
                                               aaaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcg                                                                                            >  1:135919/1‑148 (MQ=255)
                                               aaaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcg                                                                                            >  1:1909591/1‑148 (MQ=255)
                                                aaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcgc                                                                                           >  1:2477316/1‑148 (MQ=255)
                                                aaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcgc                                                                                           >  2:341013/1‑148 (MQ=255)
                                                aaGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGgcgc                                                                                           >  2:791728/1‑148 (MQ=255)
                                                   aCCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGcaca                                                                                        <  1:3412694/148‑1 (MQ=255)
                                                   aCCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGcaca                                                                                        <  2:1850380/148‑1 (MQ=255)
                                                    ccTGTGAGTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACAt                                                                                       <  2:2001456/148‑1 (MQ=255)
                                                       gtgATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCaa                                                                                    <  1:219576/148‑1 (MQ=255)
                                                         gATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACa                                                                                  <  2:382604/148‑1 (MQ=255)
                                                           ttGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGc                                                                                <  2:1382698/148‑1 (MQ=255)
                                                              cGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTa                                                                             >  2:2626625/1‑148 (MQ=255)
                                                              cGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTa                                                                             <  2:2543837/148‑1 (MQ=255)
                                                              cGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTa                                                                             >  2:2394484/1‑148 (MQ=255)
                                                              cGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTa                                                                             <  2:390787/148‑1 (MQ=255)
                                                              cGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCGTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTa                                                                             >  2:614118/1‑148 (MQ=255)
                                                                tCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAg                                                                           <  2:2753124/148‑1 (MQ=255)
                                                                   cTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTg                                                                        <  1:478320/148‑1 (MQ=255)
                                                                    ttttAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGc                                                                       >  2:1228726/1‑148 (MQ=255)
                                                                         aGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCAcg                                                                  >  2:28893/1‑148 (MQ=255)
                                                                           cGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCAcgcg                                                                >  1:3247205/1‑148 (MQ=255)
                                                                           cGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCAcgcg                                                                >  1:96370/1‑148 (MQ=255)
                                                                               tGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATaa                                                            <  1:2285535/148‑1 (MQ=255)
                                                                                    agcagcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTAt                                                       <  1:2620258/148‑1 (MQ=255)
                                                                                        gcCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTc                                                   >  1:708374/1‑148 (MQ=255)
                                                                                         cccGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTcc                                                  <  2:708374/148‑1 (MQ=255)
                                                                                             cgACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCg                                              >  2:3223442/1‑148 (MQ=255)
                                                                                                cGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGgcg                                           >  2:639396/1‑148 (MQ=255)
                                                                                                     atcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCg                                      >  1:1154669/1‑148 (MQ=255)
                                                                                                      tcatcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGt                                     >  2:1187614/1‑148 (MQ=255)
                                                                                                       catcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTc                                    >  1:1240649/1‑148 (MQ=255)
                                                                                                        atcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCg                                   >  1:2975119/1‑148 (MQ=255)
                                                                                                        atcaAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCg                                   <  1:2597813/148‑1 (MQ=255)
                                                                                                            aGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGgcgc                               >  2:725409/1‑148 (MQ=255)
                                                                                                             gCTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGcgcg                              >  1:2737618/1‑148 (MQ=255)
                                                                                                              cTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGa                             <  1:1013031/148‑1 (MQ=255)
                                                                                                              cTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGa                             <  1:1505843/148‑1 (MQ=255)
                                                                                                              cTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGa                             <  2:3317518/148‑1 (MQ=255)
                                                                                                              cTCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGa                             >  2:483257/1‑148 (MQ=255)
                                                                                                               tCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGaa                            <  2:2121911/148‑1 (MQ=255)
                                                                                                               tCAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGaa                            <  2:1834074/148‑1 (MQ=255)
                                                                                                                cAGAGGCAAAATCATCTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAAt                           >  1:1732359/1‑148 (MQ=255)
                                                                                                                            catcTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGc               >  1:1126661/1‑148 (MQ=255)
                                                                                                                             atcTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCt              <  1:1136841/148‑1 (MQ=255)
                                                                                                                              tcTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCtt             <  1:2354295/148‑1 (MQ=255)
                                                                                                                               cTATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCttt            >  2:3423477/1‑148 (MQ=255)
                                                                                                                                tATCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtg           <  2:1719929/148‑1 (MQ=255)
                                                                                                                                  tCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgtg         <  2:2543400/148‑1 (MQ=255)
                                                                                                                                  tCAGGTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTtgtg         <  2:1269352/148‑1 (MQ=255)
                                                                                                                                     ggTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCgg      <  1:1743042/148‑1 (MQ=255)
                                                                                                                                     ggTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCgg      <  1:2880181/148‑1 (MQ=255)
                                                                                                                                     ggTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCgg      <  1:1743222/148‑1 (MQ=255)
                                                                                                                                     ggTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCgg      <  1:2911600/148‑1 (MQ=255)
                                                                                                                                      gTAGAGACACAGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCggg     <  1:1832520/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                            
TTTTCGTCCGACATGTCTATAACGTGTTCAGCACTGATCGCGCTGACAAAGACCTGTGATTGCGTCGCTTTTAAGCGGCTGGCAAGCAGCCCGCGACGCTCATCATCAAGCTCAGAGGCAAAATCATCTATCAGGTAGAGACACCGCCGCCCGCTTTCACGGGTGAGGAACTCTCCTTGCGCCAGACGTAAGGCGCACATCAACAGCTTAAGCTGCCCACGCGATAAGGTATCTTCCACCGGCGCACCGTCGGCGCGAATGCGTAAGTCCGCTTTGTGCGGGCCG  >  CP009273/3873551‑3873835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑