Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,906,384 G→T 100% I215I (ATC→ATA)  glmS ← L‑glutamine:D‑fructose‑6‑phosphate aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,906,3840GT99.6% 661.3 / ‑6.0 203I215I (ATC→ATA) glmSL‑glutamine:D‑fructose‑6‑phosphate aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (139/63);  total (139/64)
Fisher's exact test for biased strand distribution p-value = 3.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 203 total aligned reads displayed.

TCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCC  >  CP009273/3906239‑3906528
                                                                                                                                                 |                                                                                                                                                
tCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTAt                                                                                                                                                >  1:2076938/1‑148 (MQ=255)
  gcgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTAttt                                                                                                                                              <  1:108339/148‑1 (MQ=255)
  gcgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTAttt                                                                                                                                              <  1:1411485/148‑1 (MQ=255)
   cgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTc                                                                                                                                             <  1:1056484/148‑1 (MQ=255)
   cgTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTc                                                                                                                                             >  2:379479/1‑148 (MQ=255)
         gCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAat                                                                                                                                       >  1:2255798/1‑148 (MQ=255)
         gCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAat                                                                                                                                       >  1:2197995/1‑148 (MQ=255)
         gCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAat                                                                                                                                       <  2:259140/148‑1 (MQ=255)
         gCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAat                                                                                                                                       <  2:3218840/148‑1 (MQ=255)
          cTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAata                                                                                                                                      <  1:755877/148‑1 (MQ=255)
          cTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAata                                                                                                                                      <  1:319996/148‑1 (MQ=255)
           tGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAatat                                                                                                                                     <  2:2126304/148‑1 (MQ=255)
             ttCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCg                                                                                                                                   >  1:445227/1‑148 (MQ=255)
             ttCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCg                                                                                                                                   >  2:2499567/1‑148 (MQ=255)
               cGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGcc                                                                                                                                 <  1:358872/148‑1 (MQ=255)
               cGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGcc                                                                                                                                 <  2:1797689/148‑1 (MQ=255)
                  aGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctc                                                                                                                              >  2:1291926/1‑148 (MQ=255)
                   gATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctct                                                                                                                             <  2:3160061/148‑1 (MQ=255)
                   gATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCctct                                                                                                                             >  1:448833/1‑148 (MQ=255)
                    aTCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCtt                                                                                                                            <  2:3331679/148‑1 (MQ=255)
                    aTCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCtt                                                                                                                            <  1:2358345/148‑1 (MQ=255)
                    aTCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCtt                                                                                                                            <  1:335640/148‑1 (MQ=255)
                     tctcTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTc                                                                                                                           >  1:2278020/1‑148 (MQ=255)
                      ctctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCa                                                                                                                          >  1:1422929/1‑148 (MQ=255)
                        ctTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAg                                                                                                                        >  2:1591729/1‑148 (MQ=255)
                             tGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGa                                                                                                                   <  2:3292446/148‑1 (MQ=255)
                              gCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGAt                                                                                                                  >  2:213872/1‑148 (MQ=255)
                              gCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGAt                                                                                                                  <  1:3033947/148‑1 (MQ=255)
                                 tGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATAGCCCTCTTAAAGGAAGATaaa                                                                                                               >  1:271122/1‑148 (MQ=255)
                                  gTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAg                                                                                                              >  2:1501785/1‑148 (MQ=255)
                                  gTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAg                                                                                                              >  1:2308862/1‑148 (MQ=255)
                                  gTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGAGTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGATTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAg                                                                                                              >  2:3243787/1‑148 (MQ=255)
                                   tAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGc                                                                                                             >  1:518917/1‑148 (MQ=255)
                                     gTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTACAAGGAAGATAAAGCGa                                                                                                           <  2:164381/148‑1 (MQ=255)
                                      tGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGAc                                                                                                          >  2:580705/1‑148 (MQ=255)
                                       gACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACg                                                                                                         >  1:926708/1‑148 (MQ=255)
                                            tAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCa                                                                                                    >  1:2509324/1‑148 (MQ=255)
                                            tAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCa                                                                                                    >  2:468821/1‑148 (MQ=255)
                                            tAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCa                                                                                                    >  1:592793/1‑148 (MQ=255)
                                              aaTGCCTTTATCGCCCGCGTCATATTGCAGTTTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCACGGAAGATAAAGCGACGGGTCAcc                                                                                                  <  2:1769895/148‑1 (MQ=255)
                                              aaTGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCAcc                                                                                                  >  2:2186505/1‑148 (MQ=255)
                                                  ccTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCa                                                                                              >  2:1316627/1‑148 (MQ=255)
                                                  ccTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCa                                                                                              >  1:1950309/1‑148 (MQ=255)
                                                  ccTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCa                                                                                              >  1:552022/1‑148 (MQ=255)
                                                     ttATCGCCCGCGTCATATTGCAGATTGGAATCGATATCCTGACGTTTTACTTCAGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTCTTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACa                                                                                           >  2:1430317/1‑148 (MQ=255)
                                                       aTCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAgc                                                                                         >  1:2869790/1‑148 (MQ=255)
                                                         cGCCCGCGTAATATTGCAGTTTGTATTCTATAACCTGACTTTTTACTTCCGCGCCAGTTTTATCTAAGTTGTTTACCTAACGGCGATTTATTTCCGCAATATCGCCATCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAgcgc                                                                                       <  2:3058952/148‑1 (MQ=255)
                                                          gCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACTTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGcc                                                                                      <  2:1754552/148‑1 (MQ=255)
                                                                gTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCTAATATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTgg                                                                                <  2:1402071/148‑1 (MQ=255)
                                                                    tatTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAg                                                                            >  2:1888602/1‑148 (MQ=255)
                                                                    tatTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCACCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAg                                                                            >  2:1915677/1‑148 (MQ=255)
                                                                     atTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGa                                                                           >  2:1003845/1‑148 (MQ=255)
                                                                       tGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAg                                                                         >  1:1763284/1‑148 (MQ=255)
                                                                         cAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCg                                                                       >  1:2421044/1‑148 (MQ=255)
                                                                           gATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGAt                                                                     >  1:1334002/1‑148 (MQ=255)
                                                                              tGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATaaa                                                                  >  1:648516/1‑148 (MQ=255)
                                                                                gATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGt                                                                >  2:1972017/1‑148 (MQ=255)
                                                                                 tttCTATATCCTGACTTTTTACTCCCGCGCATGTTTTATCTAATTTGTTTCCCGAAGGGCTTTTTATTTCCGAAATACCGCCATATACACGTAATAAAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtt                                                               <  1:1730370/147‑1 (MQ=255)
                                                                                 aTTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtt                                                               >  2:2421975/1‑148 (MQ=255)
                                                                                  ttCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGttt                                                              >  2:2806926/1‑148 (MQ=255)
                                                                                   tCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt                                                             >  1:391934/1‑148 (MQ=255)
                                                                                   tCGATATCCTGACGTTTTACGTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGtttt                                                             <  2:2562168/148‑1 (MQ=255)
                                                                                       tatCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGcc                                                         >  2:751265/1‑148 (MQ=255)
                                                                                           cTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATc                                                     >  1:53527/1‑148 (MQ=255)
                                                                                           cTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATc                                                     >  1:53539/1‑148 (MQ=255)
                                                                                               gtttttACTGCCGCGCCATTTTTATCTAAGTTGTTTACCGCACGGCGAGTTATTTCCGCAATATCGCCCTATTCATGTAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCa                                                 <  2:3246907/146‑1 (MQ=255)
                                                                                                tttttAGTTCCGCGCCTTTTTTATCTATTTTGTTTACCGAAGGGCGTTTTTTTTCCGAAATATCGCCCTCTTCAAGTAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAg                                                <  1:289788/147‑1 (MQ=255)
                                                                                                    tACTTCCGCGCCAGTTTTATCGAAGATTTTTACCGAACGGCGAGTTATTTCCGAAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCa                                            <  2:134593/148‑1 (MQ=255)
                                                                                                     aCTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCaa                                           >  1:724508/1‑148 (MQ=255)
                                                                                                        tCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATACAGTTTTCGCCCATCCCCAGGCCAATCa                                        >  2:2078244/1‑148 (MQ=255)
                                                                                                        tCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCa                                        >  2:1228315/1‑148 (MQ=255)
                                                                                                          cgcgCCTTTTTTATCTATTTTTTTTACCTACCGGCGTGTTTTTTCCGAAATATCGCCATCTTAATGTATTATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCAcc                                      <  1:2557235/148‑1 (MQ=255)
                                                                                                          cgcgCCAGTTTTATGTAATTTGTTTACCGAACGGCGATTTATTTCCGCAATATCGCCCTCTTCATGTAATATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCAcc                                      <  2:445236/148‑1 (MQ=255)
                                                                                                            cgcCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAg                                    >  1:3125899/1‑148 (MQ=255)
                                                                                                              ccAGTTTTATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCg                                  >  2:297447/1‑148 (MQ=255)
                                                                                                                  ttttATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACt                              >  1:2020210/1‑148 (MQ=255)
                                                                                                                    ttATCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTAc                            >  1:1711495/1‑148 (MQ=255)
                                                                                                                      aTCGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCa                          >  2:1698502/1‑148 (MQ=255)
                                                                                                                        cGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGa                        >  2:2545669/1‑148 (MQ=255)
                                                                                                                        cGAAGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGa                        >  2:1330172/1‑148 (MQ=255)
                                                                                                                          aaGATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAAc                      >  2:3478993/1‑148 (MQ=255)
                                                                                                                            gATGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGt                    >  1:1795109/1‑148 (MQ=255)
                                                                                                                             aTGTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTg                   >  1:1640367/1‑148 (MQ=255)
                                                                                                                               gTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgcc                 >  2:28045/1‑148 (MQ=255)
                                                                                                                               gTTTACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgcc                 >  2:28598/1‑148 (MQ=255)
                                                                                                                                tttACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgccg                >  2:512937/1‑148 (MQ=255)
                                                                                                                                  tACCGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTgccgcc              >  2:296148/1‑148 (MQ=255)
                                                                                                                                    ccGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCcag            >  2:629791/1‑148 (MQ=255)
                                                                                                                                     cGAACGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCcagc           >  1:2328345/1‑148 (MQ=255)
                                                                                                                                        aCGGCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAgg        >  2:2046497/1‑148 (MQ=255)
                                                                                                                                          ggCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGt      >  1:3184943/1‑148 (MQ=255)
                                                                                                                                           gCGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTa     >  1:3014909/1‑148 (MQ=255)
                                                                                                                                            cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGtct    >  1:1938135/1‑146 (MQ=255)
                                                                                                                                            cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGtct    >  1:2960896/1‑146 (MQ=255)
                                                                                                                                            cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTAt    >  1:129712/1‑148 (MQ=255)
                                                                                                                                            cGAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTAt    >  2:865088/1‑148 (MQ=255)
                                                                                                                                             gAGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATc   >  2:2223316/1‑148 (MQ=255)
                                                                                                                                             gAGTTATTTCCGCAATATCGCCCACTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATc   <  2:2191264/148‑1 (MQ=255)
                                                                                                                                              aGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATcc  >  2:2724602/1‑148 (MQ=255)
                                                                                                                                              aGTTATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATcc  >  2:2398724/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCGCGTTCGGCTGTTCGTAGATCTCTTTCTGCATGTAGTGACGGTAAATGCCTTTATCGCCCGCGTCATATTGCAGATTGGATTCGATATCCTGACGTTTTACTTCCGCGCCAGTTTTATCGAAGATGTTTACCGAACGGCGAGTGATTTCCGCAATATCGCCCTCTTCAAGGAAGATAAAGCGACGGGTCACCGGCAACAGCGCCAGCTGGTCAGAAGCGATAAAGTTTTCGCCCATCCCCAGGCCAATCACCAGCGGACTACCAGAACGTGCCGCCAGCAGGGTATCC  >  CP009273/3906239‑3906528

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑